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Citations for Cytoskeleton :
51 - 100 of 155 citations for Rat Colony Assay Media since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... Protein concentration was determined using APA assay (Cytoskeleton Inc. Denver, CO, USA) and 50 μg total protein were separated on SDS-PAGE followed by western blot ...
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bioRxiv - Cancer Biology 2021Quote: The microtubule polymerisation assay was performed using porcine neuronal tubulin (Cytoskeleton, Inc, BK006P) as an adaptation of the original method of Shelanski et al ...
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bioRxiv - Molecular Biology 2021Quote: Rho-GTPase pull-down assays were performed following the manufacturer’s protocol (Cytoskeleton, Inc.). Briefly ...
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bioRxiv - Biochemistry 2022Quote: ... The dGAE protein concentration was measured using Advanced Protein Assay Reagent (Cytoskeleton, Inc.) with bovine serum albumin as a standard ...
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bioRxiv - Microbiology 2022Quote: Rac activation was measured with a Rac123-G-LISA assay (absorbance based; Cytoskeleton) and performed according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: RAS activity was analyzed with a Ras Activation Assay Biochem Kit (Cytoskeleton, BK008). The QGY cell lines containing the control group ...
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bioRxiv - Cell Biology 2020Quote: Microtubule polymerization assays were performed using 1.5 mg/mL purified tubulin (Cytoskeleton, Inc) that was taken from a 10 mg/mL flash frozen stock ...
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bioRxiv - Cell Biology 2022Quote: Actin polymerization assay was performed using actin polymerization biochem kit (Cat# BK003, Cytoskeleton) according to the provided protocol ...
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bioRxiv - Neuroscience 2023Quote: ... and the Cdc42 pull-down activation assay kit (#BK034) were purchased from Cytoskeleton, Inc ...
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bioRxiv - Cell Biology 2023Quote: The high throughput screen (HTS) tubulin polymerization assay kit (Cat. BKOO4P, Cytoskeleton Inc.) was carried out as per the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... we used a MT binding protein spin-down assay kit (# BK029, Cytoskeleton, Inc.) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... RhoA activity was detected using the RhoA G-LISA Activation Assay Kit (Cytoskeleton) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2019Quote: ... The final assay solution contained pM concentrations of 10 - 15% biotinylated actin (Cytoskeleton Inc,) from rabbit skeletal muscle stabilized by rhodamine phalloidin ...
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bioRxiv - Cell Biology 2022Quote: ... Protein concentrations were determined using the Precision Red Advanced Protein Assay Reagent (Cytoskeleton ADV02). Extracted proteins (25-35 μg ...
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bioRxiv - Cell Biology 2019Quote: ... a G-LISA RhoA Activation Assay Biochem kit (# BK124; Cytoskeleton Inc., Denver, CO, USA) was used according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... and protein concentration determined using the Precision Red™ Advanced Protein Assay Reagent (Cytoskeleton). Respective samples were treated with 300nM Nocodazole for 15 min before lysis ...
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Autocrine Sfrp1 inhibits lung fibroblast invasion during transition to injury induced myofibroblastsbioRxiv - Cell Biology 2022Quote: Activation of RhoA was quantified using the RhoA G-LISA assay kit (Cytoskeleton Inc.) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: Rac1 activity assay was performed according to the manufacturer’s protocol (Cat# BK035, Cytoskeleton Inc). DBTRG or HF2303 cells were transfected with Rac1-WT ...
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bioRxiv - Pathology 2023Quote: ... MT assays were performed using the MT Binding Protein Spin-down kit from Cytoskeleton, Inc ...
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bioRxiv - Microbiology 2022Quote: ... we used the RhoA/Rac1/Cdc42 Activation Assay Combo Biochem Kit (Cytoskeleton, Inc., BK030). The pull-down was performed following manufacturer’s instructions.
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bioRxiv - Pharmacology and Toxicology 2023Quote: In vitro tubulin polymerization assays were performed according to the manufacturer’s instructions (Cytoskeleton, Inc.) optimized for polymerization inhibitors ...
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bioRxiv - Cell Biology 2020Quote: ... the drug-containing complete DMEM was replaced by the imaging media containing the same drug and 500 nM SiR-DNA (CY-SC007, Cytoskeleton) + 5 μM Verapamil ...
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bioRxiv - Neuroscience 2020Quote: ... and protein concentration estimated in the supernatant using Precision Red Advanced Protein Assay (Cytoskeleton, CA). Samples were prepared at 25 µg protein/well with 2X laemmli loading buffer and 2-mercaptoethanol and run on pre-cast 4-15% gels (Biorad) ...
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bioRxiv - Developmental Biology 2020Quote: ... total cellular active RhoA levels were measured using the Rho Activity Assay (Cytoskeleton Inc., BK036) following the manufacturer’s guidelines ...
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bioRxiv - Cancer Biology 2022Quote: We performed Rac1–GTP pull-down assay using the Rac1 activation kit (# BK035-S, Cytoskeleton) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: RhoA activities were determined using G-LISA™ RhoA Activation Assay Biochem Kit™ (Cytoskeleton) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... For microtubules specific fractionation we used a microtubules/tubulin in vivo assay kit (Cytoskeleton, #BK038) according to vendor specifications ...
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bioRxiv - Cell Biology 2022Quote: The Microtubule Binding Protein Spin-down assay was performed according to the manufacturers’ protocol (Cytoskeleton). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: RAC1 activation was examined using the Cdc42 Activation Assay Biochem Kit (BK034, Cytoskeleton, Denver, USA) following them anufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... The following steps were performed using the Cytoskeleton Ras-activation assay biochem kit (Cytoskeleton, #BK008) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were then used for G-LISA small GTPase activation assay (Cytoskeleton cat# BK135), where levels of activated RhoA ...
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bioRxiv - Immunology 2023Quote: ... Protein concentrations of crude extracts were determined using the Advanced Protein Assay (Cytoskeleton, Denver, USA).
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bioRxiv - Cell Biology 2023Quote: ... Polymerization assays were performed using a mixture of commercial rhodamine labeled actin monomers (Cytoskeleton, Inc.) and unlabeled actin monomers that were mixed at a 1:1 ratio ...
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bioRxiv - Neuroscience 2023Quote: ... we used the G-actin/F-actin in vivo assay kit (Cytoskeleton Inc., Denver, USA) according to manufacturer’s protocol ...
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bioRxiv - Bioengineering 2024Quote: ... and samples were collected using the “Microtubules/Tubulin In Vivo Assay Kit” (Cytoskeleton, Cat. #BK038) as per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... and RhoA was measured using a Rho GTPase Activation Assay Combo Biochem Kit (BK030, Cytoskeleton, USA), according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2022Quote: The pyrene actin polymerization and depolymerization assays were performed using the Actin Polymerization Biochem Kit (Cytoskeleton) per the manufacturer’s protocol with modifications ...
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bioRxiv - Cell Biology 2022Quote: ... Cell lysates were harvested as outlined in the G-lisa RhoA Activation Assay Kit (Cytoskeleton Inc) using the supplied lysis buffer ...
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bioRxiv - Cell Biology 2019Quote: ... and protein concentrations were measured using the Precision Red Advanced Protein Assay Reagent (# ADV02; Cytoskeleton Inc.) and finally equalized with ice-cold lysis buffer to 1.0 mg/ml ...
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bioRxiv - Neuroscience 2023Quote: Rac1 activity was determined using the Rac1 Pull-Down Activation Assay Biochem Kit (Cytoskeleton, Inc., BK035), following manufacture’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... or Cdc42 activation in vivo assay biochemical kits for the respective protein (Cytoskeleton Inc., Denver, CO). The tissues were lysed in lysis buffer (50mM Tris-HCl pH7.4 ...
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bioRxiv - Immunology 2023Quote: ... RhoA GTPase activity was measured using the RhoA Pull-Down Activation Assay Biochem Kit (#BK036; Cytoskeleton) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Different types of labelled and unlabeled purified tubulin used in the assays were either purchased from Cytoskeleton or purified as described previously (57 ...
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bioRxiv - Molecular Biology 2022Quote: ... The levels of RhoA and RhoB activity were measured using the Rho Activation Assay Biochem Kit (Cytoskeleton) with Rhotekin pulldown according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: RhoA activity was calculated using a RhoA G-LISA Activation Assay kit as per manufacturer’s instructions (Cytoskeleton). Briefly ...
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bioRxiv - Molecular Biology 2022Quote: ... Active RhoA was detected in a colorimetric RhoA activation assay (G-LISA) (BK124, Cytoskeleton, Denver, CO, USA).
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bioRxiv - Cell Biology 2023Quote: ... All samples were processed using the “G-actin / F-actin In Vivo Assay Kit” (Cytoskeleton Inc., #BK037) which involves lysing cells in a filament-supporting buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Protein concentration of the cleared lysates was determined by Precision Red Advanced Protein Assay Reagent (Cytoskeleton Inc.). Equal amounts of total cell lysates were loaded to 4–12% Bis-Tris or 3–8% Tris-Acetate gels (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... and pten– cells was assessed using a pull-down assay kit (Cat. #BK008-S; Cytoskeleton, Denver, CO) following the manufacturer’s instructions as previously described (Kirolos and Gomer ...
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bioRxiv - Neuroscience 2024Quote: ... G- and F-actin was detected by G-Actin/F-Actin In Vivo Assay Biochem Kit (Cytoskeleton) according to the manufacturer’s protocol ...