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Citations for Beckman :
151 - 200 of 422 citations for SARS CoV 2 Spike Glycoprotein S2 aa 800 1000 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Centrifugation was performed at 120,000 × g for 2 hr (Beckman TLS 55 rotor), after which the interface between 0.25 M and 1.1 M sucrose was collected and resuspended in 1 ml 19% OptiPrep for a step gradient containing 0.5 ml 22.5% ...
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bioRxiv - Cell Biology 2022Quote: ... 2× – G2) was interrogated using a using a Gallios flow cytometer (Beckman Coulter) and subsequent data analysis with FlowJo (v.10.8.0) ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were centrifuged at 1500 rpm for 2 minutes (Beckman Allegra X15R, 524xg), supernatant was removed and tissue was carefully triturated using fire-polished glass pipettes with 2 mL pre-warmed media ...
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bioRxiv - Genomics 2019Quote: ... Tagmented DNA was purified using 2× SPRI beads (Agencourt AMPure XP-Beckman Coulter). Finally ...
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bioRxiv - Synthetic Biology 2021Quote: PCR products were purified with 2 volumes of RNAClean XP beads (Beckman Coulter) and then quality-checked and quantified on a Bioanalyzer (Agilent) ...
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bioRxiv - Biophysics 2022Quote: ... 2 mM DTT) with 0.7 M sucrose in Ti-45 tubes (Beckman-Coulter). The tubes were then placed in a Ti-45 rotor and spun at 27,000 rpm (57,000 xg ...
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bioRxiv - Biophysics 2022Quote: ... 2 M urea) by resuspension and centrifugation on a JS-4.2 rotor (Beckman) at 6,000 RPM for 30 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... for 2 h using 25 ξ 89 mm polycarbonate tubes (Beckman Coulter, 355618). Pellets were resuspended in 500 μL of sterile phosphate-buffered saline (PBS ...
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bioRxiv - Neuroscience 2023Quote: ... for 2 h using 16 ξ 76 mm polycarbonate tubes (Beckman Coulter, 355603). The resulting pellets were resuspended in 250 μL of sterile PBS ...
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bioRxiv - Synthetic Biology 2023Quote: ... cells were diluted 2-fold with PBS and analysed with CytoFLEX S (Beckman Coulter ...
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bioRxiv - Molecular Biology 2023Quote: ... The ribodepleted RNA was cleaned using 2× RNAClean XP beads (Beckman Coulter A63987), eluting with 20 μL DNase digestion reaction mixture (1.5 μL Turbo DNase (Invitrogen AM2238) ...
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bioRxiv - Immunology 2024Quote: ... and 2 mM DTT) and separated by ultracentrifugation at 148,900xg (Ti55 rotor, Beckman) for 1.5 hours at 4°C ...
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bioRxiv - Genetics 2024Quote: ... DNA was further purified using 2 volumes of Ampure XP beads (Beckman Coulter). DNA was extracted from 10000 undifferentiated or differentiated spermatogonia cell samples by QIAamp DNA Micro Kit (Qiagen # 56304 ...
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bioRxiv - Cancer Biology 2021Quote: ... Purification of the library for optimal library preparation included removal of primer dimers and DNA fragments larger than 1000 base pairs (bp) through double-sided bead purification with Agencourt AMPure XP beads (Beckman Coulter). Sequencing of the final library was performed by GENEWIZ using the Illumina HiSeq 2500 system.
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bioRxiv - Neuroscience 2021Quote: ... 1 ml of the supernatants from the 1000 x g centrifugation were laid on top of the gradient and centrifuged at 200,000 x g (Beckman SW40 rotor) for 4 h ...
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bioRxiv - Biochemistry 2020Quote: ... and pelleted for 3 min in a swing-out rotor at 1000 rpm in a tabletop centrifuge (Allegra X-30R, Beckman Coulter). The supernatant was aspirated and cells were suspended in the remaining liquid to 1 × 105 – 1 × 107 cells/100 μl ...
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bioRxiv - Immunology 2019Quote: ... CECs were pre-incubated with human recombinant CCL5 (1-1000 ng/ml, R&D) and/or CD35 blocking antibody (5-20 μg/ml, clone J3D3, Beckman Coulter) for an hour prior to infection.
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bioRxiv - Biochemistry 2021Quote: ... live cells were stained with Alexa 647-conjugated M1 anti-FLAG antibody (1:1000, 5 minutes) and analyzed on a Cytoflex flow cytometer (Beckman Coulter).
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bioRxiv - Cancer Biology 2021Quote: ... decrosslinked material was purified with 2 x AMPure XP beads (Beckman Coulter, Cat #A63881) and eluted in 30 μL of 10 mM Tris-Cl ...
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bioRxiv - Molecular Biology 2020Quote: ... and centrifuged for 2 hours at 55,000 rpm (TLS-55 rotor, Beckman Coulter, USA). 11x 200 μl fractions were harvested and subjected to FLAG-IP ...
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bioRxiv - Genomics 2019Quote: ... The reaction was next cleared by 2× AmpureXP beads (Agencourt AMPure XP, Beckman Coulter) and eluted in 75 μl EB (10 mM Tris-HCl pH 8) ...
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bioRxiv - Cancer Biology 2021Quote: ... resuspended in 300μl PBS/2%FBS and FACS analysed on Gallios® (Beckman Coulter) or ARIAIII (BD) ...
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bioRxiv - Physiology 2019Quote: ... RNA was then cleaned using 2 volumes of Agencourt RNAClean XP (Beckman Coulter, A63987) beads under the standard protocol ...
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bioRxiv - Biophysics 2023Quote: ... 2 mM DTT) with 0.7 M sucrose in Type 45 Ti tubes (Beckman-Coulter). Ribosomes were pelleted by centrifugation at 27,000 rpm (57,000 xg ...
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bioRxiv - Cancer Biology 2023Quote: ... concentrated for 2 h by ultracentrifugation in swing-out rotor SW-32Ti (Beckman Coulter), 26,000g ...
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bioRxiv - Physiology 2023Quote: ... Samples were ultracentrifuged at 100,000 rpm at 16°C for 2 hours (rotor: Beckman TLA-100 ...
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bioRxiv - Plant Biology 2023Quote: ... followed by ultracentrifugation at 4°C for 2 h (38000 rpm, Beckman, SW41 rotor) through a 20-60% sucrose gradient (40 mM Tris-HCl pH 8.4 ...
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bioRxiv - Systems Biology 2023Quote: ... Adapter ligation was followed by 2 successive Agencourt AMPure XP bead purifications (Beckman Coulter) and library amplification by a Plasmodium-optimized KAPA PCR amplication protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... and ultra-centrifuged for 2 hours at 20,000 rpm in an SW28 rotor (Beckman). Viral pellets were resuspended in 250 µL PBS and flash-frozen ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Illumina RNASeq sequencing (HiSeq 2000, 2 × 75 pb) and trimming were performed by Beckman Coulter Genomics ...
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bioRxiv - Synthetic Biology 2021Quote: ... 72C for 2’) and a X0.65 SPRI bead DNA clean up (AMPure XP, Beckman Coulter) a second PCR using a p5 primer (F6 ...
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bioRxiv - Systems Biology 2022Quote: ... Final libraries were size-selected by performing 2-step bead cleaning (SPRIselect, Beckman Coulter B23318) to remove larger DNA fragments and primer dimers and quality was assessed by Bioanalyzer (Agilent 2100 ...
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bioRxiv - Biochemistry 2019Quote: ... After ultra-centrifugation (SW-60Ti rotor Beckman Coulter, 2 h, 100 000 rpm, 4°C) the gradient was manually fractioned in 200 μl fractions from the top to the bottom of the tube ...
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bioRxiv - Genomics 2022Quote: ... then ultracentrifuge at 24,000 rpm for 2 h using SW28 swinging bucket rotor (Beckman Coulter). Upon completion ...
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bioRxiv - Microbiology 2022Quote: ... The filtrate was then ultracentrifuged 100,000 g for 2 hours (Optima L-90K, Beckman Coulter). The vesicle pellet was resuspended in 100 μl of sterile PBS.
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bioRxiv - Genomics 2023Quote: ... then ultracentrifuge at 24,000 rpm for 2 h using SW28 swinging bucket rotor (Beckman Coulter). Upon completion ...
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bioRxiv - Bioengineering 2021Quote: ... The tubes were then centrifuged at 1000 g for 15 minutes at 4°C using a Microfuge™ 22R (Beckman Coulter, Brea, CA), and the serum was then stored at -80°C ...
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bioRxiv - Cell Biology 2022Quote: ... The product from PCR-2 was purified using 1x volume of AMPure XP beads (Beckman Coulter) and eluted in 15 μL of IDTE buffer (IDT DNA) ...
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bioRxiv - Cell Biology 2021Quote: ... After centrifugation (285, 000 g for 2 h at 4 °C using a Beckman SW40Ti rotor), polysome profiles were recorded using an Äktaprime plus chromatography system (GE Healthcare) ...
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bioRxiv - Bioengineering 2021Quote: ... The supernatant was then ultracentrifuged for 2 hours using Optima™ L-80XP Ultracentrifuge (Beckman Coulter) at 100,000 x g at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... The solution was centrifuged at 36,000 rpm for 2 h in a SW41Ti rotor (Beckman Coulter) at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... 4°C for 2 h (SW 32 Ti swinging-bucket rotor, Optima LE-80K Beckman Coulter) using a 25% w/v sucrose cushion ...
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bioRxiv - Genetics 2021Quote: ... We purified the final libraries with 2 rounds of 0.8X volume of SPRISelect (Beckman Coulter, B23318).
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bioRxiv - Immunology 2020Quote: ... were incubated with 1-2 million 1D8 cells following standard FACS protocol and analyzed by Beckman.
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bioRxiv - Microbiology 2021Quote: ... and centrifuged for 2 h at 64,000 g in A27-8×50 mL rotors (Beckman Coulter) to sediment virus particles ...
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Dynamic states of eIF6 and SDS variants modulate interactions with uL14 of the 60S ribosomal subunitbioRxiv - Biochemistry 2022Quote: ... Gradients were centrifuged at 35,000 rpm for 2 hrs 40 min using a SW41Ti rotor (Beckman). Gradients were fractionated using a Brandel (UV ...
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bioRxiv - Microbiology 2023Quote: ... Three 1:2 serial dilutions were done suing a Biomek i7 liquid handler (Beckman Coulter, USA), in order to obtain the four working concentrations ...
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bioRxiv - Cell Biology 2022Quote: ... Equal volume (2 ml i.e 1600 µg) of cell extracts were loaded in ultracentrifuge tubes (Beckman). Samples were ultracentrifuged at 100,000 g for 30 minutes at 4°C in a Optima XPN-100 ultracentrifuge (Beckman) ...
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bioRxiv - Bioengineering 2023Quote: ... in 2%BSA and 2mM EDTA and sorted using FACS sorter (Beckman Coulter, MoFlo Astrios Zellsortierer). For gating ...
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bioRxiv - Immunology 2023Quote: Cells were washed with 2% FACs and data was acquired using CytoFLEX S (Beckman Coulter, USA) and analyzed via FlowJo v10 software (Oregon ...