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Citations for Cell Signaling Technology :
551 - 600 of 715 citations for Sulfur Free Cobalt Metallo Organic Standard Co @ 5000 µg g in Hydrocarbon Oil since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: ... membranes that had been washed three times 20 minutes in TBS with 0.1% Tween 20 at room temperature were incubated with HRP-conjugated goat anti-mouse antibody (Cell Signaling Technology; 1:5000). The chemiluminescent signal was developed using the Clarity Western ECL substrate (Bio-Rad ...
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bioRxiv - Biochemistry 2024Quote: ... 1 hour minimum), proteins were incubated with primary antibodies against GFP (Clonetech, 632381) at 1:5000 dilution and β-actin-HRP (Cell Signaling, 12261) at 1:1000 dilution for 1 hour at room temperature or overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... according to standard protocols and using antibodies directed against p53-Ser15 (clone 1C12) or total p53 (pAb) (Cell Signaling Technology). For quantification ...
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bioRxiv - Cell Biology 2021Quote: ... 20 µg of the total protein for p-STAT3 Tyr705 (#9131S; Cell Signaling Technology, RRID:AB_331586), α-tubulin (#3873 ...
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bioRxiv - Neuroscience 2024Quote: ... 300 µg cell lysates were incubated with anti-phospho-tyrosine antibody (#8954, Cell Signaling Technology) at 4 °C overnight ...
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bioRxiv - Neuroscience 2023Quote: ... anti-phospho-TBK1/NAK S172 (0.141 µg/ml, Cat. # 5483, RRID: AB_10693472; Cell Signaling Technology), anti-eIF4E (0.116 µg/ml ...
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bioRxiv - Bioengineering 2024Quote: ... and Alexa Fluor 647 (4 µg/mL, A21244, Invitrogen and 1:500, #4410, Cell Signaling). To stain for nucleus and cytoskeleton ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were washed twice with PBS-BSA and then co-stained for 20 minutes with anti-myc Alexa 647 (clone 9B11, 1/250 dilution; Cell Signaling Technology) and anti-HIS-FITC (chicken polyclonal ...
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bioRxiv - Physiology 2022Quote: ... The following day sections were washed in wash buffer 3 times for 5 min and co-stained for anti-Rabbit IgG Fab2 Alexa Fluor 488 (Cell Signaling, 4412S) at 1:1000 dilution at room temperature for 1 h ...
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bioRxiv - Cancer Biology 2023Quote: ... Beads were resuspended in 1ml total volume cold Co-IP Buffer containing rabbit IgG or rabbit anti-FLAG antibody (Cell Signaling #14793S), 4μg per sample ...
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bioRxiv - Biochemistry 2020Quote: ... or goat anti-rabbit immunoglobulin-G at 1:2,500 (Cell Signaling Technology, Cat# 7074S) by chemiluminescence (VWR ...
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bioRxiv - Evolutionary Biology 2021Quote: ... After the lysate was cleared with Protein A and G magnetic beads (Cell Signaling), 50 µl of the cleared sample was set aside as input DNA ...
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bioRxiv - Cell Biology 2022Quote: ... The lysates were then pre-cleared with equilibrated protein G magnetic beads (Cell Signaling) for 1h at RT with rotation ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... immunocomplexes were incubated with pre-washed Protein G Magnetic beads (#70024 from Cell Signaling) under agitation for 1 hour at room temperature ...
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bioRxiv - Synthetic Biology 2021Quote: ... membranes were probed with the following antibodies at 1/2500 or 1/5000 dilution: rabbit anti-p44/42 MAPK (ERK1/2) antibody (Cell Signaling, cat # 4695 S) to detect ERK2 ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was then washed 3 times with TBST and the secondary goat anti-rabbit IgG horseradish peroxidase (HRP)-conjugated antibody (1:5000; cat. no. 7074, Cell Signaling Technology, The Netherlands) was added and allowed to bind at room temperature for 1 hour ...
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IL-27R signaling serves as immunological checkpoint for NK cells to promote hepatocellular carcinomabioRxiv - Immunology 2020Quote: ... Each membrane was washed and primary antibodies (except β-actin-HRP) were detected with a 1:5000 dilution of HRP-conjugated rabbit anti-mouse IgG (Cell Signaling Technology, 7076S) or mouse anti-rabbit IgG (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2024Quote: ... we used the β-amyloid 1-37 (D2A6H) antibody from Cell Signaling Technology (carrier-free). Capture antibody was adsorbed to plates at 1mg/ml (empirically optimised ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were transferred to nitrocellulose using standard methods and membranes were blocked in 5% non-fat dry milk (Cell Signaling Technology) in TBS with 0.2% Tween-20 (Boston BioProducts) ...
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bioRxiv - Molecular Biology 2020Quote: ... Western blotting was performed according to standard procedures using the following antibodies and dilutions: H3K27me3 (1:1000 Cell Signaling C36B11, rabbit), H3K9me2 (1:1000 Upstate 07-441 ...
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bioRxiv - Physiology 2023Quote: ... Protein expression was measured by standard western blot procedures using anti-mouse primary antibodies against Arf6 (rabbit monoclonal; 1:1000; 19kDa; Cell Signaling), β-actin (mouse monoclonal ...
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bioRxiv - Cancer Biology 2023Quote: ... phospho-H2AX (S139) (20E3) rabbit mAb (1:400 for standard immunofluorescence, 1:100 for FFPE tissue staining, Cell Signaling Technology #9718); p21 (12D1 ...
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bioRxiv - Cancer Biology 2021Quote: ... Supernatants were incubated with 4 µg of Anti-EGFR IgG1 (D38B1) (Cell Signaling Technology, Danver, MA) and 20 µg of protein A beads (Santa Cruz ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Protein samples (30 µg) obtained from lysis in RIPA buffer (Cell Signaling Technology, Danvers, MA, USA) of infected cells were separated on 10% SDS-PAGE and then transferred onto polyvinylidene difluoride (PVDF ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was incubated with 5 µg of either the targeted antibodies (SRSF3: Cell Signaling Technology #35073 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... then resuspended in 300 µL of FACS buffer containing 0.2 µg/mL DAPI (Cell Signaling Technologies).
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bioRxiv - Genomics 2024Quote: ... Immunoprecipitation was performed using either 3 µg of either SMAD1 (SMAD1 XP, Cell Signaling Technology 6944), SMAD2/3 (SMAD 2/3 XP ...
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bioRxiv - Cell Biology 2024Quote: ... co-housed 6-month-old female C57BL/6J wildtype mice were administered either: 1) 6mg/kg of Necrostatin1s (Cell Signaling Technologies Cat#17802) in dimethyl sulfoxide via intraperitoneal injection then 15-30 minutes later given 150 μg/kg TNF (BioTimes Inc ...
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bioRxiv - Molecular Biology 2021Quote: ... Supernatants were precleared with 300uL ChIP-grade Protein G magnetic beads (Cell Signaling Cat. 9006) and then incubated rotating overnight at 4C with 10 µg anti-HA ChIP- grade antibody (Abcam ab9110) ...
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bioRxiv - Cell Biology 2021Quote: ... and ChIP grade Protein-G agarose beads were purchased from Cell Signaling Technology (Danvers, MA). Anti-TRPC6 (#PA5-20256) ...
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bioRxiv - Cell Biology 2021Quote: ... Immunocomplexes were captured by rotation with protein G-coupled magnetic beads (Cell Signaling Technologies, 9006) for 2 h at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... Sheared chromatins were incubated with Protein G Beads and non-immune IgG (Cell Signaling #2729), Polymerase II (Santa Cruz #sc56767) ...
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bioRxiv - Cancer Biology 2022Quote: ... The immunocomplexes were then conjugated with ChIP-Grade Protein G Magnetic Beads (Cell Signaling Technology) for 2 hours at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... or goat anti-rabbit immunoglobulin-G at 1:3000 dilution (Cell Signaling Technology, Cat# 7074S) respectively using the chemiluminescence reagent (VWR ...
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bioRxiv - Cancer Biology 2023Quote: ... Precipitation for all samples was performed with protein G coupled paramagnetic beads (Cell Signaling Technologies). Antibodies used for ChIP ...
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bioRxiv - Immunology 2023Quote: ... and resuspended in 50ul of Buffer 1 + 1X pA/G-MNase (Cell Signaling, cat. 57813) and incubated on ice for 1 hour and washed twice with Buffer 2 (0.05% w/v Saponin ...
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bioRxiv - Immunology 2024Quote: ... Antibodies were conjugated in lysate to protein G-coupled agarose beads (Cell Signaling Technology, 37478P). Glycine buffer elution was used following IP as per the linked protocol and eluate was processed as for western blot (note that the sample buffer used for blotting contains β-mercaptoethanol ...
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bioRxiv - Immunology 2024Quote: ... and resuspended in 50ul of Buffer 1 + 1X pA/G-MNase (Cell Signaling, cat. 57813) and incubated on ice for 1 hour and washed thrice with Buffer 2 (0.05% w/v Saponin ...
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bioRxiv - Cancer Biology 2020Quote: ... Primary antibodies against PRDM1 (1:750 dilution, gift from Dr. Philip W. Ingham)46 and total histone H3 (1:5000 dilution, Cell Signaling Technology, Danvers, MA, #9715) were used ...
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bioRxiv - Cancer Biology 2024Quote: ... membranes were washed twice with TBST and incubated for 1 hour with HRP-linked anti-rabbit IgG or anti-mouse IgG (Cell Signaling, 1:5000 – 1:10,000) at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... Blots were washed with TBST for 10 min three times before incubation with secondary antibodies [Anti-rabbit IgG-HRP (Cell Signaling Technology 7074S, 1:5000), Anti-mouse IgG- HRP (Cell Signaling Technology 7076S ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were fixed in 4% paraformaldehyde and processed for immunostaining by standard methods using mouse anti-Flag (9A3, Cell Signaling, 1:400), rabbit anti-Myc (71D10 ...
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bioRxiv - Cell Biology 2022Quote: Larval tissues were stained with standard immunohistochemical procedures using rabbit anti-Phospho-JNK polyclonal antibody (Cell Signaling Technology, Cat #4668, 1:100), chicken anti-β-galactosidase antibody (Abcam ...
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bioRxiv - Biochemistry 2022Quote: Concentrated stock of isotopically labelled internal standard (heavy) peptides (M1, M1ox, K6, K6ox, K11, K27, K29, K33, K48, K63) were purchased from Cell Signaling Technologies and Cambridge Research Biochemicals (Cleveland ...
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bioRxiv - Cell Biology 2021Quote: ... Immunohistochemistry was carried out via standard techniques with non-phospho (Active) β-Catenin (#8814, rabbit monoclonal, Cell Signaling Technology; diluted 1:200) and EGR2 (ab43020 ...
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bioRxiv - Neuroscience 2021Quote: ... Immunofluorescence staining was performed by a standard protocol using an anti-HA rabbit primary antibody (C29F4, Cell Signaling Technology, diluted 1:1600) and a secondary antibody goat anti-rabbit Alexa Fluor 633 (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... and immunoblotted for ubiquitin as per standard protocols (anti-ubiquitin antibody P4G7, BioLegend #838701, 1:1000; anti-mouse IgG HRP-linked antibody, Cell Signaling Technology #7076 ...
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bioRxiv - Cancer Biology 2023Quote: ... PD-L1 immunohistochemistry was performed on 4-μm FFPE tumor tissue sections using a standard PD-L1 antibody (E1L3N; dilution 1:100, Cell Signaling Technologies) validated in the clinical laboratory at the study institution ...
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bioRxiv - Developmental Biology 2024Quote: ... cells were fixed in 4% PFA for 10 minutes and processed for immunostaining by standard methods using combinations of rabbit (Cell Signaling, 71D10) or mouse anti-Myc (Cell Signaling ...
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bioRxiv - Pathology 2022Quote: ... tissues were incubated for 30 minutes in Animal free Blocking solution (Cell Signaling 15019S, 1:5) and then with primary antibodies at 4°C ON ...