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Citations for Cell Signaling Technology :
851 - 900 of 969 citations for Anion Exchange Membranes Thickness 10 13 µm since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Secondary antibodies used were: goat anti-rabbit IgG HRP (Cell Signaling Technology, 1:10 000, #7074), rabbit anti-goat IgG HRP (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: Cell lysates from 5-10 × 106 cells were generated using CHAPS Cell Extract Buffer (Cell Signaling Technology) supplemented with 1X Halt protease inhibitor cocktail (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... the inhibitor of MEK1/2/ERK1/2 kinases UO126 (used at 10 μM; 9903, Cell Signaling Technology), Smad3 inhibitor SIS3 (used at 6 μM ...
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bioRxiv - Microbiology 2021Quote: ... cells were incubated for 10 min on ice with Propidium Iodide Cell Staining Solution (Cell Signaling #4087) prior to flow cytometry.
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bioRxiv - Molecular Biology 2021Quote: ... 100 µl of magnetic beads and 10 µl of anti-p-RPS6 (Ser235/236) (Cell signaling #4857) were used per condition ...
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bioRxiv - Cell Biology 2020Quote: ... 10 uL of PTMScan® Ubiquitin Remnant Motif (K-ε-GG) Antibody Bead Conjugate purification (Cell Signaling) slurry was used per 1 mg peptide sample ...
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bioRxiv - Biochemistry 2022Quote: ... and blocked with IHC-blocking buffer (IHC-PBS-T with 10% normal goat serum (Cell Signaling Technology)) for 1 h at RT ...
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bioRxiv - Microbiology 2023Quote: ... 10 uL of PTMScan® Ubiquitin Remnant Motif (K-ε-GG) Antibody Bead Conjugate purification (Cell Signaling) slurry was used per 1 mg peptide sample ...
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bioRxiv - Cancer Biology 2023Quote: ... Supernatant was then mixed with 10 μg of α-TRF2 (NOVUS) or 10ug α-IgG (Cell Signaling) and 20 μL Protein G/agarose beads overnight at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... cells were lysed with the lysis buffer consisting of 10% cell lysis buffer (10x) (Cell Signaling #9803S), 1% protease inhibitor cocktail (100x ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 μg of chromatin DNA was incubated with rabbit anti-HIF1α (Cell Signaling Technology, cat no# 36169) or normal rabbit IgG (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2021Quote: ... and washed again three times for 10 min in TBST before exposure using ECL (SignalFire Plus, Cell signaling). The following antibodies and concentrations were used ...
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bioRxiv - Immunology 2023Quote: ... Sections were then incubated with rabbit anti-mouse Cleaved Caspase-3 (10) (Cell Signaling Technologies, catalog no. 9978S) at 1:100 dilution overnight at 4°C in 1% BSA ...
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bioRxiv - Molecular Biology 2023Quote: ... along with 10 μl of pre-washed Protein-A Agarose beads (Cat. No. 9863S, Cell Signaling, Danvers, MA). The tubes were left on a rotating disc at 40C overnight for pre-clearing the lysates ...
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bioRxiv - Bioengineering 2024Quote: ... which was thresholded using a positive and negative control for cell death (10 nM Staurosporine (Cell Signaling Technologies) or 1% FBS media) ...
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bioRxiv - Cancer Biology 2024Quote: ... Archived paraffin-embedded patient VS samples were stained with antibodies against phosphorylated TBK (1:10, Cell Signaling Technology). Four specimens of normal peripheral nerve were obtained postmortem and used as controls ...
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bioRxiv - Biochemistry 2024Quote: ... per sample were first conjugated with 4 μg (10 μL) mouse anti-myc-tag antibody (Cell Signaling, 9B11) for one hour at RT in a rotating wheel ...
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bioRxiv - Cancer Biology 2024Quote: ... Two milligrams of protein was immunoprecipitated at 4C overnight using either 10 ug of B1INT antibody (Cell Signaling), 20 uL of CCDC25 antibody/agarose conjugate or protein A/G PLUS agarose alone (Santa Cruz Biotechnology) ...
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bioRxiv - Cancer Biology 2021Quote: ... blocked with 10% goat serum diluted in PBS for 30 min and incubated with the CD44 antibody (Cell Signaling), cat#3570 ...
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bioRxiv - Genomics 2022Quote: ... H3K27me3 immunoprecipitation was performed on 4 million cells with 10 µL of H3K27me3 antibody (Cell Signaling, C36B11 Lot #19). H3K9me3 immunoprecipitation was performed on 3 million cells with 8 µL of H3K9me3 antibody (Cell Signaling ...
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bioRxiv - Immunology 2020Quote: ... 10 μM of p38 inhibitor (SB203580) or MEK1/2 inhibitor (U0126) was used (Cell Signaling Technology, Danvers, MA, USA). Cells were monitored by live cell imaging using the BD Pathway 855 Bioimaging System (BD Biosciences) ...
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bioRxiv - Immunology 2020Quote: ... that were pre-incubated with incubated with 10 ug of anti-STAT3 antibody (anti-Stat3, 12640S, Cell Signaling Technology) at 4°C overnight with gentle rotation ...
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bioRxiv - Developmental Biology 2020Quote: Cell lysates were prepared in 1x SDS Sample Buffer (62.5 mM Tris-HCl pH 6.8, 2%SDS, 42mM DTT, 10% glycerol and 0.01% bromophenol blue; Cell Signaling Technology), sonicated to shear DNA and reduce viscosity and then heat denatured ...
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bioRxiv - Developmental Biology 2022Quote: ... rat TS cells were exposed to a phosphatidylinositol 3-kinase (PI3K) inhibitor (LY294002, 10 μM, 9901, Cell Signaling Technology), chloroquine (50 μM for 24 h ...
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bioRxiv - Genetics 2024Quote: PANC1 and MDA-MB-231 cells were lysed for 10 min in 1X RIPA buffer (Cell Signaling Technology, #9806) supplemented with 1X Protease Inhibitor Cocktail (Roche ...
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bioRxiv - Developmental Biology 2022Quote: Whole testis tissue was homogenized in 1 ml per 100 mg tissue 1:10 RIPA buffer (Cell Signaling Technology Inc. ...
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bioRxiv - Neuroscience 2023Quote: ... 10% sucrose, 0.8 M NaCl, 1 mM EDTA, 0.1% sarkosyl and 1X protease/phosphatase inhibitor cocktail, #5872, Cell signaling) in a 15 ml glass homogenizer with 15 up and down strokes at 70% power by hand on ice ...
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bioRxiv - Neuroscience 2023Quote: ... blots were washed 3X 10 minutes with 1X TBST and probed with Horse Anti-Mouse IgG HRP (Cell Signaling) secondary antibody diluted in block for 1 hour at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... Lysates were incubated with 10 ug of anti-Nup98 antibody or an irrelevant isotype control (IgG DA1E, Cell signaling) overnight and subsequently incubated with protein G-beads for 2h ...
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bioRxiv - Immunology 2023Quote: ... IRF9 (6F1, hybridoma supernatants from Platanitis et al. 10, 1:20) and V5 (Cell signaling, Catalog # 13202, 1:2000), IRF1 (Cell signaling ...
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bioRxiv - Genetics 2023Quote: ... Red cells were lysed by resuspending the cells in 10 ml red cell lysis buffer (#46232, Cell Signaling Technology) for 10 minutes at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Protein lysates (1 mg) underwent overnight incubation with 10 µg of either PAK1 primary antibody (#2602, Cell Signaling Technology) or Rabbit IgG isotype control (#2729 ...
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Sir2 is required for the quiescence-specific condensed three-dimensional chromatin structure of rDNAbioRxiv - Molecular Biology 2024Quote: ... Pol II ChIPs were conducted with an antibody targeting the Rpb1 subunit (10 µL per reaction, Cell Signaling 2629S), similarly conjugated to 20 µL of protein G magnetic beads ...
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bioRxiv - Microbiology 2024Quote: ... 4°C for 10 mins and the obtained supernatant was incubated overnight with anti-V5 (#13202, Cell Signaling Technology) and Protein A-Sepharose beads at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... adipocytes were treated with assay medium +/-insulin for 10 minutes and harvested in cell lysis buffer (Cell Signaling Technology #9803) with phosphatase inhibitor (Thermo Fisher Scientific 78442) ...
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bioRxiv - Genomics 2021Quote: ... Rabbit anti-H3K27me3 (1:100 for bulk profiling or 1:10 for single-cell experimetns, Cell Signaling Technologies Cat# 9733S), Rabbit anti-H3K9me3 (1:100 ...
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bioRxiv - Cancer Biology 2022Quote: ... Two hundred microgram of chromatin was used for IP with 10 μl of STAT3 (1:50, Cat#12640, Cell Signaling) and 4 μg of IgG (1:250 ...
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Targeting adipocyte ESRRA promotes osteogenesis and vascular formation in adipocyte-rich bone marrowbioRxiv - Molecular Biology 2023Quote: ... These cells were then subjected to ChIP assays with primary antibody against ESRRA (10 μl/IP, Cell Signaling Technology #13826) or normal rabbit IgG ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed with PBS containing 1 mM EDTA and 1% FCS, resuspended in culture medium supplemented with Y27632 (10 μM, Gentaur) and DRAQ7 (1.5 μM, Cell Signaling), sorted based on Lgr5 and Sca-1 expression (and absence of DRAQ7 ...
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bioRxiv - Cell Biology 2023Quote: ... two 10-min washes on gyrotary shaker) then imaged by chemiluminescence with a HRP-conjugated secondary antibody (Cell Signaling 7074).
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bioRxiv - Microbiology 2020Quote: ... an amount of digested lysate equivalent to 10 mg of protein was subjected to ubiquitin remnant immunoprecipitation according to the manufacturer’s protocol (Cell Signaling Technologies). For phosphorylation analysis ...
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bioRxiv - Genetics 2021Quote: ... Blots were washed (3 × 10 mins) in TBST before and after incubating with a 1:10,000 anti-mouse secondary antibody (Cell Signaling, 7076S) overnight ...
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bioRxiv - Genetics 2021Quote: ... The blots were washed (3 × 10 mins) in TBST before and after incubating with a 1:10,000 anti-rabbit secondary antibody (Cell Signaling, 7074S). The blots were washed overnight before being incubated with a 1:5000 dilution of anti-HSP70-HRP (need catalog number ...
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bioRxiv - Genetics 2021Quote: ... Blots were washed (3 × 10 mins) in TBST before and after incubating with either a 1:10,000 anti-mouse secondary antibody (Cell Signaling, 7076S) or 1:10,000 anti-rabbit secondary antibody (Cell Signaling ...
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bioRxiv - Neuroscience 2020Quote: ... Chromatin solutions were incubated with 10 ug of anti-HIF1α (Cayman, Rabbit, #10006421) antibody or anti-rabbit IgG (Cell Signaling #2729) overnight at 4 °C ...
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bioRxiv - Cancer Biology 2020Quote: Human tissue samples and cell preparations were harvested in Complete RIPA Buffer - 1× radio immunoprecipitation buffer (10× RIPA; 9806, Cell Signaling) and one tablet each of PhoStop and Complete Mini (04906845001 and 04693124001 ...
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bioRxiv - Microbiology 2020Quote: ... an amount of digested lysate equivalent to 10 mg of protein was subjected to ubiquitin remnant immunoprecipitation according to the manufacturer’s protocol (Cell Signaling Technologies). For phosphorylation analysis ...
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bioRxiv - Neuroscience 2021Quote: ... Drosophila brains were fixed in 4% PFA for 10 min and prepared for staining according to the manufacturer’s protocol (Cell Signaling Technology). Brains were visualized by confocal microscopy (Zeiss LSM 780 NLO with Examiner ...
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bioRxiv - Pathology 2023Quote: ... fluorescence images were acquired on a Zeiss Axioscan Z7 at 20X magnification with a 10% tile overlap and analyzed using the Biology Image Analysis Software (BIAS, Cell Signaling). Keratinocytes were identified with a deep neural network on the basis of pan-cytokeratin ...
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bioRxiv - Cancer Biology 2023Quote: Cells were lysed after RA treatment for 48h with 1X lysis buffer (Cell Lysis Buffer 10 ×, Cell Signaling Technology, Danvers, USA), with protease and phosphatase inhibitors ...