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Citations for Cytoskeleton :
1 - 50 of 155 citations for Rat Colony Assay Media since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... media was replaced with Fluorobright fluorescent imaging media containing 500 nM sirDNA (Cytoskeleton). The chamber was then transferred to a Nikon Ti microscope fitted with an OKOlab environmental control chamber ...
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bioRxiv - Cancer Biology 2024Quote: ... The media was replaced with fresh media containing MemGlow 488 (Cytoskeleton; 1:200) and cells were incubated for 20 min at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... the cell culture media was replaced with CO2-independent media containing 100μM SiR-tubulin (Cytoskeleton). For conditions involving UMK57 (a kind gift from Duane Compton ...
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bioRxiv - Cancer Biology 2022Quote: ... a Ras GTP-ase immunosorbent assay (G-LISA) Activation Assay Kit (#BK131, Cytoskeleton) was used ...
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bioRxiv - Biochemistry 2024Quote: Cdc42-GEF activation assay (Cytoskeleton) was performed per manufacturer’s instructions (26) ...
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bioRxiv - Cell Biology 2020Quote: For GTP-RhoA pull down assay the Rho Activation Assay biochem kit (Cytoskeleton inc, Cat# BK036) was used as described in the product manual ...
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bioRxiv - Cell Biology 2020Quote: Rho activation assay was performed using a RhoA Pull-down Activation Assay Biochem Kit from Cytoskeleton Inc (BK036-S) ...
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bioRxiv - Molecular Biology 2019Quote: Co-pelleting assay was performed according to the F-actin binding co-sedimentation assay from Cytoskeleton Inc ...
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bioRxiv - Cell Biology 2021Quote: The Rac1 activation assay was conducted using the Rac1 Pull-Down Activation Assay Biochem Kit (Cytoskeleton) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: CytoPhos Endpoint Phosphate Assay (Cytoskeleton, BK054) was used to measure GTP hydrolysis ...
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Volumetric Compression Shifts Rho GTPase Balance and Induces Mechanobiological Cell State TransitionbioRxiv - Biophysics 2023Quote: Colorimetric-based GLISA assays (Cytoskeleton, Inc.) were used to quantify RhoA and Rac1 activity ...
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bioRxiv - Cell Biology 2024Quote: ... media was replaced with DMEM containing 1:4000 SPY650-DNA (Cytoskeleton) at least one hour before imaging.
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bioRxiv - Cell Biology 2021Quote: Cdc42 and Rac1 Pull-Down assays were carried out using Cdc42/Rac1 Activation Assay Biochem Kits (Cytoskeleton, inc), following manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Rac and Cdc42 was measured in lysates from negatively sorted neutrophils from all genotypes by performing respective activation G-LISA assays (RhoA, Rac1,2,3 and Cdc42 G-LISA Activation Assays; Cytoskeleton) on fibronectin-coated plates as per manufacturer’s protocol.
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bioRxiv - Neuroscience 2023Quote: Actin bundling assays were carried out using the Actin Binding Protein Spin-Down Assay Biochem Kit (Cytoskeleton Inc.) according to protocol of the manufacturer.
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bioRxiv - Genetics 2024Quote: ... Protein quantification was determined via Bradford assay using PrecisionRed Advanced Protein Assay reagent (#ADV01, Cytoskeleton, Denver, CO, USA). Lysate concentration was normalized before dilution in 2X Laemmli Sample buffer with 10% v/v 2-mercaptoethanol ...
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bioRxiv - Molecular Biology 2020Quote: RhoA-GTP pulldown activation assays (Cytoskeleton, #BK036) were carried out according to protocol ...
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bioRxiv - Cell Biology 2020Quote: ... and G-LISA assay was performed as per the manufacturer’s instructions (Rac1 and Cdc42 G-LISA activation assay kits, Cytoskeleton).
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bioRxiv - Plant Biology 2021Quote: F-actin disruption assays were carried out using the Actin Binding Protein Spin-Down Assay Biochem kit (Cytoskeleton, USA) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... In vitro microtubule-binding assay was performed using the microtubule-binding protein spin down assay kit (Cytoskeleton, Denver, CO, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Precision Red Advanced Protein Assay (ADV02-A; Cytoskeleton), calcein AM (C1430 ...
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bioRxiv - Neuroscience 2021Quote: ... assays were carried out using kits from Cytoskeleton, Inc ...
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bioRxiv - Cell Biology 2020Quote: ... and released into fresh media containing 100 nM SiR-DNA stain (Cytoskeleton Inc.). Cells were imaged live five-hours post release for 18 hours using an ImageXpress XL imaging system (Molecular Devices ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated in MEDIA supplemented with 1 μM SiR-Tubulin (Cytoskeleton, Inc) for 30-60 minutes prior to washing out the SiR-Tubulin with fresh DMEM before imaging ...
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bioRxiv - Neuroscience 2019Quote: G/F-actin assay was performed according to the manufacturer’s instructions (G/F-actin In Vivo Assay Kit, Cytoskeleton, Denver, CO). G-and F-actin bands on western blots were scanned by densitometry and the ratios of free G-actin to F-actin were calculated.
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bioRxiv - Neuroscience 2022Quote: Rac1 activation was measured by using a Rac1 activation assay kit (Rac1 Pull-down Activation Assay Biochem Kit bead pulldown format, Cytoskeleton, catalog #BK035), following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... hippocampal slices previously stabilized for 1 h with oxygenated ACSF were lysed and homogenized in the presence of conditions that stabilize F-actin and G-actin using an F/G actin commercial assay (F/G actin in vivo assay BK037, Cytoskeleton Inc., USA). Then ...
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bioRxiv - Cancer Biology 2024Quote: ... The RhoActivity assay was performed using the RhoA pull-down activation assay biochem kit (Bead pull-down format) (Cytoskeleton, Cat No. BK036). The assay was carried out by incubating 800 µg of total cell lysate with 50 µg of Rhotekin as described in the manufacturers protocol following which western blots were performed ...
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bioRxiv - Biochemistry 2020Quote: ... was measured using Advanced Protein Assay Reagent (Cytoskeleton, Inc.) with bovine serum albumin as a standard ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein concentration was determined using APA assay (Cytoskeleton Inc.) and 50 μg total protein were separated on SDS-PAGE followed by western blot ...
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bioRxiv - Cancer Biology 2022Quote: RAS Activation Assay Biochem Kit™ (BK008; Cytoskeleton, Inc) was used to assess KRAS activity following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration was measured with Precision Red Assay (Cytoskeleton). Laemmli sample buffer (Biorad ...
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bioRxiv - Cancer Biology 2022Quote: ... the RAS-GTP pull-down assay kit (Cytoskeleton #BK008) was used ...
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bioRxiv - Genetics 2024Quote: ... Protein concentration was determined using APA assay (Cytoskeleton Inc.) and 50 - 100 μg total protein was separated on SDS-PAGE followed by western blot ...
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated in culture media containing SiR-Tubulin (Spirochrome/Cytoskeleton Inc., Denver, CO). Single cell suspensions were generated as above ...
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bioRxiv - Cancer Biology 2019Quote: The G-actin/F-actin assay was performed with a G-actin/F-actin in vivo assay kit (Cytoskeleton, Inc., Denver, CO, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... 3 G-LISA™ Activation Assay (purchased from Cytoskeleton Inc.), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... For ubiquitin pulldown assays using the SignalSeeker kit (Cytoskeleton Inc), cells were lysed in provided BlastR buffer with protease inhibitor and NEM ...
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bioRxiv - Cancer Biology 2022Quote: ... the colorimetric-based RAS G-LISA activation assay (Cytoskeleton #BK131) was used ...
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bioRxiv - Cell Biology 2020Quote: ... and then placed in 1.5 ml imaging media containing 1 μM SiR-DNA (CY-SC007, Cytoskeleton) + 10 μM Verapamil (for nucleus staining ...
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bioRxiv - Biophysics 2023Quote: ... cells were incubated in fresh hormone depletion media supplemented with 1µM Sir-Hoechst (Cytoskeleton, Cy-SC007). Cells were placed on the microscope in hormone depletion media supplemented with 1nM Sir-Hoechst ...
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bioRxiv - Cell Biology 2019Quote: ... colorimetric G-LISA activity assay kit (BK128-S, Cytoskeleton, Denver, Colorado) was used according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: Bulk Rac1 activity was measured using an assay kit (BK035, Cytoskeleton), as previously described (Hara et al. ...
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bioRxiv - Neuroscience 2022Quote: ... G-LISA RhoA activation assay biochem kit (Absorbance based) (Cytoskeleton, BK124) was used according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2022Quote: The RhoA Activation Assay Biochem Kit (bead pull-down format, Cytoskeleton) was used for performing the pull-down assay on 2D monolayers that were either uninduced or induced for the (DN ...
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bioRxiv - Cell Biology 2023Quote: RhoA GAP assays were performed using a kit provided by Cytoskeleton Inc (Denver Colorado Cat no ...
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bioRxiv - Developmental Biology 2023Quote: ... Protein quantification was performed using Precision Red Protein Assay Reagent (Cytoskeleton) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein concentration was determined using Precision Red Advanced Protein Assay (Cytoskeleton, Inc.). 20ug protein were loaded onto 8-12% SDS-PAGE gels and transferred onto nitrocellulose membranes ...
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bioRxiv - Biophysics 2022Quote: The G-actin/F-actin In Vivo Assay Kit (cat. #BK037, Cytoskeleton) was used to determine the F/G-actin ratio ...
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bioRxiv - Cell Biology 2020Quote: Colchicine-competition assays were performed with 2 μM purified tubulin (Cytoskeleton, Inc) taken from a 10 mg/mL stock prepared in tubulin buffer as above ...