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Citations for Beckman :
501 - 550 of 736 citations for 3 hydroxy 20 oxopregn 5 en 21 yl acetate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... with densities ranging from 1.10 to 1.15 were pooled and washed in PBS (Beckman Ti50, 3 h at 42 000 rpm).
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bioRxiv - Microbiology 2022Quote: A 3 ml volume of pseudoparticles was pelleted using a TLA-55 rotor with an Optima-MAX-E ultracentrifuge (Beckman Coulter) for 2 hours at 42,000 rpm at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 nM leptomycin b, vehicle control or library compounds (Supplementary Tables 2, 3) were added using an Echo liquid handler (Labcyte/Beckman Coulter). Cells were incubated for a further 24 h then fixed in 4% paraformaldehyde and stained with DAPI and Alexa Fluor 488 Phalloidin (ThermoFisher) ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were then enriched in DNA sizes between 200 and 600 bp (corresponding to 1 to 3 nucleosomes) using AMPure XP magnetic beads (Beckman-Coulter), following the manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2020Quote: ... 3 ml of 50% and 3 ml of 40% sucrose) in Ultra-Clear Beckman tubes and centrifuged at 26000 rpm (SW32Ti rotor, Beckman Coulter) for 1 h at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... and pelleted for 3 min in a swing-out rotor at 1000 rpm in a tabletop centrifuge (Allegra X-30R, Beckman Coulter). The supernatant was aspirated and cells were suspended in the remaining liquid to 1 × 105 – 1 × 107 cells/100 μl ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was ultracentrifuged at 120,000 g for 3 h at 4°C using an MLS-50 rotor (Beckman Coulter, USA) to collect 120k pellet vesicles ...
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bioRxiv - Genetics 2020Quote: ... Timepoints were measured every 2-3 days by flow cytometry analysis of >7,000 cells (either a BD Accuri C6 or Beckman Coulter CytoFLEX). Data was analyzed using Cytoflow (https://github.com/bpteague/cytoflow ...
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bioRxiv - Biophysics 2021Quote: The aggregation products formed in Eppendorf tubes under agitation conditions at pH 3 and pH 4 were centrifuged using an Optima MAX-XP ultracentrifuge (Beckman Coulter) in a TLA-55 rotor at 40,000 rpm at 20°C for 45 min ...
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bioRxiv - Genomics 2020Quote: ... We carried out the first round of PCR for 3 cycles and purified the reactions with Ampure XP beads (Beckman-Coulter). We then carried out a second round of PCR with primers targeting the P5 and P7 sequences only ...
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bioRxiv - Genomics 2021Quote: ... samples were purified with 45 µl of Agencourt AMPure XP magnetic beads with a sample to beads ratio of 3:2 (Beckman Coulter), normalized by Qubit quantification and pooled to generate a 4 nM library ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were harvested after 3 days and counted using a Z2 Coulter Particle Count and Size Analyzer (Beckman Coulter, Brea, CA).
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bioRxiv - Cancer Biology 2024Quote: ... The percentage of fluorescence-positive cells was determined initially (baseline reference, passage 0, 3 days after transduction) and all following passages using the CytoFLEX Flow Cytometer (Beckman Coulter). In each passage ...
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bioRxiv - Cell Biology 2023Quote: ... 30% Percoll (v/v)] followed by 3 mL of MRB in a 13 mL ultracentrifuge tube (Beckman Instruments, Inc, CA 331372) and spun at 95,000 x g for 45 min in a TH641 rotor ...
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bioRxiv - Genetics 2023Quote: ... the cell pellet was resuspended in PBS supplemented with 2 % FCS and nucleated cells were counted in methylene blue with 3 % acetic acid on a Vi-Cell XR cell viability counter (Beckman Coulter). 10 × 106 bone marrow cells were resuspended in 200 μl of PBS supplemented with 2 % FCS containing the following antibody solution ...
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bioRxiv - Bioengineering 2022Quote: ... Supernatant (33 mL) was placed on a 3 mL 25% (w/v) sucrose pad in SW28 tube (Beckman, Brea, CA, USA) and centrifuged at 24,000 rpm ...
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bioRxiv - Cancer Biology 2023Quote: Counting of CB CD34+ cells after 3 days of ex vivo co-culture was accurately obtained with flow-count fluorosphere beads (Beckman Coulter), in combination with the following antibodies ...
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bioRxiv - Microbiology 2024Quote: ... and resolved by ultracentrifugation at 36,000 rpm for 3 h at 4°C with the fastest braking in Optima XPN-80 Ultracentrifuge equipped with SW-41Ti rotor (Beckman Coulter). Gradients were fractionated (0.5 mL/fraction ...
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bioRxiv - Immunology 2024Quote: ... Fluorescence signals and TruCount beads were acquired on a 3-laser CytoFLEX using Cytexpert software 2.3 (Beckman Coulter, Brea, CA, USA). Instrument quality control and standardization were performed daily using CytoFLEX Daily QC Fluorospheres (Beckman Coulter #B53230).
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bioRxiv - Cancer Biology 2024Quote: ... washed 3 times with chilled phosphate-buffered saline (PBS) and analyzed by flow cytometry (CytoFLex, Beckman Coulter Inc., Brea, CA, USA)[39 ...
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bioRxiv - Molecular Biology 2020Quote: ... The resulting cDNA was PCR-amplified to about 10–20 ng/μl using a high-fidelity DNA polymerase and purified with the Agencourt AMPure XP Kit (Beckman Coulter Genomics). The cDNA samples were pooled based on the ratios according to the RNA concentrations of the input samples ...
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bioRxiv - Biophysics 2021Quote: ... then the supernatant was ultracentrifuged at 100000×g for 20 min at 4 °C using an Optima L-100K ultracentrifuge (Beckman Coulter, USA) equipped with a SW 40 Ti swinging bucket rotor ...
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bioRxiv - Biochemistry 2020Quote: ... Triton-X was added to the lysate to a final concentration of 0.1% and clarified by spinning at 15,000 g for 45 minutes (Beckman Coulter JA-20 rotor). The supernatant was loaded onto a 5 mL HisTrap™ FF crude column (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was warmed to 37°C for few minutes until it turned cloudy following which it was centrifuged at 11,000 rpm at room temperature for 10 minutes (Beckman JA-20 rotor) to separate the soluble and detergent-enriched phases ...
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bioRxiv - Biophysics 2021Quote: ... and grown overnight at 37°C for 4 h and harvested by centrifugation in a Beckman Avanti J25 centrifuge with a JA-20 rotor at 5,000 rpm (Beckman Coulter, Fullerton, CA). The cell pellet was resuspended in 10 mM Tris ...
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bioRxiv - Microbiology 2020Quote: ... were pelleted through a 20% (wt/vol) sucrose cushion at 25,000 rpm for 2 h in an SW41 rotor (Beckman Coulter, Tokyo, Japan). Then ...
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bioRxiv - Microbiology 2022Quote: ... virions collected over the three passages were concentrated on 20% sucrose cushions (prepared in 1X phosphate buffered saline (PBS)) by ultracentrifugation (28000 rpm, 4°C, 90min, Beckman SW41 rotor). Genomic RNA was isolated from pelleted virions using Trizol per manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... 50 μl of each sample was centrifuged at a speed of 180000Xg (180000 RCF) in 7 mm x 20 mm polycarbonate centrifugation tubes (Beckman Coulter, USA) for at least 2h ...
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bioRxiv - Microbiology 2021Quote: ... unbound compound was separated from the astodrimer:virus mixture by pelleting the preincubated mixture through a 20% sucrose cushion (Beckman SW40 Ti rotor). The astodrimer sodium-containing supernatant was removed (i.e. ...
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bioRxiv - Biochemistry 2022Quote: ... Triton-X was added to the lysate at a final concentration of 0.1% and then clarified by spinning at 14,000 rpm for 45 min (Beckman Coulter JA-20 rotor). The supernatant was loaded onto a 5 ml crude Ni-NTA column (GE healthcare ...
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bioRxiv - Microbiology 2020Quote: ... the Exos were washed three times with PBS before ultracentrifugation at 100,000□x□g for 20□min at 4□°C using Beckman L7-80 and rotor SW41 (Beckman Coulter, Indianapolis, IN) to remove unbound stain ...
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bioRxiv - Genomics 2020Quote: ... Samples were eluted in 20 μL of elution buffer and 10 μL of each sample was pooled and concentrated to 20 μL using 0.7x AMPureXP beads (Beckman Coulter, Brea, CA). The final pooled sample was quantified using a Qubit Fluorometer and High Sensitivity DNA assay (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... Triton-X was added to the lysate to a final concentration of 0.1% and clarified by spinning at 15,000 RCF for 45 minutes (Beckman Coulter JA-20 rotor). The supernatant was loaded onto a 5 mL HisTrap™ FF crude column (GE Healthcare ...
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bioRxiv - Neuroscience 2020Quote: ... The cells were harvested by centrifugation at 4,000 g for 20 minutes in an Avanti J-26 XPI centrifuge with a JLA 8.1000 rotor (Beckman Coulter, Brea, CA, USA).
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bioRxiv - Evolutionary Biology 2020Quote: ... Mitochondria were purified using a series of differential centrifugations: 20 min at 1500 g to pellet residual chloroplasts (8 °C, 3500 RPM, Beckman JA-12), 20 min at 20 000 g to pellet mitochondria (8 °C ...
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bioRxiv - Microbiology 2020Quote: ... Particles from media were concentrated by ultracentrifugation through a 20% (wt/wt) sucrose cushion (90 min at 27,000 rpm in a Beckman SW32 rotor; Beckman Coulter Life Sciences). Pelleted particles were resuspended in PBS and stored in aliquots at −80°C.
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bioRxiv - Microbiology 2020Quote: ... followed by concentration of fixation-inactivated virions from media by ultracentrifugation through a 20% (wt/wt) sucrose cushion (120 min at 27,000 rpm in a Beckman SW32 rotor; Beckman Coulter Life Sciences). Pelleted particles were resuspended in PBS and stored in aliquots at −80°C.
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bioRxiv - Immunology 2020Quote: ... Data were acquired with a LSR Fortessa™ X-20 Flow cytometer (Becton Dickinson) and results analyzed with Kaluza software (Beckman Coulter).
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bioRxiv - Synthetic Biology 2023Quote: ... The PCR products were analyzed with 2% agarose gel and transferred into 96-well PCR plate wells for clean-up with 20 µL of AMPure XP beads (Beckman Coulter #A63881) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... The lysate was clarified by centrifugation at 5500 rpm for 20 min at 4°C (AllegraTM 21R Centrifuge, Beckman Coulter, CA, USA). The sample was then loaded into a HisTrap HP 5 mL column (Cytiva ...
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bioRxiv - Microbiology 2023Quote: ... Cells were incubated further overnight at 20°C and subsequently harvested by centrifugation (Beckman rotor 8.1000, 5000 rpm, 15 min, 4°C). The pellet was resuspended in PBS ...
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HIV-2 Immature Particle Morphology Provides Insights into Gag Lattice Stability and Virus MaturationbioRxiv - Microbiology 2023Quote: ... The pellet was resuspended in STE buffer and banded out over an OptiPrepTM step gradient (10, 20, 30% OptiPrepTM (v/v)) with a SW55 Ti rotor (Beckman Coulter, CA) at 45,000 rpm for 3 h ...
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bioRxiv - Microbiology 2023Quote: ... concentration of fixation-inactivated virions from media were spun down by ultracentrifugation through a 20% (wt/wt) sucrose cushion (120 min at 27,000 rpm in a Beckman SW32Ti rotor; Beckman Coulter Life Sciences). Pelleted particles were resuspended in PBS and stored in aliquots at −80°C.
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bioRxiv - Immunology 2023Quote: ... Data were acquired on a LSR Fortessa™ X-20 Flow cytometer (Becton Dickinson) and the results analyzed using Kaluza software (Beckman Coulter).
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bioRxiv - Biophysics 2023Quote: ... The virions were concentrated by ultracentrifugation through a 20% (w/v) sucrose cushion (2 h at 28,000 rpm in a SW32 rotor [Beckman Coulter Life Sciences]). The pellet was resuspended in PBS ...
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bioRxiv - Immunology 2022Quote: ... Data were acquired with a LSR Fortessa™ X-20 Flow cytometer (Becton Dickinson) and results analyzed with Kaluza software (Beckman Coulter).
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was warmed to 37°C for few minutes until it turned cloudy following which it was centrifuged at 11,000 rpm at room temperature for 10 minutes (Beckman JA-20 rotor) to separate the soluble and detergent-enriched phases ...
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bioRxiv - Biochemistry 2022Quote: ... Triton-X was added to the lysate to a final concentration of 0.1% and clarified by spinning at 15,000 RCF at 4°C for 45 minutes (Beckman Coulter JA-20 rotor). The supernatant was loaded onto a 5 mL HisTrap™ FF crude column (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... VLPs were concentrated over 500 μL of 20% sucrose in PBS at 50,000 x g for 45 min (Beckman Coulter Optima TLX). Infections were assessed by western blot using the following antibodies ...
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bioRxiv - Immunology 2023Quote: ... Cells were examined by flow cytometry using a BD LSR Fortessa™ X-20 and data were analysed with the Kaluza software (Beckman Coulter). Dead cells were excluded from the analysis and the strategy described by Roos et al ...