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Citations for Cell Signaling Technology :
2051 - 2100 of 2312 citations for 2 4 Methyl 5 thiazolyl ethyl decanoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... Zebrafish embryos were stained with primary antibodies directed against Phospho-p44/42 MAPK (Erk1/2) Thr202/Tyr204 (4370S, Cell Signaling) at 1:200 ...
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bioRxiv - Systems Biology 2023Quote: ... Constant volumes of sample were incubated overnight with 2 μg α-BRD4 antibody (Cell Signaling, Danvers, MA, USA, Cat. # 13440) or a nonspecific isotype control (LSBio ...
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bioRxiv - Systems Biology 2023Quote: ... The following primary antibodies were used: i) Phospho-Myosin Light Chain 2 (Ser19) (1:50, Rabbit, Cell Signaling Technology #3671S) ii ...
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bioRxiv - Cell Biology 2023Quote: ... The remaining supernatants were subjected to immunoprecipitation by incubation with 2 μl of anti-mTOR antibody (#2983, Cell Signaling Technology) for 3 hours (4 °C ...
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bioRxiv - Immunology 2023Quote: ... cells were blocked in 2% BSA-PBS and incubated with NF-κB p65 (1:100, Cell Signaling Technology, Cat# 6956S) followed by Alexa Fluor 488 conjugated chicken anti-mouse IgG (1:300 ...
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bioRxiv - Biophysics 2023Quote: ... The following primary antibodies were used: mouse anti-phospho-myosin light chain 2 (S19) (Cell Signaling 3675S, 1:100 dilution), mouse anti-E-cadherin (BD Biosciences 610181 ...
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bioRxiv - Plant Biology 2022Quote: ... and probed with anti-phospho-p44/42 mitogen-activated protein kinase (Erk1/2) (Thr202/Tyr204) (Cell Signaling; 1:1,000 dilution), anti-MBP (New England Biolab ...
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bioRxiv - Cell Biology 2022Quote: ... phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (#4370) and pS473-Akt (#4060) antibodies were from Cell Signaling Technology (CST). Further antibodies used were against GST (sc-138 ...
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bioRxiv - Plant Biology 2022Quote: ... The primary antibody was anti-p-ERK (anti-pERK MAPK [Phospho-p44/p42 MAPK, ERK1/2, Thr202/Tyr204, D13.14.4E], Cell Signaling Technology) that specifically recognizes the [T-X-Y] MAPK activation motif ...
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bioRxiv - Cell Biology 2023Quote: ... a rabbit monoclonal antibody phospho-p44/42 MAPK (Erk1/2, Thr202/Tyr204; cat no. 4376, Cell Signaling Technology, MA, USA) to detect phospho-Slt2 ...
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bioRxiv - Neuroscience 2023Quote: ... and goat anti-mouse IgG (H+L) F(ab’)2 fragment conjugated to Alexa Fluor 488 (Cell Signaling Technology, #4408). Each well ...
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bioRxiv - Developmental Biology 2024Quote: ... and 100 mM 2-beta mercaptoethanol) and re-probed with anti-β-actin monoclonal antibody (1:2000, Cell Signaling Technology) as the internal control.
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bioRxiv - Immunology 2024Quote: ... Sections were then incubated with a primary antibody specific for YAP/TAZ (PBS with 2% BSA, 93622 Cell Signaling Technology) for 2 hours at RT ...
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bioRxiv - Molecular Biology 2024Quote: ... The cell pellets were resuspended in Stain Buffer (2% FBS in DPBS) with Rabbit anti-HA (Cell signaling technology, 3724S) antibody diluted at 1:500 ...
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bioRxiv - Cancer Biology 2024Quote: ... Blots were developed using a Chemidoc-touch digital imager and band intensity quantified using ImageJ (https://imagej.net/ij/index.html).The antibodies used for Western blot included: p44/42 MAPK (ERK1/2) (Cell Signaling Technologies), Phospho-p44/42 MAPK (Erk1/2 ...
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bioRxiv - Biochemistry 2020Quote: ... Free-floating sections were blocked with 5% donkey serum for 1.5 hours before overnight incubation at 4°C with primary antibodies (goat anti-Albumin antibody, Abcam ab19194, 1:100 dilution; rabbit anti-V5 antibody, Cell Signaling Technology, 1:500 dilution). Sections were washed ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were incubated with primary antibody in 10 % Donkey Serum in PBS overnight at 4 °C: Phospho-S6 Ribosomal Protein (Ser240/244) Rabbit mAb (Cell Signaling Technology, Cat# 5364; RRID:AB_10694233). After HSCs were washed three times with PBS + 0.1 % Tween-20 ...
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bioRxiv - Cancer Biology 2022Quote: ... The membranes were blocked with non-fat milk at room temperature for an hour and incubated overnight at 4°C with a primary antibody: anti-P2RX4 (Cell Signaling Technology, Cat# 70659, RRID:AB_2799789) and anti-P2RX7 (Cell Signaling Technology ...
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bioRxiv - Immunology 2022Quote: ... Membrane was blocked using 1x Roti®Block (Carl Roth) for one hour and incubated over night at 4 °C with the primary antibodies against cGAS (Cell Signaling Technology, #31659, 1:1000), β-actin (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2022Quote: ... the membranes were incubated overnight at 4°C with gentle agitation in primary antibody dilution buffer: phosphorylated ALK (Y1604) (Cell Signaling Technologies, #3341, 1:1000), ALK (Cell Signaling Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were detected from the blots using the following primary antibodies diluted in the appropriate ratio with 1x TBST and incubated overnight at +4°C: rabbit anti-phospho-Akt1 (S473, 1:1000, #9018, Cell Signaling Technology, Danvers, MA, USA), rabbit anti-phospho-Akt2 (S474 ...
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bioRxiv - Neuroscience 2023Quote: ... The membranes were blocked with 5% dry milk in TBS and incubated overnight at 4 °C with specific monoclonal antibody anti-p-p38 MAPK (Thr180/Tyr182) (1:2000, Cell Signaling, Danvers, MA, USA, 4511) and anti-actin (1:7000 ...
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bioRxiv - Cell Biology 2024Quote: ... Primary antibodies were dissolved with 3% BSA in tris buffered saline (TBST) and membranes were incubated overnight at 4°C with SQSTM1/p62 (Cell Signaling, cat# 88588, 1:1000). The primary LC3 A/B (Cell Signaling ...
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bioRxiv - Neuroscience 2024Quote: ... after which the sections were incubated overnight at 4°C with rabbit anti-cFos antibody (1:6400 dilution, #2250, Cell Signaling Technology, Danvers, MA, USA), 1% NHS ...
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bioRxiv - Immunology 2024Quote: ... and kept at 4 °C for up to 10 days until chromatin fragmentation using SimpleChIP® Plus Sonication Chromatin IP Kit (Cell Signaling Technology, Danvers, MA). Briefly ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibodies (mouse, anti-claudin-4, ThermoFisher, cat: 32-9400, at 1:200 dilution; rabbit, anti-GM130, Cell signaling, cat: 12480, at 1:1600 dilution) were incubated (BSA at 2% ...
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bioRxiv - Physiology 2023Quote: ... 5% low-fat dry milk (blocking solution) and incubated overnight at 4°C with a rabbit monoclonal anti-Numb antibody (#2756, Cell Signaling Technology, Inc., Danvers, MA) at 1:1000 dilution in 5% BSA in TBS-T ...
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bioRxiv - Neuroscience 2023Quote: ... 4 serial brain sections from each animal were stained with rabbit anti-human Aβ antibody (clone D12B2; Cell Signaling Technology Europe, Frankfurt am Main, Germany) and Cy3-conjuagted goat anti-rabbit IgG (Jackson ImmunoResearch Europe Ltd ...
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bioRxiv - Neuroscience 2022Quote: ... stored as free-floating sections in phosphate buffered saline at 4°C and then incubated with a rabbit antibody against cFos (1:1000, Cell signaling #2250, Danvers, MA, USA) for 48 h ...
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bioRxiv - Microbiology 2024Quote: ... and cell extracts were incubated for 3 h at 4°C with beads pre-coupled overnight with rabbit anti-LC3C (Cell Signaling Technology, 14736, 1:250) antibody ...
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bioRxiv - Molecular Biology 2024Quote: ... and probed overnight at 4°C on a shaker with mouse anti-Ty (Bastin et al., 1996) and rabbit anti-HA (Cell Signaling Technologies, cat. no. 3724S). Membranes were then incubated with goat anti-mouse IgG conjugated to HRP (VWR ...
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bioRxiv - Molecular Biology 2024Quote: ... Sections were then blocked with 5% goat serum/2.5% BSA for at least 1 hour before overnight incubation at 4°C with an antibody cocktail consisting of rabbit anti-vimentin (1:100) (catalog #: 5741; Cell Signaling Technologies; Danvers, MA, USA), mouse anti-dystrophin IgG2b (1:50 ...
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bioRxiv - Biochemistry 2024Quote: ... The membranes were blocked using 5% non-fat milk in TBS-0.05% tween20 (TBST) and incubated overnight at 4 °C with primary antibodies against P-Rex1 and GAPDH (Cell signaling technology #13168 and #5174 respectively). The membranes were washed three times with TBST and then incubated with secondary antibodies in blocking solution for 2 h at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... and then incubated overnight at 4°C with PANX1 CT-412 (Penuela et al., 2007; Penuela et al., 2009) or HA (Cell Signaling 3724, RRID:AB_1549585, Danvers, MA) primary antibody in a humidity chamber ...
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bioRxiv - Bioengineering 2024Quote: ... The membrane was incubated overnight at 4°C under rotation with the following primary antibodies diluted in TBST (1:1000): mouse anti-CD44 (Cell Signaling Technology, Boston, MA, USA); rabbit anti-MMP2 (Cell Signaling Technology ...
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bioRxiv - Bioengineering 2021Quote: ... washed three times for 10 min in PBS and incubated for 1 hour in blocking solution (5% normal goat serum (Cell Signaling Technology #5425), 0,3% Triton X-100 in PBS ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were washed 4 times in TBS-T for 5 min at room temperature after which they were incubated with HRP-conjugated secondary antibodies (Cell Signaling, Danvers, MA) for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were washed three times for 5 min with PBS and incubated in anti-mouse IgG Alexa Fluor® 555 Conjugate (Cell signaling #4409) at a dilution of 1:500 for 1 hour ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were washed 4 times in TBS-T for 5 min at room temperature after which they were incubated with HRP-conjugated secondary antibodies (Cell Signaling, Danvers, MA) for 1 h at room temperature ...
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bioRxiv - Developmental Biology 2020Quote: ... Histological sections (5 mm) were stained for immunohistochemistry using a standard protocol with antibodies for LC3A/B (1:200; #12741S; Cell Signaling Technology, USA).
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bioRxiv - Neuroscience 2020Quote: ... The membranes were blocked with 5% skimmed milk and incubated with primary antibodies (rabbit anti-PPAR-γ antibody, Cell Signaling Technology, 1:1000) and HRP-conjugated goat anti-rabbit IgG ...
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bioRxiv - Neuroscience 2020Quote: ... sections were washed with Bond Wash solution 2x 10s and blocked with a phosphate-buffered saline (PBS) solution containing 5% normal goat serum (5425S, Cell Signaling, Danvers, MA) and 0.5% TritonX-100 (T8787 ...
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bioRxiv - Biochemistry 2021Quote: ... boiled in sample buffer for 5 min and subjected to immunoblot assay with anti-HA antibody (Cell Signaling Technology, Inc., #3724, Danvers, MA).
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bioRxiv - Synthetic Biology 2023Quote: ... Primary staining was performed on separate blots with the following antibody dilutions in 5% BSA/TBST: rabbit anti-EZH2 1:1000 (Cell Signaling Technology, 5246S), rabbit anti-CBX2 1:500 (Bethyl Laboratories ...
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bioRxiv - Cancer Biology 2023Quote: ... Immunoprecipitates with anti-lamin A/C antibody were analysed in parallel with 5% unbound supernatants by immunoblotting with anti-lamin A/C (Cell Signaling; Cat# #4777) or anti-His (Cell Signaling ...
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bioRxiv - Cancer Biology 2023Quote: ... then probed overnight at 4°C with primary antibodies diluted in a 5% BSA/ 1X TBS-0.1% Tween 20 (TBS-T) solution using either Vinculin (E1E9V) (Cell Signaling, Cat. No. 13901) diluted 1:2000 ...
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bioRxiv - Neuroscience 2023Quote: ... For live cell microscopy cells were untreated or treated with 5 μM Aβ fibrils (as described above) pre-incubated with an Aβ antibody (Cell Signaling Technologies, #15126) conjugated to an Alexa Fluor 555 secondary (Invitorogen) ...
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bioRxiv - Bioengineering 2022Quote: ... The membranes were blocked with PBS with 0.1% Tween-20 (PBST) with 5% Blotto for one hour and then incubated with antibodies to phospho-p90RSK (Thr359/Ser363; #9344; Cell Signaling Technology, Inc.) and RSK1/RSK2/RSK3 (32D7 ...
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bioRxiv - Physiology 2023Quote: ... was heated at 90°C for 5 minutes and loaded into acrylamide gels before incubated with primary antibodies against catalase (CAT, #14097, Cell Signaling, 1:5000), 3-nitrotyrosine (3-NT ...
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bioRxiv - Cancer Biology 2023Quote: ... Each blot was then washed thrice in 1X TBST for 5 minutes and proteins were detected using either LumiGLO/Peroxide Reagent or SignalFire™ ECL reagent (7003 and 6883, respectively, Cell Signaling Technology) and an iBright™ imaging system (ThermoFisher) ...