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Citations for Cell Signaling Technology :
1201 - 1250 of 1318 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Detection of pSTAT5 was performed using a PathScan® Phospho-Stat5 (pTyr694) Sandwich ELISA Kit (Cell Signaling Technology Cat# 7113). Detection of pSTAT6 was performed using a PathScan® Phospho-Stat6 (pTyr641 ...
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bioRxiv - Cancer Biology 2023Quote: Samples for ChIP-seq experiments were prepared using the SimpleChIP Plus Enzymatic chromatin IP kit (Cell Signaling Technology, Cat. 9005) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... Immunoprecipitation was performed using a PAR-CLIP kit (Axl-Bio, China) and anti-HuR antibody (Cell Signaling Technology, USA, 12582S) according to the instructions ...
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bioRxiv - Neuroscience 2024Quote: ChIP-seq analysis from P35-42 mouse cortex was performed using SimpleChIP Enzymatic Chromatin IP Kit (Cell Signaling Technology, #9003) following manufacturer’s instructions with slight modifications ...
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bioRxiv - Biochemistry 2023Quote: Kε-GG peptides were enriched using the PTMScan HS Ubiquitin/SUMO Remnant Motif (Kε-GG) Kit from Cell Signaling Technologies using the provided reagents and protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... samples were subjected to enrichment of tyrosine phosphorylated proteins using an the PTMScan HS phopshotyrosine kit (Cell Signaling Technology, #38572) following in-solution tryptic digestion and desalting ...
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bioRxiv - Cancer Biology 2022Quote: Senescence was assessed using the Senescence β-Galactosidase Staining Kit at pH 6 following the manufacturer’s instructions (Cell Signaling Technology). For SAHF staining ...
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bioRxiv - Molecular Biology 2023Quote: ... Senescence-associated (SA) β-galactosidase activity was determined by using the Senescence SA-β-Galactosidase Staining Kit (Cell Signaling, #9860), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... DiGly peptide enrichment was performed using the PTMScan® Ubiquitin Remnant Motif (K-ε-GG) Kit (Cell Signaling Technology (CST)) ...
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bioRxiv - Cancer Biology 2023Quote: Senescence-associated β-galactosidase staining was performed using a senescence β-galactosidase staining kit (Cell Signaling Technology, cat. no. 9860) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2024Quote: ... was used as secondary for 30 min and the signal was developed using the SignalStain DAB Substrate Kit (Cell Signaling). For IF ...
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bioRxiv - Neuroscience 2024Quote: β-Gal staining was performed with a Senescence β-Galactosidase Staining Kit (Cat. number 9860; Cell Signaling Technology, Danvers, MA, USA), following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... and incubated with annexin V and propidium iodide (PI) as per the manufacturer’s instructions (Early Apoptosis Detection Kit, Cell Signaling Technology). Cells were analyzed by flow cytometry using a BD FACSCanto cell analyzer (BD Biosciences) ...
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bioRxiv - Physiology 2022Quote: The acetyl peptide enrichment was performed on 3 mg of digested peptides using PTMscan Acetyl-Lysine Motif kit (Cell Signaling #13416). The peptides were mixed with 100 µL of 10x IP buffer (500 mM MOPS ...
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bioRxiv - Molecular Biology 2021Quote: ... SA-β-gal activity staining was performed at pH 6.0 (Kurz et al., 2000) using a commercial kit (Cell Signaling, #9860). To compare the intensity of SA-β-gal staining ...
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bioRxiv - Biochemistry 2020Quote: ... and measured IR phosphorylation by using the PathScan® Phospho-Insulin Receptor β (panTyr) Sandwich ELISA Kit (Cell Signaling Technology; #7082).
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bioRxiv - Cancer Biology 2020Quote: ... Cells were lysed on ice at 1000 cells/µL lysis buffer according to the manufacturer’s protocol of the active RAS detection kit (Cat.#8821; Cell Signaling Technology). Total protein concentrations of samples were measured using a BCA protein-assay kit (Cat.#23225 ...
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bioRxiv - Systems Biology 2020Quote: ... DiGly remnant containing peptides were enriched using the PTMScan® Ubiquitin Remnant Motif (K-ɛ-GG) Kit (Cell Signaling Technology (CST)) ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins samples were processed in SDS free lysis buffer for the assessment of Ras-GTP level using Active Ras Detection Kit (Cell Signaling) by following manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... Senescence-Associated β-galactosidase activity was assessed using a SA-β-galactosidase staining kit according to the manufacturer’s instructions (Cell Signaling). Positive cells were counted manually out of more than 100 cells total.
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bioRxiv - Cancer Biology 2021Quote: ... Detection of the HRP/ peroxidase enzyme was performed with SignalStain® DAB Substrate Kit from Cell Signaling (cat. no. 8059; www.cellsignal.com), per the manufacturer’s instructions.
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bioRxiv - Genomics 2022Quote: ... Cleaved Caspase1 and cleaved Gasdermin D antibodies were purchased as a part of Pyroptosis Antibody Sampler Kit (#43811T, Cell Signaling Technology). After overnight primary incubation ...
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bioRxiv - Cancer Biology 2020Quote: ... Ubiquitinated peptides were enriched by immunoaffinity purification using the PTMScan Ubiquitin Remnant Motif (K-ε-GG) Kit (Cell Signaling Technology, 5562) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... ChIP was performed using an amount of BEL-ChIP nuclear extract corresponding to 3.4 μg of input genomic DNA in 250 μL of ChIP Buffer + 1X PIC (SimpleChIP Kit #9003, Cell Signaling Technology). Antibody was added according to the vendor’s specifications and incubated overnight with end-over-end mixing at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... All ChIP was performed using the SimpleChIP Enzymatic Chromatin IP kit with magnetic beads according to the manufacturer’s instructions (Cell Signaling Technologies). DNA levels were quantified by qPCR amplification using Power SYBR Green Mastermix (Applied Biosystems ...
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bioRxiv - Neuroscience 2020Quote: ... or WT N2aC plated in chamber slides using the Annexin V-FITC Early Apoptosis Detection Kit (Cell Signaling Technology, Danvers, MA). As a positive control for apoptosis and necrosis ...
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bioRxiv - Developmental Biology 2023Quote: ChIP-qPCR and ChIP-seq samples were prepared following the instructions of the SampleChIPR Enzymatic Chromatin IP Kit (Cell Signaling, #9005). Briefly ...
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bioRxiv - Neuroscience 2024Quote: ... Chromatin was sheared using a Covaris Sonicator for 20 minutes in sonication buffer (SimpleChIP Plus Sonication Kit, Cell Signaling Technology, #57976) to obtain fragments in the range of 200-500 bp ...
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bioRxiv - Cancer Biology 2022Quote: β-Galactosidase staining at pH 6.0 was performed with 5 × 105 cells using the Senescence β-Galactosidase Staining kit (Cell Signaling # C10841) according to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2023Quote: 25 mg cortical tissues were dissected from wild type mice (n = 3) and chromatin was prepared using the SimpleChIP plus Enzymatic Chromatin IP Kit (Cell Signaling). UBE3A-bound chromatin was immunoprecipitated using rabbit anti-UBE3A antibody (Bethyl Laboratories) ...
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bioRxiv - Microbiology 2023Quote: ... SARS-CoV-2 spike protein-specific IgG was measured using SARS-CoV2 spike protein serological IgG ELISA kit (Cell Signaling Technology) per manufacturer’s instructions.
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bioRxiv - Cancer Biology 2024Quote: ... Cell viability was measured after 24 hours of AraC treatment using the XTT Cell Viability Kit from Cell Signaling Technology (#9095) and a Tecan Infinite 200 Pro plate reader ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were stained for β-galactosidase (β-Gal) as a marker of senescence using a commercial kit (#9860S; Cell Signaling Technology) and imaged under an inverted brightfield microscope at 10X magnification ...
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bioRxiv - Immunology 2024Quote: ... The sections were incubated with anti-PLA2G12A antibody (clone #44) biotinylated with a Biotin-Labeling Kit (Dojindo) and rabbit anti-CD4 monoclonal antibody (D7D2Z; Cell Signaling) diluted at 1:1000 at room temperature for 1 h ...
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bioRxiv - Cancer Biology 2024Quote: Detection of SA-β-gal activity on adherent cells was done using the Senescence β-Galactosidase Staining Kit (#9860, Cell signaling), according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were fixed and stained for β-Galactosidase following the protocol of the Senescence β-Galactosidase Staining Kit (Cell Signaling Technologies). Following staining ...
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bioRxiv - Synthetic Biology 2024Quote: Senescence-associated β-galactosidase (SA-β-gal) staining was performed using the Senescence β-Galactosidase Staining Kit (Cell Signaling Technology, 9860). Briefly ...
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bioRxiv - Cancer Biology 2024Quote: Chromatin immunoprecipitation (ChIP) was performed using the SimpleChIP® Enzymatic Chromatin Magnetic Bead IP Kit per manufacturer’s protocol (Cell Signaling #9003). At least 4×106cells were plated in quadruplicate in 10 cm dishes and grown for ∼2 days until they began to form small spheres ...
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bioRxiv - Cell Biology 2020Quote: K-ε-GG remnant containing peptides were enriched using the PTMScan® Ubiquitin Remnant Motif (K-Є-GG) Kit (Cell Signaling Technology). Crosslinking of antibodies to beads and subsequent immunopurification was performed with slight modifications as previously described [62] ...
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bioRxiv - Neuroscience 2021Quote: Antibodies on these sections included Rat anti-CD3 epsilon (1:100, NBP1-26685, Novus Bio) and Rabbit anti-C-Kit (1:100, D13A2, Cell Signaling Tech).
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bioRxiv - Neuroscience 2020Quote: ... 5 days after irradiation the plates were assayed for senescence via the Senescence β-galactosidase Cell Staining Kit (Cell Signaling, Catalog # 9860) and the staining was performed as per manufacturer’s protocol.
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bioRxiv - Plant Biology 2020Quote: ... Immuno-precipitation was performed for two replicates for each genotype (about 1 g tissue/IP) by SimpleChIP Plus Kit (Cell Signaling Technology) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ChIP-seq experiments were performed with 108 HDFs per replicate using a SimpleChip Plus sonication chromatin IP kit (Cell Signaling Technology, 56383) according to the manufacturer directions ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti TRA-1-81 (1:200) and mouse anti-SSEA4 (1:200) (all from Cell Signaling Technology, StemLight Pluripotency Antibody Kit) to assess for pluripotency ...
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bioRxiv - Molecular Biology 2022Quote: Lysates from parental and resistant cells were used to screen activation of receptor tyrosine kinases using the PathScan® RTK Signaling Antibody Array Kit (7982, Cell Signaling) following the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2022Quote: ... Peptides were enriched for acetylation with anti-acetyl antibody conjugated to agarose beads (Acetyl-Lysine Motif Kit; Cell Signaling Technology, Danvers, MA). This process was performed according to the manufacturer protocol ...
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bioRxiv - Cancer Biology 2022Quote: Fragmental DNA for ChIP-seq was prepared using SimpleChIP®Plus Enzymatic Chromatin IP Kit (Magnetic Beads) (Cell Signaling Technologies, Cat#9005) per manufacturer’s instructions ...
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bioRxiv - Pathology 2021Quote: ... was used as secondary for 30 minutes and the signal was developed using the SignalStain DAB Substrate Kit (Cell Signaling, Danvers, MA).
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bioRxiv - Immunology 2020Quote: ... Cells were subsequently washed three times with ice-cold PBS and subcellular fractionation was carried out using a Cell Fractionation kit following manufacturer instructions (Cell Signaling, #9038).
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bioRxiv - Cell Biology 2021Quote: Acylated (either acetylated or malonylated) peptides were enriched by immunoaffinity purification using the PTMScan Acetyl-Lysine or PTMScan Malonyl-Lysine Kits (Cell Signaling Technology) according to the manufacturer’s protocol ...