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Citations for Cytoskeleton :
1 - 50 of 1155 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... media was replaced with DMEM containing 1:4000 SPY650-DNA (Cytoskeleton) at least one hour before imaging.
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bioRxiv - Cell Biology 2024Quote: ... and pten– cells was assessed using a pull-down assay kit (Cat. #BK008-S; Cytoskeleton, Denver, CO) following the manufacturer’s instructions as previously described (Kirolos and Gomer ...
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bioRxiv - Cell Biology 2024Quote: ... To quantify lysosome distribution in circular RPE cells plated on fibronectin ring micropatterns containing rhodamine fibronectin (FNR01; Cytoskeleton Inc.) that were generated as described31 ...
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bioRxiv - Developmental Biology 2024Quote: ... Oocytes were stained with 150 nM Sir-Tubulin (CY-SC002, Cytoskeleton) and Hoechst 33342 to visualize spindle and chromosomes ...
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bioRxiv - Microbiology 2024Quote: ... Pre-formed actin filaments (Cytoskeleton, Inc.) were handled according to the manufacturing instructions ...
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bioRxiv - Bioengineering 2024Quote: ... Rhodamine Phalloidin (Cytoskeleton, Inc.), anti-YAP antibody IgG2a (mouse ...
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bioRxiv - Bioengineering 2024Quote: ... or incubated with SiR-Tubulin (Cytoskeleton, CY-SC002) or PKMO48 for ∼20 mins without wash before imaging.
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bioRxiv - Bioengineering 2024Quote: ... SPY650-DNA (Cytoskeleton, CY-SC501) was diluted to 1:1000 in PBS for ∼1 h and washed 3 times in PBS.
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bioRxiv - Bioengineering 2024Quote: ... and samples were collected using the “Microtubules/Tubulin In Vivo Assay Kit” (Cytoskeleton, Cat. #BK038) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: Pulldown of GTP-RhoA was performed according to the manufacturer’s instruction (Cytoskeleton; catalog #BK030). In brief ...
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bioRxiv - Neuroscience 2024Quote: ... and RhoA (Cytoskeleton, Inc., #BK124). Brains were homogenized in G-LISA lysis buffer supplemented with protease and phosphatase inhibitors (Roche) ...
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bioRxiv - Biophysics 2024Quote: ... GST-tagged WCA domain of human WASP protein (GST-WCA) was purchased from Cytoskeleton (Cat. #VCG03-A) and used without further purification ...
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bioRxiv - Neuroscience 2024Quote: Active GTP-bound GTPase levels in brain lysates were measured using G-LISA activation assay kits for Rac1 (Cytoskeleton, Inc., BK128) and RhoA (Cytoskeleton ...
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bioRxiv - Developmental Biology 2024Quote: ... Cysts were incubated in a culture medium containing SPY650-actin (Cytoskeleton, Inc., 1:2000 dilution) for 3 h before indentation ...
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bioRxiv - Cell Biology 2024Quote: ... Microtubules were polymerized in vitro from purified tubulin dimers (Cytoskeleton, Inc,; Zeng et al., 2010). To evaluate whether CAPS coprecipitated with polymerized microtubules ...
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bioRxiv - Cell Biology 2024Quote: ... Rho activator II (CN03) and SiR-Actin (CY-SC001) from Cytoskeleton; nocodazole (M1404 ...
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bioRxiv - Biochemistry 2024Quote: ... we used a MT binding protein spin-down assay kit (# BK029, Cytoskeleton, Inc.) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... The RhoActivity assay was performed using the RhoA pull-down activation assay biochem kit (Bead pull-down format) (Cytoskeleton, Cat No. BK036). The assay was carried out by incubating 800 µg of total cell lysate with 50 µg of Rhotekin as described in the manufacturers protocol following which western blots were performed ...
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bioRxiv - Genetics 2024Quote: ... Protein concentration was determined using APA assay (Cytoskeleton Inc. Denver, CO, USA) and 50 μg total protein were separated on SDS-PAGE followed by western blot ...
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bioRxiv - Cell Biology 2024Quote: ... anti-SUMO2/3 (Asm23, Cytoskeleton, 1:200 dilution), and anti-PML (sc966 ...
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bioRxiv - Cell Biology 2024Quote: ... Rho/Rac/Cdc42 Activator I (10 μM; Cytoskeleton, Inc #CN04-A). All treatments were applied for a duration of 1 hour prior to fixation with 4% PFA ...
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bioRxiv - Cell Biology 2024Quote: ... Rho Inhibitor I (C3 Transferase from Clostridium botulinum covalently linked to a cell penetrating moiety; 10μM; Cytoskeleton, Inc #CT04-A); Rac GTPase ...
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bioRxiv - Cell Biology 2024Quote: ... or HiLyte647-labeled porcine tubulin (all from Cytoskeleton) was incubated with 1 mM GMPCPP (Jena Biosciences ...
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bioRxiv - Cell Biology 2024Quote: ... Biotin-labeled tubulin was purchased from Cytoskeleton Inc ...
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bioRxiv - Developmental Biology 2024Quote: ... or Acti-stain 488 fluorescent Phalloidin (Cytoskeleton, Inc.) in PBST for 20 min ...
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bioRxiv - Neuroscience 2024Quote: E15 DIV hippocampus neurons described above were transfected with EGFP-OPTN or EGFP-OPTNΔC 24 hrs before staining with SPY555-tubulin (Cytoskeleton. Inc) for SIM imaging ...
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bioRxiv - Neuroscience 2024Quote: ... G- and F-actin was detected by G-Actin/F-Actin In Vivo Assay Biochem Kit (Cytoskeleton) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... RhoA activity was detected using the RhoA G-LISA Activation Assay Kit (Cytoskeleton) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... phenol-red free DMEM and incubated with MemGlow dyes (MemGlow 560, MG02-2 and MemGlow 590, MG03-10; Cytoskeleton) in serum-free ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were labeled by adding the dyes MemGlow 488 (Cytoskeleton; 1:200), SPY555-Actin (Cytoskeleton ...
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bioRxiv - Cancer Biology 2024Quote: ... SPY555-Actin (Cytoskeleton; 1:1,000), and NucSpot Live 650 (Biotium ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells were labeled with the dyes SPY555-Actin (Cytoskeleton; 1:1,000) and NucSpot Live 650 (Biotium ...
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bioRxiv - Cancer Biology 2024Quote: ... The media was replaced with fresh media containing MemGlow 488 (Cytoskeleton; 1:200) and cells were incubated for 20 min at 4 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... we stained cells with SPY555-actin (1:1,000; Cytoskeleton CY-SC202) at 37 °C for 1 hr ...
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bioRxiv - Microbiology 2024Quote: Microtubules were made by mixing 4 μg of HiLyte Fluor 488 tubulin (Cytoskeleton), 4 μg of biotinylated tubulin (Cytoskeleton ...
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bioRxiv - Microbiology 2024Quote: ... 4 μg of biotinylated tubulin (Cytoskeleton) and 100 μg of unlabelled pig tubulin in BRB80 buffer (80 mM PIPES pH 6.8 ...
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bioRxiv - Cell Biology 2024Quote: ... The protein was then transferred to a clean tube and quantified using PrecisionRed (Cytoskeleton; ADV02-A) at OD600.
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bioRxiv - Cell Biology 2024Quote: ... Soluble proteins were collected after a 10 minute centrifugation step at 15000 rpm and protein concentration was measured using PrecisionRed (Cytoskeleton; ADV02). 1.5 mg of protein was mixed with 25 μl of pre-equilibrated GFP-Trap_A beads (ChromoTek ...
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bioRxiv - Cell Biology 2024Quote: ... the cilia of cultured multiciliated cells were live-labeled using SiR-tubulin (Cytoskeleton, #CY-SC002) or Spy555-tubulin (Cytoskeleton ...
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bioRxiv - Cell Biology 2024Quote: ... or Spy555-tubulin (Cytoskeleton, #SC203). The apical surface was washed with 2% NS culture medium (2% NuSerum ...
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bioRxiv - Cell Biology 2024Quote: ... Cell nuclei were visualized via SPY650-DNA (Cytoskeleton, cat. #: CY-SC501) probe that was incubated for 2 hours at 37° C at a 1:1,000 dilution in complete media ...
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bioRxiv - Cell Biology 2024Quote: ... For staining with SiR-tubulin dye (Cytoskeleton, Inc., cat. #CY-SC002), culture medium was supplemented with 1 µM of SiR-tubulin ...
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bioRxiv - Cell Biology 2024Quote: ... Rho-Activator II (Cytoskeleton CN03, 2µg/ml), or ROCK inhibitor y-s7632 (Peprotech 1293823 ...
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bioRxiv - Cell Biology 2024Quote: ... The cell-adhesive patterns were confirmed by coating with green fluorescent-HiLyte 488-Laminin (Cytoskeleton, #LMN02-A) (Extended Data Fig ...
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bioRxiv - Biochemistry 2024Quote: ... and 1% HiLyte FluorTM 488-labeled tubulin (Cytoskeleton, Inc. #TL488M) were used as an internal control in all chambers for all assays ...
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bioRxiv - Biochemistry 2024Quote: ... Taxol-stabilized human unmodified microtubules assembled with 1.5% biotinylated tubulin (Cytoskeleton, Inc. #T333P) and 1% HiLyte FluorTM 488-labeled tubulin (Cytoskeleton ...
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bioRxiv - Biochemistry 2024Quote: ... filaments of 0.3 nM TMR-phalloidin-labelled biotinylated actin (Cytoskeleton, Denver, CO, USA), 0.018 mg/mL catalase ...
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bioRxiv - Biochemistry 2024Quote: ... Normalized Lysates were incubated with 20μL of anti-FLAG affinity gel or anti-Acetyl-Lysine (mAb mix) Affinity Beads (Cytoskeleton, Inc., AAC04-beads) at 4°C for 2 h ...
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bioRxiv - Cell Biology 2024Quote: ... fibronectin-HiLyte488 (FNR02 Cytoskeleton, ∼ 230 kg/mol); heparin (H3149-100KU ...
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bioRxiv - Cell Biology 2024Quote: The live-cell staining dyes used are as follow: 100 nM SiR-Tubulin (Cytoskeleton, CY-SC002), 1 μM ER-tracker Red (Beyotime ...