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Citations for Cell Signaling Technology :
1601 - 1650 of 10000+ citations for Dengue Virus Serotype 2 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... The following primary antibodies were used for protein immunoblots: Anti p-Tyr (4G10, Millipore Sigma, #05-321 and P-Tyr-1000, Cell Signaling Technology, #8954), anti V5-HRP (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were separated on a 4–12% SDS-PAGE gel followed by standard immunoblot analysis using anti–CISH (Cell Signaling, Clone D4C10, 1:2000) and Vinculin (Cell Signaling ...
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bioRxiv - Cancer Biology 2022Quote: ... we validated the presence of the correctly sized tagged MYC protein and absence of wild type MYC by Western blot probed with a c-MYC antibody (Rabbit mAb, Cell Signaling Technology, Cat# 5605). We selected homozygous clone number 2 (for N-BioTAP-cMYC ...
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bioRxiv - Neuroscience 2022Quote: ... Anti-ionized calcium-binding adaptor molecule 1 (Iba-1, FUJIFILM Wako Chemicals USA, Richmond, VA) and anti-glial fibrillary acidic protein (GFAP, Cell Signaling Technology, Danvers, MA) antibodies were diluted to 1:1,000 and used to incubate tissue sections overnight at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... The expression levels of target proteins were assessed by western blotting (Figure S1) with antibodies against ADA (clone D1P4Y, Cell Signaling Technology, Cat#65184), ADSSL1 (clone 2D12 ...
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bioRxiv - Plant Biology 2023Quote: ... The bead-bound proteins were separated by SDS-PAGE and detected by western blotting with the MBP antibody (Cell Signaling Technology, Danvers, MA, USA), His-tag antibody (Tiangen ...
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bioRxiv - Immunology 2023Quote: ... Abcam Cat# ab270521).The following antibody and the concentration used against human proteins were employed: αEGFR (1:1000 for western blot; Cell Signaling Technology Cat# 4267, RRID:AB_2246311), αEGFRL858R mutant specific (1:100 microscopy ...
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bioRxiv - Genomics 2023Quote: ... The presence of the correctly tagged MYC protein was validated by Western blot probed with a c-MYC antibody (Rabbit mAb, Cell Signaling Technology, Cat# 5605). The recombinant pAAV-CRE plasmid was packaged in HEK293T cells using pHelper and pRC2-mi342 plasmids (Takara ...
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bioRxiv - Cancer Biology 2024Quote: Total protein was extracted from spheroids and organoids using lysis buffer supplemented with a protease/phosphatase inhibitor cocktail (both from Cell Signaling Technology, MA, USA). Protein concentrations were measured using a Nanodrop 2000 system (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... Lysates were then precleared by incubation for 1 hour at 4°C while rotating with 50 μL protein A agarose beads (Cell Signaling Technologies, Cat #9863) CCNT1 antibody (Cell Signaling Technologies ...
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The E3 ubiquitin-protein ligase MDM2 is a novel interactor of the von Hippel-Lindau tumor suppressorbioRxiv - Biochemistry 2020Quote: ... Co-IPs of endogenous proteins was performed using 500 μgr of PNS incubated with 2 μg of anti-MDM2 or 1 μg of anti-VHL (Cell Signaling technology,68547). The following antibodies were used Anti-GFP ab1218 Abcam (Ip) ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibodies for Western Blot [Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody #9101] were procured from Cell Signaling Technology (Danvers, MA, USA). Antibodies for flow cytometry were procured from BioLegend and eBioscience (San Diego ...
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bioRxiv - Synthetic Biology 2021Quote: ... Membranes were then incubated overnight at 4°C with rabbit anti-phosphorylated ERK1/2 antibody (Cell Signaling Technology; 9101S; LOT: 30; 1:1,000) in TBST containing 5% (w/v ...
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bioRxiv - Microbiology 2020Quote: ... MAP kinase activation was detected by western-blot analysis with anti-phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (Cell Signaling Technology, 9101S) and anti-rabbit horseradish peroxidase-linked sheep antibody ...
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bioRxiv - Cell Biology 2022Quote: ... cells were collected by centrifugation at 500 × g for 5 minutes and resuspended in 1 ml TNE buffer (150 mM NaCl, 2 mM EDTA, 50 mM Tris-HCl, pH 7.4) containing protease and phosphatase inhibitors (Cell Signaling Technology, Danvers, MA), passed through a 25 gauge needle to shear the cells ...
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bioRxiv - Biochemistry 2021Quote: ... Anti-mouse IgG (H+L) and F(ab’)2 Fragment Alexa Fluor® 555-conjugate (#4409) were purchased from Cell Signaling Technologies (CST). Mouse monoclonal (M2 ...
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bioRxiv - Physiology 2019Quote: ... phospho-p44/42 MAPK Thr202/Tyr204 antibody (rabbit, 1:2000) and Erk1/2 antibody (rabbit, 1:2000; all from Cell Signaling Technology, USA), and a-tubulin antibody (mouse,1:1000 ...
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bioRxiv - Cancer Biology 2019Quote: ... then stained with Hoechst 33342 (2 µg/mL) in OBB and antibodies against p-ERK (1:200, Cell Signaling Technologies, Cat. No. 4370S), then anti-rabbit IgG (1:2000 ...
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bioRxiv - Physiology 2021Quote: ... anti-phospho and total extracellular signal-regulated kinase antibody (p/total ERK 1-2 1:1000 CST4396, CST4696, Cell Signaling Technology, Danvers, MA), anti-phospho and total protein kinase B antibody (AKT 1:1000 ...
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bioRxiv - Plant Biology 2022Quote: ... was analyzed for MAPK phosphorylation by immunoblotting using the primary antibody anti-pERK MAPK (Phospho-p44/p42 MAPK, ERK1/2,Thr202/Tyr204, D13.14.4E; Cell Signaling Technology, Danvers, MA, USA) at a dilution of 1:2,500 and the secondary antibody (Goat anti-rabbit IgG (H+L)-HRP conjugate ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated at room temperature for 2 hours or overnight at 4°C with the following primary antibodies: Sirt3 (1:200, Cell Signaling Technology 5490S), TOMM20 (1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... phospho-ERK1/2 (D13.14.4E; Cat. #4370) and mouse antibodies again ERK1/2 (L34F12; Cat. #4696) and β-Actin (8H10D10; Cat. #3700) are from Cell Signaling Technology. Rabbit anti-Cytokeratin 10 (Cat ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were blocked in 2% milk-phosphate-buffered saline (PBS) and incubated with the following antihuman antibodies: rabbit β3-integrin (1:1000; 13166, Cell Signaling Technology [CST]), rabbit p-FAK (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were blocked with TBS containing 2% (m/v) bovine serum albumin (BSA) and then incubated with antibodies raised against the HA epitope (Cell Signaling Technology, USA) diluted 1:1000 in the same solution ...
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bioRxiv - Cell Biology 2024Quote: ... All cell pellets were then resuspended in 100 µL fresh MG medium and stained with 1.4 µM 4’,6-diamidino-2-phenylindole (DAPI) from Cell Signaling Technology (Cat #4083S) for 15 min in the dark at 30 °C in the microaerobic chamber ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated with anti-rabbit IgG (H + L) F (ab′)2 fragment (Alexa Fluor® 488 conjugate) (4412s, 1:200, Cell Signaling Technology) for 3 h at 25°C ...
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bioRxiv - Immunology 2023Quote: ... and equal volume of 2X STOP Buffer (Buffer 2 + 20uM EDTA + 4uM EGTA) was added along with 1 pg of Saccharomyces cerevisiae spike-in DNA (Cell Signaling, cat. 29987). Samples were incubated for 15 mins at 37°C and DNA was isolated and purified using Qiagen MinElute Kit according to manufacturer’s protocol and subjected to library amplification50,51.
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bioRxiv - Cell Biology 2023Quote: ... Rabbit anti phospho-myosin light chain 2 Thr18/Ser19 (pMLC, 1:50 for IF, 1:20 for IG) was from Cell Signaling Technologies (#3674). Chicken anti-map2 (against residues 2-314 of human map2 ...
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bioRxiv - Molecular Biology 2021Quote: ... The same membrane that was used to detect AIRE protein was washes and reused in an incubation with an anti-GAPDH rabbit polyclonal primary antibody (Cell Signaling Technology, Beverly, MA, USA) following incubation with peroxidase conjugated anti-rabbit antibody.
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bioRxiv - Cancer Biology 2020Quote: ... Protein amounts of OP18 and beta-actin were quantified using a primary stathmin polyclonal antibody (1:1000; Cell Signaling Technology, NEB GmbH, Frankfurt/Main, Germany) and a polyclonal beta-actin antibody (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were incubated with primary antibody in 10 % Donkey Serum in PBS overnight at 4 °C: Phospho-S6 Ribosomal Protein (Ser240/244) Rabbit mAb (Cell Signaling Technology, Cat# 5364; RRID:AB_10694233). After HSCs were washed three times with PBS + 0.1 % Tween-20 ...
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bioRxiv - Cell Biology 2021Quote: ... Forty-five μg of protein were resolved in SDS-PAGE and blotted with anti-phospho-eNOS (Ser1177) antibodies (Cell Signaling, Danvers, MA, USA, 1:250), followed by incubation with goat anti-rabbit horseradish peroxidase secondary antibodies (Jackson ImmunoResearch ...
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bioRxiv - Plant Biology 2022Quote: ... The extracts were then incubated at 28°C and the samples were collected at the indicated intervals for determination of GST and GST-WL1 protein abundance by western blots using the anti-GST (#2625, Cell Signaling Technology, 1:1000 dilution) and alkaline phosphatase-conjugated anti-rabbit (# 31340 ...
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bioRxiv - Developmental Biology 2021Quote: ... 20 μg of protein per lane were subjected to SDS-PAGE and probed with the following primary antibodies: YAP (Cell Signaling Technologies #8418, 1:500), YAP/TAZ (Cell Signaling #4912 ...
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bioRxiv - Neuroscience 2020Quote: ... Astrocytes were then collected by scraping and the CNMCS Compartmental Protein Extraction Kit (BioChain Cat# K3013010 Hayward, CA) with protease/phosphatase inhibitors (PI, Cell Signaling, Cat# 5872S, Danvers, MA) was used and the nuclear fractions were isolated for each treatment condition ...
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bioRxiv - Immunology 2020Quote: ... Antibodies used to detect proteins involved in glycolysis (Hk1 clone C35C4, Glut1 clone D3J3A and HIF-1a D1S7W) were from Cell Signaling (all at 1:500) were used to intracellular staining ...
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bioRxiv - Systems Biology 2022Quote: ... Immunoblot analysis was used to assess the adequacy of nuclear purification by measuring Lamin A/C (rabbit, 1:1000, Cell signaling # 2032, a nuclear protein).
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bioRxiv - Physiology 2023Quote: ... we loaded 25 µg protein of each sample to SDS-PAGE gels and incubated the membranes with 1:1000 eIF2α antibody (Cell Signaling Technology #9722, Danvers, MA) or 1:1000 Phospho-eIF2α antibody (Cell Signaling Technology #9721 ...
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bioRxiv - Neuroscience 2023Quote: ... which is consistent with other validation western blots published by vendors for this protein as well as manuscripts listed above (Cell Signaling Technologies, Novus, Rockland, Proteintech). The anti-SARS-CoV-2 spike protein antibody (GTX632604 ...
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bioRxiv - Neuroscience 2023Quote: Primary antibodies to the following proteins were used at the indicated concentrations: ubiquitin (1:1000, P4G7, BioLegend; 1:200 Cell Signaling; 1:200, Z0458, Dako), GABARAP (1:1,000 ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein lysates were extracted at 3 hours post-treatment and analysed by western blot following overnight incubation with antibodies against the following primary proteins: Vinculin (E1E9V, rabbit, Cell Signaling Technology, 13901, 1:1000), Phosphorylated p44/42 MAPK (ERK1/2 ...
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bioRxiv - Cell Biology 2021Quote: ... The antibodies used included the following: rabbit anti-TCF7L2 (1:1,000 for western blotting and 2 μg for co-IP assays; #2569; Cell Signaling Technology, Danvers, MA, USA), rabbit anti-TCF7 (1:1,000 for western blotting ...
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bioRxiv - Biochemistry 2021Quote: ... or the H2.3 rabbit polyclonal anti-HSulf-2 antibody (dil. 1/500) followed by a horseradish peroxidase-labeled anti-mouse or rabbit IgG antibody (Cell Signaling Technology, Danvers, MA) and ImmunoStar LD (Wako Pure Chemical Industries) ...
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bioRxiv - Microbiology 2020Quote: ... blocked with 5% skimmed milk powder at room temperature for 1 h, the membrane was incubated with the target primary antibody with desired dilution (anti-ACE-2, 1:1000 (Cell signaling technology, MA, USA); TMPRSS2 ...
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bioRxiv - Synthetic Biology 2021Quote: ... membranes were probed with the following antibodies at 1/2500 or 1/5000 dilution: rabbit anti-p44/42 MAPK (ERK1/2) antibody (Cell Signaling, cat # 4695 S) to detect ERK2 ...
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bioRxiv - Molecular Biology 2021Quote: ... Anti-phospho-SAPK/JNK antibody (#9251) and anti-phospho-p44/42 MAPK (Erk1/2) antibody (#9101) were obtained from Cell Signaling Technology (MA, USA). The PPARγ inhibitor ...
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bioRxiv - Biochemistry 2021Quote: Cleaved-caspase-3 immunostaining was performed on 2 µm thick formalin-fixed paraffin sections in a 1:200 dilution according to the manufacture’s protocol (Cell Signaling Technology, Danvers, MA, USA). Pictures were taken on a slide scanner SCN4000 in a 20× magnification.
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bioRxiv - Cell Biology 2022Quote: ... Blots were washed for 15 min in TBST four times then incubated for 2 hours at room temperature with an anti-rabbit IgG HRP-linked secondary antibody (Cell Signaling #7074; 1:20,000). Blots were imaged and quantified using ImageQuant (Amersham).
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bioRxiv - Microbiology 2022Quote: ... Phosphorylation of Mpk1 and Fmk1 MAPKs was detected using rabbit anti-Phospho-p44/42 MAPK (Erk1/2) antibody (Thr202/Tyr204, #4370; Cell Signaling Technology, Danvers, MA). Phosphorylation of Hog1 MAPK was detected using rabbit anti-Phospho-p38 MAPK antibody ((Thr180/Tyr182 ...
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bioRxiv - Cell Biology 2023Quote: ... hypoxia inducible factor 1 subunit alpha (HIF1A; 1:1000, no. #14179), Bax (1:1000, no. #2772), and Bcl-2 (1:1000, no. #4223) (Cell Signaling Technology, Inc., USA). Following primary antibody incubation ...