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• T-cell derived extracellular vesicles prime macrophages for improved STING based cancer immunotherapy

  Aida S. Hansen, et al., bioRxiv - Immunology 2023

  Quote: ... anti-IkBa (Cell Signaling, L35A5), anti-CD9 (Santa Cruz ...
• T-cell derived extracellular vesicles prime macrophages for improved STING based cancer immunotherapy

  Aida S. Hansen, et al., bioRxiv - Immunology 2023

  Quote: ... anti-P65 (Cell Signaling, D14E12), anti-IkBa (Cell Signaling ...
• T-cell derived extracellular vesicles prime macrophages for improved STING based cancer immunotherapy

  Aida S. Hansen, et al., bioRxiv - Immunology 2023

  Quote: ... anti-STING (Cell Signaling, D2P2F), anti-vinculin (Sigma Aldrich ...
• T-cell derived extracellular vesicles prime macrophages for improved STING based cancer immunotherapy

  Aida S. Hansen, et al., bioRxiv - Immunology 2023

  Quote: ... anti-phospho-STING (Cell Signaling, D7C3S), anti-phospho-TBK1 (Cell Signaling ...
• T-cell derived extracellular vesicles prime macrophages for improved STING based cancer immunotherapy

  Aida S. Hansen, et al., bioRxiv - Immunology 2023

  Quote: ... anti-phospho-IRF3 (Cell Signaling, D601M), anti-IRF3 (Cell Signaling ...
• Bacterial meningitis in the early postnatal mouse studied at single-cell resolution

  Jie Wang, Amir Rattner, Jeremy Nathans, bioRxiv - Immunology 2023

  Quote: ... mouse anti-GAPDH (Cell Signaling Technologies 97166S); rabbit anti-GAPDH (Cell Signaling Technologies 5174S) ...
• Bacterial meningitis in the early postnatal mouse studied at single-cell resolution

  Jie Wang, Amir Rattner, Jeremy Nathans, bioRxiv - Immunology 2023

  Quote: ... Alexa Fluor 647 conjugate (Cell Signaling Technologies 14022); rabbit anti-Occludin (Invitrogen 406100) ...
• N-glycosylation engineering in chimeric antigen receptor T cells enhances anti-tumor activity

  Elien De Bousser, et al., bioRxiv - Immunology 2023

  Quote: ... or monoclonal rabbit anti-galectin-3 (1:200, Cell Signaling, 87985S) diluted in 1% w/v goat serum in PBS + 0.5% BSA + 0.1% Tween20 were incubated at overnight at 4°C ...
• Loss of genital epithelial barrier function is greater with depot-medroxyprogesterone acetate than intravaginal rings that release etonogestrel and ethinyl estradiol

  Mohan Liu, et al., bioRxiv - Immunology 2023

  Quote: ... incubated with SignalStain® Boost Detection and SignalStain® BAD Chromogen (both Cell Signaling Technology®), counterstained with hematoxylin ...
• Loss of genital epithelial barrier function is greater with depot-medroxyprogesterone acetate than intravaginal rings that release etonogestrel and ethinyl estradiol

  Mohan Liu, et al., bioRxiv - Immunology 2023

  Quote: ... MA) diluted 1:100 with SignalStain® antibody diluent (Cell Signaling Technology®). Sections were washed ...
• Gestational diabetes mellitus dysregulates the PD-1/PD-L1 axis at the feto-maternal interface

  ZN. Mihalic, et al., bioRxiv - Immunology 2023

  Quote: ... Anti-PD-L1 (clone E1L3N, 1:1000 dilution, Cell Signaling) and anti-GAPDH (clone 14C10 ...
• Gestational diabetes mellitus dysregulates the PD-1/PD-L1 axis at the feto-maternal interface

  ZN. Mihalic, et al., bioRxiv - Immunology 2023

  Quote: ... 1 mM CaCl2 in H2O) supplemented with 1:100 protease/phosphatase inhibitor (Cell Signaling). The protein content was measured using a Pierce BCA assay kit (Thermo Scientific) ...
• Gestational diabetes mellitus dysregulates the PD-1/PD-L1 axis at the feto-maternal interface

  ZN. Mihalic, et al., bioRxiv - Immunology 2023

  Quote: ... and anti-GAPDH (clone 14C10, 1:500 dilution, Cell Signaling) monoclonal antibodies were used ...
• Loss of genital epithelial barrier function is greater with depot-medroxyprogesterone acetate than intravaginal rings that release etonogestrel and ethinyl estradiol

  Mohan Liu, et al., bioRxiv - Immunology 2023

  Quote: ... Endogenous peroxidase activity was quenched by 3% hydrogen peroxidase prior to 4-hour incubation at 4°C with 5% normal goat serum (Cell Signaling Technology®, Danvers, MA) and rabbit anti-DSG1 (clone EPR677766(B) ...
• Mitochondrial translocation of TFEB regulates complex I and inflammation

  Chiara Calabrese, et al., bioRxiv - Cell Biology 2023

  Quote: ... Isotype specific rabbit IgG (2729, Cell Signaling Technology) was used as negative control ...
• Cytosolic retention of the mitochondrial protease HtrA2 during mitochondrial protein import stress (MPIS) triggers the DELE1-HRI pathway

  Paul Y. Bi, et al., bioRxiv - Cell Biology 2023

  Quote: ... GAPDH (5174S, Cell Signaling, 1/10000), HtrA2 (#9745 ...
• Cytosolic retention of the mitochondrial protease HtrA2 during mitochondrial protein import stress (MPIS) triggers the DELE1-HRI pathway

  Paul Y. Bi, et al., bioRxiv - Cell Biology 2023

  Quote: ... TOMM20 (42406, Cell Signaling, 1:2000), TOM40 (18409-1-AP ...
• Cytosolic retention of the mitochondrial protease HtrA2 during mitochondrial protein import stress (MPIS) triggers the DELE1-HRI pathway

  Paul Y. Bi, et al., bioRxiv - Cell Biology 2023

  Quote: ... HtrA2 (#9745, Cell Signaling, 1/1000), OMA1 (HPA055120 ...
• Cytosolic retention of the mitochondrial protease HtrA2 during mitochondrial protein import stress (MPIS) triggers the DELE1-HRI pathway

  Paul Y. Bi, et al., bioRxiv - Cell Biology 2023

  Quote: ... FLAG Tag (14793S, Cell Signaling, 1/1000), HRP Rabbit (111-035-003 ...
• Cytosolic retention of the mitochondrial protease HtrA2 during mitochondrial protein import stress (MPIS) triggers the DELE1-HRI pathway

  Paul Y. Bi, et al., bioRxiv - Cell Biology 2023

  Quote: ... LC3B (3868S, Cell Signaling, 1/1000 ...
• Cytosolic retention of the mitochondrial protease HtrA2 during mitochondrial protein import stress (MPIS) triggers the DELE1-HRI pathway

  Paul Y. Bi, et al., bioRxiv - Cell Biology 2023

  Quote: ATF4 (11815S, Cell Signaling, 1/2000), DELE1(PA5-57712 ...
• Caudal Fgfr1 disruption produces localised spinal mis-patterning and a terminal myelocystocele-like phenotype in mice

  Eirini Maniou, et al., bioRxiv - Developmental Biology 2023

  Quote: ... Primary antibodies were used in 1:100 dilution and were as follows: rabbit E-cadherin (3195, Cell Signaling Technology), mouse N-cadherin (14215S ...
• Caudal Fgfr1 disruption produces localised spinal mis-patterning and a terminal myelocystocele-like phenotype in mice

  Eirini Maniou, et al., bioRxiv - Developmental Biology 2023

  Quote: ... mouse N-cadherin (14215S, Cell Signaling Technology), rabbit pERK (#9101 ...
• TET1 Catalytic Activity is Required for Reprogramming of Imprinting Control Regions and Patterning of Sperm-Specific Hypomethylated Regions

  Rexxi D. Prasasya, et al., bioRxiv - Developmental Biology 2023

  Quote: Testes were homogenized by mortar and pestle in 1x RIPA buffer (Cell Signaling Technology) supplemented with Complete Protease Inhibitor (Roche) ...
• TET1 Catalytic Activity is Required for Reprogramming of Imprinting Control Regions and Patterning of Sperm-Specific Hypomethylated Regions

  Rexxi D. Prasasya, et al., bioRxiv - Developmental Biology 2023

  Quote: ... Cells were resuspended with H3K4me3 (Cell Signaling Technology 9751) or rabbit IgG antibodies (Cell Signaling Technology 2729 ...
• Integrating lipid metabolism, pheromone production and perception by Fruitless and Hepatocyte nuclear factor 4

  Jie Sun, et al., bioRxiv - Developmental Biology 2023

  Quote: ... anti-HA (Cell Signaling Technology, #3724S, 1: 500), anti-HNF4 guinea pig polyclonal antibody (1:100 ...
• The pluripotency factor Tex10 finetunes Wnt signaling for PGC and male germline development

  Dan Li, et al., bioRxiv - Developmental Biology 2023

  Quote: ... Cells were then washed with PBST and resuspended in MACS buffer with the primary antibody (Prdm1, Cell Signaling, 9115S) for 1.5 hours at 4 °C in the dark ...
• Catalytic antibodies in arrhythmogenic cardiomyopathy patients cleave desmoglein 2 and N-cadherin and impair cardiomyocyte cohesion

  Sunil Yeruva, et al., bioRxiv - Cell Biology 2023

  Quote: ... the wells were incubated with either goat anti-mouse IgG-HRP (Cell signaling #91196) at 1:2000 dilution in blocking buffer for DSG2-Abcam antibody ...
• FDX1 regulates cellular protein lipoylation through direct binding to LIAS

  Margaret B. Dreishpoon, et al., bioRxiv - Cell Biology 2023

  Quote: ... Actin (Cell signaling 8H10D10), ACO-2 (abcam ab129069) ...
• Kif6 regulates cilia motility and polarity in brain ependymal cells

  Maki Takagishi, et al., bioRxiv - Developmental Biology 2023

  Quote: ... Detection was carried out using HRP-linked anti-Rabbit IgG (1:2000, Cell signaling Technology, #7074) and visualized using Pierce ECL substrate (Thermo Fisher).
• Generation of human cerebral organoids with a structured outer subventricular zone

  Ryan Walsh, et al., bioRxiv - Developmental Biology 2023

  Quote: ... Primary antibodies were detected using the secondary anti-rat IgG HRp-linked (Cell Signaling, 7077S, 1:1000) together with the SuperSignal™ West Femto Chemiluminescent Substrate (Thermo Fischer Scientific).
• Kif6 regulates cilia motility and polarity in brain ependymal cells

  Maki Takagishi, et al., bioRxiv - Developmental Biology 2023

  Quote: ... or anti-GAPDH (1:1000, Cell Signaling Technology, #2118) antibodies ...
• Mitochondrial metabolism in Drosophila macrophage-like cells regulates body growth via modulation of cytokine and insulin signaling

  Shrivani Sriskanthadevan-Pirahas, et al., bioRxiv - Developmental Biology 2023

  Quote: ... The primary antibodies used in this study were: anti-phospho-AKT-Ser505 (1:1000, Cell Signaling Technology #4054), anti-actin (1:1000 ...
• Mitochondrial metabolism in Drosophila macrophage-like cells regulates body growth via modulation of cytokine and insulin signaling

  Shrivani Sriskanthadevan-Pirahas, et al., bioRxiv - Developmental Biology 2023

  Quote: ... and anti-phospho-JNK (1:500, Cell Signaling Technolog #4668S). Goat and donkey secondary antibodies were purchased from Santa Cruz Biotechnology (sc-2030 ...
• Coordination between endoderm progression and gastruloid elongation controls endodermal morphotype choice

  Naama Farag, Chen Schiff, Iftach Nachman, bioRxiv - Developmental Biology 2023

  Quote: ... Antibodies used: E-cadherin (Cell Signaling 24E10), Alexa405 (secondary).
• TET1 Catalytic Activity is Required for Reprogramming of Imprinting Control Regions and Patterning of Sperm-Specific Hypomethylated Regions

  Rexxi D. Prasasya, et al., bioRxiv - Developmental Biology 2023

  Quote: ... membranes were probed with 1:10,000 rabbit anti-GAPDH (2118, Cell Signaling Technology) overnight at 4°C ...
• The pluripotency factor Tex10 finetunes Wnt signaling for PGC and male germline development

  Dan Li, et al., bioRxiv - Developmental Biology 2023

  Quote: ... or normal rabbit IgG (6 μg per ChIP, Cell Signaling Tech., 2729P) as a negative control was incubated with digested chromatin overnight with rotation at 4 °C and then incubated with 30 μl Protein G magnetic beads for 2 h at 4 °C with rotation ...
• Integrating lipid metabolism, pheromone production and perception by Fruitless and Hepatocyte nuclear factor 4

  Jie Sun, et al., bioRxiv - Developmental Biology 2023

  Quote: ... The following antibodies were used in immunostaining: anti-GFP (Cell Signaling Technology, #2956S, 1:200), anti-HA (Cell Signaling Technology ...
• Ultrafast and long-range coordination of wound responses is essential for whole-body regeneration

  Yuhang Fan, et al., bioRxiv - Developmental Biology 2023

  Quote: ... pH 7.4 in MillQ H2O) supplemented with equal volumes of DMSO or 25 µM U0126 (Cell Signaling) in DMSO for the control and Erk-inhibited groups respectively ...
• TET1 Catalytic Activity is Required for Reprogramming of Imprinting Control Regions and Patterning of Sperm-Specific Hypomethylated Regions

  Rexxi D. Prasasya, et al., bioRxiv - Developmental Biology 2023

  Quote: ... or rabbit IgG antibodies (Cell Signaling Technology 2729) at a final dilution of 1:100 in 200 μL of antibody binding buffer (same as Wash buffer with 0.05% digitonin and 2 mM EDTA ...
• Pancreatic Deletion of Mitogen-inducible Gene 6 Promotes Beta Cell Proliferation Following Destruction

  Kimberley El, et al., bioRxiv - Cell Biology 2023

  Quote: ... Phosphorylated EGFRY1068 (Cell Signaling, #3777 ...
• Fgf8 regulates first pharyngeal arch segmentation through pouch-cleft interactions

  Nathaniel Zbasnik, Jennifer L. Fish, bioRxiv - Developmental Biology 2023

  Quote: ... The mitotic marker Phospho-Histone H3/ Ser10 (Cell Signaling 9701, 1:100 rabbit) was used to label proliferating cells ...
• Six3 acts independently of Pax6 to provide an essential contribution to lens development

  Sumanth Manohar, et al., bioRxiv - Developmental Biology 2023

  Quote: ... Blocking solution was replaced with 1° antibody (Ab) (Phospho-Smad1/5/9 Rabbit mAb #13820, Cell Signaling Technology 13820T, 1:300) in 10%NGS/PBS-Tx and the slides were incubated overnight at 4°C ...
• The multimodal action of G alpha q in coordinating growth and homeostasis in the Drosophila wing imaginal disc

  Vijay Velagala, et al., bioRxiv - Developmental Biology 2023

  Quote: ... and DCP-1 (#9578S; Cell Signaling), diluted to 1:100 in 5% NGS ...
• The neurodevelopmental transcriptome of the Drosophila melanogaster microcephaly gene abnormal spindle reveals a role for temporal transcription factors and the immune system in regulating brain size

  Maria C. Mannino, et al., bioRxiv - Developmental Biology 2023

  Quote: ... anti-cleaved Death Caspase-1 (DCP-1, 1:100, Cell Signaling Technology), anti-β-Tubulin (1:250 ...
• Follicle stimulating hormone signaling opposes the DRL-1/FLR-4 MAP Kinases to balance p38-mediated growth and lipid homeostasis in C. elegans

  Sarah K. Torzone, et al., bioRxiv - Developmental Biology 2023

  Quote: ... and probed with either anti-phospho-p38 MAPK (Thr180/Tyr182; #9211, Cell Signaling Technology), anti-PMK-1 (Peterson et al ...
• The multimodal action of G alpha q in coordinating growth and homeostasis in the Drosophila wing imaginal disc

  Vijay Velagala, et al., bioRxiv - Developmental Biology 2023

  Quote: ... The following primary antibodies were used: PH3 (#9701S, Cell Signaling), diluted to 1:800 in 5% NGS ...
• Endothelial SMAD1/5 signaling couples angiogenesis to osteogenesis during long bone growth

  Annemarie Lang, et al., bioRxiv - Developmental Biology 2023

  Quote: ... The following primary antibodies and secondary antibodies were used (staining durations are individually provided): pSMAD1/5 (Cell signaling; clone ...
• C. elegans SMOC-1 interacts with both BMP and glypican to regulate BMP signaling

  Melisa S. DeGroot, et al., bioRxiv - Developmental Biology 2023

  Quote: ... rabbit anti-V5 IgG monoclonal antibody (Cell Signaling, D3H8Q, diluted 1:1000), mouse anti-actin IgM JLA20 monoclonal antibody (Developmental Studies Hybridoma Bank ...
• C. elegans SMOC-1 interacts with both BMP and glypican to regulate BMP signaling

  Melisa S. DeGroot, et al., bioRxiv - Developmental Biology 2023

  Quote: ... rabbit anti-HA IgG monoclonal antibody (Cell Signaling, C29F4, diluted 1:1000), mouse anti-V5 IgG monoclonal antibody (Invitrogen (E10/V4RR) ...