Labshake search
Citations for Cytoskeleton :
1 - 50 of 1141 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2024Quote: ... we stained cells with SPY555-actin (1:1,000; Cytoskeleton CY-SC202) at 37 °C for 1 hr ...
-
bioRxiv - Cancer Biology 2024Quote: ... phenol-red free DMEM and incubated with MemGlow dyes (MemGlow 560, MG02-2 and MemGlow 590, MG03-10; Cytoskeleton) in serum-free ...
-
bioRxiv - Cancer Biology 2024Quote: ... cells were labeled by adding the dyes MemGlow 488 (Cytoskeleton; 1:200), SPY555-Actin (Cytoskeleton ...
-
bioRxiv - Cancer Biology 2024Quote: ... SPY555-Actin (Cytoskeleton; 1:1,000), and NucSpot Live 650 (Biotium ...
-
bioRxiv - Cancer Biology 2024Quote: ... The cells were labeled with the dyes SPY555-Actin (Cytoskeleton; 1:1,000) and NucSpot Live 650 (Biotium ...
-
bioRxiv - Cancer Biology 2024Quote: ... The media was replaced with fresh media containing MemGlow 488 (Cytoskeleton; 1:200) and cells were incubated for 20 min at 4 °C ...
-
bioRxiv - Cell Biology 2024Quote: ... Rho/Rac/Cdc42 Activator I (10 μM; Cytoskeleton, Inc #CN04-A). All treatments were applied for a duration of 1 hour prior to fixation with 4% PFA ...
-
bioRxiv - Cell Biology 2024Quote: ... Rho Inhibitor I (C3 Transferase from Clostridium botulinum covalently linked to a cell penetrating moiety; 10μM; Cytoskeleton, Inc #CT04-A); Rac GTPase ...
-
bioRxiv - Genetics 2024Quote: ... Protein quantification was determined via Bradford assay using PrecisionRed Advanced Protein Assay reagent (#ADV01, Cytoskeleton, Denver, CO, USA). Lysate concentration was normalized before dilution in 2X Laemmli Sample buffer with 10% v/v 2-mercaptoethanol ...
-
bioRxiv - Genetics 2024Quote: ... Protein concentration was determined using APA assay (Cytoskeleton Inc.) and 50 - 100 μg total protein was separated on SDS-PAGE followed by western blot ...
-
bioRxiv - Cell Biology 2024Quote: ... fibronectin-HiLyte488 (FNR02 Cytoskeleton, ∼ 230 kg/mol); heparin (H3149-100KU ...
-
bioRxiv - Biochemistry 2024Quote: ... and 1% HiLyte FluorTM 488-labeled tubulin (Cytoskeleton, Inc. #TL488M) were used as an internal control in all chambers for all assays ...
-
bioRxiv - Biochemistry 2024Quote: ... Taxol-stabilized human unmodified microtubules assembled with 1.5% biotinylated tubulin (Cytoskeleton, Inc. #T333P) and 1% HiLyte FluorTM 488-labeled tubulin (Cytoskeleton ...
-
bioRxiv - Biochemistry 2024Quote: Cdc42-GEF activation assay (Cytoskeleton) was performed per manufacturer’s instructions (26) ...
-
bioRxiv - Biochemistry 2024Quote: ... and His-DBS (DH/PH domain) were purchased from Cytoskeleton, Inc ...
-
bioRxiv - Biochemistry 2024Quote: ... The cells were lysed and protein lysates were used for G-LISA analysis per manufacturer’s (Cytoskeleton) instructions ...
-
The T-cell niche tunes immune function through modulation of the cytoskeleton and TCR-antigen forcesbioRxiv - Bioengineering 2024Quote: ... SiR-Actin (# CY-SC001) was purchased from Cytoskeleton, Inc ...
-
The T-cell niche tunes immune function through modulation of the cytoskeleton and TCR-antigen forcesbioRxiv - Bioengineering 2024Quote: ... and SiR-Actin (Cytoskeleton, Inc # CY-SC001). Samples were run on Amnis ImagestreamX MK II at 60x in EDF mode and data was analyzed in IDEAS software.
-
The T-cell niche tunes immune function through modulation of the cytoskeleton and TCR-antigen forcesbioRxiv - Bioengineering 2024Quote: ... then 0.5 μM SirActin (Cytoskeleton, Inc, # CY-SC001) in 1% BSA in PBS was added to each well for 30 minutes ...
-
bioRxiv - Developmental Biology 2024Quote: ... sir-actin (1/2000, Cytoskeleton, n°CY-SC001) and DAPI for 1h at RT ...
-
bioRxiv - Genomics 2024Quote: ... and 500 nM SiR-tubulin Kit (Cytoskeleton, Denver, Colorado, U.S.) according to the manufacturer’s instructions.
-
bioRxiv - Biochemistry 2024Quote: ... filaments of 0.3 nM TMR-phalloidin-labelled biotinylated actin (Cytoskeleton, Denver, CO, USA), 0.018 mg/mL catalase ...
-
bioRxiv - Biophysics 2024Quote: ... or biotin tubulin (Cytoskeleton) were added (6% w/w ...
-
bioRxiv - Cancer Biology 2024Quote: ... and 2‰ MemGlow 560 (Fluorogenic Membrane Probe, Cytoskeleton), and imaged on the imaging setup described below.
-
bioRxiv - Cell Biology 2024Quote: ... and 1 µm verapamil (Cytoskeleton, CY-SCV01)
-
bioRxiv - Cell Biology 2024Quote: ... cells were treated with 1 µm sirDNA (Cytoskeleton, CY-SC007) and 1 µm verapamil (Cytoskeleton ...
-
bioRxiv - Cell Biology 2024Quote: ... anti-SUMO2/3 (Asm23, Cytoskeleton, 1:200 dilution), and anti-PML (sc966 ...
-
bioRxiv - Cell Biology 2024Quote: ... or HiLyte647-labeled porcine tubulin (all from Cytoskeleton) was incubated with 1 mM GMPCPP (Jena Biosciences ...
-
bioRxiv - Cell Biology 2024Quote: ... Biotin-labeled tubulin was purchased from Cytoskeleton Inc ...
-
bioRxiv - Developmental Biology 2024Quote: ... or Acti-stain 488 fluorescent Phalloidin (Cytoskeleton, Inc.) in PBST for 20 min ...
-
bioRxiv - Neuroscience 2024Quote: ... RhoA activity was detected using the RhoA G-LISA Activation Assay Kit (Cytoskeleton) according to the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2024Quote: E15 DIV hippocampus neurons described above were transfected with EGFP-OPTN or EGFP-OPTNΔC 24 hrs before staining with SPY555-tubulin (Cytoskeleton. Inc) for SIM imaging ...
-
bioRxiv - Neuroscience 2024Quote: ... G- and F-actin was detected by G-Actin/F-Actin In Vivo Assay Biochem Kit (Cytoskeleton) according to the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2024Quote: ... Microtubules were polymerized in vitro from purified tubulin dimers (Cytoskeleton, Inc,; Zeng et al., 2010). To evaluate whether CAPS coprecipitated with polymerized microtubules ...
-
bioRxiv - Developmental Biology 2024Quote: ... Cysts were incubated in a culture medium containing SPY650-actin (Cytoskeleton, Inc., 1:2000 dilution) for 3 h before indentation ...
-
bioRxiv - Cell Biology 2024Quote: ... and pten– cells was assessed using a pull-down assay kit (Cat. #BK008-S; Cytoskeleton, Denver, CO) following the manufacturer’s instructions as previously described (Kirolos and Gomer ...
-
bioRxiv - Cell Biology 2024Quote: ... Rho-Activator II (Cytoskeleton CN03, 2µg/ml), or ROCK inhibitor y-s7632 (Peprotech 1293823 ...
-
bioRxiv - Cell Biology 2024Quote: ... To quantify lysosome distribution in circular RPE cells plated on fibronectin ring micropatterns containing rhodamine fibronectin (FNR01; Cytoskeleton Inc.) that were generated as described31 ...
-
bioRxiv - Developmental Biology 2024Quote: ... Oocytes were stained with 150 nM Sir-Tubulin (CY-SC002, Cytoskeleton) and Hoechst 33342 to visualize spindle and chromosomes ...
-
bioRxiv - Cell Biology 2024Quote: ... The protein was then transferred to a clean tube and quantified using PrecisionRed (Cytoskeleton; ADV02-A) at OD600.
-
bioRxiv - Cell Biology 2024Quote: ... Soluble proteins were collected after a 10 minute centrifugation step at 15000 rpm and protein concentration was measured using PrecisionRed (Cytoskeleton; ADV02). 1.5 mg of protein was mixed with 25 μl of pre-equilibrated GFP-Trap_A beads (ChromoTek ...
-
bioRxiv - Cell Biology 2024Quote: ... the cilia of cultured multiciliated cells were live-labeled using SiR-tubulin (Cytoskeleton, #CY-SC002) or Spy555-tubulin (Cytoskeleton ...
-
bioRxiv - Cell Biology 2024Quote: ... or Spy555-tubulin (Cytoskeleton, #SC203). The apical surface was washed with 2% NS culture medium (2% NuSerum ...
-
bioRxiv - Cell Biology 2024Quote: ... Cell nuclei were visualized via SPY650-DNA (Cytoskeleton, cat. #: CY-SC501) probe that was incubated for 2 hours at 37° C at a 1:1,000 dilution in complete media ...
-
bioRxiv - Cell Biology 2024Quote: ... For staining with SiR-tubulin dye (Cytoskeleton, Inc., cat. #CY-SC002), culture medium was supplemented with 1 µM of SiR-tubulin ...
-
bioRxiv - Microbiology 2024Quote: ... Pre-formed actin filaments (Cytoskeleton, Inc.) were handled according to the manufacturing instructions ...
-
bioRxiv - Cell Biology 2024Quote: ... Biotin-actin was purchased from Cytoskeleton, Inc (Denver ...
-
bioRxiv - Cell Biology 2024Quote: ... Rho Activator II (Cytoskeleton, Inc., Cat. #CN03). We started live-imaging about 2-3 hours after seeding cells ...
-
bioRxiv - Cell Biology 2024Quote: ... RhoA Activator II (1 µg/mL, #CN03 Cytoskeleton, Inc.), or a vehicle (DMSO ...
-
bioRxiv - Biophysics 2024Quote: ... GST-tagged WCA domain of human WASP protein (GST-WCA) was purchased from Cytoskeleton (Cat. #VCG03-A) and used without further purification ...