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Citations for Cell Signaling Technology :
901 - 950 of 969 citations for Anion Exchange Membranes Thickness 10 13 µm since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... and immunoprecipitation performed over night at 4 °C on a spinning wheel by adding 10 µl of the respective antibody (Anti H3K27Me3 #9733 Cell Signaling, Anti H3 #2650 Cell Signaling ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were separated by 10-16% SDS-PAGE. Immunoblotting (Fig. S1b) was performed using rabbit monoclonal anti-GFP (D5.1) antibody (Cell Signaling Technology) at 1:1000 dilution.
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bioRxiv - Biochemistry 2024Quote: ... was first incubated in the chamber for 10 minutes followed by the addition of a biotinylated EphA2 or GFP antibody for 20 minutes (Cell Signaling and Rockland Immunochemical Inc. ...
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bioRxiv - Immunology 2024Quote: ... was added for 10 minutes to break the AMPK-Sestrin2 binding followed by incubation with the anti-Sestrin2 antibody (1:100, #8487, Cell Signaling) and secondary anti-rabbit IgG-HRP conjugated antibody (1:500) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... were stored as 10 mM stocks in DMSO. Antibodies for PARP (cat no. 9542) and GAPDH (cat no. 5174) were purchased from Cell Signaling. CCG-257081 enantiomers were prepared as described below ...
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bioRxiv - Developmental Biology 2024Quote: ... followed by blocking in PBST with 10% Horse Serum and incubation with rabbit anti-phospho-ser10-Histone3 (Cell Signaling Technology #3377S) at 1:500 ...
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bioRxiv - Cancer Biology 2024Quote: ... Sections were then blocked in 10% goat serum for 30 min at RT followed by incubation in primary antibody (Granzyme B [D2H2F], Cell Signaling) overnight at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... The cells were then washed with CyTOF permeabilization buffer and the barcoded samples were combined before being incubated with antibodies in a solution containing 10% NGS (Cell Signaling #5425). Cells were then washed with CyTOF staining buffer and fixed with 10% formalin (Merck #F5554 ...
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bioRxiv - Immunology 2021Quote: ... For phosphoflow experiments cells were immediately fixed by FoxP3/Transcription Factor Staining Buffer Set (eBioscience) for 10 minutes at 37°C after stimulation and labeled by pErk-AF488 (Cell Signaling, #13214) and pS6-APC (Cell Signaling ...
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bioRxiv - Cancer Biology 2020Quote: ... the slides were rinsed with PBS and then incubated with ready-to-use IHC detection reagent (Cell signaling, Danvers, MA; 10 µl) at room temperature for 1 h ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were washed for 10 minutes thrice with 1×TBST before incubation with horseradish peroxidase (HRP)-conjugated secondary antibodies (Goat Anti-Rabbit – Cell Signaling Technology, Danvers ...
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bioRxiv - Cancer Biology 2020Quote: ... and the whole fibrin gel channel was collected and immersed for 10 minutes on ice in 25 µL of cell lysis buffer (fresh stock of 10 mL prepared each time from 1X buffer (9803S, Cell Signaling Technologies) with the addition of 1 µL of Benzonase Nuclease (E8263 ...
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bioRxiv - Cancer Biology 2020Quote: ... Ten to 50 μg of proteins were separated by 10 % SDS-PAGE and visualized with primary Slug or E-cadherin antibody (C19G7, Cell Signaling Technology, #9585 ...
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bioRxiv - Neuroscience 2020Quote: ... Frozen brain lysates or hippocampi isolates were then homogenized with a T 10 basic ULTRA-TURRAX (IKa) in ice-cold buffer lysis (Cell Signaling 9803) and protease and phosphatase inhibitors (Roche) ...
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bioRxiv - Neuroscience 2020Quote: ... the slides were rinsed with PBS and then incubated with ready-to-use IHC detection reagent (Cell signaling, Danvers, MA; 10 μl) at room temperature for one hour ...
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bioRxiv - Genomics 2020Quote: ... The samples were blocked in 10% goat serum and incubated overnight at 4°C with the mouse Cas9 antibody (7A9-3A3 Cell Signaling Technologies) 1:800 dilution in 1% BSA (Supp ...
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bioRxiv - Microbiology 2020Quote: ... Lysates were then resolved on 10% sodium dodecyl sulfate polyacrylamide gels and blots were probed using the following antibodies: rabbit anti-FLAG (Cell Signaling Technology), rabbit anti-V5 (Invitrogen) ...
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bioRxiv - Neuroscience 2021Quote: ... The cells were permeabilized in 0.2% Triton X-100 for 10 min at room temperature following the incubation with anti-FLAG 1:1000 (Cell Signaling Technology, 14793s) and anti-Myc 1:1000 (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were permeabilized in 0.2% Triton X-100 for 10 mins at room temperature then incubated with anti-FLAG 1:1000 (Cell Signaling Technology, 14793s) and anti-Myc 1:1000 (Cell Signaling Technology ...
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bioRxiv - Immunology 2020Quote: ... Fixed cells were labelled overnight with primary antibodies diluted in PBS 10% (w/v) BSA (for anti-RelA: rabbit monoclonal, Cell Signaling Technology #8242 ...
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bioRxiv - Developmental Biology 2023Quote: ... in PBS and incubated overnight at 4 °C with primary antibody or isotype control antibody diluted in PBS containing 10% donkey serum (Jackson) or goat serum (Cell Signaling Technology). Secondary antibodies (1:1000 dilution ...
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bioRxiv - Physiology 2022Quote: ... The membranes were subsequently washed 4 times in TBS-T for 10 min before incubating in the appropriate secondary antibody anti-rabbit HRP (1:5000; 1677074P2, Cell Signaling Technology) or anti-mouse HRP (1:5000 ...
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bioRxiv - Genomics 2024Quote: ... Cell lysate was centrifuged at 21,000 x g for 10 minutes and supernatant was mixed with Blue Loading Buffer (Cell Signaling Technology #7722) per the manufacturer’s recommended ratio ...
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bioRxiv - Cancer Biology 2023Quote: ... and samples were prepared by boiling at 99 °C for 10 minutes with Blue Loading Buffer (7722S; Cell Signaling Technology, Danvers, MA). Then ...
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bioRxiv - Cell Biology 2022Quote: ... Fixed cells were blocked with 1% BSA plus 10% goat serum for 1 hr and stained with the anti-phospho-paxillin (Tyr118) antibody (Cell Signaling Technology) at 4°C overnight ...
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bioRxiv - Cell Biology 2023Quote: ... washed with TBST (3 ×10 min), incubated (1 hr, 20°C) in TBST containing HRP-conjugated secondary antibody (1:3000 anti-mouse, Cell Signaling Technology Cat# 7076S ...
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bioRxiv - Genomics 2024Quote: ... Nuclei resuspended in 1X PBS were then captured by adding 10 µl of preactivated Concanavalin A magnetic beads (Cell Signaling Technology # 93569) for 10 min with end-over-end mixing at RT ...
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bioRxiv - Plant Biology 2024Quote: ... Protein samples were separated using a 10% acrylamide SDS-PAGE gel and immunoblotted with anti-phospho-p44/42 MAPK (Erk1/2) (anti-pMAPK; Cell Signaling Technology).
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bioRxiv - Bioengineering 2024Quote: ... 45 µL of cells suspension were incubated for 90 min at RT in a total volume of 55 µL with 10 nM BioGCSFR and 1:200 Myc-Tag (9B11) Mouse mAb (2276, Cell Signaling Technology). After one wash with 500 µL PBS ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were rinsed three times for 10 minutes and then incubated with horseradish peroxidase (HRP)-conjugated anti-rabbit or anti-mouse antibodies (1:2,500, Cell Signaling, #7074, #7076) at room temperature for 1 hour ...
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bioRxiv - Plant Biology 2024Quote: ... Twenty µl was saved as input and the remaining supernatant was then incubated at 4°C overnight with specific antibodies (5 µg of H3K27me3 Ab, Diagenode C15410069 or Millipore 07-449; 10 µl H2AUb Ab, Cell Signaling Technology ref.8240 ...
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bioRxiv - Cell Biology 2024Quote: ... The membrane was washed three times with TBS-T for 10 min each at room temperature and then incubated with HRP-conjugated secondary antibodies (Cell Signaling Technologies) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... The precleared lysate was reserved and incubated with 10 µL of a 1:50 dilution of rabbit anti-HA antibody (C29F4, Cell Signaling Technology) for 2-hr at RT ...
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bioRxiv - Developmental Biology 2024Quote: ... cells were fixed in 4% PFA for 10 minutes and processed for immunostaining by standard methods using combinations of rabbit (Cell Signaling, 71D10) or mouse anti-Myc (Cell Signaling ...
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bioRxiv - Molecular Biology 2020Quote: ... Final cell pellets were lysed on ice for 10 min in 1X RIPA buffer (cat. no. 9806S, Cell Signaling Technology, Danvers, MA, USA) with added protease inhibitors (cat ...
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bioRxiv - Bioengineering 2021Quote: ... washed three times for 10 min in PBS and incubated for 1 hour in blocking solution (5% normal goat serum (Cell Signaling Technology #5425), 0,3% Triton X-100 in PBS ...
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bioRxiv - Biochemistry 2020Quote: ... The membrane was then washed with 1× TBST (3 × 10 min) and incubated with anti-mouse IgG horseradish-peroxidase secondary antibody conjugate (1:10000 dilution; Cell Signaling Technology, 7076S) in 1× TBST for 1 h at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... sections were washed with Bond Wash solution 2x 10s and blocked with a phosphate-buffered saline (PBS) solution containing 5% normal goat serum (5425S, Cell Signaling, Danvers, MA) and 0.5% TritonX-100 (T8787 ...
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bioRxiv - Biochemistry 2020Quote: ... The membrane was washed 1× TBST three times for 10 min each followed by incubation with anti-rabbit IgG horseradish-peroxidase secondary antibody conjugate (1:3000 dilution; Cell Signaling Technology, 7074S) in 1× TBS for 2 h ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein bound beads were boiled with 1X SDS loading buffer and resolved in 10% SDS-PAGE and analyzed by with 1:1000 dilution of rabbit anti-PAX9 polyclonal antibody (Cell Signaling Technology, USA) or 1:1000 dilution of mouse anti-FLAGM2 monoclonal antibody (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: Co-immunoprecipitation of endogenous PARP1 and SET8 were performed with 200 µg of total extract from crosslinked (1% formaldehyde for 10 min) HCT116 or HeLa cells using anti-PARP1 antibody (Cell Signaling Technology # 9532), anti-SET8 antibody (Santa Cruz Biotechnology # sc-515433 ...
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bioRxiv - Neuroscience 2023Quote: ... with 10% horse serum, 1% penicillin-streptomycin (gibco, Cat# 15140-122) and 0.1 ug/mL nerve growth factor (NGF, Cell signaling Technologies, Cat# 84087S). An additional 2-3 mL of culture media was added ...
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bioRxiv - Molecular Biology 2023Quote: ... Beads were then suspended in 50 µl Na-Phosphate buffer and incubated at room temperature for 10 minutes with desired antibody [20 µl of CTCF antibody (Cell Signaling Technology #2899) or 11 µg of anti-rabbit IgG (Thermo)] ...
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bioRxiv - Molecular Biology 2023Quote: Cells were washed with D-PBS (-) (Wako, 045-29795) and lysed using 140 µL of 10× Cell Lysis buffer (Cell Signaling Technology, 9803S) with PMSF (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2023Quote: ... The membrane was then washed in TBS-Tween three times for 10 min at RT and incubated with the secondary antibody anti-mouse-HRP (1:5000) (Cell Signaling, Danvers, MA) for one hour at RT ...
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bioRxiv - Microbiology 2024Quote: ... buffer (1% FBS in 1x PBS) on ice for 10 minutes and then stained for viability (Ghost DyeTM Red 780; Cell Signaling, Cat# 18452S) on ice for 20 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... Histone modification ChIPs were performed by using 10 µg of chromatin each incubated with the recommended amount of H3K27Ac (Cell Signaling #8173, 5ul), H3K4me1 (Cell Signaling #5326 ...
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bioRxiv - Genomics 2023Quote: ... Cells were proceeded for IF as described above using anti-CD56 mouse antibodies (1:10, Coulter #PN4235479-G) and anti-TCF7L2 rabbit antibodies (1:100, Cell Signaling Technology #C48H11), revealed by Cy3-coupled anti-mouse IgGs and Cy5-coupled anti-rabbit IgGs.
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bioRxiv - Neuroscience 2024Quote: ... Fixed cultures were permeabilized with 0.1% Triton-x in PBS 10 minutes and blocked in blocking buffer (5% Normal Goat Serum (Cell Signaling Tech. cat#: 5425S) in 1x PBS ...
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bioRxiv - Neuroscience 2024Quote: ... For serial staining of sections (dual IHC) a stripping step was performed by boiling slides for 10 min in a 10mM sodium citrate buffer (Cell Signaling cat# 14746). Following stripping ...