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Citations for Beckman :
101 - 150 of 422 citations for SARS CoV 2 Spike Glycoprotein S2 aa 800 1000 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2019Quote: ... then centrifuged at 5000 x g and 10°C for 10 minutes (Avanti□ J-E Centrifuge, Beckman Coulter, Indianapolis, IN). After disposing the supernatant ...
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bioRxiv - Biochemistry 2022Quote: ... A soluble fraction was obtained after centrifugation of the lysate at 19,000 rpm for 40 min at 4 °C performed using Avanti J-E with a rotor JA-20 (BECKMAN COULTER) to remove insoluble debris.
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bioRxiv - Plant Biology 2024Quote: ... The mixture was then centrifuged for 20 min at 9,700 g at 6°C using an Avanti J-E centrifuge and a JLA-16.250 rotor (Beckman Coulter, USA). The supernatant was separated and centrifuged for 2.5 h at 200,000 g at 6°C using an Optima XPN-90 ultracentrifuge and a Type 45 Ti rotor (Beckman Coulter ...
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bioRxiv - Plant Biology 2024Quote: ... The suspension was then centrifuged for 20 min at 9,700 g at 6°C using an Avanti J-E centrifuge and a JLA-16.250 rotor (Beckman Coulter, USA). The supernatant was separated and centrifuged for 3 h at 200,000 g at 6°C using an Optima XPN-90 ultracentrifuge with a Type 45 Ti rotor (Beckman Coulter ...
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bioRxiv - Microbiology 2021Quote: ... Samples were diluted to a DNA concentration of 50 ng/µl followed by ligation of 454 sequencing adaptors and molecular identifier (MID) tags using the SPRIworks Fragment Library System II for Roche GS FLX+ DNA Sequencer (Beckman Coulter), excluding fragments smaller than 350 base pairs ...
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bioRxiv - Immunology 2022Quote: ... Targeted amplification was performed for 13 cycles and the products were separated according to size into <400 bp (DNA barcode-tags) and >400 bp (the TCR sequences) using 0.6x SPRIselect beads (Beckman Coulter, B23318). Separate processing of the >400 bp bead-bound TCR sequences and the <400 bp in solution DNA barcodes was conducted according to manufacturer’s instruction and the TCR amplification products were sequenced on a NovaSeq running a 150 paired-end program ...
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bioRxiv - Genetics 2022Quote: ... uric acid and urea were measured on a UniCel® DxC 800 Synchron® Clinical System or AU480 Clinical Chemistry System (Beckman Coulter, Brea, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: A 3 ml volume of pseudoparticles was pelleted using a TLA-55 rotor with an Optima-MAX-E ultracentrifuge (Beckman Coulter) for 2 hours at 42,000 rpm at 4°C ...
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bioRxiv - Plant Biology 2019Quote: ... The corresponding TAG spots were scraped and quantified for radioactivity using a LS 6500 multi-purpose scintillation counter (Beckman-Coulter, Mississauga, Canada).
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bioRxiv - Cell Biology 2021Quote: ... coli cells obtained from 500 mL culture were harvested using centrifugation for 10 min at 4800 rpm (Beckman, Allegra-30XR), and were subjected to freezing in liquid nitrogen ...
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bioRxiv - Microbiology 2022Quote: ... coli treated cells were also diluted with PBS and sorted using a MoFlo XDP High-Speed Cell Sorter (Beckman Coulter) equipped with 405 nm ...
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bioRxiv - Microbiology 2020Quote: ... and the resultant lysate was transferred into SS34 tubes for further centrifugation at 18,000 rpm (JA-25.50 rotor, Beckman Coulter Avanti J-E) for 30 min ...
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bioRxiv - Cancer Biology 2022Quote: ... and then concentrated by ∼1000 fold by ultracentrifugation (Beckman Coulter). The titre was quantified by FACS of LSL-tdTomato mouse embryonic fibroblasts (MEFs).
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bioRxiv - Immunology 2022Quote: ... and mRNA PCR products were separated from sample tag and AbSeq products with double-sided size selection using Agentcourt® AMPure® XP magnetic beads (Beckman Coulter, #A63880). mRNA and Sample Tag products were further amplified ...
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bioRxiv - Biophysics 2023Quote: ... Cell debris was removed by centrifugation at 20,000 rpm at 4°C for 1 h (Avanti® J-E centrifuge from Beckman Coulter, rotor: JLA 25.50). The supernatant was collected and loaded onto a pre-equilibrated 5 ml His-Trap FF crude column ...
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bioRxiv - Biochemistry 2023Quote: ... Pellets were washed and gently resuspended in 1 ml of PBS and centrifuged at 50,000 × g for 1 h at 4°C (Beckman Optima MAX-E, TLA-55 rotor). The resulting pellets were resuspended in 500 μl of PBS to obtain concentrated lentiviral stocks that were stored at −80°C in aliquots ...
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bioRxiv - Biophysics 2023Quote: ... Cells were harvested by centrifugation at 8000 g at 4°C for 20 min (Avanti® J-E centrifuge from Beckman Coulter, rotor: JLA 16.250).
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bioRxiv - Bioengineering 2023Quote: ... subtilis spores were collected from the culture by the centrifugation at 8000 rpm at 4 °C for 20 min (Avanti J-E, Beckman Coulter Life Science, IN, USA). Supernatant was removed and the spore pellets were resuspended in fresh 100 mM PBS pH 7.4 ...
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bioRxiv - Biochemistry 2024Quote: ... Pellets were washed and gently resuspended in 1 mL of PBS and centrifuged at 50,000 x g for 1 h at 4°C (Beckman Optima MAX-E, TLA-55 rotor). Pellets were resuspended in 500 μl of PBS to obtain concentrated lentiviral stocks that were stored at −80°C in aliquots ...
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bioRxiv - Neuroscience 2024Quote: ... 20.000 rpm for 2 hours (Beckman). Collected viral particles were then suspended in DMEM and stored to –80°C ...
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bioRxiv - Cell Biology 2021Quote: ... Beads were centrifugated at 1000 g for 5 min at 4°C (Beckman Coulter Microfuge 22R ...
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bioRxiv - Cell Biology 2022Quote: ... and the post-nuclear supernatant was loaded onto an 8-step discontinuous sucrose density gradient (HEPES-buffered 0.2–2 M sucrose) and centrifuged at 55,000 rpm for 2h at 4 °C using an MLS50 rotor (Beckman Coulter). Extracellular acidification rate (ECAR ...
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bioRxiv - Microbiology 2021Quote: ... using anti-HIV-1 p24-PE antibody (#6604667, 1:1000 dilution) purchased from Beckman Coulter ...
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bioRxiv - Genomics 2020Quote: ... and then large fragments (above 1000 bp) removed with 1X AMPure beads (Beckman Coulter). The concentration of libraries was measured with Qubit dsDNA HS Assay (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... Prepared DNA libraries were size-selected (150-1000 bp) by Ampure XP (Beckman Coulter). Sequencing was performed using NextSeq500 (Illumina).
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bioRxiv - Immunology 2019Quote: ... Receptor (R; Beckman Coulter, Z199, GL183, CD158e1/2) and KIR2DS4 (S4 ...
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bioRxiv - Biochemistry 2021Quote: ... 2 mM DTT) and centrifuged for 2 hours and 15 minutes at 39000 rpm at 4°C (Beckman Coulter TLS55 rotor). Fractions corresponding to the large mitochondrial subunit were collected and subjected to buffer exchange (25 mM HEPES-KOH pH = 7.5 ...
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bioRxiv - Developmental Biology 2020Quote: ... The fragments ranging 200– 1000 bp were size-selected using AMPure beads (Beckman Coulter, #A63881) (first round 0.5x beads ...
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bioRxiv - Genomics 2019Quote: ... The fragments ranging 200 - 1000 bp were size-selected using AMPure beads (Beckman Coulter, #A63881) (1st round 0.5X beads ...
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bioRxiv - Microbiology 2021Quote: ... calibrating with 2-micron latex bead standard (Beckman Coulter) diluted in 18% BSW as the electrolyte ...
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bioRxiv - Genomics 2021Quote: ... then size-selected for fragments between 150 and 1000 bp using SPRISelect beads (Beckman Coulter, B23319). Libraries were sent for single-end sequencing on the HiSeq 4000 (50 bp single-end reads) ...
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bioRxiv - Developmental Biology 2021Quote: ... inhibin B and AMH/MIS by 2-site ELISA (Beckman Coulter and Beckman Coulter Gen II ...
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bioRxiv - Immunology 2022Quote: ... Tagmented DNA was isolated using 2× SPRI Beads (Beckman Coulter) and amplified via PCR ...
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bioRxiv - Biochemistry 2020Quote: ... Fragments were purified using 2 volumes AMPure XP beads (Beckman Coulter) and amplified for 8-10 cycles using NEBNext Multiplex Oligos ...
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bioRxiv - Biochemistry 2021Quote: ... 6 mM 2-mercaptoethanol and centrifuged with a 70Ti (Beckman coulter) rotor at 40,000 rpm for 18 hours at 4°C ...
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bioRxiv - Genomics 2022Quote: ... and purified with 2 rounds of AMPure XP beads (Beckman Coulter). Libraries were sequenced on an Illumina NextSeq 550 ...
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bioRxiv - Biochemistry 2020Quote: ... Fragments were purified using 2 volumes AMPure XP beads (Beckman Coulter) and amplified for 8-10 cycles using NEBNext Multiplex Oligos ...
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bioRxiv - Genomics 2023Quote: ... prepare sucrose gradient in 2 ultracentrifuge buckets (Beckman Coulter cat: 344058) by layering 5 mL of Interphase buffer atop 10 mL of SCB in each ...
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bioRxiv - Molecular Biology 2019Quote: ... the VLPs were pelleted for 2 h at 40,000 rpm (Beckman TLA55) at 4°C and subsequently resuspended in PBS (pH 7.4) ...
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bioRxiv - Molecular Biology 2019Quote: ... 2 mM DTT) with 0.7M sucrose in ti-45 tubes (Beckman-Coulter). Ribosomes were pelleted in a Ti-45 rotor at 27000 rpm for 16 hours at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... using an An50Ti rotor and standard 2-channel epon centerpieces (Beckman-Coulter). All the samples were measured in the same run and at multiple wavelengths (278 nm ...
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bioRxiv - Biophysics 2020Quote: ... VLPs were pelleted for 2 h at 40,000 rpm (Beckman TLA55 rotor) at 4°C and solved in PBS (pH 7.4).
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bioRxiv - Genomics 2021Quote: ... Libraries were purified with 2 rounds of Ampure XP beads (Beckman-Coulter) and evaluated on a 2100 Bioanalyzer (Agilent) ...
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bioRxiv - Biochemistry 2021Quote: ... using an An50Ti rotor and standard 2-channel epon centerpieces (Beckman-Coulter). All samples were measured in the same run ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 μL of the compounds were added automatically (Beckman FX 96 tip) to 200 μL media to make it up to the final concentration for each drug ...
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bioRxiv - Immunology 2020Quote: ... After adding 2 mL of VersaLyse solution (Beckman Coulter, catalog number A09777) with 2.5% Fixative Solution (Beckman Coulter ...
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bioRxiv - Immunology 2022Quote: ... all from BD Biosciences as well as IL-2 PE (clone N7.48A, Beckman, Cat IM2718U) and TNF PCP-Cy5.5 (clone MAb11 ...
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bioRxiv - Biochemistry 2024Quote: ... using an An60Ti rotor and standard 2-channel epon centerpieces (Beckman-Coulter). Measurements were performed at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... culture samples were diluted (1:100 or 1:1000) with 0.2-µm-filtered 18% BSW and were analysed with a Multisizer M4 Coulter cytometer (Beckman-Coulter) equipped with a 20 or 30 µm aperture tube ...
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bioRxiv - Biochemistry 2019Quote: ... followed by spinning at 158,000 g for 2 hours (Ti45 rotor, Beckman Coulter). The lysate was passed through a column containing 2 mL TALON superflow at 0.5 mL/min ...