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Citations for Cell Signaling Technology :
1551 - 1600 of 10000+ citations for Dengue Virus Serotype 2 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2022Quote: ... We quantified changes in protein levels relative to control using Image Lab software after normalization to β-actin or GAPDH (Cell Signaling Technologies), as indicated ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies to the following proteins were used at the indicated concentrations: Ubq (1:5000, P4D1, Cell Signaling; 1:5000, P4G7-HRP, BioLegend), Actin (1:10,000 ...
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bioRxiv - Biophysics 2023Quote: ... mAb #3288) and Lysosome Associated Membrane Protein 1 (LAMP1) specific rabbit monoclonal antibody (cat. no. mAb #9091) were purchased from Cell Signaling Technology (USA). Anti-caveolin-1 rabbit polyclonal antibody was acquired from BD Biosciences (USA ...
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bioRxiv - Cancer Biology 2023Quote: ... Each blot was then washed thrice in 1X TBST for 5 minutes and proteins were detected using either LumiGLO/Peroxide Reagent or SignalFire™ ECL reagent (7003 and 6883, respectively, Cell Signaling Technology) and an iBright™ imaging system (ThermoFisher) ...
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bioRxiv - Plant Biology 2020Quote: ... membranes were incubated with the antibody against Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® (Cell Signaling Technology, USA). As a loading control membranes were incubated with anti-tubulin antibody (Abiocode ...
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bioRxiv - Cancer Biology 2020Quote: ... for 1 hour and probed overnight at 4°C with the primary antibodies of interest directed against: rabbit anti-BCL-2 (Cell Signaling, CST4223), rabbit anti-BCL-xL (Cell Signaling ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were blocked in 5% milk and incubated in 2% BSA with primary antibodies to GAPDH (Cell Signaling Technology Cat# 13084, RRID:AB_2713924), Eg5 (Sigma Aldrich Cat# SAB4501650 ...
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bioRxiv - Developmental Biology 2020Quote: ... Cells were permeabilized with 0.1% Triton-X and stained for intracellular phospho-p44/42 Erk1/2 Thr202/Tyr204 at 1:700 dilution (Cell Signaling Technology, 4370) and p44/42 Erk1/2 at 1:700 dilution (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2022Quote: ... and the pellet containing the DOC-insoluble material was resuspended in 100 μl SDS solubilization buffer, pH8.8 (20 mM Tris-Cl, 2 mM EDTA, 1% w/v SDS, 1X protease inhibitor cocktail (Cell Signaling Technology, 5871). The DOC-insoluble pellet was thoroughly dissolved by heating the sample to 95 °C and vortexing ...
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bioRxiv - Neuroscience 2021Quote: ... Then the cells were blocked in 10% donkey serum for 2 h and incubated with primary antibodies (goat anti-Iba1, 1:400, Abcam; mouse anti-CD68, 1:400, Cell Signaling Technology; rabbit anti-Arg-1 ...
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bioRxiv - Developmental Biology 2020Quote: ... Nuclei pellets were washed twice with 200 μL of Dig-wash buffer before resuspending in 100 μL Dig-wash buffer supplemented with 2 mM EDTA and a 1:50 dilution of H3K27ac primary antibody (Cell Signaling, 8173) and incubated overnight at 4°C on an overhead rotator ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-ERK1/2 (dilution 1:2000) and rabbit α-tubulin (dilution 1:2000) all purchased from Cell Signaling Technology (Danvers, MA). The phospho-ERK1/2 expression was evaluated first using a 680-nm secondary anti-mouse antibody ...
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bioRxiv - Cancer Biology 2020Quote: ... discs were washed 5 times for a total of 30 minutes with 0.1% PBST and incubated overnight in goat anti-mouse F(ab)’2 AlexaFluor-555 secondary antibody (Cell Signaling, 1:500). Finally ...
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bioRxiv - Plant Biology 2020Quote: ... Phosphorylated MAPKs were detected using α-phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) rabbit monoclonal antibody (1:2000, Cell Signaling Technology, USA) for 1 h at room temperature in a solution of 5 % (w/v ...
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bioRxiv - Microbiology 2021Quote: ... Anti-SARS-CoV-2 spike antibody (BEI Resources NR-52947) was used at 1:1000 and anti-rabbit HRP (Cell Signaling Technology) at 1:1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... and incubation with the following primary antibodies: phospho-p44/42 MAPK (EER1/2) (T202/Y204) (Cell Signaling Technology, Cat. #: 9101, 1:1000), p44/42 MAPK (ERK1/2 ...
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bioRxiv - Cell Biology 2019Quote: ... The parasites were incubated for 2 hours at room temperature with the primary antibodies 1:1000 anti-HA (C29F4) Rabbit monoclonal antibody (mAb) from Cell Signaling Technology® ...
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bioRxiv - Systems Biology 2019Quote: ... Membranes were probed with rabbit monoclonal antibodies directed against the diphosphorylated (Thr202/Tyr204) form of ERK1/2 (p42/44 MAPK, Cat. No. 4372, Cell Signaling Technology) and total ERK1/2 (Cat ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were then incubated with appropriate secondary antibodies for 1h: Alexa Fluor 488 or 555 or 647 Goat anti-Mouse IgG (2 μg/mL, Cell Signaling Technology). Cells were then permeabilized as previously described (Souriant et al. ...
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bioRxiv - Cell Biology 2019Quote: ... Samples were then incubated overnight on a plate rocker at 4° C in a 1:200 dilution of anti-phospho-Erk1/2 (Thr202/Tyr204) rabbit monoclonal antibody (Cell Signaling 4370) in blocking buffer ...
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bioRxiv - Cell Biology 2021Quote: ... rabbit anti-human myosin light chain 2 (D18E2) [with cross-reactivity to mouse] (catalog # 8505, RRID:AB_2728760; Cell Signaling Technology, Danvers, MA, USA), and goat anti-human GAPDH [with cross-reactivity to mouse] (catalog # AF5718 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Vero E6 hTMPRSS2 cells infected with SARS-Cov-2 were lysed and analyzed by Western blot as done in with Rabbit Anti-SARS CoV-2 (COVID-19) Spike (Cat No. GTX135356) (Gene Tex) and Rabbit anti-Vinculin #13901 (E1E9V XP®) (Cell signaling) as loading control ...
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bioRxiv - Plant Biology 2020Quote: ... or phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (anti-phospho-TEY, #9101, 1:200, Cell Signaling Technology, Danvers, MA, USA). Membranes were washed 3 times for 5 min with TBST and incubated for 1 h with the appropriate secondary antibody – goat anti-rabbit (1:20,000 ...
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bioRxiv - Cancer Biology 2021Quote: ... MAPK activity was assessed using phospho-p44/42 MAPK (ERK1/2) (Thr202/Tyr204) primary antibody (1:200, rabbit, Cell Signaling Technologies #9101), total p44/42 MAPK (ERK1/2 ...
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bioRxiv - Pathology 2021Quote: ... The separated proteins were transferred onto a nitrocellulose membrane for detection with polyclonal rabbit anti-Phospho-Myosin Light Chain 2 (Ser19) antibody (Cell Signaling Technologies) or monoclonal rabbit anti-Myosin Light Chain 2 (D18E2 ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were spun down and washed three times with cold Flow Wash Buffer (FWB) (PBS, 2% FBS, 2mM EDTA) and incubated with primary antibody (EGFR-D1D4J, Cell Signaling Technology) diluted in FWB for 60 minutes on ice ...
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bioRxiv - Microbiology 2022Quote: ... cells were blocked with 5% milk in PBS for 1 h and then incubated with α-SARS-CoV-2 nucleocapsid antibody (1:3,000; #26369 Cell Signaling Technology) overnight at 4°C ...
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bioRxiv - Plant Biology 2022Quote: ... Protein electrophoresis and Western-Blot were performed as described previously using the Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (Cell Signaling Technology) (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... was done overnight at 4°C and was followed by incubation with anti-mouse IgG (H+L) F(ab’)2 conjugated to Alexa Fluor 647 (Cell Signaling Technology). Images were acquired with ZEISS LSM 980 laser-scanning confocal microscope and analysed using ZEN software (Carl Zeiss MicroImaging).
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bioRxiv - Cell Biology 2023Quote: ... anti-PKM 1/2 (C103A3, #3190), anti-PKM2 (D78A4, #4053), and anti-pY849 PDGFRα/pY857PDGFRβ (C43E9, #3170S) antibodies were purchased from Cell Signaling Technology. Rabbit monoclonal anti-GLUT1 (ab115730) ...
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bioRxiv - Immunology 2023Quote: ... Cells were then washed twice in 1 ml FACS buffer and resuspended in 50-100 μl of 1:50 diluted primary PE-conjugated antibodies against phosphorylated p38 or Erk1/2 (Cell Signaling Technology) and stained for 1 h at room temperature on a rotor ...
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bioRxiv - Immunology 2023Quote: ... Primary antibodies to EGR1, EGR3, phospho-ERK1/2, GAPDH and actin, and an inhibitor of EphA2 (dasatinib, 100 nM) were from Cell Signaling Technologies. Secondary HRP-conjugated secondary antibodies used were goat anti-rabbit (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2023Quote: ... These steps were followed by the incubation with primary antibodies overnight at 4°C in a solution of 2% NGS in PBS 1x: chicken anti-mCherry (1:1000, HenBiotech, Portugal) and rabbit anti-NeuN (1:100, Cell Signaling, USA), rabbit anti-Iba1 (1:750 ...
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bioRxiv - Developmental Biology 2023Quote: ... The embryos were incubated in rabbit anti-phospho-Myosin Light Chain 2 (Thr18/Ser19) antibody (1:200, catalog # 3678, Cell Signaling Technology) in 1% BSA/PBST overnight at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... Primary antibody incubation was performed for 1h at room temperature in phospho-p44/42 MAPK (ERK1/2) (Thr202/Thr304) antibody (#4370, 1:200, Cell Signaling Technology) or human VE-Cadherin antibody (#AF938 ...
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bioRxiv - Molecular Biology 2024Quote: ... and incubated overnight at 4°C with primary antibody detecting light chain 3 type 1 and 2 (Cell Signaling technology, catalog #3868), SOAT1 (catalog #84476) ...
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bioRxiv - Cancer Biology 2024Quote: ... anti-phospho-p44/42 MAPK T202/Y204 (p-ERK 1/2, #9101, 1:1000), anti-PARP (#9532, 1:1000), and anti-LAMP1 (#9091, 1:400) from Cell Signaling Technologies and anti-Myc-Cy3 (Sigma) ...
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bioRxiv - Immunology 2024Quote: ... Cells were incubated rotating for 2 hr at 4°C with 1 uL anti-tri-methyl-histone H3 (Lys27) (C36B11, Cell Signaling Technology) or normal rabbit IgG (#2729 ...
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bioRxiv - Pathology 2023Quote: ... formalin fixed paraffin embedded tissue sections were stained with rabbit-anti claudin-2 (1:100, Cell Signaling Technology Cat# 28530, RRID: AB_2798961), and rat-anti CD45 (1:100 ...
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bioRxiv - Genetics 2021Quote: ... and probed for phosphorylated EIF2α protein levels with rabbit anti-Eif2a S51 phosphorylation polyclonal antibody (1:500 dilution) (Cell Signaling Technologies, cat. number D9G8) and Total EIF2α protein levels with rabbit anti-EIF2a polyclonal antibody (1:10,000 dilution ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibodies to all native and/or phosphorylated proteins (see Supplementary Table S1 for details) except for WT1 were purchased from Cell Signaling Technology (Boston, MA). The C-terminal anti-WT1 antibody (C-19 ...
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bioRxiv - Cancer Biology 2021Quote: ... Western blot was performed as described before and the following antibodies were used for protein detection: β-tubulin (Cell Signaling Technology, β-Tubulin (9F3) Rabbit mAb #2128 ...
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bioRxiv - Cancer Biology 2021Quote: ... A fraction of each sample was then precleared with protein A and salmon sperm DNA-bound agarose beads (Cell Signaling Technologies, Cat. No 9863), for 1 hour in 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... A fraction of each sample was then precleared with protein A and salmon sperm DNA-bound agarose beads (Cell Signaling Technologies, Cat. No 9863), for 1 hour in 4°C ...
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bioRxiv - Microbiology 2020Quote: ... of the sample was incubated with 3 μg of antibody (or 3 μg of IgG as control) and 30 μl of protein G magnetic beads (Cell Signaling Technology, Cat. 7008) at 4 °C overnight ...
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bioRxiv - Cancer Biology 2021Quote: ... A fraction of each sample was then precleared by incubation with protein A and salmon sperm DNA-bound agarose beads (Cell Signaling, Cat. No 9863), for 1 hour in 4oC ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated for 48 hr at 4°C with antibodies against phosphorylated S6 ribosomal protein (Ser240/244, 1:800, Cell Signaling Technology, catalog # 5364S), GFP (1:1000 ...
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bioRxiv - Molecular Biology 2019Quote: ... Tween and detection of total CHK1 or CHK2 proteins was carried out for 1 h at RT using mouse-anti-Chk1 mAb (2G1D5) (Cell Signaling 2360; Lot 3) or mouse anti-Chk2 mouse mAb (BD Biosciences 611570) ...
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bioRxiv - Neuroscience 2019Quote: ... anti-Microtubule-associated proteins 1A/1B light chain 3B (LC3B-I/-II) at 1:1000 dilution (14, 16 kDa, Cell Signaling Technology, Danvers, MA); anti-monocarboxylate transporter 1 (MCT1 ...
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bioRxiv - Cancer Biology 2019Quote: ... In order to positively identify the proteins, the following antibodies were used: MAN1B1 (SC-100543, 1:500) and actin (1:2000) (Cell Signaling Technology, Beverly, MA). Horse radish peroxidase (HRP)-conjugated secondary antibodies were incubated with the membrane at RT for 1 hr ...