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Citations for Cytoskeleton :
401 - 450 of 1141 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... and actin polymerization buffer are provided by Cytoskeleton (Cytoskeleton, catalogue no. BK003).
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bioRxiv - Cell Biology 2023Quote: ... 5% biotin-labeled tubulin (Cytoskeleton, T333P), and 90% unlabeled tubulin (Cytoskeleton ...
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bioRxiv - Biochemistry 2023Quote: ... or 10 nM SiR-actin (Cytoskeleton Inc.) titrated with F-actin ...
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Membrane-dependent actin polymerization mediated by the Legionella pneumophila effector protein MavHbioRxiv - Molecular Biology 2023Quote: ... Actin polymerization biochem kit (BK003) was purchased from Cytoskeleton Inc ...
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bioRxiv - Neuroscience 2023Quote: ... actin (Cytoskeleton, #AKL99-A) was resuspended in general actin buffer (5 mM Tris-HCl pH 8.0 and 0.2 mM CaCl2) ...
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bioRxiv - Microbiology 2023Quote: ... cytoskeleton marker (Cytoskeleton 18, ab133263) for detecting the soluble nuclear (SN ...
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bioRxiv - Biophysics 2023Quote: ... all experimental protocols were identical except 10% biotin-actin (Cytoskeleton) was added to 2 μM G-actin and stabilized with tetramethylrhodamine isothiocyanate-labeled phalloidin in KMg25 buffer (60 mM MOPS pH 7.0 ...
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Phosphorylation controls spatial and temporal activities of motor-PRC1 complexes to complete mitosisbioRxiv - Biochemistry 2023Quote: ... microtubule seeds containing 7% rhodamine-tubulin (Cytoskeleton Inc., TL590M-B) were polymerized in BRB80 (80 mM PIPES pH 6.9 ...
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bioRxiv - Biochemistry 2023Quote: ... Taxol-stabilized microtubules assembled from unlabeled porcine tubulin (Cytoskeleton) were introduced into the chamber ...
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bioRxiv - Biochemistry 2023Quote: ... was added to either supplemented actin buffer alone or in the presence of 0.4 mg mL-1 G-actin (cat. AKL99, Cytoskeleton Inc.). The final ligand concentration was 2.5 μM ...
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bioRxiv - Biochemistry 2023Quote: ... 5 mM guanidine carbonate and 1.2 mM ATP (from Actin Polymerization Buffer, cat. BSA02, and ATP, cat. BSA04, Cytoskeleton Inc.). Samples were incubated at 37 °C for 2 h.
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bioRxiv - Biochemistry 2023Quote: ... 0.2 mM CaCl2 (from General Actin Buffer, cat. BSA01, Cytoskeleton Inc.), 50 mM KCl ...
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bioRxiv - Biophysics 2023Quote: ... or α4A/β2A tubulins ∼4% biotin-tubulin (Cytoskeleton) and ∼4% rhodamine-tubulin (Cytoskeleton ...
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bioRxiv - Biophysics 2023Quote: ... and ∼4% rhodamine-tubulin (Cytoskeleton) were incubated with 1 mM GMPCPP or 1 mM GTP with 10 mM taxol at 37°C for 3 h ...
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bioRxiv - Immunology 2023Quote: ... phalloidin 555 (Acti-stain 555 Cytoskeleton Inc, #PHDH1-A) at 1:400 dilution was used ...
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bioRxiv - Cell Biology 2023Quote: ... Rho inhibitor: C3 exoenzyme (Cytoskeleton, CT04); ROCK inhibitor ...
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bioRxiv - Bioengineering 2023Quote: ... Lipids accumulated in HLOs and cytoskeleton were stained with 2 μM BODIPY® 493/503 and 1 μM SiR-Actin (Cytoskeleton Inc., CO, USA), respectively ...
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bioRxiv - Bioengineering 2023Quote: For Airy-SIM imaging: We use SiR Tubulin Kit (Cytoskeleton, CY-SC002) to label tubulin in live COS-7 cells under the conconcentration of 1μM ...
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bioRxiv - Bioengineering 2023Quote: ... The COS-7 cells were labelled with PKmito RED (Nanjing Genvivo Biotech Co., Ltd. PKMR-1, CY-SC052)/PKmito DEEP RED (Cytoskeleton, CY-SC055) 15 minutes in DMEM ...
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bioRxiv - Cell Biology 2023Quote: ... labeled at a 1:40 ratio with HiLyte Fluor 488 tubulin (Cytoskeleton, Denver, CO), were flowed into the chamber and immobilized by interaction with rigor kinesin-1E236A.
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bioRxiv - Cell Biology 2023Quote: EVs isolated by SEC were stained using MemGlow™ 488 Fluorogenic Membrane Probe (Cytoskeleton) used at final concentration of 0.02uM ...
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bioRxiv - Cell Biology 2023Quote: ... we used a colorimetric Rac 1,2,3-GLISA (Cytoskeleton Inc).
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bioRxiv - Cell Biology 2023Quote: ... Cells were treated with SiR-DNA (Cytoskeleton Inc.), plated and imaged in 4-well cover glass dishes (Cellvis) ...
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bioRxiv - Cell Biology 2023Quote: ... a reaction vessel was prepared on ice containing 3 mg/ml of >97% purified porcine tubulin (Cytoskeleton, Inc. (#T240)) and the substances being tested ...
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bioRxiv - Cell Biology 2023Quote: The high throughput screen (HTS) tubulin polymerization assay kit (Cat. BKOO4P, Cytoskeleton Inc.) was carried out as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The Rho activator CN03 was purchased from Cytoskeleton Inc ...
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bioRxiv - Cell Biology 2023Quote: The Rhotekin-RBD Beads (Binds Active Rho Proteins) from Cytoskeleton Inc ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Cysts were then incubated with 10 μM SiR-Actin59 (Cytoskeleton, Inc.) and Hoechst 33342 (Invitrogen ...
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bioRxiv - Developmental Biology 2023Quote: ... Protein quantification was performed using Precision Red Protein Assay Reagent (Cytoskeleton) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... Alexa Fluor 594-phalloidin staining (Cytoskeleton) for F-actin was carried out by incubation at 1:100 dilution in PBS containing 5% normal goat serum for 30 min after fixation and permeabilization ...
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bioRxiv - Developmental Biology 2023Quote: ... Supernatant was collected upon 15min of centrifugation at >15000 rpm at 4°C and quantified by Precision Red Advanced Protein Assay (Cytoskeleton). Equal amounts of protein were boiled in NuPAGE LDS sample buffer (Invitrogen ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 μg tubulin purified from porcine brain (Cytoskeleton, T240) was mixed with 0.5 μg/μl BSA in assay buffer (50 mM Tris ...
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bioRxiv - Developmental Biology 2023Quote: HiLyte647-labeled microtubules were polymerized from purified tubulin including 10% Hily647-labeled tubulin (Cytoskeleton) in BRB80 buffer (80 mM Pipes/KOH pH 6.8 ...
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bioRxiv - Cell Biology 2023Quote: ... which was 5% fluorescently labelled with HiLyte 488 or HiLyte 647 conjugated porcine brain tubulin (Cytoskeleton), was assembled at 40 μM at 1 mM GTP for 30 mins at 37°C ...
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CDK5 activity in retinal pigment epithelium contributes to gap junction dynamics during phagocytosisbioRxiv - Cell Biology 2023Quote: ... the samples were preincubated for either 3 h with Rho/Rac/Cdc42 Activator I (1 µg/ml, Catalog number CN04, Cytoskeleton Inc), 30 min with MEK inhibitor PD 98059 (50 µM ...
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bioRxiv - Cell Biology 2023Quote: ... Protein concentration of the cleared lysates was determined by Precision Red Advanced Protein Assay Reagent (Cytoskeleton Inc.). Equal amounts of total cell lysates were loaded to 4–12% Bis-Tris or 3–8% Tris-Acetate gels (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... Seed mixture was prepared as 26 μM of porcine brain tubulin containing 10% biotinylated tubulin and 10% HiLyte 488 tubulin (both from Cytoskeleton) in 1 mM GMPCPP in KPEM ...
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bioRxiv - Biochemistry 2023Quote: ... microtubules were polymerized from 5 mg mL-1 porcine tubulin (Cytoskeleton Inc., Denver, CO) at 37 °C for 20 min in BRB80 supplemented with 1 mM GTP and then diluted 1:10 in BRB80 + 20 µM taxol ...
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bioRxiv - Cell Biology 2023Quote: ... F-actin was polymerized from chicken gizzard (smooth muscle) muscle actin (Cytoskeleton, APHL99).
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bioRxiv - Cell Biology 2023Quote: In vitro actin polymerization was assessed using the actin polymerization kit (Cytoskeleton, BK003). Briefly ...
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bioRxiv - Cell Biology 2023Quote: Actin binding and polymerization were assessed using the actin binding protein kit (Cytoskeleton, BK013). Actin pellet gel bands were quantified by densitometry using FIJI.
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bioRxiv - Biochemistry 2023Quote: ... rhodamine- and Hi-Lyte647-labelled porcine tubulin were purchased from Cytoskeleton (T240 ...
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bioRxiv - Developmental Biology 2023Quote: ... ovaries were respectively incubated with SiR-actin (Cytoskeleton TM) at 1:150 and with Wheat Germ Agglutinin (WGA ...
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bioRxiv - Developmental Biology 2023Quote: ... rabbit Anti-Khc (AKIN02-A, Cytoskeleton) 1:250 ...
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bioRxiv - Cancer Biology 2023Quote: Human non-muscle actin (Cytoskeleton, # APHL99) was polymerized or de-polymerized according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... and Acti-Stain 555 Phalloidin (1:150; Cytoskeleton Inc., PHDH1-A) for 1 hour in staining buffer ...
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bioRxiv - Cell Biology 2023Quote: ... calpeptin was from Cytoskeleton. (+)-Biotin-(PEO)3-iodoacetamide (biotinylated iodoacetamide ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were then used for G-LISA small GTPase activation assay (Cytoskeleton cat# BK135), where levels of activated RhoA ...
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bioRxiv - Bioengineering 2023Quote: Confocal microscopy (810 and 880 LSM, Zeiss, Germany) was used to image Nanog-GFP or MemGlow 590 (20 nM, Cytoskeleton, US) labeled MDA-MB231 cells using either 63× or 40× ...
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bioRxiv - Evolutionary Biology 2023Quote: ... testes were stained with 2 μM SiR-Actin59 (Cytoskeleton, Inc.) for 2-3 hours at room temperature and incubated with Hoechst 33342 (Invitrogen ...