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Citations for Cytoskeleton :
101 - 150 of 1141 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... 5 μg of GST-Rac1 (Cytoskeleton, Inc. RCG01-C) was charged with GDP or GTPγS by incubating for 20 minutes at 37°C in 100 μl GST pulldown buffer (20 mM Tris-HCl pH 7.4 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... or SiR-Actin (1:1000, Cytoskeleton, Inc. CY-SC001). We note that anti-Kaede immunoreactivity occurs in a subset of endodermal neurons (Supplementary Fig ...
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bioRxiv - Developmental Biology 2024Quote: ... Filamentous actin (F-actin) was labeled using Acti-stain 488 phalloidin or Acti-stain 555 phalloidin (1:200; Cytoskeleton). This thorough immunofluorescence staining protocol ensures accuracy and reliability ...
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bioRxiv - Microbiology 2024Quote: ... cells were stained using sirDNA (Cytoskeleton, 150 nM) for 2 hours prior to imaging to visualize chromosomal DNA ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were stained prior to imaging for 20 minutes at 37°C with 500 nM tetramethylrhodamine methyl ester (TMRM) and 500 nM SiR-Tubulin (Cytoskeleton: CY-SC002). All images were acquired in three-dimensional (3D ...
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bioRxiv - Plant Biology 2024Quote: ... (BSA02, Cytoskeleton Inc.,). To prepare the proteins for testing ...
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bioRxiv - Plant Biology 2024Quote: ... 0.4 mg Pre-formed Actin Filaments (AKF99, Cytoskeleton Inc. ...
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bioRxiv - Plant Biology 2024Quote: In vitro microtubule binding assay was carried out with Microtubule Binding Protein Spin-Down Assay Biochem kit (Cytoskeleton BK029) by following the manufacturer instructions ...
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bioRxiv - Neuroscience 2024Quote: ... permeabilized with 0.5% Triton-X and stained with Hoechst and phalloidin rhodamine (Cytoskeleton, Inc) following the manufacturers protocol.
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bioRxiv - Cell Biology 2024Quote: ... The sample was then treated for 30min at room temperature with 1mg/ml S1 fragments derived from rabbit psoas skeletal muscle (Cytoskeleton Incorporated, Denver, CO; CS-MYS04), reconstituted in PEM buffer containing 2.0µM phalloidin and centrifuged at 100,000 x g for 15min at 4°C prior to use ...
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bioRxiv - Cell Biology 2024Quote: RhoA activation assay was performed using RhoA G-LISA Activation Assay Kit – colorimetric format (Cytoskeleton Inc) following manufacturer’s instruction ...
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bioRxiv - Neuroscience 2024Quote: ... and Acti-StainTM 670 Phalloidin to mark neurites (200 nM; Cytoskeleton Inc.; Denver, CO; Cat #: PHDN1-A). The following day ...
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bioRxiv - Biophysics 2024Quote: ... Rho activator I (CN01) (Cytoskeleton) was used at a concentration of 1 unit/mL ...
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bioRxiv - Biophysics 2024Quote: ... in culture media for mitochondria staining or a 1000x dilution manufacturer recommended stock concentration of SPY555-DNA (Cytoskeleton inc.) in culture media for histone staining for 30 min ...
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bioRxiv - Biophysics 2024Quote: ... Nanofiber networks were sterilized with 70% ethanol and functionalized with 4 μg/mL of rhodamine-conjugated fibronectin (Cytoskeleton Inc.) in PBS for 1 hr to enable cell-fiber attachment.
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bioRxiv - Neuroscience 2024Quote: ... The immunoprecipitation assay was performed using Ubiquitin Detection Kit (Cytoskeleton, BK161) as mentioned in the manufacturer’s protocol with some modifications ...
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bioRxiv - Neuroscience 2024Quote: ... Deubiquitination inhibitors provided with Ubiquitin Detection Kit (Cytoskeleton, BK161) were also added to the lysis buffer at 1X concentration ...
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bioRxiv - Biophysics 2024Quote: ... Arp2/3 (from porcine brain, #RP01P) and cdc42 (his-tagged, human constitutively active mutant Q61L, #C6101-A) were purchased from Cytoskeleton Inc.
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bioRxiv - Biochemistry 2024Quote: ... obtained either from Cytoskeleton or purified in-house ...
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bioRxiv - Biochemistry 2024Quote: ... Taxolstabilized microtubules assembled from unlabelled porcine tubulin (Cytoskeleton) were introduced into the chamber ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were additionally fixed by adding 50 µl of the same fixation buffer that was now supplemented with a low concentration to Tween-20 (Cytoskeleton Buffer with 3% PFA and 0.07% Tween-20), which we found to improve the the preservation of cellular structures throughout subsequent step ...
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bioRxiv - Bioengineering 2024Quote: ... The selective adsorption of fibronectin to patterned surfaces was visualized using fluorescently labeled (green) fibronectin (Cytoskeleton, Inc, Denver, CO). Cells began to align in the pattern direction 2 hrs after seeding (Fig ...
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bioRxiv - Neuroscience 2024Quote: ... and a 642 nm laser was used for JF635 or JF646 HaloTag-ligands and SirActin (SiR-Actin Kit, Cytoskeleton Inc, CY-SC001). Fluorescence emission was detected by pco.edge CLHS sCMOS cameras with appropriate bandpass filters LBF 405/488/561/642 + SBS LP 560 nm ...
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bioRxiv - Developmental Biology 2024Quote: ... The fixation was quenched with the addition of 50 µl of quench buffer (Cytoskeleton Buffer with 0.3M glycine, pH 6.1). Immediately after ...
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bioRxiv - Biophysics 2024Quote: Porcine brain tubulin (Cytoskeleton Cat # T240) was reconstituted to 10 mg/mL in BRB80 buffer (80 mM Pipes ...
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bioRxiv - Biophysics 2024Quote: ... 1 mM GTP (cat. BST06, Cytoskeleton Inc.), and 10% glycerol ...
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bioRxiv - Bioengineering 2024Quote: ... SMCy5.5 (Bio-Techne #7295) and SiR-DNA (SiR-Hoechst, Cytoskeleton Inc.CY-SC007) were used throughout this work ...
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bioRxiv - Biophysics 2024Quote: ... was diluted to 1 μM in the tubulin buffer or the presence of 2 mg/ml tubulin protein (cat. T240, Cytoskeleton Inc.) or 0.1% SDS ...
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bioRxiv - Biochemistry 2024Quote: Rabbit skeletal muscle G-actin (AKL99) was purchased from Cytoskeleton. Inc and polymerized into F- actin according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... The following fluorescent probes were used in this study: SPY555-DNA (Cytoskeleton, Inc, #CY-SC201), SPY555-tubulin (Cytoskeleton ...
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bioRxiv - Cancer Biology 2024Quote: ... SPY555-tubulin (Cytoskeleton, Inc, #CY-SC203), SiR-DNA (2 nM ...
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bioRxiv - Biophysics 2024Quote: ... Rabbit muscle actin was purchased from Cytoskeleton.
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bioRxiv - Cancer Biology 2024Quote: ... SiR-DNA (2 nM, Cytoskeleton, Inc, #CY-SC007), SiR-Tubulin (20 nM ...
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bioRxiv - Cell Biology 2024Quote: ... Nuclear counterstain DAPI or SiR-700 DNA (Cy-SC015, Cytoskeleton, Inc.) was imaged with a laser at 405 nm or 647 nm ...
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bioRxiv - Immunology 2024Quote: ... RhoA pull-down activation assay Biochem Kit (BK036-S) was purchased from Cytoskeleton, Inc ...
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bioRxiv - Pathology 2024Quote: Pyrene-labeled actin was purchased from Cytoskeleton Inc ...
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bioRxiv - Cell Biology 2024Quote: ... taxol-stabilised microtubules were prepared by mixing 2.5 ml of 100 μM porcine tubulin (Cytoskeleton) resuspended in BRB80 with 1 mM GTP (Cytoskeleton) ...
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bioRxiv - Neuroscience 2024Quote: ... and 1:100 protease inhibitor cocktail) and then resuspended in 800 μl Rac1 lysis buffer with 10 μg GST-PAK-PBD (Cytoskeleton, Inc. PAK01-A). GST-PAK-PBD was conjugated to beads by rotating for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration of the supernatant was quantified by Precision Red assay (Cytoskeleton, Inc. ADV02). For GDP and GTP-charged controls ...
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bioRxiv - Plant Biology 2024Quote: ... microtubules were obtained from tubulin (T240, Cytoskeleton). All proteins were centrifuged at 100,000 × g for 30 min at 4°C before use ...
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bioRxiv - Physiology 2024Quote: ... Actin-stain 555 phalloidin (Cat# PHDH1) was purchased from Cytoskeleton, Inc ...
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bioRxiv - Neuroscience 2024Quote: ... pre-formed microtubules from Cytoskeleton (Denver, CO, USA), and MG132 from EMD Millipore (Burlington ...
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bioRxiv - Neuroscience 2024Quote: ... Taxol-stabilized microtubules (Cytoskeleton, Cat# MT002) at a final concentration of 2.5 μg/ml ...
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bioRxiv - Neuroscience 2024Quote: ... for quantification with Precision Red (Cytoskeleton, Inc. #ADV02-A) according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2024Quote: ... 16% X-rhodamine tubulin (Cytoskeleton, TL620M-A) or Hi-Lyte 647 Tubulin (Cytoskeleton ...
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bioRxiv - Cell Biology 2024Quote: ... and 22% biotin-tubulin (Cytoskeleton, T333PA) with 0.7 mM GMPCPP (Jena Biosciences ...
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bioRxiv - Cell Biology 2024Quote: ... Fluorescently labelled MT seeds were generated by mixing 62% porcine brain tubulin (Cytoskeleton, T240B), 16% X-rhodamine tubulin (Cytoskeleton ...
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bioRxiv - Bioengineering 2024Quote: ... at least three disks per sample were stained with phalloidin (Cytoskeleton Inc., Denver, CO, USA) and DAPI (MilliporeSigma ...
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bioRxiv - Bioengineering 2024Quote: ... MemGlowTM 488 (Cytoskeleton, Cat. # MG01) served as an additional pan EV label in PBE EV quantification experiments ...
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bioRxiv - Cell Biology 2024Quote: ... ATP (Cytoskeleton, BSA04001) was added in a concentration of 0.5 mM.