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Citations for Cell Signaling Technology :
651 - 700 of 715 citations for Sulfur Free Cobalt Metallo Organic Standard Co @ 5000 µg g in Hydrocarbon Oil since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Samples containing 10 µg of DNA were immunoprecipitated with 375 ng (10 µl) of TCF7L2 antibody (Cat. no. 2569, Cell Signaling). Incubation with protein G agarose beads and elution of precipitated chromatin was conducted according to ChIP-IT High Sensitivity kit protocol (Cat ...
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bioRxiv - Cancer Biology 2022Quote: ... The chromatin was then incubated with 5 µg of antibody against KDM6A (A302-374A, Bethyl Laboratories and 33510S, Cell Signaling Technology), KDM6B (ab169197 ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 µg/mL of recombinant human IgG1 Fc or Siglec-11 Fc chimera protein was precomplexed with 0.5 µg/mL of Protein A -HRP (Cell Signaling Technology) on ice for 30 minutes ...
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bioRxiv - Physiology 2024Quote: ... Triton X-100 CD31+ and CD31-cell lysates (5-15 µg/lane) were analyzed by western blot for proteins and eNOS (#32027; 1:500; Cell Signaling) and GAPDH (#97166 ...
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bioRxiv - Molecular Biology 2024Quote: ... 150 to 250 µg of chromatin were incubated overnight on a rotator at 4°C with Ezh2 antibody (Cell Signaling Technology). On the next day ...
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bioRxiv - Neuroscience 2024Quote: ... Free floating slices were stained for SA-β-gal per the manufacturer’s protocol (Cell Signaling Technology Danvers, MA, Cat#9860). Each slice was rinsed with 1X PBS then transferred to 1X Fixative Solution for 10-15 minutes followed by rinsing twice with 1X PBS ...
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bioRxiv - Immunology 2020Quote: ... The same amount of protein was immunoprecipitated with anti-NEMO and ChIP-Grade Protein G Magnetic Beads (Cell Signaling Technology). The IKKα/β kinase activity in the IP complex was tested by using IKKβ Kinase Enzyme Kit (Promega ...
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Targeting adipocyte ESRRA promotes osteogenesis and vascular formation in adipocyte-rich bone marrowbioRxiv - Molecular Biology 2023Quote: ... Chromatin complexes were immunoprecipitated with primary antibody as below and incubated with protein G magnetic beads (Cell Signaling Technology #9006) overnight ...
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bioRxiv - Biochemistry 2024Quote: ... that combines the IgG binding domain of both Protein A and Protein G were used to cross-link anti-Kv11.1 antibodies (Cell Signaling). Stock beads with a concentration of 10 mg/ml were washed four times with 10 volumes of Dulbecco’s Phosphate Buffered Saline (DPBS ...
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bioRxiv - Immunology 2023Quote: ... cells were washed twice with Buffer 1 and resuspended in 50ul of Buffer 1 + 1X pA/G-MNase (Cell Signaling) and incubated on ice for 1 hr and washed twice with Buffer 2 (0.05% w/v Saponin ...
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bioRxiv - Cancer Biology 2023Quote: ... Up to 30 µg of protein lysate was separated by SDS-PAGE and transferred to nitrocellulose membranes before antibody incubation (IκBα: Cell Signaling, CS9242 ...
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bioRxiv - Microbiology 2021Quote: ... 24 h later cells were treated with increasing concentrations (20, 40, 80 µg/ml) of α-IFITM2 (Cell Signaling, Cat#13530 S) or Remdesivir (Selleck Chemicals Cat#S8932 ...
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bioRxiv - Neuroscience 2020Quote: ... 20 µg of total brain protein were loaded and anti-GFAP antibody (D1F4Q) XP Rabbit mAb (1:3000; Cell Signaling Technology, 12389), anti-Iba1 antibody (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... and 10 µg/mL of aprotinin and leupeptin] and incubated (16 hrs., 4°C) with either 3 µg non-immune control rabbit IgG (Cell Signaling #272) or with 3 µg of ERRα antibody (Cell Signaling #13826 ...
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bioRxiv - Plant Biology 2024Quote: ... Twenty µl was saved as input and the remaining supernatant was then incubated at 4°C overnight with specific antibodies (5 µg of H3K27me3 Ab, Diagenode C15410069 or Millipore 07-449; 10 µl H2AUb Ab, Cell Signaling Technology ref.8240 ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins samples were processed in SDS free lysis buffer for the assessment of Ras-GTP level using Active Ras Detection Kit (Cell Signaling) by following manufacturer’s instructions.
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bioRxiv - Developmental Biology 2020Quote: ... Cells were then centrifuged again and removed of supernatant before resuspension in isolation buffer (calcium and magnesium-free 1xPBS, 0.1% (w/v) bovine serum albumin (BSA) (Cell Signaling Technology), 2mM EDTA ...
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bioRxiv - Genetics 2021Quote: ... the samples were incubated with 30 ml of ChIP Grade Protein G magnetic beads (Cat# 9006S; Cell Signaling Technology; Danvers, MA) for 2 hours at 4°C under gentle rotation ...
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bioRxiv - Biochemistry 2020Quote: ... Bound primary antibodies were detected with HRP-conjugated goat anti-mouse immunoglobulin-G at 1:5,000 (Cell Signaling Technoloy, Cat# 7076S) or goat anti-rabbit immunoglobulin-G at 1:2,500 (Cell Signaling Technology ...
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bioRxiv - Molecular Biology 2022Quote: ... For immunoprecipitation assays, cell lysates were incubated with Protein G Agarose (cat # 20398, Pierce) and anti-Flag antibody (cat # 2368, Cell Signaling) at 4°C overnight ...
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bioRxiv - Microbiology 2020Quote: ... Lysed samples were centrifuged and incubated for 3 h with Pierce Protein A/G Magnetic beads (88802) which were pre-incubated over night with V5 antibody (Cell signaling E9H80 ...
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bioRxiv - Pathology 2022Quote: ... Then membranes were incubated with peroxidase-conjugated secondary antibodies for 1 h at room temperature: anti-rabbit immunoglobulin G (IgG) (AB_2099233; Cat.#7074, 1:2000, Cell Signaling Technology), anti-mouse IgG (AB_330924 ...
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bioRxiv - Molecular Biology 2022Quote: ... anti-rabbit immunoglobulin G (IgG) (H + L) (Dylight 800 conjugates) and anti-mouse IgG (H + L) (Dylight 680 conjugates) (Cell Signaling) were used as secondary antibodies.
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bioRxiv - Molecular Biology 2021Quote: ... Individual ChIP experiments were performed on pre-cleared chromatin using antibody-coupled ChIP grade Protein G magnetic beads (Cell Signaling Technology). POLR3A antibody was obtained from Abcam (ab96328 lot#GR318563) ...
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bioRxiv - Molecular Biology 2023Quote: ... washed twice in PBS and after centrifugation at 250 x g for 5 min at 4°C resuspended in cell lysing buffer (Cell Signaling Cat# 9803 ...
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bioRxiv - Cancer Biology 2022Quote: ... Histone modification ChIPs were performed by using 10 µg of chromatin each incubated with the recommended amount of H3K27Ac (Cell Signaling #8173, 5ul), H3K4me1 (Cell Signaling #5326 ...
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bioRxiv - Genomics 2024Quote: ... with primary antibody (0.25 µL polyclonal rabbit αHP1 [11]; 0.5 µg αH3K9me3, Abcam 8988; 0.5 µL αBiotin (Cell Signaling Technology # D5A7; for DiBioCUT&Tag) or 0.5 µg normal rabbit IgG (MERCK ...
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bioRxiv - Neuroscience 2024Quote: ... the 96-well MSD plates (Meso Scale Diagnostics, L15XA) were coated with 1 µg/mL p-S65-Ub capturing antibody (Cell Signaling Technology, 62802), blocked with 1% BSA in TBST ...
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bioRxiv - Cell Biology 2024Quote: ... Equal amount around 20 µg total protein from the lysate was incubated against anti-p300 CBP HAT antibody (Cell Signaling Technology, Cat #: 86377) respectively at 4°C for 90 min ...
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bioRxiv - Genomics 2023Quote: ... blocked in TBST buffer supplemented with 5% bovine serum albumin (BSA) or 5% fat-free milk and incubated overnight with primary anti-LEF1 antibody (Cell Signaling, #2230), anti-p16 antibody (ThermoFisher ...
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bioRxiv - Neuroscience 2024Quote: ... Immunofluorescence staining was conducted on free-floating slices using a rabbit monoclonal anti c-fos (1:1000, Cell Signaling Technology, cat# 2250s) and mouse monoclonal anti-GAD67 (1:500 ...
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bioRxiv - Cell Biology 2024Quote: ... blocked in TBST buffer supplemented with 5% bovine serum albumin (BSA) or 5% fat-free milk and incubated overnight with primary antibody anti-PERK (Cell signaling, #3192), anti-phospho-PERK (Thr908 ...
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bioRxiv - Neuroscience 2021Quote: ... Immunoprecipitation was performed with 2 µg rabbit polyclonal anti-KDM2B (gift from Dr. Robert Klose, Oxford University) and rabbit anti-RING1B antibody (1:100, Cell Signaling Technology) overnight at 4°C ...
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bioRxiv - Plant Biology 2020Quote: ... Immuno-precipitation was performed for two replicates for each genotype (about 1 g tissue/IP) by SimpleChIP Plus Kit (Cell Signaling Technology) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Ten to 50 μg of proteins were separated by 10 % SDS-PAGE and visualized with primary Slug or E-cadherin antibody (C19G7, Cell Signaling Technology, #9585 ...
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bioRxiv - Immunology 2020Quote: ... The cell lysates were incubated overnight at 4 °C with NEMO antibody and ChIP-Grade Protein G Magnetic Beads (Cell Signaling Technology).
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bioRxiv - Molecular Biology 2023Quote: ... and myc- and HA-tagged proteins were immunoprecipitated with Protein A/G beads (GeneBio, 22202B-1) coated with anti-myc antibody (Cell signaling, 2276S) at a dilution of 1:250 or anti-HA antibody (Cell signaling ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cells (2 x106/sample) were collected by centrifugation at 300 g for 4 min and resuspended in cell lysis buffer (Cell Signaling #9803) supplemented with protease inhibitors (Sigma #5056489001) ...
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bioRxiv - Neuroscience 2024Quote: ... sheared chromatin was centrifuged at 16,000 x g for 20 minutes at 4 °C and dissolved in 1x ChIP buffer (Cell Signaling Technology, #57976). 2% input was taken and chromatin was incubated with antibodies overnight at 4°C ...
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bioRxiv - Genomics 2024Quote: ... Cell lysate was centrifuged at 21,000 x g for 10 minutes and supernatant was mixed with Blue Loading Buffer (Cell Signaling Technology #7722) per the manufacturer’s recommended ratio ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting mixtures of protein-DNA and antibody were further incubated with ChIP-Grade Protein G Magnetic Beads (9006, Cell Signaling Technology). After sequential washing with low- and high-salt solutions ...
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bioRxiv - Neuroscience 2022Quote: ... The remaining 90 μg of each sample was pooled for phospho-peptide enrichment with PTMScan Phospho-Enrichment IMAC FE-NTA Magnetic Beads (Cell Signaling Technology) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... the resulting supernatants were incubated for 3 hours at 4°C with a 2.5 µL slurry of Sera-Mag SpeedBeads Protein A/G (Cytiva) pre-incubated with 2.5 µL of anti-HA-tag rabbit mAb (Cell signaling technology, C29F4). The beads were washed four times with the lysis buffer and then twice with 50 mM ammonium bicarbonate ...
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bioRxiv - Cell Biology 2024Quote: ... Bis(sulfosuccinimidyl)suberate disodium salt (BS3; Dojindo, #B574) was used to crosslink the antibodies to protein G magnetic beads (Cell Signaling Technology). Benzonase (Merck ...
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bioRxiv - Plant Biology 2024Quote: ... overnight at 4 °C and then with horseradish peroxidase (HRP)-conjugated anti-mouse immunoglobulin G (IgG) antibody (7076S; Cell Signaling Technology, USA) for 1 hour at room temperature ...
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bioRxiv - Genetics 2024Quote: ... The clarified nuclear extracts were incubated with IgG antibody applied as negative control or target antibody shown in Table 2 with rotation overnight at 4°C and conjugated with Chromatin immunoprecipitation-grade protein G magnetic beads (Cell Signaling Technology). After extensive washing ...
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bioRxiv - Cancer Biology 2024Quote: ... 25 µL of blocked beads were coupled with 5 µg of Acetyl-Histone H3 (Lys27) (D5E4) XP® Rabbit mAb or Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb (Cell Signaling) or Mono-Methyl-Histone H3 (Lys4 ...
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bioRxiv - Developmental Biology 2022Quote: ... Pulling down of the pre-cleared chromatin was performed with the use of magnetic beads that were previously incubated with 5 µg of antibody (Histone H3 Lysine 4 trimethylation; H3K4me3; Cell Signaling Technology, cat. #9751) for 8 hours at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... Whole cell lysates (20 µg) were subjected to SDS-PAGE and immunoblotted with specific antibodies against pFRS2a (#3864; Cell signaling Technology, Beverly, MA, USA), FRS2 (sc-17841 ...
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bioRxiv - Neuroscience 2024Quote: ... The incubation was performed using the free-floating method at 4°C (48 h) with rabbit anti-p75NTR(1:500; Cell signaling, MA, USA) with 0.6% Triton X-100 in PBS with 5% BSA ...