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Citations for Cell Signaling Technology :
2451 - 2500 of 2863 citations for Recombinant Mouse IL2RA Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Nanobodies were incubated for 1 h at RT and detected using Mouse-anti-Myc antibody (1:1000, 9B11 clone, Cell Signaling). US28 was visualized with the rabbit-anti-US28 antibody (1:1000 ...
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bioRxiv - Immunology 2020Quote: ... The membranes were then washed and incubated with anti-mouse or anti-rabbit HRP-conjugated secondary antibodies from Cell Signaling Technology and GE Healthcare ...
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bioRxiv - Developmental Biology 2021Quote: ... and 0.05% Tween 20] plus 5% BSA before incubation with the primary antibody (1:1000 for mouse monoclonal 12C11 anti-REST and 1:2000 for anti-LaminB1 rabbit polyclonal, Cell Signaling). Anti-rabbit IgG or anti-mouse IgG conjugated to horseradish peroxidase (1:2000 ...
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bioRxiv - Cell Biology 2019Quote: ... which was used at a 1:50 dilution in combination with a secondary anti-mouse HRP antibody (1:10,000, Cell Signaling #7076S). ECL Select (GE Healthcare ...
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bioRxiv - Cell Biology 2019Quote: ... The membranes were then washed and incubated with either mouse or rabbit secondary antibody linked with horseradish peroxidase (Cell Signaling Technologies) and washed ...
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bioRxiv - Bioengineering 2020Quote: ... HRP conjugated goat anti-mouse (1:4000) and goat anti-rabbit (1:4000) secondary antibodies were obtained from Cell Signaling Technology, Danvers ...
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bioRxiv - Biochemistry 2019Quote: ... cells were washed three times in PBS and then processed following manufacturer’s instructions for DuoLink In Situ Detection Reagents Red Mouse/Rabbit (DUO92101) using rabbit anti-HA (Cell Signaling Technology) and mouse anti-FLAG (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2019Quote: ... anti-Mena (1:300, mouse monoclonal, a kind gift from Frank Gertler) and anti-Cre Recombinase (1:300, Cell Signaling 12830). Secondary antibodies used were goat anti-mouse or anti-rabbit IgG conjugated to Alexa Fluor-488 ...
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bioRxiv - Cell Biology 2021Quote: The following antibodies (in 5% milk in TBST) were used for western blotting: anti-E-cadherin (#14472, monoclonal mouse, 1:1000, Cell Signaling), anti-N-cadherin (#BDB610920 ...
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bioRxiv - Systems Biology 2020Quote: ... from femur by incubating the samples with mouse anti-Runx-2 (an osteoblast marker) antibody (1:1000 dilution in PBS containing 0.5% BSA, Cell Signaling Technology) at 4 °C ...
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bioRxiv - Systems Biology 2020Quote: ... [37] was performed incubating the samples with mouse anti-PLIN1 antibody (1:1000 diluition in PBS containing 0.5% BSA, Cell Signaling Technology).
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TRANSITION OF PODOSOMES INTO ZIPPER-LIKE STRUCTURES IN MACROPHAGE-DERIVED MULTINUCLEATED GIANT CELLSbioRxiv - Cell Biology 2020Quote: ... The mouse anti-E-cadherin mAb (24E10) and rabbit anti-Myosin IIa polyclonal antibody (34035) were from Cell Signaling (Danvers, MA). The rat anti-SIRPα/CD172a polyclonal antibody (P84 ...
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bioRxiv - Biochemistry 2021Quote: ... Membranes were washed with PBS-T and incubated with HRP-coupled goat anti-mouse or anti-rabbit IgG antibodies (Cell Signaling). Detection was performed with Clarity Lightning ECL (Bio Rad ...
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bioRxiv - Cell Biology 2022Quote: ... anti-rabbit immunoglobulin G (IgG) (H+L) (Dylight 800 conjugates, Cat. #5151) and anti-mouse IgG (H+L) (Dylight 680 conjugates, Cat. #5470) (Cell Signaling) were used as secondary antibodies ...
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bioRxiv - Biochemistry 2022Quote: ... The membranes were then probed with goat anti-rabbit and goat anti-mouse secondary antibodies (#7074, #7076, Cell Signaling, Danvers, MA), and blots were incubated with Clarity Western ECL Substrate (Cat ...
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bioRxiv - Molecular Biology 2022Quote: ... The membrane was then transferred to a secondary antibody solution containing a 1:5000 dilution of α-mouse HRP (Cell Signaling) for one hour of shaking at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... and 1:5000 dilution of HRP-conjugated rabbit anti-mouse secondary antibody (Cat. No. 7076, Lot No. 36, Cell Signaling Technology), and the blot was developed similarly.
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bioRxiv - Immunology 2022Quote: ... NFκB phospho (rabbit-anti-NFκB p65 total, Cell Signaling Technology #8242; mouse anti-NFκB p65 p-Ser536, Cell Signaling Technology #13346), mixed with Lumit BiT Set 2 (anti-mouse Ab-SmBiT ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were incubated in the appropriate horseradish peroxidase (HRP)-conjugated secondary antibodies (FosB anti-rabbit HRP, GAPDH anti-mouse HRP, Cell signaling) at 1:500 for 1 hr at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... The membrane was immunoblotted with primary antibody followed by either Anti-mouse IgG HRP-linked Antibody (Cat # 7076S, Cell Signaling Tech) or Anti-rabbit IgG HRP-linked antibody (Cat #7074S ...
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bioRxiv - Cell Biology 2022Quote: ... and HRP-linked anti-Rabbit IgG and HRP-linked anti-Mouse IgG (Cell Signaling, cat# 7074 and 7076; WB 1:1000). IF – immunofluorescence ...
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bioRxiv - Neuroscience 2022Quote: ... Any APP antibodies remaining on the cell surface were quenched with a non-florescent goat anti-mouse secondary antibody conjugated to horseradish peroxidase (Cell Signaling) diluted in 10% goat serum (Invitrogen ...
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bioRxiv - Cancer Biology 2022Quote: ... The secondary antibodies were as follows: horseradish peroxidase (HRP)-conjugated goat anti-mouse immunoglobulin (IgG) (1:10,000, 7076, Cell Signaling Technology), HRP-conjugated goat anti-rabbit IgG (1:10,000 ...
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bioRxiv - Immunology 2024Quote: ... Primary antibody was removed by several washes with TBS + 0.1% Tween-20 and then subjected to incubation with secondary antibody (anti-mouse-HRP, 7076S, Cell Signaling Technology). The detection of protein bands was then carried out using enhanced chemiluminescence (ECL ...
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bioRxiv - Microbiology 2023Quote: ... TMPRSS2 expression was evaluated using SimpleWestern Abby (ProteinSimple, San Jose, CA, USA) with an anti-Myc tag mouse monoclonal antibody (Cell Signaling Biotechnology ...
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bioRxiv - Cell Biology 2024Quote: ... following incubation with the primary antibodies anti-p53 (1:500, mouse, (Ab-6) Pantropic OP43 Sigma-Aldrich) and anti-GAPDH (1:1000, rabbit, 14C10 Cell Signaling) for 1h at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... Anti-mouse IgG-horseradish peroxidase (HRP) (NA931; Cytiva, Tokyo, Japan) and anti-rabbit IgG-HRP (#7074; Cell Signaling Technology, MA, USA) were used as the secondary antibodies.
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bioRxiv - Molecular Biology 2023Quote: The following antibodies were used in this study: mouse monoclonal anti-β-actin (Cell Signaling Technology; 3700; RRID: AB_2242334; LOT# 20), rabbit polyclonal anti-SARS-CoV-2 nsp2 (GeneTex ...
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bioRxiv - Neuroscience 2023Quote: ... cells were resuspended in 250 μL1% BSA in 1X PBS containing Myc-tag Mouse monoclonal antibody (Cell Signaling, 9B11, Cat.#2276) with a ratio of 1:500 ...
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bioRxiv - Physiology 2023Quote: ... Protein expression was measured by standard western blot procedures using anti-mouse primary antibodies against Arf6 (rabbit monoclonal; 1:1000; 19kDa; Cell Signaling), β-actin (mouse monoclonal ...
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bioRxiv - Physiology 2023Quote: ... Membranes were washed three times with TBS-T for 5 min and incubated with peroxidase-linked anti mouse or rabbit IgG at a 1:10,000 dilution in TBS-T (#7076/#7074S, Cell Signaling, Germany) for one hour at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Respective expression was assessed by immunostaining using a mouse anti-HA antibody (1:500; Cell Signaling#2367, RRID AB_10691311 and a rabbit anti-Flag (1:500; Cell Signaling#14793 RRID AB_2572291 followed by Cy5-conjugated donkey anti-mouse (1.9 µg/ml ...
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bioRxiv - Neuroscience 2022Quote: ... and then overnight at 4°C with the primary antibody (mouse anti-β-ACTIN, 1:30,000, Sigma; rabbit anti-AKT1, 1:1000, Cell Signaling; rabbit anti-AKT2 ...
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bioRxiv - Biochemistry 2022Quote: ... Blots were then washed three times with wash buffer and incubated 75 min at room temperature with a 1:10,000 dilution of goat anti-mouse HRP conjugated antibody (Cell Signaling Technology). After three washes with wash buffer ...
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bioRxiv - Neuroscience 2023Quote: ... Trem2 was immunoprecipitated with either a Rabbit monoclonal antibody raised against the carboxy-terminus of mouse Trem2 (Cell Signaling Technology, 76765,) referred to as αTrem2-CT ...
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bioRxiv - Cell Biology 2023Quote: ... 1:2,000 for IB) and mouse anti-Myc-tag (cat. #2276, RRID: AB_331783; 1:500 for IB) were purchased from Cell Signaling Technology. Rabbit anti-calnexin (cat ...
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bioRxiv - Cancer Biology 2023Quote: ... Secondary antibody staining employed anti-mouse IgG and anti-rabbit IgG HRP-linked antibodies (Cell Signaling Technologies, #7076 and #7074, respectively) were used at 1:5000 dilution ...
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bioRxiv - Molecular Biology 2023Quote: Mouse anti-MYC (2276), rabbit anti-HA (3724) and rabbit anti-FAM134B (83414S, for immunoprecipitation) were purchased from Cell Signaling Technology. Rabbit anti-FLAG (F3165) ...
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bioRxiv - Immunology 2023Quote: ... stimulated T cells were fixed with the Foxp3/TF staining buffer set (eBioscience) for 30 min at 4°C and stained intranuclearly with rabbit polyclonal anti-mouse HIF-1α (Cell Signaling) o/n in permeabilization buffer (eBioscience) ...
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bioRxiv - Cancer Biology 2023Quote: ... The primary antibodies were probed with 1:1000 Alexa Fluor 488 conjugated anti-mouse IgG (Cell Signaling Technology, cat. No. 4408S), Alexa Fluor 594 conjugated anti-rabbit IgG (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2023Quote: ... The primary antibodies were probed with 1:1000 Alexa Fluor 488 conjugated anti-mouse IgG (Cell Signaling Technology, cat. No. 4408S), Alexa Fluor 594 conjugated anti-rabbit IgG (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2022Quote: ... then incubated in HRP-linked anti-mouse or anti-rabbit IgG antibody for 1h at 24°C with gentle rocking (1:5,000 dilution, Cell Signaling Technology). Membranes were washed in TBST and protein bands were visualized using the Pierce ECL detection system (Cat #32106 ...
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bioRxiv - Cancer Biology 2023Quote: ... and then incubated with horse radish peroxidase-linked anti-mouse or anti-rabbit secondary immunoglobulin antibodies (1:10000; Cat# 7076S, Cat# 7074S Cell signaling) in PBST for 40 mins ...
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bioRxiv - Cell Biology 2023Quote: ... to the following proteins were used for the immunodetection of proteins using horseradish peroxidase-conjugated anti-mouse (Cytiva, NA931) and anti-rabbit (Cytiva, NA934) secondary antibodies (1:1,000): CHOP (Cell Signaling, 5554), Cleaved Caspase-3 (Cell Signaling ...
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bioRxiv - Biochemistry 2023Quote: ... Primary antibodies were detected with an HRP-conjugated anti-mouse immunoglobulin-G at 1:5000 dilution (Cell Signaling Technology, Catalog# 7076S) for ZIP4 or an HRP-conjugated anti-rabbit immunoglobulin-G for β-actin at 1:5000 dilution (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were washed in PBS and incubated with fluorescent secondary antibody Goat anti-mouse Alexafluor 594 (8890, Cell signaling technology, USA). Membranes were mounted on slides with Fluoromount-G containing DAPI (00-4959-52 ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were then incubated with horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (1:5000, CS7076, Cell Signaling, Danvers, MA, USA) and goat anti-rabbit IgG (1:5000 ...
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bioRxiv - Cancer Biology 2023Quote: ... each membrane was probed with the appropriate horseradish peroxidase-conjugated secondary antibody (anti-mouse dilution 1:5,000; GTX213112-01, Gene Tex; anti-rabbit dilution 1:3000; 7074S, Cell Signaling Technology), and incubated for 1□h at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... Secondary antibody was either goat anti-rabbit (7074) or horse anti-mouse (7076) IgG conjugated with horseradish peroxidase (Cell Signaling Technology). Staining was developed with SuperSignal West Dura Extended Duration Substrate (Thermo Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... The primary antibodies used in this study were 1:2,500-diluted mouse anti-β-actin (Wako, #017-24551) and 1:1,000-diluted rabbit anti-cleaved caspase-3 (Cell Signaling, #9661). The membrane was washed three times with PBS for 10 minutes and incubated with secondary antibodies at room temperature for 1 hour ...