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Citations for Beckman :
351 - 400 of 2271 citations for 5R 3 4 5 6 Tetrahydro 5 phenyl N benzyloxycarbonyl 4 H 1 4 oxazin 2 one since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Gradients were centrifuged at 36000 RPM for 2 hours at 4°C in a SW41 rotor (Beckman Coulter) and then fractions were collected using a gradient collector (Teledyne ISCO gradient station ...
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bioRxiv - Cell Biology 2020Quote: ... the cleared supernatant was ultracentrifuged at 47,000 g for 2 hours at 4 °C using the JA25.50 rotor (Beckman). The virus was resuspended overnight in 180 μL D10 medium at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... Digested extracts were then loaded on 1 M sucrose cushions 116 and centrifuged at 70,000 rpm for 2 hours at 4°C in a TLA100.4 rotor (Beckman). Ribosome pellets were resuspended in 300 µl of TRIzol reagent (Invitrogen) ...
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bioRxiv - Cancer Biology 2022Quote: ... 5×10^6 cells counted by Multisizer 4e Coulter Counter (Beckman) were plated on a 60 mm plate and serum starved before analysis unless otherwise indicated ...
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bioRxiv - Microbiology 2020Quote: ... RNA was pelleted by centrifugation for 24 h at 4 °C and 104.000 g in a SW28.1 swing out rotor (Beckman Coulter, Brea, CA, USA). RNA was taken up in 100 µl H2O and 500 µl TS buffer was added ...
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bioRxiv - Neuroscience 2022Quote: ... The crude viral lysate was purified with two rounds of CsCl gradient ultracentrifugation at 100,000×g and 4°C for 23 h using 13.5-mL Quick-Seal tubes and a NVT65 rotor (Beckman Coulter, Brea, CA). Each tube was placed gently and 500– 750 µL of white turbid bands were collected ...
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bioRxiv - Biochemistry 2020Quote: ... which was then centrifuged at 160,000×g at 4°C for 16 h in a Beckman MLS–50 rotor (Beckman–Coulter, CA, USA), and 24 fractions were collected ...
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Isolation and characterization of a novel phage of Vibrio parahaemolyticus with biocontrol potentialbioRxiv - Microbiology 2022Quote: ... for purification and centrifuged at 200,000 × g at 4°C for 24 h using an Optima L-100 XP ultracentrifuge (Beckman Coulter, CA, USA). The purified phage particles were dialyzed through 30-kDa superfilters (Millipore ...
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bioRxiv - Cell Biology 2022Quote: ... One milliliter of concentrated conditioned medium was pipetted on top of the gradient and samples were centrifuged for 18 h at 100,000 ×g and 4 °C using a SW 32.1 Ti rotor (Beckman Coulter, Fullerton, California, USA). Fractions of 1 mL were collected from the top and EV-rich fractions 9 and 10 (corresponding to a density of 1.10-1.12 g/mL ...
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bioRxiv - Molecular Biology 2024Quote: ... ADEV isolation was performed by ultracentrifugation at 100,000 × g for 14 h at 4 °C (swing bucket rotor on minimum acceleration/break setting, SW 32Ti Beckman Coulter, Brea, CA), and ADEVs were resuspended in 200μL PBS and stored at −20°C until used16,41 ...
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bioRxiv - Cell Biology 2023Quote: ... was next subjected to ultracentrifugation at 120,000 × g for 4 h in a SW 28 Ti Swinging-Bucket Rotor (Beckman Coulter, Fullerton, CA) to sediment small EVs ...
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bioRxiv - Biochemistry 2024Quote: ... Purified nucleosome arrays were subsequently isolated on 5-40% w/v sucrose gradients in 0.5X TE by centrifuging the gradients at 39,000 rpm and 4°C for 8 h in a SW41 Ti rotor (Beckman Coulter, Brea, CA). After centrifugation ...
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ACE2-lentiviral transduction enables mouse SARS-CoV-2 infection and mapping of receptor interactionsbioRxiv - Microbiology 2021Quote: ... UV-inactive SARS-CoV-2 was then partially-purified using a 20% sucrose cushion centrifuged at 175,000 x g for 3-4 hr using SW32Ti rotor (Beckman Coulter). The sample that has passed through the sucrose was collected and concentrated using Amicon Ultra-15 Centrifugal Filter Units with 100 kDa cutoff (Merck Millipore) ...
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bioRxiv - Molecular Biology 2023Quote: ... using 10-40 % sucrose gradients in buffer A after 3 hours of centrifugation at 35,000 rpm and 4 °C in SW41 rotor (Beckman Coulter). To isolate ribosomes for structural analysis ...
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bioRxiv - Molecular Biology 2022Quote: ... and spun at 40,000 rpm for 3 hours at 4°C using a SW 41 Ti Swinging-Bucket Rotor (Beckman Coulter). Sucrose gradient fractionation was performed by using a Brandel Density Gradient Fractionation System ...
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bioRxiv - Synthetic Biology 2023Quote: ... The gradients were ultra-centrifuged at 41,000 rpm for 3 hours at 4°C (Optima L-80 XP ultracentrifuge (Beckman-Coulter)) ...
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bioRxiv - Microbiology 2023Quote: ... Each tube was centrifuged for 3 hours at 150’000 x g (4°C) using an Optima XPN-80 ultracentrifuge (Beckman Coulter) equipped with a SW 32 Ti swinging bucket (369650 ...
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bioRxiv - Biochemistry 2023Quote: ... The sucrose density gradient was centrifuged at 50,000 rpm for 3 hours at 4°C in a TLS-55 swinging bucket rotor (Beckman Coulter) with minimum acceleration up to 5,000 RPM and deceleration with no break ...
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bioRxiv - Bioengineering 2023Quote: ... For performing cryogenic transmission electron microscopic imaging EVs were centrifuged and washed in PBS at 100,000 x g for 3 hours at 4°C in a SW41 Ti rotor (Beckman Coulter).
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bioRxiv - Biochemistry 2024Quote: ... Equivalent amounts of cell lysates prepared as described above were loaded onto the sucrose gradients and spun at 35,000 g for 3 hours at 4°C in a SW41 rotor (Beckman Coulter). After centrifugation the polysomes were fractionated and absorbance was monitored at 254 nm for RNA and 680 nm for GFP using the TriaxTM Flowcell (BioComp Instruments) ...
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bioRxiv - Biophysics 2021Quote: ... centrifuged (Beckman Avanti, 1500 x g, 10 min, 4 °C) and pellets were flash-frozen and stored at −80 °C.
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bioRxiv - Immunology 2022Quote: ... for 90 minutes at 4°C (Optima XE-90, Beckman-Coulter with SW 32 Ti swinging bucket rotor ...
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bioRxiv - Molecular Biology 2022Quote: ... and ultracentrifuged (60,000 rpm, 4 °C, Beckman, 70 Ti rotor) for 20 hours ...
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bioRxiv - Synthetic Biology 2020Quote: ... After clarification (20,000 rpm, Beckman JA 25.5, 30min 4°C), lysate was incubated with Glutathione S-sepharose 4B resin (GE Healthcare ...
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bioRxiv - Immunology 2020Quote: ... for 90 minutes at 4°C (Optima XE-90, Beckman-Coulter with SW 32 Ti swinging bucket rotor ...
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bioRxiv - Cell Biology 2022Quote: ... at 4°C in a TLA-55 rotor (Beckman Coulter) at maximum speed (186000 g ...
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bioRxiv - Bioengineering 2023Quote: ... the centrifugation at 12,000 rpm 4 ℃ (Beckman Avanti J-26) for 30 mins was performed to remove cell debris ...
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bioRxiv - Biochemistry 2023Quote: ... 4 °C (Fixed angle Ti90 Rotor, Optima XE90, Beckman Coulter). The supernatant was loaded onto a 70 ml Q-sepharose column (XK 26/20 ...
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bioRxiv - Biophysics 2024Quote: ... 4 °C for 45 min in an ultracentrifuge (Beckman Coulter). The top fraction containing liposomes and the attached motors was harvested after the flotation assay and motor concentration was measured by GFP fluorescence.
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Human Sex Matters: Y-linked lysine demethylase 5D drives accelerated male osteogenic differentiationbioRxiv - Cell Biology 2021Quote: ... Virus was transferred into conical tubes and spin at 20000rpm for 2 hours at 4°C in (Beckman TST2838) swinging bucket rotor ...
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bioRxiv - Biochemistry 2021Quote: ... 2 mM DTT) by being centrifuged at 73000 rpm for 45 minutes at 4°C (Beckman Coulter TL120.2 rotor). The mitoribosomal pellet was subsequently resuspended in ribosome resuspension buffer (25 mM HEPES-KOH pH = 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... Pellets were resuspended in 0.9% NaCl and spun at 110,000 × g for 2 hours at 4°C in an XPN-80 ultracentrifuge equipped with a Type 50.4 Ti rotor (Beckman Coulter ...
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bioRxiv - Molecular Biology 2023Quote: ... The gradients were centrifuged at 38,300 rpm for 2 hours and 45 min at 4 °C in a SW-40Ti (Beckman) rotor using an Optima XE-100 Ultracentrifuge (Beckman) ...
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bioRxiv - Molecular Biology 2023Quote: ... The gradients were centrifuged at 38,300 rpm for 2 hours and 45 min at 4 °C in a SW-40Ti (Beckman) rotor using an Optima XE-100 Ultracentrifuge (Beckman) ...
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bioRxiv - Plant Biology 2023Quote: ... Extract was then centrifuged at 40 000 rpm for 2 hours at 4°C using the 50Ti rotor (Beckman). A protocole previsouly used in the laboratory and described in Maclot et al ...
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bioRxiv - Molecular Biology 2023Quote: ... 1-1.5 μg of total protein was loaded onto a 10% to 50% sucrose gradient and centrifuged at 36,000 rpm for 2 hours at 4°C using an SW41 rotor (Beckman). Polysome profiles were visualized ...
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bioRxiv - Molecular Biology 2024Quote: ... were layered on top of each sucrose gradient and centrifuged (41,000 rpm for 2 hours at 4°C) in an SW41 Ti rotor (Beckman). UV profiling (254 nm ...
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bioRxiv - Microbiology 2021Quote: ... resuspended in 150 μl 0.1 M HEPES/NAOH pH 7.0 and purified for 48 h (250,000 × g, 4 °C; Beckman Optima LE-80K, rotor SW60-Ti) using a CsCl gradient (0.45 g/ml ...
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bioRxiv - Biophysics 2022Quote: ... EVs were fractionated by OptiPrep density gradient ultracentrifugation (100,000 × g, 18 h, 4 °C) using a SW41 rotor (Beckman Coulter, Fullerton, CA, USA) through a continuous 5–40% OptiPrep (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... the SBV samples were purified via sucrose density gradient centrifugation (10–50% range) at 32,500 rpm for 4 h with an SW 41 Ti Swinging-Bucket Rotor (Beckman Coulter Inc., Brea, CA). The SBV band that corresponded to the 40% sucrose gradient was harvested and then centrifuged at 25,000 rpm for 12 h ...
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bioRxiv - Biophysics 2023Quote: ... Virions were banded by density gradient centrifugation at 152,000 x g for 16 h at 4°C in a SW41 rotor (Beckman Coulter SRL, Milan, Italy). The gradient was then fractionated ...
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bioRxiv - Biochemistry 2024Quote: ... Nucleosome sucrose gradients were then centrifuged at 41,000 rpm and 4°C for 22 h in a SW41 Ti rotor in an Optima L-100 XP ultracentrifuge (Beckman Coulter, Brea, CA). After centrifugation ...
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bioRxiv - Biophysics 2021Quote: The aggregation products formed in Eppendorf tubes under agitation conditions at pH 3 and pH 4 were centrifuged using an Optima MAX-XP ultracentrifuge (Beckman Coulter) in a TLA-55 rotor at 40,000 rpm at 20°C for 45 min ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated for 1 hr at +4°C before the flow cytometry analysis (Beckman Coulter CytoFlex) or sorting (SONY MA900) ...
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bioRxiv - Biochemistry 2023Quote: ... The crude lysate was centrifuged for 1 hour at 20,000 rpm and 4 °C (Beckman Coulter). The supernatant was filtered using a 0.44 μm syringe filter (Sartorius ...
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bioRxiv - Biophysics 2023Quote: ... The cell lysate was centrifuged at 4 °C at 35000 rpm for 1 hour by Beckman Optima™ L-80XP ultracentrifuge equipped with a Ti-45 rotor (Beckman ...
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bioRxiv - Genomics 2020Quote: ... by ultracentrifugation at 4°C overnight (33500 rpm, Beckman, 70Ti rotor). The ribosome pellet was dissolved in 300 μL resuspension buffer (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Neuroscience 2020Quote: ... for 60 min at 4 °C on ultracentrifuge (Beckman L7-65) and rotor (Beckman SW41-Ti) ...
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bioRxiv - Cell Biology 2020Quote: ... 4°C (TLA-55 rotor, Optima MAX-XP benchtop ultracentrifuge, Beckman). The supernatant was carefully aspirated and discarded ...
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bioRxiv - Microbiology 2020Quote: ... and then analyzed with a Multisizer 4 Coulter cytometer (Beckman-Coulter) as described previously (6).