Labshake search
Citations for Cytoskeleton :
101 - 150 of 155 citations for Rat Colony Assay Media since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... GTP-pulldown and western blot procedures were performed according to RHOA/RAC1/CDC42 Activation Assay Combo kit (Cytoskeleton) manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: Activated Rac1 was measured using the absorbance-based G-LISA Rac1 Activation Assay Biochem Kit (BK128, Cytoskeleton, USA). Briefly ...
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bioRxiv - Cell Biology 2019Quote: MT polymerization assays were performed using a fluorescence-based Tubulin Polymerization kit (Cytoskeleton Inc, Denver CO, Cat. # BK011P) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... cells lysates were prepared according to manufacturer’s instructions (RhoA Pull-Down Activation Assay Biochem Kit; Cytoskeleton Inc., BK036). Specifically ...
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bioRxiv - Cell Biology 2020Quote: Rac1 activation was measured by means of a G-LISA Activation Assay with luminescence read-out (Cytoskeleton, Inc.) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: The microtubules binding experiments were performed with the Microtubule Binding Protein Spin-Down Assay Biochem Kit (Cytoskeleton, Inc.) following manufacturer protocol.
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bioRxiv - Pharmacology and Toxicology 2021Quote: Active RhoA levels were measured using the RhoA Pull-down Activation Assay Biochem Kit (BK036-S, Cytoskeleton Inc.) using GST Rhotekin beads ...
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bioRxiv - Cancer Biology 2021Quote: RHOA activity was measured on LN-F lysates using the RHOA G-LISA Activation Assay kit (#BK124, Cytoskeleton). Data are the mean ± SEM of arbitrary units of RHOA activity.
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bioRxiv - Developmental Biology 2019Quote: Actin pelleting experiments were performed using the G-Actin/F-actin In Vivo Assay Biochem Kit (Cytoskeleton, Inc.) with modifications ...
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bioRxiv - Cell Biology 2024Quote: ... Rho-GTP was measured using the RhoA G-LISA Activation Assay Kit (Colorimetric Format; Cat# BK124) from Cytoskeleton, according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2021Quote: ... the Rac1- and cdc42 Small GTPase Activation Assay kits (G-LISA) were used (027BK127-S, 027BK128-S, Cytoskeleton, Inc) following the company’s guidelines.
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bioRxiv - Neuroscience 2021Quote: Effects of tau and nocodazole on MT polymerization were tested using a Tubulin Polymerization Assay (Cytoskeleton Inc., Denver, CO). Briefly ...
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bioRxiv - Cell Biology 2021Quote: The pyrene-actin polymerization and depolymerization assays were performed using the Actin Polymerization Biochem Kit (Cytoskeleton, Inc., Denver, CO) following the manufacturer protocol ...
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bioRxiv - Systems Biology 2022Quote: The Rho activity was measured and quantified by using the RhoA Activation Assay Biochem Kit (cat. no. BK036; Cytoskeleton) based on the Rhotekin pull-down assay as per the manufacturer’s instructions as described previously(39) ...
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bioRxiv - Biochemistry 2022Quote: ... colorimetric Rac1 and RhoA G-LISA Activation Assay Kits (BK128-S and BK124-S Cytoskeleton Inc., Denver CO, USA) were used according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: Actin protein levels were calculated using a G-actin/F-actin in vivo Assay Biochem Kit as per manufacturer’s instructions (Cytoskeleton). Briefly ...
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bioRxiv - Immunology 2021Quote: ... GTP-bound form of CDC42 was pulled down for analysis using the Cdc42 Pull-Down Activation Assay Kit (Cytoskeleton). FLAG-tagged protein pull-down assays were performed using DDDDK tagged protein purification kit (MBL) ...
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bioRxiv - Cancer Biology 2023Quote: GTP-bound RAS levels were assessed with a RAS G-LISA Assay Kit per manufacturer’s instructions (Cytoskeleton INC., #BK131). 9 ξ 105 cells were plated in a 3D 24-well plate ...
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bioRxiv - Cell Biology 2023Quote: ... GTP-bound ARF6 was detected in cell lysates using the ARF6 Activation Assay Biochem Kit™ (Cytoskeleton, BK-033) according to manufacturer’s instructions.
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bioRxiv - Biophysics 2020Quote: ... Rac1-GTP and Cdc42-GTP using the RhoA and Cdc42 pull-down activation assay biochem kit (PN:BK036-S and BK034-S, Cytoskeleton, Inc.) following the protocol provided by the manufacturer ...
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bioRxiv - Molecular Biology 2021Quote: ... the pathway was activated with PDGF-AA at 50 ng/ml for 20 minutes at 37°C and the G-LISA assay (Cytoskeleton) was performed following the manufacturer’s instructions ...
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Rab35 Governs Apicobasal Polarity Through Regulation of Actin Dynamics During Sprouting AngiogenesisbioRxiv - Developmental Biology 2022Quote: Globular and filamentous actin ratios were determined by western blot as described by commercially available G-actin/ F-actin In Vivo Assay Kit (Cytoskeleton). Globular and filamentous immunocytochemistry was performed as previously described [53] ...
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bioRxiv - Biochemistry 2021Quote: The co-sedimentation assay was adapted from the in vitro polymerization assay from (Venkei et al. 2006) as follows: 60 μM porcine brain tubulin (Cytoskeleton) was polymerised into microtubules in BRB80 buffer (1x ...
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bioRxiv - Cancer Biology 2020Quote: ... Lysate preparation and measurement of RHOA-GTP levels were carried out with the RHOA G-LISA activation assay kit (#BK124, Cytoskeleton) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: G- and F-actin were fractionated and enriched using a commercial in vivo assay biochem kit (Cytoskeleton Inc, Colorado, USA), according to the manufacturer’s instructions with minor modifications ...
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bioRxiv - Developmental Biology 2022Quote: ... Protein lysates were centrifugated for 15 min at > 15000 rpm at 4°C and supernatant was collected and quantified by Precision Red Advanced Protein Assay (Cytoskeleton). 5-10 μg of protein were boiled in NuPAGE LDS sample buffer (Invitrogen ...
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bioRxiv - Microbiology 2019Quote: ... Whole colonic tissue was homogenized in cell lysis buffer and protein concentration was determined using the Precision Red Protein Assay (Cytoskeleton). Samples were sent to Eve Technologies for analysis using their 32-multiplex Mouse Cytokine/Chemokine Array.
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bioRxiv - Molecular Biology 2020Quote: ... Intracellular Rac1 activity was determined using a Rac1 G-LISA Activation Assay Kit (Colorimetric format) (Cytoskeleton, catalog BK128, Denver, CO) using 25μg total protein per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Intracellular RhoA activity was determined using a RhoA G-LISA Activation Assay Kit (Colorimetric format) (Cytoskeleton, catalog BK124, Denver, CO) using 20μg total protein per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: Rac1 and RhoA GTPase actitation was measured by G-LISA Rac1 activation assay Kit and G-LISA RhoA activation Kit (Cytoskeleton), according to manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... cleaved proteins (quantified via a Bradford Assay) were incubated at room temperature for one hour with 0 or 5μM polymerized chicken F-actin (Cytoskeleton, Inc.). BSA was used a negative control ...
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bioRxiv - Immunology 2020Quote: The protocol of G and F actin measurement mostly followed the instructions of the “G-actin /F-actin In Vivo Assay Kit” from Cytoskeleton Inc ...
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bioRxiv - Cell Biology 2022Quote: ... and Rac or Rho activity was assessed in duplicate using the G-LISA small G-protein activation assay kit (BK128, BK124, Cytoskeleton) according to the manufacturer’s directions ...
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bioRxiv - Cell Biology 2022Quote: Guanine nucleotide exchange (GEF) activity of ERK3 (9-327 aa) was further evaluated using RhoGEF Exchange Assay (Cat# BK100, Cytoskeleton). Assay was performed according to the manufactures’s instructions and included Dbs GEF as a positive control.
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bioRxiv - Neuroscience 2023Quote: ... the cells were lysed in 250 µl cell lysis buffer supplemented with protease inhibitors and subjected to a pull-down assay for activated Rac1 (#BK035, Cytoskeleton). Briefly ...
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bioRxiv - Developmental Biology 2023Quote: ... Supernatant was collected upon 15min of centrifugation at >15000 rpm at 4°C and quantified by Precision Red Advanced Protein Assay (Cytoskeleton). Equal amounts of protein were boiled in NuPAGE LDS sample buffer (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: RhoAGTP and Rac1GTP pull down as-says were performed using the RhoA and Rac1 Activation Assay Biochem Kit (Cytoskeleton BK030) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: Activation of ARF6 (ARF6-GTP) was quantitated using the Arf6 Pull-Down Activation Assay Biochem Kit from Cytoskeleton (BK033-S, USA) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... GAP assays were performed as described previously (Faix et al., 1998) except that GTP hydrolysis was quantitated using CytoPhos reagent (Cytoskeleton Inc) to measure the amount of free phosphate ...
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bioRxiv - Neuroscience 2021Quote: Hippocampal and cortical tissue from WT and HTZ mice were lysed and homogenized in conditions that stabilize filamentous (F-) and monomeric (G-) actin using an F/G actin commercial assay (Cytoskeleton Inc.). Lysed extracts were ultracentrifuged at 100,000 g for 1 h at 37 °C in order to separate pellets (F-actin ...
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Importin α/β Promote Kif18B Microtubule Association to Spatially Control Microtubule DestabilizationbioRxiv - Cell Biology 2022Quote: Dynamic MT seed assays were performed by polymerizing GMPCPP stabilized Cy5-MT seeds from 4 µM HiLyte 647 tubulin (Cytoskeleton, #TL670M) in BD buffer + 0.5 mM GMPCPP at 37°C for 1 hour ...
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bioRxiv - Microbiology 2022Quote: ... 110 μl of LAS2 buffer was prepared according to the protocol of a G-actin/F-actin In Vivo Assay Biochem Kit (Cytoskeleton, Inc.) and mixed with the pellet ...
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bioRxiv - Cell Biology 2023Quote: The F-actin/G-actin ratio was measured using G-Actin/F-Actin In Vivo Assay Biochem Kit (#BK037, Cytoskeleton, Inc.), following the manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: F- and G-actin fractions from lysates were prepared using a G-Actin/F-Actin in Vivo Assay Biochem kit (Cytoskeleton, #BK037) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Western blots to determine the ratio of G-actin to F-actin were performed on retinal lysates that were processed using a G-actin/F-actin In Vivo Assay Kit (Cytoskeleton, Inc.) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: Active GTP-bound GTPase levels in brain lysates were measured using G-LISA activation assay kits for Rac1 (Cytoskeleton, Inc., BK128) and RhoA (Cytoskeleton ...
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bioRxiv - Neuroscience 2020Quote: ... and RAC1 were performed through the use of an ELISA-based Rho-GTPase activation assay kit (Cytoskeleton, Inc, catalog# BK124, BK127, BK128). Briefly ...
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bioRxiv - Microbiology 2019Quote: ... 5 μL of the filtered supernatant was pipetted into a clear flat-bottom UV plate and 250 μL of 1x Advanced Protein Assay Reagent (Cytoskeleton Inc., CO) was added and mixed ...
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bioRxiv - Cell Biology 2023Quote: Measurements of the microtubule-stimulated ATPase activities of KIF1A MD-NC-CC1-FHA and various mutants were performed by using the HTS Kinesin ATPase Endpoint Assay Biochem Kit (Cytoskeleton, Inc., BK053). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: ... Total protein was extracted from multiple cell cultures (G411, G729, G797) using the RAC1 Activation Assay Biochem Kit per manufacturer’s instructions (Cytoskeleton Inc, #BK035-S) five days post transduction with lentiviral non-targeting or GJB2 shRNA ...