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Citations for Cell Signaling Technology :
2701 - 2750 of 2863 citations for Recombinant Mouse IL2RA Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... Blotted nitrocellulose membranes were incubated with primary antibodies (mouse -p53, SC-126, Santa Cruz Biotechnology; rabbit -CTCF, Millipore 07-729; rabbit -GAPDH, cst-2118, Cell Signaling Technology) followed by detection with HRP conjugated secondary antibodies (Jackson Immunoresearch Laboratories) ...
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bioRxiv - Immunology 2021Quote: ... The cells were washed twice with PBS and horse-anti-mouse-IgG-conjugated to HRP was added (1:2000 in 1% milk in PBS, Cell Signaling Technology, S7076) at room temperature for 45 min ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated at 4°C for 18-22 hr in a cocktail comprising anti-GSK3β mouse monoclonal antibody (1:1500; #9832, Cell Signaling, Rockford, IL), anti-phospho GSK3β (Ser9 ...
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bioRxiv - Cell Biology 2022Quote: ... 0.1% Tween-20 in 1x TBS) and then treated with primary antibodies (YAP/TAZ, anti-rabbit, Cell Signaling, 8418 and beta-Actin, anti-mouse, Cell Signaling, 3700) overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... for 2h then incubated with primary antibodies (1:5000) overnight: anti-HA antibody (rabbit polyclonal antibody; Sinobiological #11684-T62) and anti-β-actin antibody (mouse monoclonal antibody; Cell Signaling #3700). The membrane was washed with TBS – Tween 20 0.1% ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies diluted in 5% skim milk in TBST: horse anti-mouse IgG (1:20,000, Cell Signaling Technology, cat# 7076), donkey anti-sheep IgG (1:20,000 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... mouse anti-GAPDH (D4C6R; #97166), rabbit anti-FAS (#3189), and rabbit anti-XBP-1s (D2C1F, #12782S) were obtained from Cell Signaling (Danvers, MA). Mouse anti-calnexin (C8.B6 ...
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bioRxiv - Physiology 2024Quote: ... The rabbit monoclonal anti STAT1 phospho-Tyr701 antibody (clone 58D6, #9167) and the anti α-tubulin mouse monoclonal antibody (clone DM1A, mAb #3873) were purchased from Cell Signaling Technology. Anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Neuroscience 2024Quote: ... mouse anti-otoferlin (Developmental Studies Hybridoma Bank, HCS-1) diluted at 1:100 and rabbit anti-cleaved caspase-3 (Cell Signaling Technology, 9661) diluted at 1:250 ...
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bioRxiv - Neuroscience 2024Quote: ... and 2 h later they were labeled with mouse anti-Iba1 (1:500; Fujifilm Wako Chemicals, VA, USA) or rabbit anti-p75NTR (1:750; Cell signaling, MA, USA) overnight ...
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bioRxiv - Cell Biology 2024Quote: ... followed by incubation for 1 hour at room temperature in the dark in with secondary antibodies (Anti-rabbit: IgG DyLight 800, Anti-mouse: IgG DyLight 680 (Cell Signaling Technologies, USA)) ...
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bioRxiv - Biochemistry 2023Quote: ... were used at a concentration of 1:1000 and visualized using HRP-conjugated secondary antibodies (anti-rabbit [Cell Signaling, 7074S] or anti-mouse [Cell Signaling, 7076S]) at a concentration of 1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated overnight at 4°C in 0.2% BSA/TBS with the following antibodies: anti-Myc mouse IgG (Cell Signaling, cat. No. 2276) for mDGKε-Myc staining and anti-STIM1 rabbit IgG (Cell Signaling ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibodies were detected using goat anti-mouse or anti-rabbit IgG (H+L) Alexa fluor 488 or 647 (Cell signaling Technologies/ Bioconcept) secondary antibodies diluted 1:500 in 1% BSA 0.1% Triton-X 100 in PBS for 3 hours in the dark at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... Cat # 69295) at (1:1000), anti-Rab27B (E4V30, Cat # 17572), and α-Tubulin Mouse mAb (DM1A, Cat#3873) all purchased from Cell Signaling Technology. The anti-Rab27B (Cat # 13412-1-AP ...
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bioRxiv - Cell Biology 2024Quote: ... β-actin [8H10D10] primary antibodies and anti-rabbit-HRP or anti-mouse-HRP antibody were purchased from Cell Signaling Technology (Danvers, MA, USA). IRF3 [A19717] were purchased from ABclonal (Woburn ...
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bioRxiv - Cell Biology 2024Quote: ... The membrane was washed by 1× TBST 3 times and incubated with a secondary anti-rabbit-HRP or anti-mouse antibody-HRP (Cell Signaling, Danvers, MA) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... To confirm the specificity of staining cells were incubated with isotype mouse (7076P2) or rabbit (7074P2) IgG antibodies (Cell Signaling, Danvers, MA, USA) (Fig ...
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bioRxiv - Immunology 2023Quote: ... and intracellular location of 4H2 or IgG control was detected by immunostaining with an Alexa Fluor 488-conjugated goat anti-mouse IgG antibody (Cell Signaling, Danvers, MA) as previously described (32) ...
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bioRxiv - Molecular Biology 2023Quote: ... Blots were washed in TBS containing 0.1 % Tween20 and labeled with horseradish peroxidase (HRP)-conjugated secondary anti-mouse or anti-rabbit antibody (Cell Signaling Technology, Beverly, MA). Proteins were enhanced by chemiluminescence (Amersham ECL plus Western Blotting detection system ...
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bioRxiv - Cell Biology 2023Quote: ... phospho-ERK1/2 (D13.14.4E; Cat. #4370) and mouse antibodies again ERK1/2 (L34F12; Cat. #4696) and β-Actin (8H10D10; Cat. #3700) are from Cell Signaling Technology. Rabbit anti-Cytokeratin 10 (Cat ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were then washed three times with TBST and incubated in secondary antibodies accordingly: anti-mouse HRP-linked IgG (Cell Signaling Technology, 7076S) or anti-rabbit HRP-linked IgG (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... the membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies (anti-mouse-HRP, Cell Signaling #7076; anti-rabbit-HRP, Cell Signaling #7074) in blocking solution (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... The plates were washed three times with PBST and incubated with either 1 μg/ml of rabbit monoclonal anti-mouse TREM2 (Cell Signaling Technology, 76765) for full length Trem2 ELISA (ELISA 1) ...
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bioRxiv - Genomics 2023Quote: ... Western blotting was performed according to standard procedures using the following antibodies and dilutions: TP53 (DO-1, 1:500, Cell Signaling #18032, mouse), CDKN1A (F-5 ...
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bioRxiv - Cancer Biology 2023Quote: ... membranes were treated with an HRP-linked secondary antibody (Anti-rabbit IgG cat. no. 7074S or Anti-mouse IgG cat. no. 7076S; Cell Signaling Technology, Inc) for 2 hours at room temperature ...
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bioRxiv - Genomics 2023Quote: ... Cells were proceeded for IF as described above using anti-CD56 mouse antibodies (1:10, Coulter #PN4235479-G) and anti-TCF7L2 rabbit antibodies (1:100, Cell Signaling Technology #C48H11), revealed by Cy3-coupled anti-mouse IgGs and Cy5-coupled anti-rabbit IgGs.
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bioRxiv - Biochemistry 2023Quote: ... The bound primary antibodies were detected with an HRP-conjugated anti-mouse immunoglobulin-G at 1:5000 dilution (Cell Signaling Technology, Catalog# 7076S) by chemiluminescence (VWR) ...
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bioRxiv - Cancer Biology 2023Quote: ... These membranes were subsequently probed with the indicated primary antibodies and incubated with appropriate goat anti-rabbit IgG or goat anti-mouse IgG (Cell Signaling Technology, USA) secondary antibodies conjugated with horseradish peroxidase (HRP ...
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bioRxiv - Neuroscience 2023Quote: ... Doubly phosphorylated (active) ERK1/2 was detected with a phospho-ERK-specific [phosphor-ERK1/2(Thr202/Tyr204)] mouse monoclonal antibody (Cell Signaling, cat.# 9106) at 1:2,000 dilution ...
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bioRxiv - Neuroscience 2023Quote: ... blots were washed in 1x TBST and incubated with appropriate HRP-conjugated secondary (Goat anti-rat 7077, Cell Signaling, Goat anti-mouse 7076, Cell Signaling, Goat anti-rabbit 7074, Cell Signaling) for two hours with 2% milk powder in TBST ...
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bioRxiv - Neuroscience 2023Quote: ... Normalization was carried out on the control group and related to the corresponding ß-actin (monoclonal mouse, Cat. No. #3700, dilution 1:2000 in blocking solution, Cell Signaling Technology and monoclonal rabbit ...
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bioRxiv - Microbiology 2023Quote: ... expression of hACE and cACE2 was validated via immunofluorescence and immunoblots through detection of the c-terminal myc tag with a 1:500 dilution of antibody Myc-Tag-9B11 Mouse mAb (Cell Signaling Technology, 2276).
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bioRxiv - Molecular Biology 2023Quote: ... the membranes were incubated with peroxidase-conjugated secondary antibodies (Anti-rabbit IgG, HRP-linked Antibody, Cell Signaling #7074; Anti-mouse IgG, HRP-linked Antibody, Cell Signaling #7076) and developed with a luminol-based enhanced chemiluminescence (ECL ...
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bioRxiv - Neuroscience 2023Quote: ... The membrane was then washed in TBS-Tween three times for 10 min at RT and incubated with the secondary antibody anti-mouse-HRP (1:5000) (Cell Signaling, Danvers, MA) for one hour at RT ...
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bioRxiv - Genetics 2021Quote: ... and probed for phosphorylated EIF2α protein levels with rabbit anti-Eif2a S51 phosphorylation polyclonal antibody (1:500 dilution) (Cell Signaling Technologies, cat. number D9G8) and Total EIF2α protein levels with rabbit anti-EIF2a polyclonal antibody (1:10,000 dilution ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibodies to all native and/or phosphorylated proteins (see Supplementary Table S1 for details) except for WT1 were purchased from Cell Signaling Technology (Boston, MA). The C-terminal anti-WT1 antibody (C-19 ...
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bioRxiv - Cancer Biology 2021Quote: ... Western blot was performed as described before and the following antibodies were used for protein detection: β-tubulin (Cell Signaling Technology, β-Tubulin (9F3) Rabbit mAb #2128 ...
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bioRxiv - Cancer Biology 2021Quote: ... A fraction of each sample was then precleared with protein A and salmon sperm DNA-bound agarose beads (Cell Signaling Technologies, Cat. No 9863), for 1 hour in 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... A fraction of each sample was then precleared with protein A and salmon sperm DNA-bound agarose beads (Cell Signaling Technologies, Cat. No 9863), for 1 hour in 4°C ...
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bioRxiv - Microbiology 2020Quote: ... of the sample was incubated with 3 μg of antibody (or 3 μg of IgG as control) and 30 μl of protein G magnetic beads (Cell Signaling Technology, Cat. 7008) at 4 °C overnight ...
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bioRxiv - Cancer Biology 2021Quote: ... A fraction of each sample was then precleared by incubation with protein A and salmon sperm DNA-bound agarose beads (Cell Signaling, Cat. No 9863), for 1 hour in 4oC ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated for 48 hr at 4°C with antibodies against phosphorylated S6 ribosomal protein (Ser240/244, 1:800, Cell Signaling Technology, catalog # 5364S), GFP (1:1000 ...
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bioRxiv - Neuroscience 2019Quote: ... anti-Microtubule-associated proteins 1A/1B light chain 3B (LC3B-I/-II) at 1:1000 dilution (14, 16 kDa, Cell Signaling Technology, Danvers, MA); anti-monocarboxylate transporter 1 (MCT1 ...
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bioRxiv - Cancer Biology 2019Quote: ... In order to positively identify the proteins, the following antibodies were used: MAN1B1 (SC-100543, 1:500) and actin (1:2000) (Cell Signaling Technology, Beverly, MA). Horse radish peroxidase (HRP)-conjugated secondary antibodies were incubated with the membrane at RT for 1 hr ...
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bioRxiv - Cell Biology 2021Quote: ... The following primary antibodies were used for protein immunoblots: Anti p-Tyr (4G10, Millipore Sigma, #05-321 and P-Tyr-1000, Cell Signaling Technology, #8954), anti V5-HRP (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were separated on a 4–12% SDS-PAGE gel followed by standard immunoblot analysis using anti–CISH (Cell Signaling, Clone D4C10, 1:2000) and Vinculin (Cell Signaling ...
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bioRxiv - Cancer Biology 2022Quote: ... we validated the presence of the correctly sized tagged MYC protein and absence of wild type MYC by Western blot probed with a c-MYC antibody (Rabbit mAb, Cell Signaling Technology, Cat# 5605). We selected homozygous clone number 2 (for N-BioTAP-cMYC ...
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bioRxiv - Neuroscience 2022Quote: ... Anti-ionized calcium-binding adaptor molecule 1 (Iba-1, FUJIFILM Wako Chemicals USA, Richmond, VA) and anti-glial fibrillary acidic protein (GFAP, Cell Signaling Technology, Danvers, MA) antibodies were diluted to 1:1,000 and used to incubate tissue sections overnight at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... The expression levels of target proteins were assessed by western blotting (Figure S1) with antibodies against ADA (clone D1P4Y, Cell Signaling Technology, Cat#65184), ADSSL1 (clone 2D12 ...