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Citations for Cell Signaling Technology :
951 - 969 of 969 citations for Anion Exchange Membranes Thickness 10 13 µm since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... 10 mM sodium phosphate and 50 mM NaCl) and incubated with PTMScan pY antibody-conjugated beads (p-Tyr-1000, Cell Signaling Technology, USA) at a ratio of 4 µL slurry per mg protein at 4 °C for 2 h as previously described.43 Then ...
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bioRxiv - Cell Biology 2023Quote: ... 10% FBS) and incubated with the following primary antibodies in blocking buffer for 2 hours at RT: YAP (Cell Signaling Technology, #14074, 1:100), TAZ (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... Embryos were incubated for 1 hr in blocking buffer (PBS-Triton, FBS 10%) then overnight at 4°C with anti-Yap antibody (Cell Signaling, #4912, 1:200) in blocking buffer ...
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bioRxiv - Cell Biology 2024Quote: ... we incubated the cells with fluorescent secondary FAB fragments raised to the species corresponding to the primary antibody for 1 hr in PBS supplemented with 10% FBS (either Anti-rabbit IgG Fab2 Alexa Fluor ® 555 or Anti-mouse IgG Fab2 Alexa Flour ® 488, both Cell Signaling Technology, Catalogue # 647 4414 S and # 4408 S ...
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bioRxiv - Microbiology 2024Quote: ... The membrane was washed three times for 10 min each with 1x TBST and incubated with a horseradish peroxidase-conjugated secondary antibody (Cell Signaling Technology; Cat #7074S) for 1 h at room temperature ...
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bioRxiv - Immunology 2024Quote: ... removing PBS and conducting on-plate lysis by scraping the cells from each 10 cm plate into 600 μL of 1x lysis buffer (cat. #9803, Cell Signaling Technology, Danvers, MA) supplemented with 1 mM EDTA ...
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bioRxiv - Biochemistry 2024Quote: ... Anti- mouse, Cat. No. 7076; 1: 10 000 in 5% skim milk/TBS-T, Anti-rabbit, Cat. No. 7074S, Cell Signaling Technology, Massachusetts, US) and Pierce ECL Western Blotting Substrate (Cat ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were incubated with primary antibody in 10 % Donkey Serum in PBS overnight at 4 °C: Phospho-S6 Ribosomal Protein (Ser240/244) Rabbit mAb (Cell Signaling Technology, Cat# 5364; RRID:AB_10694233). After HSCs were washed three times with PBS + 0.1 % Tween-20 ...
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bioRxiv - Bioengineering 2021Quote: ... The membrane was washed three times with X1 TBST for 10 min each and incubated with HRP□conjugated rabbit or mouse secondary antibodies (#7074 and #7076, Cell Signaling Technology Inc., Danvers, MA) at 1:5000 dilution in X1 TBST with 5% nonfat dry milk for 1 h at RT ...
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bioRxiv - Biophysics 2020Quote: ... M-phase was discriminated with the phospho-Histone H3 (Ser-10) primary antibody (1:25 in PBS with 1% of NGS, Cell Signaling Technology, USA [RRID:AB 331748]) and the secondary anti-mouse IgG antibody (1:500 ...
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bioRxiv - Systems Biology 2022Quote: ... an Odyssey blocking buffer solution containing 10% OptiPrep™ and 1:2000 Alexa 488-conjugated anti-phospho-histone H3 (S10) antibody (pH3) (clone D2C8, Cell Signaling Technology, Danvers, MA) to label M-phase cells and 2 µg/ml Hoechst 33342 to stain all nuclei ...
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bioRxiv - Immunology 2024Quote: ... and kept at 4 °C for up to 10 days until chromatin fragmentation using SimpleChIP® Plus Sonication Chromatin IP Kit (Cell Signaling Technology, Danvers, MA). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting supernatants (10 μL) were analyzed by Western Blot with Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (Cell Signaling, 1679101S; dilution 1:1,000) primary antibodies ...
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bioRxiv - Cancer Biology 2024Quote: ... Blots were washed with TBST for 10 min three times before incubation with secondary antibodies [Anti-rabbit IgG-HRP (Cell Signaling Technology 7074S, 1:5000), Anti-mouse IgG- HRP (Cell Signaling Technology 7076S ...
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bioRxiv - Systems Biology 2023Quote: ... Membranes were then washed 3 x 10 minutes in TBS-T and incubated overnight in primary antibodies against phosphorylated Akt T308 (Cell Signaling Technologies #2965; diluted 1:1000), phosphorylated Akt S473 (Cell Signaling Technologies #9271 ...
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bioRxiv - Biochemistry 2021Quote: ... Membranes were blocked for 10 minutes in 10% BSA in TBS-T and then incubated overnight at 4°C with the indicated antibodies obtained from Cell Signaling Technology (phospho-PKC Substrate Motif (#6967), phospho-p42/44 MAPK (#4370) ...
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bioRxiv - Immunology 2020Quote: ... Fixed cells were labelled overnight with primary antibodies diluted in PBS 10% (w/v) BSA (for anti-RelA: rabbit monoclonal, Cell Signaling Technology #8242, 1:400; for phospho-p38: rabbit monoclonal Cell Signaling Technology #4511, 1:160000). The next day cells were washed 5x in PBS 10% (w/v) ...
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bioRxiv - Molecular Biology 2023Quote: ... and immunoprecipitation performed over night at 4 °C on a spinning wheel by adding 10 µl of the respective antibody (Anti H3K27Me3 #9733 Cell Signaling, Anti H3 #2650 Cell Signaling, Anti H3K56Ac #61061 Active Motif). Afterwards ...
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bioRxiv - Bioengineering 2023Quote: ... washed three times for 10 min in PBS and incubated for 1 h in blocking solution (5% normal goat serum, Cell Signaling Technology #5425, 0,3% Triton X-100 in PBS) at room temperature ...