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Citations for Cell Signaling Technology :
2751 - 2800 of 2863 citations for Recombinant Mouse IL2RA Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: Total protein was extracted from spheroids and organoids using lysis buffer supplemented with a protease/phosphatase inhibitor cocktail (both from Cell Signaling Technology, MA, USA). Protein concentrations were measured using a Nanodrop 2000 system (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... Lysates were then precleared by incubation for 1 hour at 4°C while rotating with 50 μL protein A agarose beads (Cell Signaling Technologies, Cat #9863) CCNT1 antibody (Cell Signaling Technologies ...
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bioRxiv - Plant Biology 2023Quote: ... The bead-bound proteins were separated by SDS-PAGE and detected by western blotting with the MBP antibody (Cell Signaling Technology, Danvers, MA, USA), His-tag antibody (Tiangen ...
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bioRxiv - Microbiology 2023Quote: ... Target proteins were detected by various primary antibodies (For phosphor-CREB rabbit mAb, Cell Signaling Technology #9198; For CREB rabbit mAb, Cell Signaling Technology #4820 ...
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bioRxiv - Immunology 2023Quote: ... Abcam Cat# ab270521).The following antibody and the concentration used against human proteins were employed: αEGFR (1:1000 for western blot; Cell Signaling Technology Cat# 4267, RRID:AB_2246311), αEGFRL858R mutant specific (1:100 microscopy ...
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bioRxiv - Genomics 2023Quote: ... The presence of the correctly tagged MYC protein was validated by Western blot probed with a c-MYC antibody (Rabbit mAb, Cell Signaling Technology, Cat# 5605). The recombinant pAAV-CRE plasmid was packaged in HEK293T cells using pHelper and pRC2-mi342 plasmids (Takara ...
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bioRxiv - Molecular Biology 2020Quote: ... The following primary antibodies were diluted in 1% milk in TBST prior to usage at the indicated concentration: mouse monoclonal α-Tubulin (1:3000, Cell Signaling Technology, #3873), rabbit monoclonal p-p70 S6K (1:1000 ...
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bioRxiv - Molecular Biology 2020Quote: ... the membranes were washed and incubated with HRP-conjugated anti-mouse or anti-rabbit secondary antibodies (1:3000, Cell Signaling Technology, #7076 or #7074) and followed by detection with ECL Western blot substrate (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2020Quote: ... phospho-eIF2α (Ser51) clone D9G8 #3398) or mouse monoclonal antibodies (eIF2α clone D-3, Santa Cruz #sc-133132, β-Actin clone E4D9Z, Cell Signaling Technology #58169 ...
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bioRxiv - Cell Biology 2020Quote: ... A polyclonal antibody against phosphorylated Tyr416 of Src and a polyclonal antibody against mouse caspase-3 were from Cell Signaling Technology (Beverly, MA, USA). α-Thrombin was purchased from Enzyme Research Laboratories (South Bend ...
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bioRxiv - Developmental Biology 2020Quote: ... stained with anti-F4/80-PE antibody (eBiosciences #12-4801-80) and isolated using an EasySep™ mouse PE positive selection kit that uses a magnetic bead-based purification strategy (Cell Signaling Technologies #17656) and in the presence of 2mM Icam4/av inhibitor peptide (Xue et al. ...
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bioRxiv - Neuroscience 2022Quote: ... Antigen detection was performed by overnight incubation with appropriate antibodies: 1:1000 rabbit monoclonal Plexin-A4 (catalog #C5D1) anti-mouse (Cell Signaling Technology, catalog #3816), 1:1000 rabbit monoclonal Nrp1 (catalog #D62C6 ...
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bioRxiv - Genetics 2019Quote: ... Secondary antibodies (a donkey anti-goat IgG-HRP antibody, Santa Cruz Biotechnology, sc-2020, and a horse anti-mouse IgG-HRP antibody, Cell Signaling Technology, #7076), conjugated to horseradish peroxidase (HRP ...
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bioRxiv - Neuroscience 2021Quote: ... was used at dilution 1:20 and incubated with the cell solution at 4°C for 30 min before staining with AlexaFlour 488 goat anti-mouse (Cell Signaling, 1:100 dilution) for another 30 min at 4°C ...
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bioRxiv - Biophysics 2020Quote: ... Cells were then resuspended in 100 μl PBS-FC containing 5% FBS with 1:100 diluted PE-conjugated anti-myc (9B11) mouse monoclonal antibody (Cell Signaling Technology, Danvers, MA) and incubated for 30 min in the dark ...
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bioRxiv - Microbiology 2021Quote: ... Immunoblot detection of phosphorylated paxillin was performed using a 1:1000 dilution of a mouse anti-phospho-paxillin (Y-118) (Cell Signaling Technology, Danvers, MA) antibody ...
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bioRxiv - Microbiology 2021Quote: ... Immunoblot detection of phosphorylated paxillin was performed using a 1:2000 dilution of a mouse anti-phospho-paxillin (Y-118) (Cell Signaling Technology, Danvers, MA) antibody ...
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bioRxiv - Biochemistry 2021Quote: ... or the H2.3 rabbit polyclonal anti-HSulf-2 antibody (dil. 1/500) followed by a horseradish peroxidase-labeled anti-mouse or rabbit IgG antibody (Cell Signaling Technology, Danvers, MA) and ImmunoStar LD (Wako Pure Chemical Industries) ...
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bioRxiv - Cancer Biology 2020Quote: ... rabbit anti-NF-κB2 p100/p52 (#4882), rabbit anti-RelB (C1E4, #4922), rabbit anti-c-Rel (D4Y6M, #12707), mouse anti-GAPDH (D4C6R, #97166) (Cell Signaling Technology, Danvers, MA); rabbit anti-H3(ab1791) ...
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bioRxiv - Plant Biology 2020Quote: ... s6k1 and tor to determine the cross-link between TOR and RPs using anti-human S6K1 antibody raised in mouse (anti phospho70kDa-S6K1-Thr(P)-389) (Cell Signaling Technologies, cat# 9206). The anti-70S6K1 (CST ...
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bioRxiv - Neuroscience 2021Quote: ... The membranes were incubated at 4°C overnight with the following mouse-specific primary antibodies: anti-NLRP3 (D4D8T, rabbit monoclonal antibody, 1:1000, #15101, Cell Signaling Technology, Danvers, MA), anti-cleaved Caspase 1 (p20 ...
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bioRxiv - Cell Biology 2021Quote: ... and a mouse monoclonal antibody against the Ser19 phosphorylated form of the myosin II regulatory light chain (P-MyoRLC) from Cell Signaling Technology (Danvers, MA). Appropriate secondary antibodies conjugated to Alexa Fluor 488 ...
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IL-27R signaling serves as immunological checkpoint for NK cells to promote hepatocellular carcinomabioRxiv - Immunology 2020Quote: ... Each membrane was washed and primary antibodies (except β-actin-HRP) were detected with a 1:5000 dilution of HRP-conjugated rabbit anti-mouse IgG (Cell Signaling Technology, 7076S) or mouse anti-rabbit IgG (Cell Signaling Technology ...
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bioRxiv - Genetics 2020Quote: ... Cells were filtered and incubated in a 1:500 solution of mouse anti-HA antibody covalently bound to Alexa 647 (Cell Signaling 6E2, product #3444). Cells were incubated with the antibody by shaking vigorously for 15-30 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit monoclonal, 1:1000), haem oxygenase-1 (HO-1; rabbit polyclonal, 1:1000) or IκBα (mouse monoclonal, 1:1000; Cell Signaling Technology, Leiden, The Netherlands) in Tris-buffer saline solution containing 0.1% Tween-20 and 5% (w/v ...
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bioRxiv - Immunology 2022Quote: ... The secondary antibodies used were Anti-rabbit IgG (H+L) (DyLight™ 800 4X PEG Conjugate) #5151 and Anti-mouse IgG (H+L) (DyLight™ 800 4X PEG Conjugate) #5257 (Cell Signaling Technology) respectively ...
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bioRxiv - Developmental Biology 2022Quote: ... The membranes were washed three times for 5 min per time with shaking then incubated with secondary antibodies (anti-mouse IgG, HRP-linked antibody, Cell Signaling, Danvers, MA, USA) diluted in 5% w/v nonfat milk at room temperature for 1 h ...
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bioRxiv - Neuroscience 2022Quote: ... blotted with mouse anti-ERK1/2 (p44/42 MAPK) and rabbit anti-phospho-ERK1/2 (p44/42 MAPK) (Cell Signaling Technologies 4696S and 4370S), both at 1:1000 in 2% BSA at room temperature for 2 hours ...
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bioRxiv - Immunology 2024Quote: ... anti-Actin and HRP- labeled goat-anti-rabbit or anti-mouse IgGs for Western blot assay were purchased from Cell Signaling Technology (Danvers, MA). Phorbol 12-myristate 13-acetate (PMA ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Membranes were incubated for 1 hour at room temperature with horseradish peroxidase-conjugated horse anti-mouse IgG solution (1:2500, Cell Signaling, Cat. No. 7074P2) diluted in 1% non-fat milk/TBST ...
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bioRxiv - Biophysics 2023Quote: ... and incubated for 1h at RT with peroxidase-conjugated secondary antibodies (anti-mouse IgG, Cell Signaling, cat. 7076; anti-rabbit IgG, Cell Signaling, cat. 7074) diluted 1:5000 in 5% non-fat dried milk in TBS-T solution ...
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bioRxiv - Cancer Biology 2023Quote: ... The membranes were then incubated with horseradish peroxidase-conjugated secondary antibodies (anti-rabbit IgG at 1:5000 dilution or anti-mouse IgG at 1:5000 dilution; both from Cell Signaling Technology, Danvers, MA), and the bound proteins were visualized using a chemiluminescence-based detection kit (ImmunoStar Zeta ...
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bioRxiv - Cancer Biology 2023Quote: ... 1:4700 in 5% non-fat milk (anti-rabbit IgG #ADI-SAB-300-J, Enzo Lifesciences, Farmingdale NY or anti-mouse IgG #7076, Cell Signaling Technology, Danvers MA). The membranes were imaged using Azure Biosystems Radiance Plus substrate solution and an Azure c600 Imaging System.
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bioRxiv - Cell Biology 2023Quote: ... Anti-EGFR (D38B1) XP® Rabbit mAb #4267 and anti-EGFR Mouse mAb (IP Specific) #2256 were purchased from Cell Signaling Technology (Danvers, MA). The anti-β-actin antibody was purchased from Abcam (Cambridge ...
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bioRxiv - Neuroscience 2023Quote: ... the membrane was blocked in 5% nonfat milk in PBST (PBS and 0.1% TWEEN20) and incubated overnight at 4°C with primary antibodies mouse anti-GFP Living Color (Cell Signaling Technology 2955, 1:1000) to detect TDP-43 YFP and rabbit anti-Beta-Actin (Cell Signaling Technology 4967S ...
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was blocked with 5% BSA diluted in TBS-Tween0,1% for 1 hour and primary antibodies were incubated overnight (rabbit anti-HER2 #29D8 Cell Signaling; mouse anti-vinculin #A250291). Donkey anti-mouse IgG or goat anti-rabbit IgG secondary antibody conjugated to horse-radish peroxidase (Jackson ImmunoResearch Laboratories ...
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bioRxiv - Biochemistry 2023Quote: ... The expression of MBP-FoxP3ΔN was validated by Western blot using Mouse MBP Tag antibody(8G1) (Cell Signaling Technology, Cat#2396, 1:2000 dilution).
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bioRxiv - Neuroscience 2023Quote: ... was incubated overnight at 4°C followed by corresponding horseradish peroxidase (HRP)-linked anti-mouse (Cell Signaling Technology, 7076V, 1:2000 dilution in TBST) or anti-rabbit (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2024Quote: ... we incubated the cells with fluorescent secondary FAB fragments raised to the species corresponding to the primary antibody for 1 hr in PBS supplemented with 10% FBS (either Anti-rabbit IgG Fab2 Alexa Fluor ® 555 or Anti-mouse IgG Fab2 Alexa Flour ® 488, both Cell Signaling Technology, Catalogue # 647 4414 S and # 4408 S ...
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bioRxiv - Molecular Biology 2021Quote: ... The same membrane that was used to detect AIRE protein was washes and reused in an incubation with an anti-GAPDH rabbit polyclonal primary antibody (Cell Signaling Technology, Beverly, MA, USA) following incubation with peroxidase conjugated anti-rabbit antibody.
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bioRxiv - Cancer Biology 2020Quote: ... Protein amounts of OP18 and beta-actin were quantified using a primary stathmin polyclonal antibody (1:1000; Cell Signaling Technology, NEB GmbH, Frankfurt/Main, Germany) and a polyclonal beta-actin antibody (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were incubated with primary antibody in 10 % Donkey Serum in PBS overnight at 4 °C: Phospho-S6 Ribosomal Protein (Ser240/244) Rabbit mAb (Cell Signaling Technology, Cat# 5364; RRID:AB_10694233). After HSCs were washed three times with PBS + 0.1 % Tween-20 ...
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bioRxiv - Cell Biology 2021Quote: ... Forty-five μg of protein were resolved in SDS-PAGE and blotted with anti-phospho-eNOS (Ser1177) antibodies (Cell Signaling, Danvers, MA, USA, 1:250), followed by incubation with goat anti-rabbit horseradish peroxidase secondary antibodies (Jackson ImmunoResearch ...
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bioRxiv - Plant Biology 2022Quote: ... The extracts were then incubated at 28°C and the samples were collected at the indicated intervals for determination of GST and GST-WL1 protein abundance by western blots using the anti-GST (#2625, Cell Signaling Technology, 1:1000 dilution) and alkaline phosphatase-conjugated anti-rabbit (# 31340 ...
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bioRxiv - Developmental Biology 2021Quote: ... 20 μg of protein per lane were subjected to SDS-PAGE and probed with the following primary antibodies: YAP (Cell Signaling Technologies #8418, 1:500), YAP/TAZ (Cell Signaling #4912 ...
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bioRxiv - Neuroscience 2020Quote: ... Astrocytes were then collected by scraping and the CNMCS Compartmental Protein Extraction Kit (BioChain Cat# K3013010 Hayward, CA) with protease/phosphatase inhibitors (PI, Cell Signaling, Cat# 5872S, Danvers, MA) was used and the nuclear fractions were isolated for each treatment condition ...
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bioRxiv - Immunology 2020Quote: ... Antibodies used to detect proteins involved in glycolysis (Hk1 clone C35C4, Glut1 clone D3J3A and HIF-1a D1S7W) were from Cell Signaling (all at 1:500) were used to intracellular staining ...
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bioRxiv - Systems Biology 2022Quote: ... Immunoblot analysis was used to assess the adequacy of nuclear purification by measuring Lamin A/C (rabbit, 1:1000, Cell signaling # 2032, a nuclear protein).
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bioRxiv - Neuroscience 2023Quote: Primary antibodies to the following proteins were used at the indicated concentrations: ubiquitin (1:1000, P4G7, BioLegend; 1:200 Cell Signaling; 1:200, Z0458, Dako), GABARAP (1:1,000 ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein lysates were extracted at 3 hours post-treatment and analysed by western blot following overnight incubation with antibodies against the following primary proteins: Vinculin (E1E9V, rabbit, Cell Signaling Technology, 13901, 1:1000), Phosphorylated p44/42 MAPK (ERK1/2 ...