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Citations for Cell Signaling Technology :
1651 - 1700 of 10000+ citations for Dengue Virus Serotype 2 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Membranes were washed in 1xPBS and incubated with anti-rabbit IgG (H+L) F(ab’)2 fragment conjugated to Alexa Fluor® 488 (4412S; 1:500; Cell Signaling Technology, USA) for 2 hours at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... pEGFR (Y1068, #3777) pAKT (S 473, #9271 and p44/42 MAPK (ERK1/2) (T202/Y204, #4370) were purchased from Cell Signaling Technology (Danvers, MA), β- actin was used as a housekeeping proteinand the antibody was bought from EMD Millipore (#MAB1501 now part of Millipore Sigma ...
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bioRxiv - Developmental Biology 2023Quote: ... and then incubated with the following primary antibodies for 2 hours at room temperature in 5% BSA: 1:1000 anti-SMARCC1 (Cell Signaling Technologies cat. #11956T), 1:1000 anti-SMARCA4 (Cell Signaling Technologies cat ...
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bioRxiv - Cell Biology 2023Quote: ... 10% FBS) and incubated with the following primary antibodies in blocking buffer for 2 hours at RT: YAP (Cell Signaling Technology, #14074, 1:100), TAZ (Sigma ...
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bioRxiv - Developmental Biology 2023Quote: ... and the samples were incubated with the following primary antibodies overnight at 4°C: Rabbit anti-p44/42 MAPK (Erk1/2) (1:1000, 4695, Cell Signaling Technology, MA, USA), rabbit anti-phospho-p44/42 MAPK (Erk1/2 ...
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bioRxiv - Cell Biology 2023Quote: ... The NC membrane was incubated for 1 hour at RT in blocking buffer (5% nonfat milk in PBS [pH 7.4] containing 0.1% Triton X-100 and 0.02% NaN3) and immunoblotted for 2 hours at RT with antibodies to CFTR (1:1000, Cell signaling Technology Cat. No. 78335,), SNAP-23 (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... The immunodetection of selected proteins was performed overnight at 4 °C with the following antibodies: p62 (Cell Signaling Technology Inc, Danvers, MA, USA, 5114, 1:1000), LC3b (Abcam ab51520 ...
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bioRxiv - Bioengineering 2023Quote: ... Proteins were transferred onto nitrocellulose membranes for immunoblotting and then probed with antibodies for phospho-AMPKα (Thr172) and β-Actin (Cell Signaling Technology, Danvers, MA, USA). The membrane was probed with appropriate secondary antibodies and imaged on an Odyssey Imaging System (LICOR ...
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bioRxiv - Cell Biology 2023Quote: ... 1 μg/μl (500 μg protein) samples were prepared and incubated overnight at 4 °C in primary antibody against acetyl-lysine (Cell Signaling; Cat No. 9814 (1:100)) ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were incubated in the following primary antibodies for 3 hours at room temperature: phospho-S6 Ribosomal Protein Ser240/244 (rabbit monoclonal, 1:1000 dilution, Cell Signaling Technology, catalog #5364, RRID: AB_10694233), phospho-AKT Ser473 (rabbit monoclonal ...
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bioRxiv - Cell Biology 2019Quote: The following primary and secondary antibodies were used for immunofluorescence: anti-phospho-Myosin Light Chain 2 (Thr18/Ser19) rabbit antibody (no. 3674, Cell Signaling Technology, 1:50 dilution); anti-E-cadherin rabbit antibody (no ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated for 30min in the primary + secondary antibody solution: 2%BSA-PBS + Rabbit anti-mouse Phosopho-Akt (Ser473) antibody (Cell Signaling Technologies 4060; 1:50), Goat anti-Rabbit Alexa 488 secondary antibody (ThermoFisher A32731 ...
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bioRxiv - Microbiology 2022Quote: ... Phosphorylation of MAPKs Slt2 and Fus3 was detected using a rabbit antiPhospho-p44/42 MAPK (Erk1/2) antibody (Thr202/Tyr204; #4370 from Cell Signaling Technology, Danvers, MA, USA). A mouse anti-α-tubulin antibody (#T9026 from Sigma Aldrich ...
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bioRxiv - Immunology 2021Quote: ... blocked with 5% nonfat dry milk for 1 hr and probed with primary Abs: phospho-p44/42 MAPK (ERK1/2) (Thr202/Tyr204, dilution 1:2000, Cell Signaling Technology cat. no. 4370) and alpha tubulin (dilution 1:2000 ...
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bioRxiv - Neuroscience 2019Quote: Immunohistochemical stainings of pERK1/2 and pS6 were performed on 5 μm thick coronal sections of paraffin-embedded mouse brains using primary antibodies raised against pERK1/2 (1:300, Cell Signaling Technology Cat# 4370, RRID:AB_2315112) and pS6 (1:300 ...
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bioRxiv - Cell Biology 2021Quote: ... 2% normal goat serum and 0.2% Triton-X100 in PBS) for 30 minutes and hybridized with polyclonal rabbit anti-phospho-Histone H3 (Ser10) antibody (Cell Signaling Technology, #9701; 1:1000) at overnight at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... pERK1/2 (Thr202/Tyr204, #4370), α-pSTAT5 (Tyr694, #9351), α-Caspase3 (#9662), cleaved Caspase3 (Asp175, #9664), α-pMEK (Ser221, #2338) were purchased from Cell Signaling Technology. For RAF immunoprecipitation ...
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bioRxiv - Cancer Biology 2022Quote: ... and rotated at 4°C overnight with 2-5 ug of antibody (H3K27ac, Active Motif Cat #39133; V5 tag, ThermoFisher #R960-25; ETV6, Santa Cruz, # sc-166835x; ERG, Cell Signaling Technologies # 97249). Subsequent chromatin capture ...
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bioRxiv - Plant Biology 2023Quote: ... MAPK activation was detected by western blot with Phospho-p44/42 MAPK (Erk1/2; Thr-202/Tyr-204) rabbit monoclonal antibodies #9101 (Cell Signaling Technology, Danvers, MA, USA). Blots were stained with PageBlue Protein Staining Solution (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting supernatants (10 μL) were analyzed by Western Blot with Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (Cell Signaling, 1679101S; dilution 1:1,000) primary antibodies ...
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bioRxiv - Immunology 2023Quote: ... Primary antibodies were incubated for 2 h in a solution of TBST (0.05%) and LI-COR blocking buffer (5%) containing the following antibodies: anti-ERK1/2 (Cell Signaling Technologies; dilution factor: 1/2000) and streptavidin IRdye680 (LI-COR Biosciences ...
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bioRxiv - Neuroscience 2021Quote: ... Optical densities were measured by dividing the intensity of the bands by the intensity of the housekeeping protein β-actin (Cell Signaling Technology, MA, USA, 1:1000). Data from control samples were assigned a relative value of 100%.
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bioRxiv - Neuroscience 2020Quote: ... we used identical procedures except that we used LV-Syn-DIO-mTagBFP2-2A-GluN2B (1 × 109 TU/ml titer, 2 μL) and GluN2B antibody (#4207S, Cell Signaling, 1:1000, rabbit polyclonal antibody) (Supplementary Fig ...
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bioRxiv - Cell Biology 2019Quote: ... the membranes were incubated at 37°C for 2 h with the corresponding secondary antibody: horseradish peroxidase (HRP)-linked anti-mouse IgG antibody (#7076, Cell Signaling Technology [CST]; diluted 1:2000) or HRP-linked anti-rabbit IgG antibody (#7074 ...
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bioRxiv - Molecular Biology 2019Quote: ... membranes were then incubated at 4°C overnight in Odyssey Blocking buffer containing 0.1% Tween 20 and the following antibodies: ERK 1/2 (mouse monoclonal, Cell Signaling Technology no. 4696; 1:1000 dilution), pERK 1/2 (rabbit monoclonal ...
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bioRxiv - Immunology 2021Quote: ... anti-MEK1/2 mAb (Clone D1A5, Cat # 8727), and anti-phospho-MEK1/2 mAb (Ser221) (Clone 166F8, Cat # 2338) were purchased from Cell Signaling Technology (Danvers, MA 01923, USA). Human embryonic kidney 293 cells transformed with T antigen of SV40 (HEK293T ...
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bioRxiv - Molecular Biology 2019Quote: ... Primary antibody incubation was carried out overnight in OBB 1:1 in PBS with the anti phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) rabbit monoclonal antibody (#4370 Cell Signaling technology. Used at 1:2000 dilution), the monoclonal anti GFP antibody ((0.4mg/ml) ...
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bioRxiv - Genomics 2023Quote: ... Fixed and permeabilized cells were then blocked in 0.5% BSA and 0.2% Tween-20 in PBS (IF blocking buffer) for 30 minutes at room temperature and incubated with anti-Ngn2 (Cell Signaling Technology, cat# 13144; 1:250 dilution) and anti-MAP2 (Biolegend ...
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bioRxiv - Cancer Biology 2022Quote: ... and probed overnight at 4°C with the following primary antibodies diluted in 5% BSA in TBST: pMEK1/2 (Cell Signaling Technology, Danvers, MA, USA, 41G9, 1:1000), pERK1/2 (Cell Signaling Technology ...
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bioRxiv - Microbiology 2020Quote: Cells were lysed in cell lysis buffer (Cell Signaling) containing protease inhibitor cocktail (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2021Quote: ... Five-micron paraffin-embedded sections were labeled with primary antibodies against phosphor-p44/p42 MAPK (Erk1/2) (Thr 202/Tyr 204) (Cell Signaling Technology Cat# 4370, 1:200, Danvers, MA, USA), Ki-67 (ab15580 ...
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bioRxiv - Cancer Biology 2021Quote: Cells were lysed in cell lysis buffer (Cell Signaling Technology) with added protease and phosphatase inhibitor cocktails (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2021Quote: ... cells were lysed with 1x cell lysis buffer (Cell signaling). For mouse tissues ...
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bioRxiv - Physiology 2021Quote: ... Cells were lysed using cell lysis buffer (Cell Signaling, 9803S). All samples were centrifuged at 22000xg for 10 minutes at 4C and transferred to fresh tubes to remove insoluble debris ...
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bioRxiv - Immunology 2022Quote: Cell lysates were prepared using cell lysis buffer (Cell Signaling), incubated on ice for 60 min and spun at 4°C at 13K to remove insoluble material ...
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bioRxiv - Systems Biology 2020Quote: ... Cells were lysed in cell lysis buffer (Cell Signaling Technology) containing 1 mM PMSF and analyzed by SDS-PAGE and western blotting using anti-EB1 (BD Bioscience ...
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bioRxiv - Bioengineering 2021Quote: ... These cells were lysed with cell lysis buffer (Cell signaling) containing 1 mM PMSF ...
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bioRxiv - Molecular Biology 2020Quote: Transfected cells were lysed in cell lysis buffer (Cell signaling) with 1x protease inhibitor cocktail (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... cells were lysed in cell lysis buffer (Cell Signaling Technology) and centrifuged for 10 min at 15,000 x g at 4°C ...
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bioRxiv - Immunology 2022Quote: ... cells were lysed with cell lysis buffer (Cell Signaling Technology) with protease inhibitor cocktail (Complete ...
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bioRxiv - Neuroscience 2023Quote: ... cells were suspended in Cell Lysis Buffer (Cell Signaling Technology) supplemented with 10µM PMSF and 1x protease/phosphatase cocktail (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were lysed using 10X Cell Lysis Buffer (Cell Signaling) supplemented with Complete Mini protease inhibitor cocktail and PhosSTOP phosphatase inhibitors (Roche) ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were lysed using Cell Lysis Buffer (Cell Signaling, 9803). Protein concentrations were determined using a BCA assay (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2020Quote: Cells were lysed in Cell Signaling Lysis Buffer (Cell Signaling Technology) as previously reported13 and resolved by gel electrophoresis using Novex 4-12% Bis-Tris Gels (Invitrogen) ...
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bioRxiv - Cancer Biology 2022Quote: Cells were lysed using 10X Cell Lysis Buffer (Cell Signaling Technologies), supplemented with EDTA-free protease inhibitor cocktail tablets (Roche) ...
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bioRxiv - Microbiology 2020Quote: ... Cells were harvested and lysed in cell lysis buffer (Cell Signaling) supplemented with protease inhibitor cocktails (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were lysed with the Cell Lysis Buffer (Cell Signaling, 9803S). Subsequentially ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then lysed in 10x Cell Lysis Buffer (Cell Signaling) supplemented with EDTA-free protease inhibitor (Roche ...
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bioRxiv - Neuroscience 2020Quote: Cells were lysed with cell lysis buffer (Cell Signaling Technologies, #9803) supplemented with protease inhibitor cocktail (Roche ...
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bioRxiv - Immunology 2022Quote: ... cells were lysed with Cell Lysis Buffer (Cell Signaling Technology, 9803) and whole-cell lysates (WCL ...