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Citations for Beckman :
351 - 400 of 736 citations for 3 hydroxy 20 oxopregn 5 en 21 yl acetate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... MB concentration and number- and volume-weighted size distributions were measured using a Multisizer 3 Coulter Counter (Beckman Coulter). MB concentration (đi ...
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bioRxiv - Microbiology 2024Quote: ... the supernatants were loaded onto 1 mL of 30% sucrose in PBS buffer and centrifuged at 25,000 rpm at 4°C for 3 hours using the 90AT rotor (Beckman). The pelleted viral particles were resuspended in 100 ΌL of PBS and fixed with formaldehyde (working concentration 0.1% ...
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bioRxiv - Neuroscience 2024Quote: ... Tubes were centrifugated at 100,000 x g (32,500 RPM) for 3 hours at 4°C using a SW55Ti rotor (Beckman Coulter). Synaptosomes enriched in the interface between 1.0-1.25 M sucrose were collected and diluted with ice-cold 0.1 mM CaCl2 ...
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Syntaxin-2 inhibits excessive phagocytic uptake but promotes phagolysosomal clearance in macrophagesbioRxiv - Cell Biology 2024Quote: ... followed by sequential topping with 2 mL each of 35, 25, and 10% sucrose (all prepared in 3 mM imidazole, pH 7.4) in SW40 tubes (344060, Beckman). The samples were then centrifuged at 100,000 g for 1 hr at 4°C using a SW40Ti rotor (Beckman) ...
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bioRxiv - Bioengineering 2024Quote: ... the distribution of microbubble diameter and concentration was assessed using a Coulter counter (Multisizer 3; Beckman Coulter, Fullerton, California). T1 mapping MRI sequences were used to guided sonication targeting ...
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bioRxiv - Cell Biology 2024Quote: ... The 100,000xg SEV-containing pellets were resuspended in 3 ml sterile cold PBS and repelleted at 100,000xg for 4 hrs in a TLA110 rotor (Beckman). SEVs were analyzed for size and number by nanoparticle tracking (ZetaView ...
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bioRxiv - Molecular Biology 2021Quote: ... Sucrose density gradients (5%â45% w/v) were freshly made in SW41 ultracentrifuge tubes (Beckman) using a Gradient Master (BioComp Instruments ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 mM MgCl2) and centrifuged at 45,000 rpm (SW 55 Ti rotor, Beckman Coulter, Inc.) (adapted from Liang et al ...
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bioRxiv - Microbiology 2021Quote: ... Product were PCR amplified by 5 cycles and cleaned up using SPRI beads (Beckman Coulter) and subjected to paired-end 300bp sequencing on Illumina Miseq ...
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bioRxiv - Biochemistry 2023Quote: ... Ultracentrifugation was carried out for 5 hours at 133,000xg on a SW60-Ti (Beckman Coulter) rotor to resolve each gradient ...
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bioRxiv - Cancer Biology 2023Quote: ... After 5-7 days cells were counted using a Z1 Coulter Particle Counter (Beckman Coulter).
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bioRxiv - Biophysics 2024Quote: ... the microtubules were centrifuged for 5 min at 22,000 RPM (TLA100.0, Beckman Optima MAX-XP) at 37°C ...
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bioRxiv - Genomics 2024Quote: ... After 5 days cells were harvested for genomic DNA purification using Agencourt DNAdvance (Beckman Coulter). Transfection of human primary hepatocytes were performed using Lipofectamine MessengerMAX Transfection Reagent (Thermo Fisher) ...
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bioRxiv - Microbiology 2023Quote: ... pH 7.4) and loaded into a centrifuge tube (Beckman Cat. No. 344057, 5 ml capacity). One milliliter of 20% sucrose in 20 mM HEPES pH 7.4 ...
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bioRxiv - Immunology 2024Quote: ... and BA.5 RBD were sorted using the MoFlo Astrios EQ Cell Sorter (Beckman Coulter). The data from FACS were gathered using Summit 6.0 and analyzed with FlowJo software version 10.8.
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bioRxiv - Biophysics 2020Quote: ... Escherichia coli BL21 cells overexpressing α-synuclein were collected by centrifugation (20 min, 4000 rpm, 4 °C) in a JLA-8.1000 rotor in a Beckman Avanti J25 centrifuge (Beckman Coulter), resuspended in lysis buffer (10 mM Tris ...
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bioRxiv - Genomics 2022Quote: ... For plasma samples that were found to have significant genomic DNA contamination (fragment size > 240 base pairs for more than 20% of fragments at library preparation) we performed a 0.4x cleanup using SPRIselect magnetic beads (Beckman Coulter) on the extracted cfDNA.
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bioRxiv - Biochemistry 2020Quote: ... The homogenate was then sequentially centrifuged at 10,000xg for 20 min in an Avanti JE centrifuge (Beckman, Palo Alto, CA) and at 100,000xg for 45 min on a Beckman L8M centrifuge at 10 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... Tubes were then spun at 10,000 x g for 20 minutes at 4 °C in an ultracentrifuge (Beckman, L8-70M). The supernatant was filtered using 0.2 ΌM syringe filters (Millipore Sigma ...
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bioRxiv - Cell Biology 2021Quote: Microtubules were sedimented onto poly-L-lysine coated coverslips by centrifuging through 20% glycerol BRB80 cushion at 4700 rpm for 2 h in S6096 rotor (Beckman). Coverslips were subsequently fixed with 20°C methanol ...
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bioRxiv - Plant Biology 2021Quote: ... The plant debris was removed by centrifuging at 16 000 g for 15 minutes at 4°C in a JA-25.50 rotor and Avanti J- 20 XP centrifuge (Beckman Coulter). The clear supernatant was then gently poured on top of 8 ml sucrose cushion (100 mM Tris·HCl (pH 8.0) ...
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bioRxiv - Microbiology 2021Quote: ... The density gradient centrifugation was performed at 141,000 g and 4 °C for 20 h in a SW28 swing rotor (Beckman Coulter). A total of 18 fractions were collected starting from the top.
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bioRxiv - Microbiology 2021Quote: ... the wafer clump was collected using a pipette tip and resuspended in 3 mL of 2x Buffer C and mixed with 3 mL of 1x Buffer C (20 mM MES-Tris, 10 mM MgCl2, 50 mM KCl, pH 6.9) and pelleted in the ultracentrifuge (Beckman 50Ti). The resulting pellet was then resuspended in 200 ”L of 1x Buffer C with 10% glycerol ...
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bioRxiv - Microbiology 2021Quote: ... âExâ mediumâ was prepared by sedimenting 35 ml of âRPMI+â medium supplemented with 20 % FBS by ultracentrifugation in a SW32 or SW28 swing-out rotor (Beckman) at 4 °C for at least 16 h ...
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bioRxiv - Microbiology 2020Quote: ... lysate was collected and centrifuged at 3000x rpm for 20 minutes to pellet cell debris (Beckman Coulter Allegra X-14R). This procedure was repeated for a second passage working stock with collection at 48hpi and titered by TCID50 assay.
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bioRxiv - Cell Biology 2020Quote: 5-20% sucrose gradients were prepared on a Biocomp Gradient Station IP from 5% and 20% solutions of sucrose in gradient buffer (50mM Tris HCl pH7.5, 150mM NaCl, 1mM EDTA) to Seton tubes (Beckman Coulter). Single cell suspensions of approximately 3Ă108 cells from thymus and bone marrow were obtained as above ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were incubated in dark for 20 min followed by fluorescence analysis by Gallios Flow Cytometer 561 ready (Beckman Coulter). UV (355 nm ...
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bioRxiv - Microbiology 2022Quote: ... The capsid-host protein complexes were sedimented through a 30% sucrose cushion at 110,000 g for 20 min at 4°C (TLA-100, Beckman-Coulter), resuspended in CBB by ultrasound tip sonication at 40 W for about 5 x 5 seconds on ice ...
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bioRxiv - Biochemistry 2022Quote: Sedimentation velocity analytical ultracentrifugation was performed at 42,000 rpm at 20° C in an An-60 Ti rotor (Beckman Coulter), using standard charcoal double-sector centrepieces ...
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bioRxiv - Microbiology 2021Quote: ... Samples were then diluted 20 folds and measured at 595 nm using a UV/Vis Spectrophotometer (Beckman Coulter DU-800). The biofilm formation was quantified as A595/cm2 ...
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bioRxiv - Bioengineering 2020Quote: ... cDNA from each sample was generated with Smart-seq2 protocol using 20 ”L total reaction volume and roughly 200 ng RNA input followed by 0.8X AMPure XP beads (Beckman Coulter) purification ...
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bioRxiv - Cancer Biology 2020Quote: ... samples were suspended in FACS buffer (PBS, 20 mM EDTA pH 8.0, 2% FCS) and acquired using a CyAn ADP9 flow cytometer (Beckman Coulter), FACS LSRII Fortessa or FACSymphony (both BD Biosciences) ...
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bioRxiv - Biochemistry 2020Quote: ... by dounce homogenization (20 strokes) and the homogenate was centrifuged at 55,000 rpm in a Type 70 Ti rotor (Beckman Coulter) for 1 hr ...
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bioRxiv - Microbiology 2020Quote: VLPs were collected after removal of cells from the enrichment culture (7,000 rpm, 20 min, Sorvall 1500 rotor) and concentrated by ultracentrifugation (40,000 rpm, 2h, 10°C, Beckman SW41 rotor). The particles were resuspended in buffer A ...
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bioRxiv - Microbiology 2021Quote: ... The treated cells were pelleted by centrifugation at 12,000Ăg at 4 °C for 20 min (Beckman Allegra X-15R) and the supernatant was discarded ...
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bioRxiv - Cancer Biology 2021Quote: ... genomic DNA was purified by mixing 20 ÎŒl of the lysed samples with 50 ÎŒl Ampure XP beads (Beckman Coulter), and left for 5 minutes at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... and cellular debris was removed by centrifugation at 90 000 x g for 20 min at 4°C (45 Ti rotor, Beckman). Membranes were collected by ultracentrifugation at 220 000 x g for 90 min at 4°C (45Ti rotor ...
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bioRxiv - Microbiology 2020Quote: ... Microbial cells were collected from to 2-3 L of water samples by centrifugation at 12,000 à g and 4°C for 20 min in a high-speed centrifuge (Beckman, USA). Total genomic DNA was extracted using AxyPrep⹠Genomic DNA Miniprep Kit (Axygen ...
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bioRxiv - Immunology 2020Quote: ... PE-CD11b and FITC-CD3 (all Ebioscience) for 20 minutes and washed before sorting using a Moflo Astrios (Beckman Coulter) or FACSAria IIu (BD BioSciences) ...
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bioRxiv - Biophysics 2023Quote: ... Reactions were centrifuged at 400,000 g at 20 °C for 15 min in polycarbonate centrifuge tubes (Beckman Coulter Inc. USA). The pellets were resuspended in 40 Όl reaction buffer ...
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bioRxiv - Genomics 2023Quote: ... the supernatant was cleared by two rounds of centrifugation at 20,000 Ăg (10 min, 4 °C, JA-20 rotor, Avanti J-26 XP, Beckman Coulter) followed by centrifugation at 31,900 Ăg (10 min ...
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bioRxiv - Genomics 2023Quote: ... Precipitated ribosomes were pelleted at 20,000 Ăg (10 min, 4 °C, JA-20 rotor, Avanti J-26 XP, Beckman Coulter). To remove residual supernatant the pellet was re-centrifuged for 5 min at 20,000 Ăg ...
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bioRxiv - Plant Biology 2023Quote: ... The cAW was collected by spinning at 700 x g for 20 min at 4 â°C (JA-14 rotor, Beckman Coulter ...
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bioRxiv - Neuroscience 2023Quote: ... Homogenized tissue was sonicated 20 times with 1 sec pulses and then centrifuged using a SW55 Ti rotor (Beckman Coulter) at 42,700 rpm for 15 min at 4°C ...
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bioRxiv - Synthetic Biology 2022Quote: ... Cells were lysed using a sonicator to deliver ~ 8000 J of energy and lysate was clarified at 18000 rpm in a JA-20 rotor (Beckman) for 30 min at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... and purified and concentrated by ultracentrifugation on a 20% sucrose cushion (24,000 rpm and 4°C for 2 hours with a SW32Ti or SW28 rotor (Beckman Coulter)) ...
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bioRxiv - Biochemistry 2023Quote: ... by dounce homogenization (20 strokes).The lysate was clarified by centrifugation at 56,000 rpm in a Type 70 Ti rotor (Beckman Coulter) at 4°C for 1 hour ...
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bioRxiv - Cell Biology 2023Quote: ... and pelleted at 100,000xg for 20 min at 4 °C using fixed angle rotor (TLA-100 rotor, Beckman Coulter, 349481) in a benchtop ultracentrifuge (OptimaTL ...
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bioRxiv - Biochemistry 2022Quote: ... A soluble fraction was obtained after centrifugation of the lysate at 19,000 rpm for 40 min at 4 °C performed using Avanti J-E with a rotor JA-20 (BECKMAN COULTER) to remove insoluble debris.
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bioRxiv - Cancer Biology 2022Quote: ... step gradients were made by superposing sucrose solutions of different concentration (50%, 40%, 30%, 20%, 10%) in an ultra-clear polycarbonate tube (Beckman), and a linear gradient was allowed to form overnight at 4 °C ...