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Citations for Cell Signaling Technology :
2101 - 2150 of 2312 citations for 2 4 Methyl 5 thiazolyl ethyl decanoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... The membrane was blocked for 1 h at room temperature with 5 % BSA dissolved in TBS-Tween (0.1 %) and incubated with the primary antibody rabbit anti-phospho-threonine [(Cell Signaling Technology®, #9381) 1:1000] ...
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bioRxiv - Bioengineering 2023Quote: ... The blots were washed three times for 5 min each with TBS-T then incubated in SignalFire ECL Reagent (Cell Signaling Technology, 6883) for 1 min and imaged using a BioRad ChemiDoc imager.
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bioRxiv - Cancer Biology 2023Quote: ... Immunoprecipitates with anti-lamin A/C antibody were analysed in parallel with 5% unbound supernatants by immunoblotting with anti-lamin A/C (Cell Signaling; Cat# #4777) or anti-His (Cell Signaling ...
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bioRxiv - Physiology 2023Quote: ... was heated at 90°C for 5 minutes and loaded into acrylamide gels before incubated with primary antibodies against catalase (CAT, #14097, Cell Signaling, 1:5000), 3-nitrotyrosine (3-NT ...
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bioRxiv - Cancer Biology 2023Quote: ... Each blot was then washed thrice in 1X TBST for 5 minutes and proteins were detected using either LumiGLO/Peroxide Reagent or SignalFire™ ECL reagent (7003 and 6883, respectively, Cell Signaling Technology) and an iBright™ imaging system (ThermoFisher) ...
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bioRxiv - Neuroscience 2024Quote: ... Blocking was performed with 5% non-fat milk dissolved in TBST for 1 hour followed by incubation with primary antibodies PRKN (Cell Signaling Technology, 2132) at 1:2000 dilution in 5% BSA in TBST at 4 °C overnight and VCL (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... and blocked with 1x TBST + 5% normal goat serum (NGS) cocktail for 60 min at RT before applying the NFκB primary antibody (Cell Signaling, 6956, 1:1000) in 1x TBST cocktail containing 1% bovine serum albumin (BSA ...
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bioRxiv - Physiology 2024Quote: ... The PVDF membrane was incubated with 5% w/v non-fat dry milk or 5% BSA for 1 h at room temperature and immunoblotted with anti-HSL (phospho-serine 660) (1:1000, Cell Signaling Technology, 45804S), anti-HSL (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... Fixed cultures were permeabilized with 0.1% Triton-x in PBS 10 minutes and blocked in blocking buffer (5% Normal Goat Serum (Cell Signaling Tech. cat#: 5425S) in 1x PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... membranes were washed 3×5 minutes in TBST and incubated for 1 hour with HRP-conjugated anti-rabbit IgG (diluted 1:2000 v/v in TBST with 5% BSA; Cell Signaling, cat #: 7074). Membranes were finally washed 3×5 minutes in TBST ...
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bioRxiv - Immunology 2024Quote: ... The membranes were blocked with PBS-Milk 5% for 30 min at RT before staining with primary antibody against HA-tag (C29F4, Cell signaling, 1:500) and Actin (ab49900 ...
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bioRxiv - Biochemistry 2024Quote: ... and proteins of interest were analyzed using primary antibodies (1:1000 in 5% BSA in TBS-T) as follows: anti-LKB1 (Cell Signaling Technology; 3050S), anti-phospho-LKB1 (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2024Quote: ... The membrane was then blocked for 1hr in 5% skim milk in TBST and incubated in primary mouse monoclonal antibody Lamin A/C 4C11 (Cell Signaling Technology #4777) in 5% BSA overnight at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... #2983), Histone H3 (1:10,000, #4499), MEK1/2 (1:1000, #8727) and LDH (1:5000, #2012;) were from Cell Signaling Technology, Inc ...
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bioRxiv - Cancer Biology 2021Quote: ... Other commercially available primary antibodies used were: anti-Phospho-p44/42 MAPK (phosphorylated ERK1/2, Thr202/Tyr204, Cell Signaling Technology #4377), anti-p44/42 MAPK (ERK1/2 ...
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bioRxiv - Cell Biology 2020Quote: ... Rabbit antibodies for anti-myosin-light-chain-2 (Cat #3672) and anti-phospho-myosin-light-chain-2 (raised against phospho-Thr18/Ser19; Cat #3674) were purchased from Cell Signaling and used at 1:500 (Western blot) ...
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bioRxiv - Cell Biology 2022Quote: ... then seeded in DMEM/F12 and 1% serum plus antibiotic-antimycotic solution 48 hrs post-transfection cells were blocked and treated with Ab against α5β1 (Novus, 2-52680) and αv (Cell Signaling 4711) at 10ug/ml for 30 minutes prior to cell surface stripping (0.2M acetic acid ...
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bioRxiv - Immunology 2021Quote: ... followed by washing and 2 hour incubation with goat-anti-rabbit-HRP antibody at 1:5000 (all from Cell Signaling Technology). Membranes were washed again and detected with SuperSignal West Pico Chemiluminescent substrate (Thermofisher) ...
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bioRxiv - Plant Biology 2021Quote: ... the membrane was incubated overnight with the primary phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (Cell Signaling Technology, #9101). Subsequently ...
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bioRxiv - Developmental Biology 2020Quote: ... blocked and incubated with an antibody recognizing Myosin Light Chain 2 phosphorylated at serine19 (1:200, rabbit; Cell signaling technology, #3671), or with the anti-tyrosinated tubulin antibody YL1/2 (1:50 ...
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bioRxiv - Cell Biology 2022Quote: ... 10-15 μg of proteins were separated by SDS-PAGE and immunoblotted using the following antibodies: p-ERK1/2 (9101, Cell Signaling), ERK1/2 (4695 ...
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bioRxiv - Cell Biology 2021Quote: ... the membrane was incubated with antibody that recognizes proteins IDH 1 (1:1000) and IDH 2 (1:1000) (Cell Signaling, MA) and α-Tubulin (Abcam ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were washed with TBST prior to incubation for 2 hrs at room temperature in the appropriate secondary antibody (anti-rabbit or anti-mouse; Cell Signaling) at 1:2000 dilutions ...
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bioRxiv - Genetics 2022Quote: ... Beads were then resuspended in 250 μl blocking solution and 2 μg antibody (RAD21, Abcam ab992; CTCF, Cell Signaling Technology 3418) was added ...
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bioRxiv - Genetics 2020Quote: ... and bound Myc bead (1/2 total amount) samples were analyzed by SDS-PAGE and immunoblotting with rabbit anti-Myc monoclonal antibody (1:2000; Cell Signaling) to detect Myc-tagged EXOSC5 and EXOSC2 ...
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bioRxiv - Microbiology 2020Quote: ... 250 μL of lysate were incubated with 2 μL of mouse Myc-Tag antibody clone 9B11 (Cell Signaling Technology, Cat. 2276S) for 4 h with rotation at 4°C ...
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bioRxiv - Bioengineering 2020Quote: ... 1 x 108 induced cells were stained with 1 µM of directly AF488-labeled SARS-CoV-2 RBD and 0.5 µM anti-HA AF647 (Cell Signaling Technology) antibody ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Anti-rabbit IgG (H+L)-F(ab’)2 Fragment-Alexa Fluor 555 Conjugate (cat. No. 4413, Cell Signaling Technology, Beverly, USA), CRLS1 pAb (cat ...
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bioRxiv - Pathology 2020Quote: ... for one hour at 25°C and incubated overnight with the rabbit anti-phospho-MEK1/2 (Ser217/221) antibody (Catalog #9154; Cell Signaling), the mouse anti-MEK1/2 antibody (Catalog #4694 ...
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bioRxiv - Cell Biology 2020Quote: ... rabbit polyclonal anti-phospho-myosin light chain 2 (Thr18/Ser19) (for immunostainings on elastomers; 1:200, Cell Signaling; cat. no. #3674). An anti-Plexin-B1 antibody was raised against the extracellular domain of Plexin-B1 ...
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bioRxiv - Physiology 2021Quote: ... were subjected to western blot analysis with antibodies against c-Jun NH(2)-terminal kinase (JNK) and phospho-JNK (Cell Signaling). Immunocomplexes were detected using the Licor Odyssey secondary detection system.
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bioRxiv - Cancer Biology 2021Quote: ... the degree of staining of the tissue specimens was observed under the Zeiss Apotome.2 microscope (20X objective, 0.8 NA) after DAB staining (Cell Signaling Technology) and hematoxylin (IHC World ...
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bioRxiv - Systems Biology 2020Quote: ... from femur by incubating the samples with mouse anti-Runx-2 (an osteoblast marker) antibody (1:1000 dilution in PBS containing 0.5% BSA, Cell Signaling Technology) at 4 °C ...
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bioRxiv - Biochemistry 2021Quote: ... Primary antibody solution mix contains two primary antibodies raised in two different species (mouse and rabbit) targeting the proteins of interest: rabbit anti-mitofusin 2 (1:50, mAb D2D10, Cell Signaling), rabbit anti-VDAC (1:200 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were stained with rabbit antibodies against phospho (S473)-AKT (p-AKT) and phospho (Thr202/Tyr204) p44/42 ERK1/2 (p-ERK) (4060, 9101; Cell Signaling) for 30 min ...
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bioRxiv - Cancer Biology 2021Quote: ... the slides were blocked with 3% BSA and incubated with primary antibodies against pERK1/2 (1:500, Cell Signaling Technology, 4370) or cleaved Caspase-3 (1:400 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and rabbit antibodies specific for p-Erk1/2 (phospho-p44/42 MAPK) (1:1000, #4370; Cell Signaling Technology, Beverly, MA, USA), Erk1/2 (p44/42 MAPK ...
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bioRxiv - Cancer Biology 2022Quote: ... and incubated with the primary antibodies: polyclonal rabbit antibody against PTP1B (2 μg/mL; #5311; Cell Signaling Technology, Boston, MA, USA) and monoclonal mouse antibody against Cdk3 (2 μg/mL ...
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bioRxiv - Plant Biology 2022Quote: ... Phosphorylated MAPK proteins were detected by immunoblot analysis with antiphospho-p44/42 MAPK (Erk1/2)(Thr202/Tyr204) (D13.14.4E) rabbit mAb (Cell Signaling Technology, USA). The blotted membrane was stained with Coomassie Brilliant blue (CBB ...
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bioRxiv - Microbiology 2022Quote: ... After washing 3 times cells were blocked with 2% BSA for 1h at 37 followed by incubation with 1/100 dilution of anti-Flag monoclonal antibody (Cell Signaling) for 1.5h at RT ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was blocked with 5% non-fat dry milk in TBST and incubated with primary antibodies against Perilipin-2 (1:5000 gift from Dr. Ronal Kuhnlein) 13 and Actin (1:1000, Cell Signaling). The western blot images were captured by a BIO-RAD workstation.
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bioRxiv - Immunology 2023Quote: ... Cells were blocked with 2% BSA in PBS for 1 hour at room temperature before antibody staining with rabbit ⍺-Sec61b (Cell Signaling) and mouse ⍺-Tom20 (Santa Cruz ...
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bioRxiv - Neuroscience 2024Quote: ... rabbit monoclonal anti-phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (1:2000, Cell Signaling Technology, Danvers, MA, USA,; RRID: AB_2315112), rabbit polyclonal anti-human SOD1 (1:1000 ...
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bioRxiv - Microbiology 2024Quote: ... They were passed through a 0.45 µm filter and precleared with 2 μg/ml anti-rabbit isotype IgG antibody (Cell Signaling #2729) with protein A/G magnetic beads (Pierce ...
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bioRxiv - Molecular Biology 2023Quote: ... Double resistant cell lines with dual integration of 4EBP1/2 shRNAs were validated by western blot (Cell Signaling Technology #9452, #2845) for efficient stable knockdown of the targeted proteins.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Immunolabeling was realized using the rabbit monoclonal primary antibody anti-phospho-ERK1/2 Thr202/Tyr204 (#4370, Cell Signaling Technologies, 1:2,000), or anti-total ERK1/2 (#05-1152 ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then permeabilized and blocked with 0.1% saponin/0.1% BSA in PBS before incubated with α-SARS-CoV-2 Nucleocapsid primary antibody (Cell Signaling Technology) at 1:1000 in permeabilization/blocking buffer overnight at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... anti-phospho-Akt (Thr308) (C31E5E) and anti-phospho-p44/42 MAPK (Erk 1/2) (Thr202/Tyr204) (E10) antibodies (Cell Signaling Technology). Each experiment was repeated 4 times ...
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bioRxiv - Immunology 2023Quote: ... Membranes were blocked with TBS containing 1 % casein and 2 % BSA followed by incubation with primary antibodies (survivin 1:2000, 71G4B7, Cell Signaling; beta actin 1:5000 sc-4778 ...
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bioRxiv - Microbiology 2023Quote: ... SARS-CoV-2 spike protein-specific IgG was measured using SARS-CoV2 spike protein serological IgG ELISA kit (Cell Signaling Technology) per manufacturer’s instructions.