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Citations for Beckman :
1 - 50 of 199 citations for Siglec 15 Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: Interstitial macrophages (CD45+CD64+Siglec-F−CD11bhigh) or alveolar macrophages (CD45+CD64+Siglec-F+CD11blow) were isolated from C57BL/6 by MOFLO system (Beckman Coulter). Isolated macrophages were cultured for 24 hrs in RPMI-1640 medium containing 10% FBS and 50μM 2-mercaptoethanol with or without GM-CSF (10 ng/ml).
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bioRxiv - Bioengineering 2021Quote: ... or using flow cytometry (HEK293, CytoFlex, Beckman Coulter).
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bioRxiv - Genomics 2019Quote: ... the Hi-C libraries were purified with AMPure XP beads (Beckman Coulter). The concentration of the Hi-C libraries was determined by Bioanalyzer profiles (Agilent Technologies) ...
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bioRxiv - Cancer Biology 2021Quote: ... the Hi-C libraries were purified with AMPure XP beads (Beckman Coulter). Quantity and integrity of the Hi-C libraries was determined by Bioanalyzer profiles (Agilent Technologies).
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bioRxiv - Biochemistry 2020Quote: ... 15 min (Beckman J2-HC Centrifuge). Pellets were transferred to a 50 mL polypropylene Falcon tube and weighed ...
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bioRxiv - Systems Biology 2021Quote: HeLa Kyoto and R1/E mESCs (gifts from Hyman lab, Dresden) and HEK293 (gift from Beckman lab, Berlin), Ptk2 cell lines (gift from Simons lab ...
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bioRxiv - Genetics 2019Quote: ... The Hi-C libraries were purified with AMPure XP beads (Beckman Coulter, A63881) and sequenced using an Illumina sequencing platform.
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bioRxiv - Biochemistry 2020Quote: ... for 15 min at 4°C (Beckman centrifuge J-20-XP ...
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bioRxiv - Biochemistry 2020Quote: ... for 15 min at 4°C (Beckman centrifuge J-20-XP ...
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bioRxiv - Biochemistry 2020Quote: ... for 15 min at 4°C (Beckman centrifuge J-20-XP ...
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bioRxiv - Genetics 2022Quote: ... and the final Hi-C library was purified with AMPure XP beads (Beckman Coulter). Promoter Capture Hi-C was performed using a customdesign Agilent SureSelect system following the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: Infectivity and neutralization titers were determined using ACE2-expressing HEK293 target cells (Integral Molecular) in a semi-automated assay format using robotic liquid handling (Biomek NXp Beckman Coulter). Test sera were diluted 1:40 in growth medium and serially diluted ...
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bioRxiv - Genomics 2023Quote: ... and Hi-C template DNA was purified using 1x AMPure XP beads (Beckman Coulter, A63881) and stored at 4°C until library preparation ...
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Potential for virus endogenization in humans through testicular germ cell infection: the case of HIVbioRxiv - Microbiology 2020Quote: ... CD45-PC7 Ab (clone J33, 1/15, Beckman Coulter), CD3-BV510 Ab (clone HIT3a ...
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bioRxiv - Cell Biology 2019Quote: ... Fluorescence was measured on a Cytoflex 15 (Beckman Coulter) and 10,000 cells per sample were analyzed ...
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bioRxiv - Molecular Biology 2019Quote: ... Clarified extracts were prepared by incubating cells with lysis buffer for 15 min on ice followed by centrifugation for 15 min at 16,162 rcf (Beckman Coulter, Microfuge® 16). Extract concentration was quantified by bicinchoninic acid assay (Pierce #23225 ...
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bioRxiv - Immunology 2021Quote: ... Infectivity and neutralization titers were determined using ACE2-expressing HEK293 target cells (Integral Molecular, Philadelphia, PA) in a semi-automated assay format using robotic liquid handling (Biomek NXp Beckman Coulter, Brea, CA). Virions pseudotyped with the vesicular stomatitis virus (VSV ...
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bioRxiv - Microbiology 2022Quote: ... Infectivity and neutralization titers were determined using ACE2-expressing HEK293 target cells (Integral Molecular, Philadelphia, PA) in a semi-automated assay format using robotic liquid handling (Biomek NXp Beckman Coulter, Brea, CA). Virions pseudotyped with the vesicular stomatitis virus (VSV ...
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bioRxiv - Biochemistry 2019Quote: ... 10 mM Tris:HCl pH 7.5) and resolved on a 10–40% sucrose gradient in overlay buffer in a zonal rotor (Ti 15, Beckman, 15 h at 21,000 rpm). The peak containing 80S ribosomes was pelleted by centrifugation (19 h at 40,000 rpm ...
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bioRxiv - Biochemistry 2022Quote: ... 4°C for 15 min using a JLA-9.1000 centrifuge rotor (Beckman). The pellet was resuspended in 70 mL buffer containing 50 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2022Quote: ... Single-cell clones were then assessed for binding to His-tagged SARS-CoV-2 Spike using a Cytoflex S (Beckman) cell analyser ...
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bioRxiv - Cell Biology 2021Quote: ... The homogenized solution was centrifuged at 75,000 rpm for 15 min (Beckman, TL100.3). The supernatant was loaded onto a StrepTactin Sepharose column with a volume of 1 mL ...
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bioRxiv - Genomics 2019Quote: ... and using 15 cycles of PCR followed by an Ampure XP bead (Beckman) clean-up.
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bioRxiv - Cell Biology 2023Quote: ... continuous 15-45 % (w/v) sucrose gradients were prepared in SW41 tubes (Beckman) in Polysome Gradient Buffer (10 mM Hepes-KOH ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were loaded on 15-50% sucrose density gradients and centrifuged (Beckman ultracentrifuge) for 1 hour and 30 minutes in a SW41Ti swinging bucket rotor at 39000 rpm at 4 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... Purified cDNA was amplified for 15 cycles and cleaned up using SPRIselect beads (Beckman). Samples were quantified on an Invitrogen Qubit 4 Fluorometer ...
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bioRxiv - Cell Biology 2020Quote: ... for 15 min at 37°C before analysis using CytoFLEX (Beckman Coulter Life Science). Treatment with 10 µM valinomycin for 30 min was included prior to JC-1 incubation as a depolarising control ...
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bioRxiv - Genomics 2020Quote: ... Purified cDNA was amplified for 15 cycles before bead cleanup using SPRIselect beads (Beckman). Samples were quantified using an Invitrogen Qubit 4 Fluorometer ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were harvested by centrifugation for 15 minutes in a JLA-9.1000 rotor (Beckman) at 5,180 x g and the pellets were stored at –20 °C.
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bioRxiv - Cell Biology 2019Quote: ... for 15 minutes at room temperature and analyzed with flow cytometry (Beckman Coulter, Cytoflex). Alternatively ...
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bioRxiv - Molecular Biology 2022Quote: ... After centrifugation at 6000 g for 15 min at 4 °C (JA14 rotor-Beckman), the cell pellet is resuspended with cold lysis buffer (1mg/ml lysozyme ...
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bioRxiv - Genetics 2021Quote: ... 15-60% sucrose gradients were prepared in 89 mm polypropylene centrifuge tubes (Beckman Coulter) using standard settings on a Foxy Jr ...
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bioRxiv - Immunology 2019Quote: ... Lymphocyte counts (4-15 μm) were determined with a Z2 Coulter Counter (Beckman Coulter). For surface staining ...
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bioRxiv - Microbiology 2021Quote: ... and centrifuged each time at 4000 × g for 15 min (Microfuge 20R, Beckman Coulter) followed by resuspension in PBS.
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bioRxiv - Genomics 2023Quote: ... the cDNA was cleaned up and eluted into 15 µL using AMPure beads (Beckman) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: A 15-45% continuous sucrose gradient was made in Thinwall polyallomer tubes (Beckman Coulter) from 15% and 45% sucrose (MP Biomedicals ...
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bioRxiv - Biochemistry 2021Quote: ... The bacterial culture was centrifuged at 4,000 × g for 15 min at 4°C (Beckman) and the cell pellet was resuspended in 30-40 mL of lysis buffer (50 mM HEPES pH 8.0 ...
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bioRxiv - Immunology 2021Quote: ... 5000 rpm for 15 min using the Avanti® J-26xp centrifuge (Beckman Coulter®) and resuspended in lysis buffer composed of 50 mM Tris/HCl pH 8 ...
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bioRxiv - Cell Biology 2023Quote: ... The lysate was centrifuged at 4 °C for 15 min in a TLA55 rotor (Beckman) at 50,000 rpm to remove insoluble material ...
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bioRxiv - Biochemistry 2022Quote: ... treatment for 15 min at 37°C on an automated Biomek i7 Hybrid system (Beckman Coulter). RNA quantification and quality control was performed on Fragment Analyzer 5300 system and showed an RNA integrity number (RIN ...
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bioRxiv - Microbiology 2021Quote: ... followed by centrifugation for 15 min (14000 x g) at 4 °C (Beckman Coulter, Inc USA). The aqueous phase was transferred to a fresh 1.5 ml tube and the organic phase was kept for deoxyribonucleic acid (DNA ...
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bioRxiv - Biochemistry 2023Quote: ... 0.5 mM EDTA) at 42,800 x g for 15 h using a SW28 rotor (Beckman Centrifuge). Gradients were fractionated using an AKTA system and the 80S ribosome fractions pooled ...
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bioRxiv - Molecular Biology 2023Quote: ... and the 70S ribosomes were isolated by zonal centrifugation in a Ti 15 rotor (Beckman Coulter) for 15 h at 28,000 rpm on a sucrose gradient from 10 % to 40 % in buffer X ...
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bioRxiv - Cell Biology 2023Quote: ... IgG2a Mouse-PE (A09142, Beckman Coulter), IgG1κ Mouse-AF647 (565571 BD Biosciences) ...
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bioRxiv - Molecular Biology 2021Quote: ... After two short clearance spins (Type 45 Ti rotor from Beckman Coulter, 15 min at 20000 rpm), lysates were spun for 24 h at 4°C at 50000 rpm in a Type 70 Ti rotor (Beckman Coulter ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNAs were amplified by 15 cycles of PCR reactions and subsequently purified by AMpure XP beads (Beckman). Each individual library was quantified ...
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bioRxiv - Molecular Biology 2021Quote: ... Following ultra-centrifugation (100,000 x g; 4°C; 15 min; rotor TL-100; OptimaMax-XP, Beckman-Coulter), solubilized membrane protein samples (20μl extract with BN-PAGE loading buffer ...
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bioRxiv - Biochemistry 2020Quote: ... 20 mM Tris:HCl pH 7.5) in a zonal rotor (Ti 15, Beckman, 17 hours at 21,000 rpm). The peak containing pure 70S ribosomes was pelleted by centrifugation (20 hours at 35,000 rpm) ...
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bioRxiv - Cell Biology 2019Quote: ... seeds were diluted in BRB80 and span for 15 min at 100,000 g (Optima TL100; Beckman Coulter). Sedimented MT seeds were resuspended in 60 μl of BRB80 ...
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bioRxiv - Microbiology 2020Quote: ... Ribosomes were harvested by centrifugation at 40,000 rpm for 15 h in an SW40Ti rotor (Beckman Coulter). To isolate the ribosome-bound mRNAs or ribosome footprints (RPF) ...