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• Modelling drug responses and evolutionary dynamics using triple negative breast cancer patient-derived xenografts

  Abigail Shea, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Vimentin (Cell Signaling Technology, 5741S, 1:1000) and β-actin (Sigma-Aldrich ...
• A novel role of USP7 in imparting partial EMT state in colorectal cancer through the DDX3X-β-catenin axis

  Bhaskar Basu, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Vimentin (Cell Signaling Technology), USP7 ...
• Modelling drug responses and evolutionary dynamics using triple negative breast cancer patient-derived xenografts

  Abigail Shea, et al., bioRxiv - Cancer Biology 2023

  Quote: ... and β-tubulin (Cell Signaling Technology, 2146S, 1:1000) primary antibodies ...
• Modelling drug responses and evolutionary dynamics using triple negative breast cancer patient-derived xenografts

  Abigail Shea, et al., bioRxiv - Cancer Biology 2023

  Quote: ... blocked in Tris-buffered saline/0.1% Tween (TBST)/5% milk and probed with antibodies against E-Cadherin (Cell Signaling Technology, 3195S, 1:1000 dilution), Vimentin (Cell Signaling Technology ...
• The cancer-specific lncRNA LISR customizes ribosomes to suppress anti-tumour immunity

  Sonia Cinque, et al., bioRxiv - Cancer Biology 2023

  Quote: ... ATF4 (#11815, clone D4B8; Cell Signaling Technology, 1:500), eIF2α-Tot (#5324 ...
• Oncogene-like addiction to aneuploidy in human cancers

  Vishruth Girish, et al., bioRxiv - Cancer Biology 2023

  Quote: Senescence-associated β-galactosidase staining was performed using a senescence β-galactosidase staining kit (Cell Signaling Technology, cat. no. 9860) according to the manufacturer’s instructions ...
• Oncogene-like addiction to aneuploidy in human cancers

  Vishruth Girish, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Cleaved-PARP (Asp214) (Cell Signaling; cat. no. 5625S) at a dilution of 1:1000 in 5% milk ...
• Preneoplastic cells switch to Warburg metabolism from their inception exposing multiple vulnerabilities for targeted elimination

  Henna Myllymäki, et al., bioRxiv - Cancer Biology 2023

  Quote: ... eGFP immunostaining was performed with rabbit monoclonal anti-GFP antibody (1:200; cat. 2956, Cell Signaling Technology, London, UK) and Alexa Fluor 488 Goat anti-Rabbit secondary antibody (1:250 ...
• ERBB4-mediated signaling is a mediator of resistance to BTK and PI3K inhibitors in B cell lymphoid neoplasms

  Alberto J. Arribas, et al., bioRxiv - Cancer Biology 2023

  Quote: ... The primary antibodies used included rabbit monoclonal anti human p-AKT Ser473 (1:1000, Cell signaling), NF-κB/p65 (E379 ...
• USP8 inhibition promotes Parkin-independent mitophagy in the Drosophila brain and in human neurons

  Sofia Mauri, et al., bioRxiv - Cell Biology 2023

  Quote: ... Precoupled anti-KGG beads (Cell Signaling Technology) were washed in 1X IAP buffer before contacting the digested peptides ...
• Tipifarnib potentiates the antitumor effects of PI3Kα inhibition in PIK3CA- and HRAS-dysregulated HNSCC via convergent inhibition of mTOR activity

  Alison E. Smith, et al., bioRxiv - Cancer Biology 2023

  Quote: ... resuspending in 1X cell lysis buffer (Cell Signaling Technology #9803) or RIPA buffer supplemented with Halt protease inhibitor cocktail (Thermo Scientific #78430 ...
• RNA sequencing identifies lung cancer lineage and facilitates drug repositioning

  Longjin Zeng, et al., bioRxiv - Cancer Biology 2023

  Quote: ... The antibodies used include rabbit anti-KLF5 polyclonal antibody (Cell Signaling Technology), and rabbit anti-β-actin polyclonal antibody (BL005B ...
• Recurrent phases of strict protein limitation inhibit tumor growth and restore lifespan in a Drosophila intestinal cancer model

  Roxana M. Pfefferkorn, et al., bioRxiv - Cancer Biology 2023

  Quote: ... The intestines were dissected and fixed in 4% paraformaldehyde for 1 h before blocking with 5% NGS and staining with rabbit-PH3 primary antibody (Cell Signaling Technology, Leiden, Netherlands, #3377) overnight ...
• RBM43 links adipose inflammation and energy expenditure through translational regulation of PGC1α

  Phillip A. Dumesic, et al., bioRxiv - Cell Biology 2023

  Quote: ... TBP (44059S, Cell Signaling Technology); RPS3 (PA517214 ...
• UCHL1-dependent control of Hypoxia-Inducible Factor Transcriptional Activity in Liver Disease

  Amy Collins, et al., bioRxiv - Cell Biology 2023

  Quote: ... rodent HIF1a (#14179, Cell Signaling Technologies), Hydroxy-HIF-1α (Pro564 ...
• UCHL1-dependent control of Hypoxia-Inducible Factor Transcriptional Activity in Liver Disease

  Amy Collins, et al., bioRxiv - Cell Biology 2023

  Quote: ... VHL (#68547, Cell Signaling Technologies), ubiquitin (sc-8017 ...
• UCHL1-dependent control of Hypoxia-Inducible Factor Transcriptional Activity in Liver Disease

  Amy Collins, et al., bioRxiv - Cell Biology 2023

  Quote: ... Hydroxy-HIF-1α (Pro564) (#3434, Cell Signaling Technologies), VHL (#68547 ...
• YAP-dependent autophagy is controlled by AMPK, SIRT1 and flow intensity in kidney epithelial cells

  Aurore Claude-Taupin, et al., bioRxiv - Cell Biology 2023

  Quote: ... Phospho-YAP_S61 (Cell Signaling Technology, #75784, 1:1000), TAZ (Sigma ...
• YAP-dependent autophagy is controlled by AMPK, SIRT1 and flow intensity in kidney epithelial cells

  Aurore Claude-Taupin, et al., bioRxiv - Cell Biology 2023

  Quote: ... Phospho-YAP_S127 (Cell Signaling Technology, #13008, 1:1000), Phospho-YAP_S61 (Cell Signaling Technology ...
• YAP-dependent autophagy is controlled by AMPK, SIRT1 and flow intensity in kidney epithelial cells

  Aurore Claude-Taupin, et al., bioRxiv - Cell Biology 2023

  Quote: ... LATS1 (Cell Signaling Technology, #9153, 1:1000), Phospho-LATS1_T1079 (Cell Signaling Technology ...
• YAP-dependent autophagy is controlled by AMPK, SIRT1 and flow intensity in kidney epithelial cells

  Aurore Claude-Taupin, et al., bioRxiv - Cell Biology 2023

  Quote: ... sections were incubated with YAP (Cell Signaling Technology, #14074, 1:200) antibody overnight at 4°C ...
• YAP-dependent autophagy is controlled by AMPK, SIRT1 and flow intensity in kidney epithelial cells

  Aurore Claude-Taupin, et al., bioRxiv - Cell Biology 2023

  Quote: ... Phospho-LATS1_T1079 (Cell Signaling Technology, #8654, 1:1000), H3K9ac (Sigma ...
• YAP-dependent autophagy is controlled by AMPK, SIRT1 and flow intensity in kidney epithelial cells

  Aurore Claude-Taupin, et al., bioRxiv - Cell Biology 2023

  Quote: ... Phospho-TAZ_S89 (Cell Signaling Technology, #59971, 1:1000), LATS1 (Cell Signaling Technology ...
• Apo- and holo- transferrin differentially interact with ferroportin and hephaestin to regulate iron release at the blood-brain barrier

  Stephanie L. Baringer, et al., bioRxiv - Cell Biology 2023

  Quote: ... TfR (Cell Signaling, 13208S, 1:500), Tf (Abcam ...
• YAP-dependent autophagy is controlled by AMPK, SIRT1 and flow intensity in kidney epithelial cells

  Aurore Claude-Taupin, et al., bioRxiv - Cell Biology 2023

  Quote: ... YAP (Cell Signaling Technology, #4912, 1:1000), Phospho-YAP_S127 (Cell Signaling Technology ...
• YAP-dependent autophagy is controlled by AMPK, SIRT1 and flow intensity in kidney epithelial cells

  Aurore Claude-Taupin, et al., bioRxiv - Cell Biology 2023

  Quote: ... 10% FBS) and incubated with the following primary antibodies in blocking buffer for 2 hours at RT: YAP (Cell Signaling Technology, #14074, 1:100), TAZ (Sigma ...
• Targeting the deNEDDylating enzyme NEDP1 to ameliorate ALS phenotypes through Stress Granules dissolution

  Toufic Kassouf, et al., bioRxiv - Cell Biology 2023

  Quote: ... (#3925, Cell Signaling), secondary antibodies (anti-mouse #4416 ...
• Targeting the deNEDDylating enzyme NEDP1 to ameliorate ALS phenotypes through Stress Granules dissolution

  Toufic Kassouf, et al., bioRxiv - Cell Biology 2023

  Quote: Di-Glycine motif peptide identification was performed by Cell Signaling Technology, following UbiScan protocols and instructions (Cell Signaling Technology ...
• MANF stimulates autophagy and restores mitochondrial homeostasis to treat toxic proteinopathy

  Yeawon Kim, et al., bioRxiv - Cell Biology 2023

  Quote: ... or anti-mouse GAPDH antibody (Cell Signaling, Danvers, MA). Relative intensities of protein bands were quantified using Image J (NIH ...
• MANF stimulates autophagy and restores mitochondrial homeostasis to treat toxic proteinopathy

  Yeawon Kim, et al., bioRxiv - Cell Biology 2023

  Quote: ... or a rabbit anti-human P62 antibody (Cell Signaling) in combination with a mouse IgG2b antibody against human uromodulin (RayBiotech) ...
• Targeting the deNEDDylating enzyme NEDP1 to ameliorate ALS phenotypes through Stress Granules dissolution

  Toufic Kassouf, et al., bioRxiv - Cell Biology 2023

  Quote: ... mouse anti-tubulin (#3873, Cell Signaling), sheep anti-NEDP1 (in house)(38) ...
• Targeting the deNEDDylating enzyme NEDP1 to ameliorate ALS phenotypes through Stress Granules dissolution

  Toufic Kassouf, et al., bioRxiv - Cell Biology 2023

  Quote: ... following UbiScan protocols and instructions (Cell Signaling Technology) using Ubiquitin Branch Motif Antibody (K-ε-GG ...
• Atheroprone shear stress stimulates noxious endothelial extracellular vesicle uptake by MCAM and PECAM-1 cell adhesion molecules

  Pierre-Michaël Coly, et al., bioRxiv - Cell Biology 2023

  Quote: ... α6 integrin (Cell Signaling Technology, 3750), β1 integrin (Abcam ...
• Mechanically induced topological transition of spectrin regulates its distribution in the mammalian cortex

  Andrea Ghisleni, et al., bioRxiv - Cell Biology 2023

  Quote: ... and rabbit anti-β-actin (1:100, Cell Signaling). Before fixation ...
• Non-catalytic role of phosphoinositide 3-kinase in mesenchymal cell migration through non-canonical induction of p85β/AP-2-mediated endocytosis

  Hideaki T. Matsubayashi, et al., bioRxiv - Cell Biology 2023

  Quote: ... and phospho-FAK (Y397) (8556S) antibodies were purchased from Cell signaling. GAPDH antibody (sc-32233 ...
• Non-catalytic role of phosphoinositide 3-kinase in mesenchymal cell migration through non-canonical induction of p85β/AP-2-mediated endocytosis

  Hideaki T. Matsubayashi, et al., bioRxiv - Cell Biology 2023

  Quote: ... The reaction was stopped by directly replacing the culture media with 100 µL ice-cold RIPA buffer (Cell Signaling, 9806S) supplemented with cOmplete protease inhibitor (1× ...
• EDA2R/NIK signaling promotes skeletal muscle atrophy linked to cancer cachexia

  Sevval Nur Bilgic, et al., bioRxiv - Cancer Biology 2023

  Quote: ... TBS-T containing 5% milk was used (Cell signaling anti-rabbit (7074), anti-mouse (7076)) ...
• EDA2R/NIK signaling promotes skeletal muscle atrophy linked to cancer cachexia

  Sevval Nur Bilgic, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Nitrocellulose membrane was blotted with primary antibodies in TBS containing 0.05% Tween and 5% BSA (Cell signaling; p65-RelA (8242), phospho-p65-RelA-Ser536 (3033) ...
• MIIP downregulation promotes colorectal cancer progression via inducing adjacent adipocytes browning

  Qinhao Wang, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Protein lysates containing 1 mg total protein were precleaned and precipitated with indicated antibody or IgG (Cell Signaling Technology, Danvers, MA, US) for 6 h at 4°C ...
• MIIP downregulation promotes colorectal cancer progression via inducing adjacent adipocytes browning

  Qinhao Wang, et al., bioRxiv - Cancer Biology 2023

  Quote: ... anti-α-tubulin (#2144) and anti-GAPDH (#5174) were purchased from Cell Signaling Technology (Danvers, MA, US); anti-CD36 (ab252923) ...
• Chemo-sEVs release in cisplatin-resistance ovarian cancer cells are regulated by the lysosomal function

  Cristóbal Cerda-Troncoso, et al., bioRxiv - Cancer Biology 2023

  Quote: ... anti-RAB35 (cat# 9690) from Cell Signaling Technology, Danvers ...
• An Innovative Non-Hormonal Strategy Targeting Redox Active Metals to Down-Regulate Estrogen-, Progesterone-, Androgen- and Prolactin-Receptors in Breast Cancer

  Faten Shehadeh-Tout, et al., bioRxiv - Cancer Biology 2023

  Quote: ... The primary antibodies included the following from Cell Signaling Technology, MA ...
• Transmembrane protein CD69 acts as an S1PR1 agonist

  Hongwen Chen, et al., bioRxiv - Biophysics 2023

  Quote: ... Anti-mouse IgG HRP-linked secondary antibody (Cell Signaling Cat# 7076) was used for chemiluminescent detection (1:3,000 dilution).
• CRISPR/Cas9-Based Edition of Frataxin Gene in Dictyostelium discoideum for Friedreich’s Ataxia Disease Modeling

  Hernan G. Gentili, et al., bioRxiv - Cell Biology 2023

  Quote: ... an anti-FLAG monoclonal antibody (Cell Signaling Technology, [9A3] 8146S) was used followed by a secondary HRP-conjugated anti-mouse (ThermoFisher Cat ...
• Intermediate filament dysregulation and astrocytopathy in the human disease model of KLHL16 mutation in giant axonal neuropathy (GAN)

  Rachel Battaglia, et al., bioRxiv - Cell Biology 2023

  Quote: ... rabbit anti-Vimentin (Cell Signaling Technology, D21H3, IF 1:100), mouse anti-Vimentin (Thermo Fisher Scientific ...
• The Rho GAP RRC-1 is required for the assembly or stability of integrin adhesion complexes and is a member of the PIX pathway in muscle

  Jasmine C. Moody, Hiroshi Qadota, Guy M. Benian, bioRxiv - Cell Biology 2023

  Quote: ... and anti-HA (rabbit monoclonal C29F4 from Cell Signaling Technology). Secondary antibodies ...
• The Rho GAP RRC-1 is required for the assembly or stability of integrin adhesion complexes and is a member of the PIX pathway in muscle

  Jasmine C. Moody, Hiroshi Qadota, Guy M. Benian, bioRxiv - Cell Biology 2023

  Quote: ... anti-HA (rabbit monoclonal cat. no. C29F4 from Cell Signaling Technology) at 1:1,000 dilution ...
• The Rho GAP RRC-1 is required for the assembly or stability of integrin adhesion complexes and is a member of the PIX pathway in muscle

  Jasmine C. Moody, Hiroshi Qadota, Guy M. Benian, bioRxiv - Cell Biology 2023

  Quote: ... anti- HA (rabbit monoclonal antibody, cat. no. 3724 from Cell Signaling, Inc., at 1:1,000 dilution), anti-PIX-1 (rabbit polyclonal antibody (Moody et al. ...
• Unexpected inhibition of the lipid kinase PIKfyve reveals an epistatic role for p38 MAPKs in endolysosomal fission and volume control

  Daric J. Wible, et al., bioRxiv - Cell Biology 2023

  Quote: ... phospho-MK2 (pThr-222; #3316) and phospho-hsp27 (pSer-82; #2406) were purchased from Cell Signaling Technology (Danvers, MA). Anti-PI(3,5)P2 (Z-P035 ...
• Targeting the cell and non-cell autonomous regulation of 47S synthesis by GCN2 in colon cancer

  Marie Piecyk, et al., bioRxiv - Cancer Biology 2023

  Quote: ... from Cell Signaling Technology (Danvers, Massachussets, USA): Tubulin (2146) ...