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Citations for Beckman :
101 - 150 of 199 citations for Siglec 15 Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... The ribosomes were further purified by centrifugation at 59,727 ×g (15 hours, 4 °C, SW 32.1 Ti rotor, Optima™ L-100 XP, Beckman Coulter) in a linear (15 – 30 % ...
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bioRxiv - Neuroscience 2023Quote: ... the crude lysate was subjected to iodixanol gradient (15%, 25%, 40% and 60%) ultracentrifugation using a Type 70Ti rotor (Beckman) at 490000 × g ...
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Faa1 membrane binding drives positive feedback in autophagosome biogenesis via fatty acid activationbioRxiv - Biochemistry 2023Quote: ... The reaction was centrifuged at 68 000 rpm for 15 min at 22 °C (Beckman-Coulter Optima MAX, TLA100 rotor). The supernatant and pellet were loaded separately on an SDS/polyacrylamide gel in equal amounts ...
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bioRxiv - Systems Biology 2020Quote: ... tetramethylrhodamine (TRITC)-conjugated anti-mouse IgM (#731781, Beckman Coulter), and Alexa-594-conjugated anti-rabbit IgG (A11012 ...
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bioRxiv - Immunology 2023Quote: ... for mouse samples or a CytoFLEX LX (Beckman Coulter) for human samples ...
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bioRxiv - Neuroscience 2020Quote: ... Tubes were capped then centrifuged for 15 minutes at 30,000xg in a JLA 16.250 fixed angle rotor (Beckman-Coulter, Brea, CA) at 4°C using a Beckman Avanti JE centrifuge ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Populations were transferred twice a week by diluting 15% of the culture into 800 µL of fresh medium using a liquid-handling robot (Biomek NXP Span-8; Beckman Coulter). At each transfer ...
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bioRxiv - Biochemistry 2021Quote: ... 2 mM DTT) and centrifuged for 2 hours and 15 minutes at 39000 rpm at 4°C (Beckman Coulter TLS55 rotor). Fractions corresponding to the large mitochondrial subunit were collected and subjected to buffer exchange (25 mM HEPES-KOH pH = 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... starting with 50% and topping off with 15% for a total of 11 mL was added to thin wall polypropylene ultracentrifuge tubes (Beckman Coulter). The tubes were immediately frozen and stored at -80 °C ...
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bioRxiv - Cell Biology 2020Quote: ... The first PCR reaction was performed for 15 cycles with 60 °C annealing and 30 s elongation and then purified with AMPure XP PCR beads (Beckman Coulter). The second PCR was performed for 15 cycles for wild type-specific and for 21 cycles for tag-specific amplicons respectively using 60°C annealing and 30 s elongation ...
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bioRxiv - Biochemistry 2021Quote: ... The reaction mixtures were incubated at room temperature for 15 min followed by centrifugation at 36000 x g using Optima TL benchtop ultracentrifuge (Beckman Coulter) for 15 min at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... sucrose gradient and the RCII complexes were separated by ultracentrifugation (40,000×g,15 h) using an Optima XPN-90 Ultracentrifuge (Beckman Coulter, USA). The separated RCII/PSI fraction was collected and then washed repeatedly using the glycerol- and DM-free elution buffer and concentrated to 0.2 mg Chl/ml which corresponded to approximately 0.6 mg protein/ml.
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bioRxiv - Cell Biology 2022Quote: ... Thromboplastins and Fibrins the plasma was centrifuge at 4700Xg for 15 mins followed by high-speed centrifugation (Beckman Avanti J-26XP) at 12,000 x g for 25mins and filtered using a 0.22μm filter (Ultident Scientific ...
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bioRxiv - Biophysics 2019Quote: ... to final volume of 12 ml and then spun at 15°C for 16hr at 155,000g using a SW 41Ti rotor (Beckman Coulter, CA). The VLPs were detected at the lower part of the tube and recovered as described above ...
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bioRxiv - Systems Biology 2019Quote: ... The above-described double-stranded cDNAs were purified by using 15 μL of AMPure XP SPRI beads (Cat# A63881, Beckman Coulter) and a handmade 96-well magnetic stand for low volumes ...
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bioRxiv - Molecular Biology 2020Quote: ... The target library derived from the oocyte RNA polymerase II transcripts was PCR-amplified (15 cycles for P14 WT, 16 cycles for P14 Tbpl2−/− mutant) and purified using AMPure beads (Beckman Coulter) to remove short PCR artifacts (< 200bp ...
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bioRxiv - Biochemistry 2019Quote: 30S subunits were prepared from purified 70S ribosomes by sucrose gradient centrifugation in a zonal rotor (Ti-15, Beckman, CA, USA) under dissociating conditions using buffer TAKM3.5 (50 mM Tris (pH 7.5) ...
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bioRxiv - Biophysics 2019Quote: ... Samples were loaded onto the gradient and spun at 15°C for 4h at 140,000g using a SW28 rotor (Beckman Coulter, CA). The VLPs appeared as an opaque band at the interface of the 1.25 and 1.4 g/ml CsCl layers and were collected by piercing the tube with an 18G needle and syringe ...
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bioRxiv - Immunology 2021Quote: ... The PBMC were resuspended in 1 mL cold PBS+BSA per 15 mL whole blood processed and counted with a ViCell (Beckman Coulter) using VersaLyse reagent (Beckman Coulter #A09777 ...
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bioRxiv - Cell Biology 2022Quote: Whole bovine milk samples (200 mL) were centrifuged at 3,000 x g for 15 min at 4°C (Allegra X-15R, Beckman Coulter, France) to separate the fat from the skimmed milk ...
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bioRxiv - Biophysics 2022Quote: ... the 30S and 50S subunits were separated on a 15-35% (w/v) sucrose gradient in dissociation buffer on a SW-32 rotor (Beckman-Coulter) spun at 28,000 rpm (97,000 xg ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was recovered and centrifuged at 60,000 rpm for 15 min at 4°C in an Optima TL micro-ultracentrifuges (TLA 100.3 rotor Beckman Coulter, Fullerton, CA) to obtain the Golgi-enriched p100 pellet fraction ...
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bioRxiv - Microbiology 2023Quote: ... viruses were collected and concentrated by ultracentrifugation (37,000 rpm, 2 h 30, 15 °C; Beckman Type 45 Ti fixed-angle rotor). The virus titer was determined using a plaque assay.
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bioRxiv - Genomics 2022Quote: ... was added to each reaction and incubated for 15 minutes at 37 °C followed by 15 minutes at 80 °C and the samples were purified using 1x AMPure XP beads (Beckman Coulter). For the Panhandle protocol ...
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bioRxiv - Cell Biology 2023Quote: ... A volume of lysate containing 125 ug RNA was loaded on 11 ml 15-50% sucrose gradients in 14 x 89 mm centrifuge tubes (Beckman Coulter). (Gradients were prepared in 20 mM HEPES-KOH pH 7.4 ...
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bioRxiv - Genomics 2023Quote: ... Five million cells were harvested at 315xg for 7 minutes in a 15 mL centrifuge tube (Beckman Coulter Allegra X-14R) and stored at -80°C ...
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bioRxiv - Genomics 2023Quote: ... cellular/protein debris were then collected by centrifugation at 1,000xg for 15 minutes at 4°C (Beckman Coulter Allegra X-14R), and the supernatant (DNA ...
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bioRxiv - Biochemistry 2023Quote: ... Mitochondrial crude was pelleted by centrifugation at 9500 ×g (4 °C, 15 min) using the Optima XE-90 Ultracentrifuge with a Ti-45 rotor (Beckman Coulter). The final pellet was resuspended in 2 mL of Resuspension buffer (250 mM sucrose ...
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bioRxiv - Biochemistry 2023Quote: ... then centrifuged at 27,000 gmax for 15 min at 4°C (Optima XPN-100 Ultracentrifuge, Beckman Coulter, with SW60 Ti rotor). Resuspension of the pellet (sub-mitochondrial particles ...
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bioRxiv - Cancer Biology 2024Quote: ... using a combined Annealing/Extension step at 63°C for 10 seconds and 15 cycles followed by a 1.1X post-amplification AMPure XP (Beckman Coulter, A63880) bead cleanup ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1U of FastAP (TFS, EF0654) for 15 min at 37 °C and purified using 1.2× SPRIselect beads (Beckman Coulter, B23318). The inDrops-v1.1 libraries were digested with 40U of ExoI and 1U of FastAP for 15 min at 37 °C ...
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bioRxiv - Genomics 2024Quote: ... c) increasing PCR cycles (15 in total) and eliminating primer dimers prior to sequencing (Agencourt AMPure XP magnetic beads, Beckman Coulter). Primary PDAC were sequenced on the NovaSeq6000 ...
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bioRxiv - Microbiology 2020Quote: ... fluorescein isothiocyanate (FITC)-conjugated mouse monoclonal antibody KC57 (Beckman Coulter) was used at a dilution of 1:100.
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bioRxiv - Cell Biology 2023Quote: ... secondary anti-mouse-HRP (Beckman Coulter, Ref. 715-035-151), secondary anti-rabbit-HRP (Beckman Coulter ...
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bioRxiv - Biophysics 2021Quote: ... which were centrifuged at 3000 rpm for 15 minutes at 4°C using a fixed angle centrifuge (Beckman Coulter, Brea, CA, USA). After removal of excess supernatant ...
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bioRxiv - Cell Biology 2020Quote: ... The resulting supernatant containing AAV particles was added to the top of an iodixanol gradient (15%, 25%, 40%, 60% top to bottom) in an Optiseal tube (Beckman Coulter #361625). The gradient was then centrifuged using a Beckman Ti-70 rotor in a Beckman XL-90 ultracentrifuge at 67,000rpm for 70min ...
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bioRxiv - Cell Biology 2020Quote: ... tSETD2-Flag plasmid was transfected into HEK293 Freestyle cells with FectoPRO transfection reagent (116-010) and cells were harvested 36-48 hours later at 5,000 rpm for 15 mins (Beckman JLA 8.1, 363563). The pellet was suspended in lysis buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Neuroscience 2020Quote: ... Final 15 mL of solution was mixed by inverting the tube 10x and carefully pipetted into 2 ultracentrifuge tubes (Beckman Coulter #344059) onto the isolation buffer cushion (5 mL ...
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bioRxiv - Plant Biology 2021Quote: ... The resulting slurry was centrifuged for 15 min at 9000 rpm at 4 °C using a JA14 rotor (Beckman Coulter, Brea, CA). The supernatant was filtered with a Miracloth and kept on ice ...
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bioRxiv - Biochemistry 2020Quote: tSETD2-FLAG was transfected into HEK 293 Freestyle cells with FectoPRO transfection reagent (Polyplus, 10118-444) and cells were harvested 48 hours later at 5K rpm for 15 mins (Beckman JLA 8.1).The pellet was suspended in lysis buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... Harvesting of P2 viral stocks was carried out by centrifuging cell suspensions in 50 mL Falcon tubes at 2281 RCF (Beckman GS-15), collecting the supernatant in a fresh sterile tube ...
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bioRxiv - Cancer Biology 2021Quote: ... transferred to 96-well plates and incubated 15 min at room temperature with Agencourt AMPure XP magnetic beads (Beckman Coulter Cat# A63881) at a 1:1 ratio ...
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bioRxiv - Biochemistry 2021Quote: ... Harvesting of P2 viral stocks was carried out by centrifuging cell suspensions in 50 mL Falcon tubes at 2281 RCF (Beckman GS-15), collecting the supernatant in a fresh sterile tube ...
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bioRxiv - Cell Biology 2022Quote: ... The plate was incubated for 15 min in darkness and measured in the flow cytometer FC 500 (Beckman-Coulter, Brea, CA, USA). This experiment was repeated 3 times on different days.
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bioRxiv - Neuroscience 2022Quote: ... Cell lysate was then loaded on an iodixanol gradient (60, 40, 25 and 15% iodixanol, ELITechGroup #1114542) in Beckman Quick-Seal Polyallomer tubes (Beckman-Coulter #342414) which were sealed and centrifuged in a Beckman-Coulter Optima XE-90 Ultracentrifuge at 16 °C and 69,000 rpm for 1 hour and 10 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... Harvesting of P2 viral stocks was carried out by centrifuging cell suspensions in 50 mL Falcon tubes at 2281 RCF (Beckman GS-15). To the supernatant containing virus ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was collected and purified through 15-45% (w/v) sucrose density gradient by ultracentrifugation at 151,000 × g (using a 17 mL Beckman SW32.1 Ti rotor) for 3 hours at 10°C (18) ...
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bioRxiv - Microbiology 2022Quote: ... Supernatant was purified through 15-45% (w/v) sucrose density gradient by ultracentrifugation at 151,000 × g (using a Beckman SW 40 rotor) for 3 hours at 10°C (18).
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bioRxiv - Microbiology 2022Quote: ... Supernatant was purified through 15-45% (w/v) sucrose density gradient by ultracentrifugation at 151,000 × g (using a 17 mL Beckman SW32.1 Ti rotor) for 3 hours at 10°C (16) ...
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bioRxiv - Immunology 2023Quote: ... After adding 450 μL of RBC lysis solution (QuantoBio Technology, Beijing, China) and 15 min incubation the samples were analysed using a DxFLEX flow cytometer (Beckman Coulter, USA). All data were analysed using FlowJo X (FlowJo ...