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Citations for Beckman :
151 - 200 of 2271 citations for 5R 3 4 5 6 Tetrahydro 5 phenyl N benzyloxycarbonyl 4 H 1 4 oxazin 2 one since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... 4°C (SW41 rotor, Beckman). The supernatant was concentrated with a 100-kDa molecular weight cutoff concentrator (Merck Millipore ...
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bioRxiv - Cell Biology 2024Quote: ... 4-laser Gallios (Beckman Coulter) flow cytometer ...
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bioRxiv - Microbiology 2020Quote: MT-2 cells were collected and washed three times by pelleting at 200 x g for 5 min at 4°C in an Allegra centrifuge (Beckman) and resuspending cells in ice-cold PBS ...
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bioRxiv - Cell Biology 2022Quote: ... brought up to 3 ml using sterile 20 mM Tris-HCl pH 7.5 and pelleted at 40,000g and 4°C for 60 minutes (TLA100.3 rotor, Optima Max-XP ultracentrifuge, Beckman Coulter, Inc.). Finally ...
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bioRxiv - Molecular Biology 2020Quote: ... The following day the viral pellet was resuspended in 5 ml PBS and centrifuged at 20,000 rpm for 90 mins at 4°C (Optima XL-100K, Beckman Coulter). The pellet was then resuspended in the required volume of PBS before being aliquoted and stored at −70°C.
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bioRxiv - Neuroscience 2022Quote: ... trypsin-dissociated cells on 96-well plates were centrifuged at 1,000g for 5 min at 4°C using a swinging-bucket rotor for microplates (Beckman S6096) in an Allegra X-30R centrifuge (Beckman) ...
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bioRxiv - Genomics 2023Quote: ... The suspension was incubated on ice for 5 min and centrifuged at 20,000 ×g (10 min, 4 °C, JA-20 rotor, Avanti J-26 XP, Beckman Coulter). The pellet was re-centrifuged for 5 min and both supernatants were combined into a new tube ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 mM DTT and centrifuged for 16 h at 34,000 rpm at 4°C for 16 h using an SW60 Ti rotor (Beckman Coulter). The fractions were subjected to SDS-PAGE and Coomassie blue staining.
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bioRxiv - Molecular Biology 2021Quote: ... 2 mM DTT and centrifuged for 16 h at 34,000 rpm at 4°C for 16 h using an SW60 Ti rotor (Beckman Coulter). The fractions ...
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bioRxiv - Pathology 2020Quote: ... Hemocytes stained with 4’-6-diamidino-2-phenylindole (DAPI) were analyzed on a CytoFLEX flow cytometer with CytExpert software (Beckman-Coulter, USA). Plots of side scatter SSC-A versus forward scatter FSC-A were used for visualization of cell groups ...
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bioRxiv - Cell Biology 2020Quote: ... Crude mitochondria were loaded on top of the chilled gradient and separated at 100,000 Å∼ g for 1 h at 4°C (SW41 rotor; Beckman). Intact purified mitochondria were recovered from a brown band at around 16% Histodenz™ concentration ...
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bioRxiv - Molecular Biology 2022Quote: ... The sucrose gradient was centrifuged at 24,400 rcf for 1 h at 4°C in a SW41Ti Swinging-Bucket Rotor (Beckman Coulter), and nuclei at the bottom of the gradient were resuspended in PBS ...
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bioRxiv - Plant Biology 2021Quote: ... The thylakoid membranes with different densities were analytically separated at 125,000 × g and 4°C for 1 h (SW 41 Ti, Beckman Coulter).
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bioRxiv - Neuroscience 2022Quote: ... 10 mM Tris-HCl) and centrifugated at 24,000 rpm for 1 h at 4°C in a SW41Ti Swinging-Bucket Rotor (Beckman Coulter). Supernatant was discarded and nuclei pellets were re-suspended in 800 µL of PBS ...
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bioRxiv - Plant Biology 2022Quote: ... in a 12.5 mL ultracentrifuge tube and centrifuged for 1 h at 4°C at 200,000 × g in a swinging bucket rotor (Sorval SW-41 Ti; Beckman Coulter), with moderate acceleration and deceleration ...
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bioRxiv - Molecular Biology 2024Quote: ... Lysates were cleared by centrifugation for 1 h at 45,000 rpm at 4°C (Optima XPN-80 Ultracentrifuge with 70 Ti, Beckman Coulter). HTP-tagged proteins were immunoprecipitated with 600 µl pre-washed IgG-sepharose 6 fast flow affinity resin (GE Healthcare ...
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bioRxiv - Neuroscience 2024Quote: ... Digested samples were ultracentrifuged at 100,000x g for 1 h at 4 °C using a TLA-55 rotor (Beckman Coulter) to concentrate the insoluble ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatant was ultracentrifuged at 80,000 × g for 1 h at 4°C using a type 50 Ti rotor ultracentrifuge (Beckman Coulter). The supernatant ...
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bioRxiv - Cancer Biology 2022Quote: ... Fractions 2–9 were diluted 1:10 in PBS and centrifuged at 20,000 ×g (bEVs) or 100,000 ×g (sEVs) at 4°C for 1 h with an MLS-55 rotor (Beckman Coulter). The bEV and sEV pellets were suspended in double 0.22-µm-filtered PBS and were either used directly for experiments or stored in aliquots at −80°C until subsequent BLI ...
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bioRxiv - Molecular Biology 2023Quote: ... and ultracentrifuged in a sucrose cushion at 100,000 rpm at 4°C for 1 h with a TLA110 rotor (Beckman Coulter). The ribosome pellet was dissolved in TRIzol reagent (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... loaded onto a discontinuous 0.75/1.0/2.33 M sucrose gradient and spin at 242,000 g for 1 h at 4 °C in a SW32Ti rotor (Beckman & Coutler). Growth cone fractions were collected at the interface load/0.75 M sucrose and non-growth cone fractions collected between 0.75/1.0 M sucrose and then analyzed by Western Blot.
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bioRxiv - Molecular Biology 2023Quote: ... Ribosomes were isolated by sucrose cushion ultracentrifugation at 100,000 rpm at 4°C for 1 h using TLA110 rotor (Beckman Coulter). RNAs in the ribosome pellet were isolated with TRIzol-LS (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... It was then centrifuged again at 100,000 x g for 1 h at 4 ⁰C (TLA 55 rotor, Beckman Coulter; OptimaTM Max ultracentrifuge, Beckman Coulter). The final pellet containing the purified EV fraction was resuspended in 50 µl of 20 mM Tris-HCl pH 6.0 ...
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bioRxiv - Plant Biology 2023Quote: ... at 10,000 x g for 1 h at 4 ⁰C (JA-17 rotor, Beckman Coulter; Avanti J-20TM centrifuge, Beckman Coulter). The supernatant from this step was decanted into the new tubes and centrifuged at 39,800 x g for 2 h at 4 ⁰C (JA-17 rotor ...
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bioRxiv - Plant Biology 2023Quote: ... The tubes were then centrifuged at 100,000 x g for 1 h at 4 ⁰C (TLA 55 rotor, Beckman Coulter; OptimaTM Max ultracentrifuge, Beckman Coulter). The pellets from the tubes were pooled together in 500 µl of 20 mM Tris-HCl pH 6.0 buffer and the volume was brought up to 1,500 µl using 20 mM Tris-HCl ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was then transferred to polycarbonate tubes and centrifuged at 100,000 × g and 4°C for 1 h in a XL-90 ultracentrifuge (Beckman Coulter) ...
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bioRxiv - Cell Biology 2022Quote: ... This spin was repeated and the resulting supernatant was then centrifuged for 1 h at 75,000 g at 4°C (TLA-100.3 rotor or Type60 Ti rotor; Beckman Coulter, USA). The supernatant was aspirated and the membrane pellet gently resuspended in microsome buffer (10 mM HEPES/KOH pH 7.5. ...
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bioRxiv - Molecular Biology 2023Quote: ... for 45[min at 25°C and then subjected to a sucrose cushion ultracentrifugation at 100,000[rpm for 1[h at 4°C with Optima MAX-TL ultracentrifuge and TLA-110 rotor (Beckman Coulter). The RNA fragments in the resulting pellet were recovered using TRIzol reagent (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... and centrifuged at 100,000 × g for 1 h at 4 °C using a TLA-55 rotor in an Optima TLX Ultracentrifuge (Beckman Coulter). The first supernatant ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were then centrifuged for 1 h at 100,000 g and 4°C in a TLS 55 rotor (Beckman-Coulter) with the appropriate adapters (#358614) ...
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bioRxiv - Microbiology 2024Quote: ... Ribosomes were isolated by sucrose cushion ultracentrifuged at 100,000 rpm (543,000 × g) for 1 h at 4°C with a TLA110 rotor and an Optima MAX-TL ultracentrifuge (Beckman Coulter). Subsequently ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by centrifugation at 36,000 rpm for 3 hours at 4°C (Beckman Coulter). RNA was then extracted from the monosomes fraction using Trizol (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... and extracellular vesicles including exosomes and virions were pelleted by ultracentrifugation at 141,000 g at 4 °C for 2 h in a swinging-bucket rotor (SW28; Beckman Coulter, Brea, CA, USA). The pellet was resuspended in filtered PBS and centrifuged at 18,300 g and 4°C for 10 min to remove insoluble aggregates ...
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bioRxiv - Microbiology 2020Quote: ... EMVs were obtained by ultracentrifugation from 4 mL of filtrate at 100,000 × g (average centrifugal force) and 4°C for 2 h with an OptimaX centrifuge (Beckman Coulter, Brea, CA, USA). The pellets were resuspended in 400 μL of Dulbecco’s phosphate-buffered saline (DPBS ...
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bioRxiv - Neuroscience 2022Quote: ... The tubes were sealed and centrifuged at 50,000 rpm at 4°C for 2 h in an OptimaTM L-90K ultracentrifuge using the 70.1Ti rotor (Beckman Coulter, Brea, CA, USA). After the centrifugation ...
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bioRxiv - Microbiology 2024Quote: ... 21000 rpm for 5 h (Beckman SW41Ti). The band of the expected density was pooled using a syringe and the buffer was exchanged to the E-buffer using 100kDa VivaspinTM columns (Cytiva) ...
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bioRxiv - Cell Biology 2022Quote: ... 4°C for 1 hour (JA 25.5 rotor, Beckman Coulter). The supernatant was kept and clarified by a 0.45 µM syringe filter (Pall Corporation) ...
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bioRxiv - Biophysics 2021Quote: ... and centrifuged at 200,000 × g for 1.5 h at 4°C in an SW41Ti rotor (Beckman). Fractions (1 ml each ...
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bioRxiv - Microbiology 2024Quote: ... Centrifugation was at 75,600 ×g for 22 h at 4°C using an SW41 rotor (Beckman). Gradients were fractionated by manual collection from the top and fractions containing VLPs were identified by western blot analysis using a blend of monoclonal antibodies for the detection of VP1 (MAB8566 ...
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bioRxiv - Biophysics 2021Quote: ... and the fractions containing the protein of interest were combined and concentrated to 2mL at a final concentration of 3.85 mg/mL by repeated centrifugation in an Amicon Ultra Centrifugal Filter at 4k rpm for 5 min at 4 °C (Beckman SX4250). The concentrated protein was incubated overnight with TEV (1:50 mass ratio ...
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bioRxiv - Biochemistry 2020Quote: ... cells were centrifuged at 4 ℃ for 5 minutes at 300g and suspended in HBSS and analyzed with a flow cytometer (CytoFLEX, Beckman Coulter). Mean fluorescence intensity was calculated for each group.
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bioRxiv - Cell Biology 2024Quote: ... We loaded 2 mL of the homogenate mixture on top of a 3.92 ml 62% sucrose in MAB cushion in an Ultra-Clear 5/8” X 4” Beckman Coulter tube (344061, Beckman Coulter). We added the remaining sucrose in MAB solutions ...
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bioRxiv - Cell Biology 2022Quote: ... A new supernatant was collected in a prechilled 1.5 ml ultracentrifuge tube and centrifuged at 100000 g for 1 h at 4°C in a TLA-55 rotor (Beckman Coulter). The resulting pellet was resuspended in the same isotonic buffer containing antiproteases and was frozen at -80°C together with the 100000 g supernatant ...
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bioRxiv - Microbiology 2020Quote: ... Vesicles were obtained by centrifugation at 38,400 x g for 1 h at 4°C in an Avanti J-20XP centrifuge (Beckman Coulter, Inc). Pelleted vesicles were resuspended in 20 ml of 50 mM HEPES (pH 6.8 ...
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bioRxiv - Microbiology 2024Quote: ... The final Hcp1-VLPs particles were pelletized by ultracentrifugation at 274,000 ×g at 4°C for 1 h in a SW41 Ti rotor (Beckman Coulter, Inc). The final pellet was resuspended in PBS pH 7.4.
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bioRxiv - Biochemistry 2024Quote: ... Non-solubilized materials were removed by centrifuging the sample at 41,000 r.p.m for 1 h at 4 °C using rotor type 45 Ti in the OptimaTM L-100 XP Ultracentrifuge (BECKMAN COULTER). The resulting supernatant containing the solubilized LysP-GFP was incubated with Ni2+-NTA resin (Qiagen ...
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Sense codon-misassociated eRF1 elicits widespread ribosome stalling and induction of quality controlbioRxiv - Molecular Biology 2024Quote: ... The mixture was layered over 900 μl of 1 M sucrose cushion 46 and ultracentrifuged at 100,000 rpm ( 543,000 × g) at 4°C for 1 h using a TLA-110 rotor and an Optima MAX-TL Ultracentrifuge (both Beckman Coulter).
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bioRxiv - Microbiology 2024Quote: ... the supernatant was filtered through 0.45-μm syringe filters and ultracentrifuged at 100,000 ×g for 1 h at 4 °C (MLA-50 fixed angle rotor, Beckman Coulter, Germany). The supernatant was discarded ...
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bioRxiv - Cancer Biology 2021Quote: ... 4°C (Beckman Coulter Allegra X15r). After the final wash to remove Matrigel ...
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bioRxiv - Molecular Biology 2021Quote: ... 4°C (SW41Ti rotor, Beckman Coulter) and 16 fractions were collected ...