Labshake search
Citations for Beckman :
51 - 100 of 199 citations for Siglec 15 Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2022Quote: Sucrose gradients (15-60%) were prepared in SW55Ti rotor-compatible Ultra-Clear ultracentrifuge tubes (Beckman Coulter, 344057). Briefly ...
-
bioRxiv - Biochemistry 2023Quote: ... The lysate was clarified by centrifuging at 14,000 rpm for 15 minutes (Beckman Coulter JA-20 rotor). Imidazole was added to the supernatant to final concentration of 10mM loaded onto a 5 mL HisTrap™ FF crude column (GE Healthcare ...
-
bioRxiv - Biochemistry 2023Quote: ... Cells were harvested by centrifugation at ∼6250g for 15 min at 4°C (Beckman Coulter centrifuge, JLA8.1000).
-
bioRxiv - Biochemistry 2023Quote: ... Samples were cleared by centrifuging at 5000 rpm for 15 min (Allegra® X-15R, Beckman-Coulter). Cleared lysates were pooled and 150μl of rabbit IgG-Agarose was added and incubated for 1h ...
-
bioRxiv - Immunology 2021Quote: ... The above-described double-stranded cDNA was purified using 15 μl of AMPure XP SPRI beads (Beckman Coulter) diluted 2-fold with Pooling buffer (20% PEG8000 ...
-
bioRxiv - Microbiology 2020Quote: ... Ribolysing matrix and cell debris were removed by centrifugation at 12,000 rpm (JA-15 1.5 rotor, Beckman Coulter) 4°C for 10 minutes ...
-
bioRxiv - Biochemistry 2020Quote: ... Then the ribosomes were pelleted through a sucrose cushion (1.1 M sucrose in HEPES:Polymix buffer with 15 mM Mg2+) at 50,000 rpm for two hours (TLS-55, Beckman), the pellet was dissolved in HEPES:Polymix buffer (5 mM Mg(OAc)2) ...
-
bioRxiv - Molecular Biology 2019Quote: ... The extracts were subjected to ultracentrifugation at 70 krpm for 15 min on a TLA100.3 rotor (Beckman-Coulter) to remove ribosomes ...
-
bioRxiv - Microbiology 2020Quote: ... Ribolysing matrix and cell debris were removed by centrifugation at 12,000 rpm (JA-15 1.5 rotor, Beckman Coulter) for 10 minutes.
-
bioRxiv - Cancer Biology 2022Quote: ... The captured libraries were amplified by PCR for 15 cycles and cleaned up using AMPure beads (Beckman Coulter).
-
bioRxiv - Biophysics 2022Quote: ... 72 hours of dialysis with tubings containing 15 kDa cutoff pores and subsequent ultracentrifugation (Optima LE-80K, Beckman Coulter Ultracentrifuge ...
-
bioRxiv - Molecular Biology 2023Quote: ... Cleared supernatants were loaded on a linear 15%–50% sucrose gradient and ultracentrifuged in a SW41Ti rotor (Beckman) for 1 hr and 30 min at 180,000 g at 4°C in a Beckman Optima LE-80K Ultracentrifuge ...
-
bioRxiv - Microbiology 2022Quote: ... Sacculi were repeatedly washed with MilliQ water by ultracentrifugation at ∼540,000 x g for 15 min at 20 °C in a TLA-100.3 rotor (OptimaTM Max ultracentrifuge, Beckman) until completely removing SDS traces ...
-
bioRxiv - Biophysics 2023Quote: ... 1 mM DTT) and spun at 32,600 × g for 15 h at 4° in SW32 rotor (Beckman Coulter). Ribosome fractions were pooled as shown in Figure 2D ...
-
bioRxiv - Cell Biology 2023Quote: ... The fraction containing TpPyShell was further concentrated by centrifugation (4000 g, 15 min, 4°C, SX4400 rotor; Beckman) with Amicon Ultra (M.W.4000 ...
-
bioRxiv - Bioengineering 2023Quote: ... The gradients were then ultracentrifuged at 120,000 x g for 15 h using SW41 Ti rotor from Beckman Coulter ...
-
bioRxiv - Biochemistry 2022Quote: ... The sample was than applied on a glycerol step gradient (30-12% glycerol) and centrifuged for 15 h at 25,000 rpm (SW 32 rotor, Beckman). The separation and distribution of proteins was confirmed by SDS-PAGE gel (4–20% Mini-PROTEAN® TGX™ Precast Protein Gels ...
-
bioRxiv - Biochemistry 2022Quote: ... Gradients were centrifuged at 42,800 x g for 15 h at 4 °C in an SW28 rotor (Beckman Coulter). Fractions of 1 mL were collected using an AKTA system (Cytiva) ...
-
bioRxiv - Microbiology 2021Quote: ... bacteria were harvested by centrifugation at 4000 rpm for 15 min (Microfuge 20R; Beckman Coulter, La Brea, CA, USA), the pellet was washed twice in phosphate-buffered saline (PBS ...
-
bioRxiv - Microbiology 2020Quote: ... The sample was vortexed for 10 s and incubated at room temperature for 5 minutes before centrifugation at 12,000 rpm (JA-15 1.5 rotor, Beckman Coulter) 4°C for 5 minutes ...
-
bioRxiv - Microbiology 2022Quote: ... 100 ml cell cultures were grown over night and harvested by centrifugation at 15,000 x g for 15 min at 4 °C (Beckman JLA-16.250 rotor ...
-
bioRxiv - Microbiology 2022Quote: ... 100 ml cell cultures were grown over night and harvested by centrifugation at 15,000 x g for 15 min at 4 °C (Beckman JLA-16.250 rotor, Beckman Avanti J-25, Beckman Coulter). Pellets were washed with 20 mM potassium phosphate (pH 7.5 ...
-
bioRxiv - Plant Biology 2022Quote: ... was gently layered on top of 4.4 mL sucrose gradient solution (15 to 55%) in a 5.1 mL Quick-Seal polypropylene tube (Beckman Coulter) and centrifuged at 45,000 rpm for 65 min at 4°C (Sorval NVT 90 ...
-
bioRxiv - Cell Biology 2023Quote: ... and 1 mM PMSF) and centrifuged for 15 hours at 4°C with 74,400 xg (SW 32.1 Ti; Beckman Coulter). 24 equal volume fractions were collected from the top and subjected to TCA precipitation ...
-
bioRxiv - Cell Biology 2023Quote: ... 20µM ATP and 1 mM DTT and subsequently sedimented (100,000 x g, 15 min, 4°C, TLA-100 rotor) in an Ultima MAX-XP ultracentrifuge (Beckman). For competition assays ...
-
bioRxiv - Biophysics 2023Quote: ... Cells were cultured for another 48 hours before harvest by centrifugation at 4,000 rpm for 15 min (JLA-8.1 rotor, Beckman Coulter). Cells were washed twice with phosphate buffered saline (PBS ...
-
bioRxiv - Microbiology 2023Quote: ... the sequencing libraries (25 µL) were purified using Agentcourt AMPure XP beads (15 µL, ratiobeads/dsDNA=0.6, Beckman Coulter) to remove impurities and obtain high-quality libraries ...
-
bioRxiv - Cell Biology 2023Quote: ... of proteins were applied on the top of a 16 ml 10-30% continuous sucrose density gradient and centrifuged at 74,400 g for 15 h at 4 °C (SW 32.1 Ti; Beckman Coulter). 24 equal volume fractions were collected ...
-
bioRxiv - Biophysics 2020Quote: ... lysate was centrifuged at 5 °C with 13000 rpm for 15 min by Avanti J-E Ultra Centrifuge (Beckman Coulter). Supernatant from centrifugation was filtered with 0.45 μm SFCA syringe filters (Sigma Aldrich ...
-
bioRxiv - Biophysics 2022Quote: ... The cell suspension was centrifuged for 5 min at 2000 g (Centrifuge Beckman Coulter Allegra X-15-R, Rotor Sx4750A)) and 4°C ...
-
bioRxiv - Microbiology 2019Quote: ... The bacterial cells were recovered by centrifugation (in a JLA 81000 rotor, Beckman-Coulter, at 6 Krpm for 15 min) and disrupted by sonication (VibraCell 75042 sonicator ...
-
bioRxiv - Cell Biology 2019Quote: ... all proteins were pre-cleared of aggregates via centrifugation at 80,000 RPM for 15 min at 4°C in a TLA100 rotor (Beckman Coulter).
-
bioRxiv - Biochemistry 2019Quote: ... Reactions were spun over 15-30% sucrose gradients made in buffer C with appropriate MgCl2 concentrations at 178,000 g (SW-41 rotor, Beckman Coulter) for 3 hours ...
-
bioRxiv - Biochemistry 2021Quote: ... 500 μl M2 eluate was added to the top of a 12 ml 15 - 35 % glycerol gradient and centrifuged in an SW40Ti rotor (Beckman) at 40 ...
-
bioRxiv - Plant Biology 2020Quote: ... The sucrose gradient was centrifuged at 154,300 × g for 15 h at 4 °C using a SW 41 Ti rotor (Beckman Coulter). The LHCII proteoliposomes separated from empty liposomes were collected dropwise from the bottom of the tube and used for analysis.
-
bioRxiv - Plant Biology 2021Quote: ... The plant debris was removed by centrifuging at 16 000 g for 15 minutes at 4°C in a JA-25.50 rotor and Avanti J- 20 XP centrifuge (Beckman Coulter). The clear supernatant was then gently poured on top of 8 ml sucrose cushion (100 mM Tris·HCl (pH 8.0) ...
-
bioRxiv - Microbiology 2021Quote: ... bacteria were harvested by centrifugation at 4000 x g for 15 min (Microfuge 20R; Beckman Coulter, La Brea, CA, USA). The pellet was washed twice in PBS ...
-
bioRxiv - Cell Biology 2020Quote: ... cells and larger debris were removed by 2000 × g centrifugation of cell suspensions for 15 minutes (Rotor: JS-5.3; Beckman Coulter). MSC-free CM were stored at −20°C until usage ...
-
bioRxiv - Biochemistry 2022Quote: ... 40 μl of the mixture was transferred to an ultracentrifuge tube and centrifuged at around 100,000 x g for 15 minutes at room temperature using the Airfuge (Beckman Coulter). After centrifugation ...
-
bioRxiv - Microbiology 2022Quote: ... and layered onto a preformed 11 mL 5% to 15% (wt/vol) Ficoll gradient in a 13.2 mL thin-wall polyallomer ultracentrifuge tube (Beckman Coulter). Gradients were centrifuged at 12,000 rpm for 2 h at 4°C in an SW41 Ti swinging-bucket rotor in a Sorvall Discovery SE ultracentrifuge ...
-
bioRxiv - Biophysics 2022Quote: T1ST3SC12-(Ndc80)x modules were separated from Ndc80 on a 15-50% glycerol gradient of approximately 12 ml in a SW40 rotor (Beckman) for 16 hrs at 4 °C ...
-
bioRxiv - Cell Biology 2020Quote: ... Cleared supernatants obtained from cytoplasmic lysates were loaded on a linear 15%-50% sucrose gradient and ultracentrifuged in a SW41Ti rotor (Beckman) for 1 h and 40 min at 180,000 x g at 4°C in a Beckman Optima LE-80K Ultracentrifuge ...
-
bioRxiv - Microbiology 2019Quote: ... including library amplification for 15 PCR cycles using custom indexed primers and post-PCR clean-up with 0.85x volume Ampure XP (Beckman Coulter). Libraries were quantified using Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen ...
-
bioRxiv - Cell Biology 2020Quote: ... This crude mitochondrial pellet was further purified on a discontinuous percoll gradient (buffer composition 15/23/40% sucrose) by centrifugation (40,000 x g, 1hr, 4°C) in an SW41 rotor (Beckman Coulter) and enriched mitochondria were collected from the 23-40% interface (adapted from [76]) ...
-
bioRxiv - Neuroscience 2022Quote: ... AAV was purified from the resulting lysate using an iodixanol step gradient containing 15, 25, 40 and 60% iodixanol (Batool et al., 2021) in optiseal tubes (Beckman) followed by centrifugation at 350,000 xg using a Type 70 Ti ultracentrifuge rotor (Beckman) ...
-
bioRxiv - Molecular Biology 2022Quote: ... and centrifuged at 15 000 RPM for 1 minute using an AllegraTM X-22R Centriuge (Beckman Coulter™, California, USA) to ensure all reagents were concentrated at the bottom of the wells ...
-
bioRxiv - Cell Biology 2022Quote: ... 40 μl of the mixture was then transferred to an ultracentrifuge tube and centrifuged at around 100,000 g for 15 minutes at room temperature using an airfuge (Beckman Coulter). After the centrifugation ...
-
bioRxiv - Biophysics 2023Quote: ... Reactions were centrifuged at 400,000 g at 20 °C for 15 min in polycarbonate centrifuge tubes (Beckman Coulter Inc. USA). The pellets were resuspended in 40 μl reaction buffer ...
-
bioRxiv - Genomics 2023Quote: ... The ribosomes were further purified by centrifugation at 59,727 ×g (15 hours, 4 °C, SW 32.1 Ti rotor, Optima™ L-100 XP, Beckman Coulter) in a linear (15 – 30 % ...
-
bioRxiv - Neuroscience 2023Quote: ... the crude lysate was subjected to iodixanol gradient (15%, 25%, 40% and 60%) ultracentrifugation using a Type 70Ti rotor (Beckman) at 490000 × g ...