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Citations for Cell Signaling Technology :
501 - 550 of 715 citations for Sulfur Free Cobalt Metallo Organic Standard Co @ 5000 µg g in Hydrocarbon Oil since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... The membrane was then transferred to a secondary antibody solution containing a 1:5000 dilution of α-mouse HRP (Cell Signaling) for one hour of shaking at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... and 1:5000 dilution of HRP-conjugated rabbit anti-mouse secondary antibody (Cat. No. 7076, Lot No. 36, Cell Signaling Technology), and the blot was developed similarly.
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bioRxiv - Cell Biology 2022Quote: ... The membrane was blocked with 5% non-fat dry milk in TBST and incubated with primary antibodies against Perilipin-2 (1:5000 gift from Dr. Ronal Kuhnlein) 13 and Actin (1:1000, Cell Signaling). The western blot images were captured by a BIO-RAD workstation.
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bioRxiv - Biochemistry 2023Quote: ... β-actin levels were detected using a rabbit anti-β-actin antibody at 1:5000 dilution (Cell Signaling Technology, Catalog# 4970S). Primary antibodies were detected with an HRP-conjugated anti-mouse immunoglobulin-G at 1:5000 dilution (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were then incubated with horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (1:5000, CS7076, Cell Signaling, Danvers, MA, USA) and goat anti-rabbit IgG (1:5000 ...
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bioRxiv - Cancer Biology 2024Quote: ... Secondary antibodies used were anti-rabbit and anti-mouse IgG HRP-linked antibodies (1:5000, Cell Signaling, 7074 and 7076 respectively). Images were developed using the MINI-MED 90 X-Ray Film Processor (AFP Manufacturing Corp) ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were then washed with TBS-T and incubated for 1 h at room temperature with peroxidase conjugated anti-mouse or anti-rabbit IgG (1:5000 dilution, 7076 and 7074, Cell Signaling). Blots were developed using Advanced Molecular Image Imager and ChemoStar Software ...
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bioRxiv - Biochemistry 2024Quote: ... β-actin levels were detected using a rabbit anti-β-actin antibody at 1:5000 dilution (Cell Signaling Technology, Catalog# 4970S). Primary antibodies were detected with an HRP-conjugated anti-mouse immunoglobulin-G at 1:5000 dilution (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2021Quote: ... and 30 μL ChIP-Grade Protein G Magnetic Beads (#9006, Cell Signaling) were added ...
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bioRxiv - Molecular Biology 2021Quote: ... Thirty μL magnetic protein G beads (SimpleChIP Kit #9003, Cell Signaling Technology) were added to the samples and incubated at 4°C with end-over-end rotation for 4 h ...
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bioRxiv - Molecular Biology 2023Quote: ... Then a 50% slurry of Protein G agarose beads (Cell Signaling, #37478) in RIPA lysis buffer was added ...
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bioRxiv - Molecular Biology 2022Quote: ... 30 μL of protein G Agarose Beads (Cell Signaling Technology, MA, USA) slurry was added to the protein mixture and rotated at 4 °C for 3 h ...
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bioRxiv - Cancer Biology 2024Quote: ... Immunoprecipitated products were isolated with Protein G Magnetic Beads (Cell Signaling Technology) and subjected to reverse cross-linking ...
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bioRxiv - Neuroscience 2020Quote: ... 1 µg of a rabbit monoclonal anti-p75NTR antibody (clone D4B3; Cell Signaling Technology) or mouse monoclonal anti-PSD-95 antibody (clone 7E3-1B8 ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 µg of anti-Matrin3 or normal rabbit IgG (Cell Signaling, Catalog no. 2729S) were coupled with the beads overnight ...
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bioRxiv - Neuroscience 2023Quote: ... anti-phospho-IRF3 S396 (0.046 µg/ml, Cat. # 4947, RRID: AB_823547; Cell Signaling Technology), anti-TBK1/NAK (0.19 µg/ml ...
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bioRxiv - Neuroscience 2023Quote: ... or anti-β-Actin (0.03 µg/ml, Cat. # 4967, RRID: AB_330288; Cell Signaling Technology). Next ...
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bioRxiv - Neuroscience 2023Quote: ... and rabbit polyclonal anti-α-tubulin antibody (2 µg/ml; 2144, Cell Signaling Technology) overnight at 4 °C ...
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bioRxiv - Genetics 2021Quote: ... The blots were washed overnight before being incubated with a 1:5000 dilution of anti-actin-HRP for two hours at room temperature (Cell Signaling, 13E5).
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bioRxiv - Plant Biology 2020Quote: ... MAPK phosphorylation was detected via immunoblotting using an antiphospho-p44/42 MAPK antibody (dilution 1:5000 in TBST, Cell Signaling Technology, USA) as primary and HRP-conjugated anti-rabbit IgG (1:10000 in TBST ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were washed three times with TBS-T buffer and incubated at RT for 1 hour with horseradish peroxidase-conjugated secondary anti-mouse or anti-rabbit antibodies (1:5000; Cell Signaling Technology). The membranes were washed three times in TBS-T ...
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bioRxiv - Molecular Biology 2021Quote: ... Detection was performed with horseradish peroxidase-conjugated secondary antibodies: horse anti-mouse (1:5000; Cell Signaling, USA, Cat. No. Cat. No. 7076S), goat anti-rat (1:5000 ...
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bioRxiv - Immunology 2022Quote: ... Membranes were then washed and incubated in blocking buffer containing HRP-linked horse anti-mouse (1:5000; Cell Signaling Technology Cat#7076) for 45 minutes at room temperature ...
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bioRxiv - Physiology 2022Quote: ... The membranes were subsequently washed 4 times in TBS-T for 10 min before incubating in the appropriate secondary antibody anti-rabbit HRP (1:5000; 1677074P2, Cell Signaling Technology) or anti-mouse HRP (1:5000 ...
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bioRxiv - Biochemistry 2022Quote: ... gels were transferred to Nitrocelllose membranes and probed with anti-CFTR (ab596 from Cystic Fibrosis Foundation, 1:1750) or anti-Nav 1.5 (D9J7S, Cell Signaling, 1:5000) overnight in 5% milk in TBST ...
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bioRxiv - Plant Biology 2024Quote: ... After addition of a 1:5000 dilution of horseradish peroxidase-conjugated horse anti-mouse secondary antibodies (Catalog #7076; Cell Signaling Technology, USA), proteins were detected using an ECL kit (LI-COR Biosciences ...
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bioRxiv - Cell Biology 2024Quote: ... then probed overnight with our custom rabbit polyclonal anti-Abcg2a antibody (diluted 1:5000) and rabbit monoclonal anti-beta actin (Cell Signaling Technologies, Danvers ...
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bioRxiv - Cancer Biology 2024Quote: ... membranes were washed 3X with TBST for a total of 15 minutes and incubated with HRP conjugated secondary anti-mouse (7076S) or anti-rabbit (7074S) antibodies at a 1:5000 ratio (Cell Signaling Technology) for 1 hour at room temperature with gentle rocking ...
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bioRxiv - Plant Biology 2023Quote: ... GST-PpROP4 was detected with an anti-GST primary antibody (26H1) of mouse origin (Cell Signaling Technology, cat. 2624-100µl, 5000× dilution) and an anti-mouse-HRP (goat ...
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bioRxiv - Cancer Biology 2023Quote: ... 1:3000), anti-p21 Waf1/Cip1 (clone 12D1, #2947, 1:3000), anti-Vinculin (clone E1E9V, #13901, 1:5000) were from Cell Signaling Technology. Goat anti-rabbit antibody linked to horseradish peroxidase (HRP ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were then washed in TTBS/1% skim milk, incubated with secondary antibodies (1:5000 anti-mouse IgG, HRP-linked antibody (Cell Signaling #7076S)) ...
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bioRxiv - Cancer Biology 2023Quote: ... incubation for 1–3 hr at RT with 1:5000 secondary antibody (Cell Signaling Technology goat HRP-linked Anti rabbit IgG #7074), and 3 more 5 min washes with TBST ...
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bioRxiv - Genomics 2023Quote: ... Membranes were washed in TBST and incubated 1 hour at room temperature with secondary antibody diluted 1:5000 in 5% milk / TBST [anti-rabbit IgG HRP (Cell Signaling 7074S)] ...
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bioRxiv - Biophysics 2023Quote: ... The membranes were incubated overnight at 4 °C with primary antibodies against VDAC1 and β-Actin (1:5000, #2146; Cell Signaling Technology) after the washing step with secondary antibodies HRP-conjugate Goat anti-Mouse IgG (H+L ...
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bioRxiv - Cancer Biology 2024Quote: ... 1:1000, Sigma; anti-DCTPP1 antibody, 1:500, Abcam; anti-XPB antibody, 1:500, Abcam; anti-β-actin antibody, 1:5000 Cell Signaling) overnight at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... They were then incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies [HRP-conjugated anti-mouse IgG (1:5000; Cell Signaling Technology; #7076) and HRP-conjugated anti-rabbit IgG (1:5000 ...
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bioRxiv - Cancer Biology 2024Quote: ... The coverslips were then incubated with primary antibodies against BioID2 (1:5000, BioFront #BID2-CP-100) or HA tag (1:800, Cell Signaling #3724) diluted in 1% BSA/ PBS-Tween 0.1% for one hour in a humidified chamber at RT ...
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bioRxiv - Biochemistry 2022Quote: ... Immunoblotting was performed according to standard procedures using the following primary antibodies: DNMT3A (#D2H4B, Cell Signaling Technology, 1:5,000), DNMT3B (#D7070 ...
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bioRxiv - Cell Biology 2024Quote: ... and the standard immunocytochemistry technique described above was carried out using 1:1000 anti-NFk-B (Cell Signaling Technology) and 1:1000 Alexa Fluor 568 donkey anti-rabbit (Invitrogen) ...
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bioRxiv - Cell Biology 2021Quote: ... ChIP-Grade protein G magnetic beads (Cell Signaling, 9006S - 25 μL per IP) were pre-blocked in pre-blocking buffer (PBS/EDTA containing 0.5% BSA ...
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bioRxiv - Neuroscience 2023Quote: ... Lysates were co-imunoprecipitated with anti-HA beads (HA-Tag C29F4 Rabbit mAb Sepharose® Bead Conjugate Cell Signaling #3956 RRID:AB_10695091) overnight at 4 °C under gentle agitation ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins of interest were detected with HRP-conjugated horse anti-mouse IgG antibody (1:5000, 7076S, RRID:AB_330924, Cell Signaling Technology, Danvers, MA, USA) or HRP-conjugated goat anti-rabbit IgG antibody (1:5000 ...
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bioRxiv - Genetics 2022Quote: ... the blots were washed and then probed with the respective HRP-conjugated secondary antibodies at 1:5000 (Cell Signaling Technology, 7076 and 7074). Immuno-bands were subsequently detected by the enhanced chemiluminescence reaction (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2021Quote: ... Membranes were rinsed with TBST for 30 min before incubation with horseradish peroxidase-conjugated anti-rabbit secondary antibody (Cell Signaling; 7074S, 1:5000) for 1 h ...
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bioRxiv - Immunology 2022Quote: ... for 2h then incubated with primary antibodies (1:5000) overnight: anti-HA antibody (rabbit polyclonal antibody; Sinobiological #11684-T62) and anti-β-actin antibody (mouse monoclonal antibody; Cell Signaling #3700). The membrane was washed with TBS – Tween 20 0.1% ...
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bioRxiv - Developmental Biology 2023Quote: ... Membranes were then washed three times in TBST and incubated with appropriate horseradish peroxidase–conjugated secondary antibodies at 1:5000 dilution in TBST (Cell Signaling Technology, US) for 1 h at room temperature ...
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bioRxiv - Physiology 2023Quote: ... was heated at 90°C for 5 minutes and loaded into acrylamide gels before incubated with primary antibodies against catalase (CAT, #14097, Cell Signaling, 1:5000), 3-nitrotyrosine (3-NT ...
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bioRxiv - Neuroscience 2023Quote: ... The membrane was then washed in TBS-Tween three times for 10 min at RT and incubated with the secondary antibody anti-mouse-HRP (1:5000) (Cell Signaling, Danvers, MA) for one hour at RT ...
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bioRxiv - Bioengineering 2023Quote: ... secondary antibodies were incubated for 1 hour at room temperature (Secondary antibodies: Anti-Rabbit HRP-conjugated Cell Signaling Technology (CST-#7074S 1:5000); Anti-Mouse HRP-conjugated (R&D ...
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bioRxiv - Physiology 2024Quote: ... the membranes were incubated with gentle agitation for 1 h at room temperature using the following secondary antibodies conjugated to a chemiluminescence entity: anti-rabbit IgG and HRP-linked antibodies (1:5000, Cell Signaling Technology, 7074). WSE-6100 Lumino-GraphI (ATTO Corporation ...