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Citations for Cell Signaling Technology :
2151 - 2200 of 2312 citations for 2 4 Methyl 5 thiazolyl ethyl decanoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Membranes were blocked with TBS containing 1 % casein and 2 % BSA followed by incubation with primary antibodies (survivin 1:2000, 71G4B7, Cell Signaling; beta actin 1:5000 sc-4778 ...
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bioRxiv - Microbiology 2023Quote: ... SARS-CoV-2 spike protein-specific IgG was measured using SARS-CoV2 spike protein serological IgG ELISA kit (Cell Signaling Technology) per manufacturer’s instructions.
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bioRxiv - Microbiology 2024Quote: ... Protein concentration was quantified by BCA assay and 200 μg of protein was incubated with 2 μg/ml of primary antibody or with isotype control (rabbit IgG or mouse IgG isotype controls; Cell Signaling). Protein-antibody complexes were incubated overnight with constant rotation at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... at room temperature for 2 hrs in blocking buffer and treated with horseradish peroxidase (HRP) conjugated Goat anti-Rabbit IgG secondary antibody (Cell Signaling) or Goat anti-mouse IgG secondary antibody respectively for 1 h at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... every 8th section of the brain was processed for double-fluorescent immunostaining for Ku80 and one of the following: Microtubule-associated protein 2 (MAP2; 8707, Cell Signaling), doublecortin (ab18723 ...
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bioRxiv - Neuroscience 2024Quote: ... followed by 2 h blocking with 15% milk powder (in TBST) and overnight incubation with rabbit anti-BIP (3183, Cell signaling, 1/1000 for retina ...
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bioRxiv - Microbiology 2024Quote: ... The following antibodies were used for viral protein analysis: SARS-CoV-2 anti-Spike and anti-N protein (cat #569996 and #33336, respectively, Cell Signaling) and anti-β-actin (Sigma – cat #a2228) ...
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bioRxiv - Cancer Biology 2024Quote: ... anti-phospho Myosin Light Chain 2 (Ser19) (pMLC2) (#3671 from Cell Signaling Technology, 1:1000 for IB; 1:200 for IF); anti-phospho Cofilin-1 (Ser3 ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed with a FACS buffer (FACS solution supplemented with 4% fetal calf serum) and incubated with anti-rabbit secondary antibody Alexa Fluor 488™ (#4412, Cell Signaling Technology, Danvers, MA, USA)(1:800 ...
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bioRxiv - Neuroscience 2020Quote: Membranes were then incubated 24-48 hours at 4°C in primary antibodies – PERK (1:500 anti-Rbt; Cell Signaling Technologies [CST], Danvers, MA; cat # 3192S), IRE-1α (1:500 anti-Rbt ...
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bioRxiv - Neuroscience 2021Quote: ... The immunodetection of selected proteins was performed overnight at 4 °C with the following antibodies: p62 (Cell Signaling Technology Inc, Danvers, MA, USA, 5114, 1:1000), LC3b (Abcam ab51520 ...
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bioRxiv - Molecular Biology 2021Quote: ... for 1 h at room temperature and incubated with primary antibodies overnight at 4 °C with anti-Cd9 ([1:3000; System Biosciences, Palo Alto, CA, USA] and anti-Gapdh [1:5000; Cell Signaling, Davers, MA, USA; 14C10]).
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bioRxiv - Cell Biology 2023Quote: ... 1 μg/μl (500 μg protein) samples were prepared and incubated overnight at 4 °C in primary antibody against acetyl-lysine (Cell Signaling; Cat No. 9814 (1:100)) ...
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bioRxiv - Microbiology 2022Quote: ... the cells were washed twice with the blocking buffer and incubated at 4 °C with primary antibodies (mouse anti-Strep, Qiagen, Cat #: 34850, 1: 150 dilution) (rabbit anti-PDI, Cell Signaling, Cat #: 3501, 1: 200 dilution) (rabbit anti-Flag ...
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bioRxiv - Microbiology 2022Quote: ... the blot was incubated at 4 °C with primary antibodies (rabbit anti-Flag, Cell Signaling, Cat #: 14793, 1:3,000 dilution) (rabbit anti-Calnexin, Cell Signaling, Cat #: 4691, 1:2,000 dilution) (rabbit anti-COX4 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The membranes were probed overnight at 4 °C with the specified primary antibodies at the dilution of 1:1000 (Cell Signaling Technology: RIPK1 Rabbit mAb#3493)) or 1:4000 (β-actin Rabbit mAb#4970)) ...
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bioRxiv - Cancer Biology 2024Quote: ... then incubated with primary antibody diluted in 1% FBS/PBS overnight at 4°C (MAP2, Cell Signaling # 4542, 1:500 dilution; NEFH, Cell Signaling # 55453, 1:500 dilution). After 3 washes with PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.1% Tween® 20 Detergent) for 1h and incubated over-night with primary anti-H3K9Ac antibody at 4°C (C5B11, 9694T, Cell Signaling Technology, Danvers, MA, USA). Membranes were incubated for 1 hour with secondary anti-rabbit HRP-conjugated antibody (NA934 ...
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bioRxiv - Physiology 2021Quote: ... which was blocked with 5% BSA in PBS containing 0.1% Tween-20 (0.1% PBST) and incubated with rabbit anti-pAkt antibody (Cell Signaling Technology, 4060S, 1:1,000 dilution) or rabbit anti-AKT antibody (Cell Signaling Technology 9272S ...
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bioRxiv - Cancer Biology 2021Quote: ... blocked in 5% BSA in TBS-T and probed with primary antibodies recognizing β-Actin (Cell Signaling Technology, #3700, 1:4000, Danvers, MA, USA), c-Myc (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2022Quote: ... The following primary antibodies were added after blocking for an hour with 5% non-fat milk: STAT3 and Tyr705 pSTAT3 (Cell Signaling Technology, MA, USA), GAPDH (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2021Quote: Cells were fixed with 0.25% paraformaldehyde in ice-cold PBS for 5 min and incubated with anti-ERp57 (#A484, Rabbit mAb, Cell Signaling Technology, Danvers, MA, USA) 1:100 for 40 minutes ...
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bioRxiv - Biophysics 2020Quote: ... Cells were then resuspended in 100 μl PBS-FC containing 5% FBS with 1:100 diluted PE-conjugated anti-myc (9B11) mouse monoclonal antibody (Cell Signaling Technology, Danvers, MA) and incubated for 30 min in the dark ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was washed with 1X TBST (3 × 5 min) and then incubated with respective secondary antibodies (Horse anti-mice 1:2000; Horse anti-rabbit 1:2000, (Cell signaling technology, MA, USA) and developed by using ECL chemiluminescent reagent (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were blocked in 5% BSA in TBS-T before incubation with primary antibodies: phospho-AMPKα Thr172 (1:1000, Cell signaling, MA, USA #2535), β-actin (1:1000 ...
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bioRxiv - Cancer Biology 2020Quote: ... After saturation with TBS Tween 5% BSA for 1h and hybridization of the membranes with primary overnight (BCR-ABL Ab3 Calbiochem, pCrkL Tyr207 Cell Signaling, pTYR 4G10 Millipore) and secondary 1h antibodies coupled to HRP ...
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bioRxiv - Neuroscience 2020Quote: ... Tissue was blocked for 2-hours in 1% NGS and 0.3% Triton X-100 followed by 2-3 days of 4C° incubation in 1xPBS (5% NGS, 0.3% Triton X-100) with primary antibody(ies): pS6 S235-236 (Cell Signaling, Catalog: 4858, 1:500), pS6 S244-247(ThermoFisher ...
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bioRxiv - Pathology 2020Quote: ... Membranes were blocked with 5% bovine serum albumin (BSA) in PBS and incubated with anti-APRT antibody or an anti-β- Actin antibody (Cell Signaling Technology, USA) at 4°C overnight followed by the corresponding secondary antibody for 1 hr at room temperature ...
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FGF signalling is involved in cumulus migration in the common house spider Parasteatoda tepidariorumbioRxiv - Developmental Biology 2021Quote: ... Fluorescent pMad antibody staining was performed using the Phospho-Smad1/5 (Ser463/465) (41D10) Rabbit mAb (Cell Signaling Technology, Inc.; antibody concentration: 1:1000) as primary and an Alexa488 anti rabbit AB (Invitrogen ...
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bioRxiv - Physiology 2021Quote: ... the membranes were washed 3 times in TBS-T for 5 minutes and incubated for 1 hour in HRP-conjugated anti-rabbit antibodies (Cell Signaling Technology, London, UK) at dilution of 1:5000-1:10,000 ...
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bioRxiv - Physiology 2023Quote: ... blots were washed with TBS-T three times and then incubated in TBS-T containing 5% non-fat dry milk and anti-biotin horseradish peroxidase (Cell Signaling, Danvers, MA, USA) and HRP-conjugated donkey anti-chicken antibody (Sigma-Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: 5 μM thick paraffin-embedded liver sections were used for hematoxylin and eosin (H&E) staining and Ki67 (Cell signaling technology 12202S, 1:400) immunohistochemical staining ...
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bioRxiv - Developmental Biology 2022Quote: ... The membranes were washed three times for 5 min per time with shaking then incubated with secondary antibodies (anti-mouse IgG, HRP-linked antibody, Cell Signaling, Danvers, MA, USA) diluted in 5% w/v nonfat milk at room temperature for 1 h ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were incubated overnight at 4°C with antibodies for the designated antigens INTEGRIN α5 (1:1000, #4705), INTEGRIN β3 (1:1000, #4702), β-ACTIN(1:1000, #8457), β-TUBULIN (1:1000, #2146) purchased from Cell Signaling Technology, GAPDH (1:1000 ...
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bioRxiv - Cancer Biology 2023Quote: ... blocked in Tris-buffered saline/0.1% Tween (TBST)/5% milk and probed with antibodies against E-Cadherin (Cell Signaling Technology, 3195S, 1:1000 dilution), Vimentin (Cell Signaling Technology ...
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bioRxiv - Genomics 2023Quote: ... The blots were washed with 1X TBST (6 washes of 5 min each) followed by incubation with HRP-conjugated anti-rabbit secondary antibody (Cat. No. 7074S, Cell Signaling Technology, MA, USA) for 1 h at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... The membranes were washed with PBS 3 times for 5 minutes and then incubated with horseradish peroxidase conjugated secondary antibody (Cell Signaling Technology, 7074, 7076) in 5% non-fat milk in PBST at room temperature for 1 hour ...
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bioRxiv - Neuroscience 2024Quote: ... the membrane was blocked with milk-TBST (5% skimmed milk, 1xTBS and 0.1 % Tween-20) and then incubated with anti-STAT1 (Cell Signaling Technology Cat# 9172, RRID:AB_2198300), anti-pSTAT1 (Cell Signaling Technology Cat# 9167 ...
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bioRxiv - Bioengineering 2024Quote: ... Samples were blocked in a 5% nonfat milk solution and stained for the common EV marker CD9 using a primary antibody (Cell Signaling Technologies, Cat. # 13174) and an anti- rabbit HRP secondary antibody (Cell Signaling Technologies ...
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bioRxiv - Physiology 2024Quote: ... membranes were washed 3 times for 5 minutes and incubated for 1 hour in HRP-conjugated anti-rabbit antibody (Cell Signaling Technology, London, UK) at a dilution of 1:20,000 in non-fat dry milk ...
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bioRxiv - Neuroscience 2024Quote: ... 0.1% Tween-20 and 5% bovine serum albumin) containing each of the following primary antibodies: dynamin related protein-1 (DRP1, 1 μg/ml, Cell Signaling Technology, Danver, MA), phosphorylated DRP1 (Ser616 ...
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bioRxiv - Systems Biology 2024Quote: ... cells were first incubated with a blocking buffer for 1 hour at room temperature (1x PBS/ 5% normal serum/0.3% TritonX-100) followed by overnight incubation with the primary antibody against phospho-ERK (Cell Signaling Technology, catalog no. 4370S) at 1:800 dilution at 4 °C ...
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bioRxiv - Biochemistry 2024Quote: ... Anti- mouse, Cat. No. 7076; 1: 10 000 in 5% skim milk/TBS-T, Anti-rabbit, Cat. No. 7074S, Cell Signaling Technology, Massachusetts, US) and Pierce ECL Western Blotting Substrate (Cat ...
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bioRxiv - Plant Biology 2020Quote: ... membranes were incubated with the antibody against Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® (Cell Signaling Technology, USA). As a loading control membranes were incubated with anti-tubulin antibody (Abiocode ...
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bioRxiv - Immunology 2021Quote: ... The proteins were then blotted overnight with anti-human phospho-Akt and anti-human phospho-Erk1/2 mAbs (Cell Signaling, MA, USA), followed by anti-rabbit or anti-mouse horseradish peroxidase(HRP)-conjugated secondary mAb (ThermoFisher scientific ...
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bioRxiv - Developmental Biology 2020Quote: ... Cells were permeabilized with 0.1% Triton-X and stained for intracellular phospho-p44/42 Erk1/2 Thr202/Tyr204 at 1:700 dilution (Cell Signaling Technology, 4370) and p44/42 Erk1/2 at 1:700 dilution (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2022Quote: ... and the pellet containing the DOC-insoluble material was resuspended in 100 μl SDS solubilization buffer, pH8.8 (20 mM Tris-Cl, 2 mM EDTA, 1% w/v SDS, 1X protease inhibitor cocktail (Cell Signaling Technology, 5871). The DOC-insoluble pellet was thoroughly dissolved by heating the sample to 95 °C and vortexing ...
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bioRxiv - Neuroscience 2021Quote: ... Then the cells were blocked in 10% donkey serum for 2 h and incubated with primary antibodies (goat anti-Iba1, 1:400, Abcam; mouse anti-CD68, 1:400, Cell Signaling Technology; rabbit anti-Arg-1 ...
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bioRxiv - Developmental Biology 2020Quote: ... Nuclei pellets were washed twice with 200 μL of Dig-wash buffer before resuspending in 100 μL Dig-wash buffer supplemented with 2 mM EDTA and a 1:50 dilution of H3K27ac primary antibody (Cell Signaling, 8173) and incubated overnight at 4°C on an overhead rotator ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-ERK1/2 (dilution 1:2000) and rabbit α-tubulin (dilution 1:2000) all purchased from Cell Signaling Technology (Danvers, MA). The phospho-ERK1/2 expression was evaluated first using a 680-nm secondary anti-mouse antibody ...