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• Liraglutide protects against diastolic dysfunction and improves ventricular protein translation

  Cody Rutledge, et al., bioRxiv - Physiology 2023

  Quote: ... for 1 hour and then incubated overnight at 4°C with primary antibodies p-S6 (Ser240/244, 1:1000, Cell Signaling, #2215), S6 (1:1000 ...
• Liraglutide protects against diastolic dysfunction and improves ventricular protein translation

  Cody Rutledge, et al., bioRxiv - Physiology 2023

  Quote: ... S6 (1:1000, Cell Signaling, #2217), GAPDH (1:5000 ...
• Liraglutide protects against diastolic dysfunction and improves ventricular protein translation

  Cody Rutledge, et al., bioRxiv - Physiology 2023

  Quote: ... phospho-BCKDH (Cell Signaling #40368, 1:1000) and BCKDH (Cell Signaling #90198 ...
• Liraglutide protects against diastolic dysfunction and improves ventricular protein translation

  Cody Rutledge, et al., bioRxiv - Physiology 2023

  Quote: ... and BCKDH (Cell Signaling #90198, 1:1000). Following incubation ...
• Anticancer efficacy of KRASG12C inhibitors is potentiated by PAK4 inhibitor KPT9274 in preclinical models of KRASG12C mutant pancreatic and lung cancers

  Husain Yar Khan, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Incubation with 1:2000 diluted HRP-linked secondary antibodies (# 7074/7076; Cell Signaling, Danvers, MA, USA) in 3% non-fat dry milk was subsequently performed at room temperature for 1 hour ...
• Acetyl transferase EP300 deficiency leads to chronic replication stress mediated by defective fork protection at stalled replication forks

  Angelica Barreto-Galvez, et al., bioRxiv - Cancer Biology 2023

  Quote: ... anti-H3 (Cell Signaling – 12648T), anti-H3K27ac (Cell Signaling 8173T) ...
• Acetyl transferase EP300 deficiency leads to chronic replication stress mediated by defective fork protection at stalled replication forks

  Angelica Barreto-Galvez, et al., bioRxiv - Cancer Biology 2023

  Quote: ... anti-H3K27ac (Cell Signaling 8173T), anti-EP300 (ABCAM-ab10485) ...
• Acetyl transferase EP300 deficiency leads to chronic replication stress mediated by defective fork protection at stalled replication forks

  Angelica Barreto-Galvez, et al., bioRxiv - Cancer Biology 2023

  Quote: ... anti-yH2AX (Cell Signaling – 9718T), anti-pCHK1 (s317 ...
• Acetyl transferase EP300 deficiency leads to chronic replication stress mediated by defective fork protection at stalled replication forks

  Angelica Barreto-Galvez, et al., bioRxiv - Cancer Biology 2023

  Quote: ... and then with an Alexa Fluor 488 secondary antibody (Cell Signaling Technology) for 60 min at RT ...
• Acetyl transferase EP300 deficiency leads to chronic replication stress mediated by defective fork protection at stalled replication forks

  Angelica Barreto-Galvez, et al., bioRxiv - Cancer Biology 2023

  Quote: ... anti-pCHK1 (s317) (Cell signaling –123402), anti-pRPA (ser4 ...
• Acetyl transferase EP300 deficiency leads to chronic replication stress mediated by defective fork protection at stalled replication forks

  Angelica Barreto-Galvez, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Slides were then incubated with primary antibody for Phospho-Histone H2A.X (Ser139) (Cell Signaling Technology) overnight at 4°C ...
• Regenerative growth is constrained by brain tumor to ensure proper patterning in Drosophila

  Syeda Nayab Fatima Abidi, et al., bioRxiv - Developmental Biology 2023

  Quote: ... and anti-cleaved Dcp-1 (1:250)(Cell Signaling #9578). The Developmental Studies Hybridoma Bank (DSHB ...
• Genetically diverse mouse models of SARS-CoV-2 infection reproduce clinical variation in type I interferon and cytokine responses in COVID-19

  Shelly J. Robertson, et al., bioRxiv - Microbiology 2023

  Quote: ... SARS-CoV-2 spike protein-specific IgG was measured using SARS-CoV2 spike protein serological IgG ELISA kit (Cell Signaling Technology) per manufacturer’s instructions.
• Enhanced cell viscosity: a new phenotype associated with lamin A/C alterations

  Cécile Jebane, et al., bioRxiv - Biophysics 2023

  Quote: ... and β-galactosidase activity was measured following the manufacturer’s instructions (Senescence β-Galactosidase Staining Kit, Cell Signaling Technology).
• Biofunctional matrix models reveal mineral-dependent mechanoregulation of bone metastatic breast cancer

  Siyoung Choi, et al., bioRxiv - Bioengineering 2023

  Quote: ... stained using rabbit anti-human Ki67 monoclonal antibody (Cell Signaling Technologies, US), detected with goat anti-rabbit Alexa Fluor 568 secondary antibody (Thermo Fischber Scientific ...
• Targeting hypoxia-induced circSTT3A decreases breast cancer stem cell formation via degradation of PGK1 protein and serine synthesis

  Ming Xu, et al., bioRxiv - Cancer Biology 2023

  Quote: ... membranes were incubated with primary antibodies described as follow: HSP70 (1:1000, Cell Signaling Technology), PGK1 (1:1000 ...
• Colorectal cancer detection and treatment with engineered probiotics

  Candice R. Gurbatri, et al., bioRxiv - Bioengineering 2023

  Quote: ... and stained with either H&E or specific immunohistochemistry markers (HA-Tag C29F4 #3724 from Cell Signaling Technology; GranzymeB Leica Biosystems PA0291 ...
• Dose-response relationship for the resistance of human insulin to degradation by insulin-degrading enzyme

  Masaki Okumura, et al., bioRxiv - Biochemistry 2023

  Quote: ... anti-AKT (#9272, Cell Signaling Technology), or anti-GAPDH (#2118 ...
• STING activation of IRF3 is tuned by PELI2 to suppress basal activation and reinforce the anti-viral response

  Christopher Ritchie, Lingyin Li, bioRxiv - Biochemistry 2023

  Quote: ... Cells were then pelleted and lysed in 400 μL lysis buffer (Cell Signaling Technology) supplemented with protease and phosphatase inhibitors (Roche) ...
• Unraveling the Potential Role of Tecomella undulata in Experimental NASH

  Akshatha N. Srinivas, et al., bioRxiv - Physiology 2023

  Quote: ... and β-actin (Cell Signaling). Upon overnight incubation at 40C ...
• Nanoscale engagement and clusterization of Programmed death ligand 1 (PD-L1) in the membrane lipid rafts of Non-Small Cell Lung Cancer cells

  Martina Ruglioni, et al., bioRxiv - Biophysics 2023

  Quote: αPD-L1: Rabbit anti-PD-L1 monoclonal IgG (mAb D8T4X, #86744 Cell Signaling, Euroclone, Milan, Italy). Immunolabeling dilution ...
• Nanoscale engagement and clusterization of Programmed death ligand 1 (PD-L1) in the membrane lipid rafts of Non-Small Cell Lung Cancer cells

  Martina Ruglioni, et al., bioRxiv - Biophysics 2023

  Quote: i647: rabbit IgG Isotype Control AlexaFluor647-conjugated (DA1E, #2985, Cell Signaling Technologies). Immunolabeling dilution ...
• Nanoscale engagement and clusterization of Programmed death ligand 1 (PD-L1) in the membrane lipid rafts of Non-Small Cell Lung Cancer cells

  Martina Ruglioni, et al., bioRxiv - Biophysics 2023

  Quote: αPD-L1-647: Rabbit anti-PD-L1 monoclonal IgG conjugated to AlexaFluor647 ( D8T4X, #41726, Cell Signaling, Euroclone, Milan, Italy). Immunolabeling dilution ...
• Endosome dysfunction leads to gain-of-function TLR7 and human lupus

  Harshita Mishra, et al., bioRxiv - Immunology 2023

  Quote: ... anti-LC3b (1:1000,clone D11,3868,Cell Signaling), anti-Rab7 (1:1000 ...
• Endosome dysfunction leads to gain-of-function TLR7 and human lupus

  Harshita Mishra, et al., bioRxiv - Immunology 2023

  Quote: ... anti-Phospho-p38 (1:1000, D3F9, 4511T, Cell Signaling), anti-p38 (1:1000 ...
• Endosome dysfunction leads to gain-of-function TLR7 and human lupus

  Harshita Mishra, et al., bioRxiv - Immunology 2023

  Quote: ... anti-Rab7 (1:1000, D95F2, 9367, Cell Signaling), anti-Rab5 (1:1000 ...
• Endosome dysfunction leads to gain-of-function TLR7 and human lupus

  Harshita Mishra, et al., bioRxiv - Immunology 2023

  Quote: ... anti-Phospho-p44/42 (ERK1/2) (1:1000, 197G2, 4377T, Cell Signaling), anti-p44/42 (ERK1/2 ...
• Endosome dysfunction leads to gain-of-function TLR7 and human lupus

  Harshita Mishra, et al., bioRxiv - Immunology 2023

  Quote: ... anti-EEA1 (1:1000, clone C45B10, 3288S, Cell Signaling), anti-LC3b (1:1000,clone D11,3868,Cell Signaling) ...
• Endosome dysfunction leads to gain-of-function TLR7 and human lupus

  Harshita Mishra, et al., bioRxiv - Immunology 2023

  Quote: ... anti-Rab5 (1:1000, C8B1, 3547, Cell Signaling). Secondary antibodies ...
• Endosome dysfunction leads to gain-of-function TLR7 and human lupus

  Harshita Mishra, et al., bioRxiv - Immunology 2023

  Quote: ... anti-p38 (1:1000, D13E1, 8690T, Cell Signaling), anti-Phospho-p44/42 (ERK1/2 ...
• Endosome dysfunction leads to gain-of-function TLR7 and human lupus

  Harshita Mishra, et al., bioRxiv - Immunology 2023

  Quote: ... anti-IκBα (1:1000, L35A5, 4814, Cell Signaling), anti-Borcs5 (1:500 ...
• Endosome dysfunction leads to gain-of-function TLR7 and human lupus

  Harshita Mishra, et al., bioRxiv - Immunology 2023

  Quote: ... anti-Rab7 (1:100, D95F2, 9367, Cell Signaling), anti-Rab5(1:200 ...
• Mapping of the podocin proximity-dependent proteome reveals novel components of the kidney podocyte foot process

  Gary F. Gerlach, et al., bioRxiv - Developmental Biology 2023

  Quote: ... Primary antibodies (Rabbit anti-HA tag (Cell Signaling, 3724S [1:500] ...
• Induction of PARP7 Creates a Vulnerability for Growth Inhibition by RBN2397 in Prostate Cancer Cells

  Chunsong Yang, et al., bioRxiv - Cancer Biology 2023

  Quote: ... p-STAT1(Tyr701)(1:1,000, rabbit, Cell Signaling 9171), LAMIN A/C (1:40 ...
• FH variant pathogenicity promotes purine salvage pathway dependence in kidney cancer

  Blake R. Wilde, et al., bioRxiv - Cancer Biology 2023

  Quote: ... The following commercially available antibodies were used: GFP (Cell Signaling Technology Cat# 2956 ...
• Distinct Nrf2 Signaling Thresholds Mediate Lung Tumor Initiation and Progression

  Janine M. DeBlasi, et al., bioRxiv - Cancer Biology 2023

  Quote: ... NRF2 (Cell Signaling Technologies, D1Z9C, RRID:AB_2715528, 1:1000), NQO1 (Sigma Aldrich ...
• FH variant pathogenicity promotes purine salvage pathway dependence in kidney cancer

  Blake R. Wilde, et al., bioRxiv - Cancer Biology 2023

  Quote: ... FH (Cell Signaling Technology Cat# 4567 ...
• Distinct Nrf2 Signaling Thresholds Mediate Lung Tumor Initiation and Progression

  Janine M. DeBlasi, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Ki-67 (Cell Signaling Technology, RRID:AB_2620142, 1:200), and Cleaved Caspase-3 (Cell Signaling Technology ...
• Biofunctional matrix models reveal mineral-dependent mechanoregulation of bone metastatic breast cancer

  Siyoung Choi, et al., bioRxiv - Bioengineering 2023

  Quote: ... Paraffin sections were used for H&E staining as well as sequential double-immuno-staining with rabbit anti-human Ki67 (Cell Signaling Technologies, US) and rabbit anti-human vimentin (Thermo Fisher Scientific ...
• The Sequence Basis for Selectivity of Ephrin-B2 Ligand for Eph Receptors and Pathogenic Henipavirus G Glycoproteins

  Krishna K. Narayanan, et al., bioRxiv - Microbiology 2023

  Quote: ... the cells were incubated with anti-myc Alexa 647 (clone 9B11, 1/250 dilution, Cell Signaling Technology) in PBS-BSA for 20 minutes at 4°C ...
• STING activation of IRF3 is tuned by PELI2 to suppress basal activation and reinforce the anti-viral response

  Christopher Ritchie, Lingyin Li, bioRxiv - Biochemistry 2023

  Quote: ... then lysed in 400 μL lysis buffer (Cell Signaling Technology) supplemented with protease and phosphatase inhibitors (Roche) ...
• STING activation of IRF3 is tuned by PELI2 to suppress basal activation and reinforce the anti-viral response

  Christopher Ritchie, Lingyin Li, bioRxiv - Biochemistry 2023

  Quote: ... 30 μL of protein A magnetic agarose (Cell Signaling Technology) was added incubated for an additional 4 h rotating at 4 °C ...
• Acetyl transferase EP300 deficiency leads to chronic replication stress mediated by defective fork protection at stalled replication forks

  Angelica Barreto-Galvez, et al., bioRxiv - Cancer Biology 2023

  Quote: ... anti-CHK1 (Cell Signaling - 2360), anti-RPA (Bethyl – A300-244A) ...
• Gut barrier defects, increased intestinal innate immune response, and enhanced lipid catabolism drive lethality in N-glycanase 1 deficient Drosophila

  Ashutosh Pandey, et al., bioRxiv - Developmental Biology 2023

  Quote: ... The following antibodies were used: rabbit anti-pFoxo1 1:1000 (Cat No. 9461, Cell Signaling Technology), rabbit anti-Foxo1 1:1000 (Cat No ...
• Gut barrier defects, increased intestinal innate immune response, and enhanced lipid catabolism drive lethality in N-glycanase 1 deficient Drosophila

  Ashutosh Pandey, et al., bioRxiv - Developmental Biology 2023

  Quote: ... rabbit anti-Foxo1 1:1000 (Cat No. 2880, Cell Signaling Technology), rabbit anti-Akt 1:1000 (Cat No ...
• Gut barrier defects, increased intestinal innate immune response, and enhanced lipid catabolism drive lethality in N-glycanase 1 deficient Drosophila

  Ashutosh Pandey, et al., bioRxiv - Developmental Biology 2023

  Quote: ... rabbit anti-Akt 1:1000 (Cat No. 4691, Cell Signaling Technology), rabbit anti-pAkt 1:1000 (cat No ...
• A cross-species proteomic map reveals neoteny of human synapse development

  Li Wang, et al., bioRxiv - Neuroscience 2023

  Quote: ... SYN1 (Cell Signaling Technology, 5297S, 1:250), DLG4 (Synaptic Systems ...
• Schizophrenia-associated NRXN1 deletions induce developmental-timing- and cell-type-specific vulnerabilities in human brain organoids

  Rebecca Sebastian, et al., bioRxiv - Neuroscience 2023

  Quote: ... rabbit anti-SOX2 (1:500 Cell Signaling 3697S), rabbit anti-HOPX (1:500 ...
• Dissecting endocytic mechanisms reveals new molecular targets to enhance sodium iodide symporter activity with clinical relevance to radioiodide therapy

  Martin L. Read, et al., bioRxiv - Cancer Biology 2023

  Quote: ... PICALM (1:1000; Cell Signaling Technology) and β-actin (1:2000 ...
• Dissecting endocytic mechanisms reveals new molecular targets to enhance sodium iodide symporter activity with clinical relevance to radioiodide therapy

  Martin L. Read, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Na,K-ATPase (1:500; Cell Signaling Technology); PICALM (1:1000 ...