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Citations for Beckman :
451 - 500 of 736 citations for 3 hydroxy 20 oxopregn 5 en 21 yl acetate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... microtubules were spun down at 200,000 × g for 5 min using an Airfuge ultracentrifuge (Beckman-Coulter) to exclude free GTP ...
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bioRxiv - Immunology 2022Quote: ... and centrifuged (400×g for 5 min at 4°C) using a swinging bucket rotor (Beckman Coulter Avanti J-15RIVD with JS4.750 swinging bucket ...
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bioRxiv - Cancer Biology 2024Quote: ... and centrifuged at 100,000 × g for 5 hours at 4°C (MLS-50, Beckman Coulter, 326819). Afterwards ...
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bioRxiv - Cell Biology 2021Quote: ... then 2ml of 40% sucrose (in buffer C) and 1ml of 20% sucrose (in buffer C) were sequentially overlaid and the SW55 tubes (13×51 mm, Beckman Coulter) were centrifuged at ∼150,000×g (38,500 RPM ...
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bioRxiv - Genetics 2020Quote: ... The homogenates were incubated on ice for 15–20 min and then centrifuged at 3000xg for 10 min at 4°C in a GPR Data Settop centrifuge (Beckman, Inc.). The supernatant solutions were centrifuged at 15,800xg for 15 min at 4°C in a microcentrifuge (Model 5042 ...
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bioRxiv - Neuroscience 2020Quote: ... Each pool of samples was then purified using 79 μL beads buffer (20% PEG-8000 in 2.5 M NaCL, final concentrations) and 50 μL Ampure XP Beads (Beckman Coulter, A63881), and eluted in 7 μL NF H2O.
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bioRxiv - Biochemistry 2020Quote: ... to remove intact cells and the supernatant was centrifuged again at 25,000 g for 1 hour (Beckman Coulter JA- 20 rotor). The pellet was resuspended in lysis buffer and sodium cholate was added to a final concentration of 1% and stirred at 4°C for 1 hour ...
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bioRxiv - Cell Biology 2021Quote: ... The sample was overlaid with decreasing concentrations of Nycodenz (30%, 20%, 10% and 0%) and centrifuged in a SW55 rotor (Beckman Coulter) 2h 30 min at 55000 rpm and 4°C ...
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bioRxiv - Microbiology 2020Quote: ... The resulting cDNA was PCR-amplified to about 10-20 ng/μl using a high-fidelity DNA polymerase followed by purification using the Agencourt AMPure XP kit (Beckman Coulter). The cDNA samples were pooled with ratios according to the RNA concentrations of the input samples and a size range of 200-550 bp was eluted from a preparative agarose gel ...
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bioRxiv - Plant Biology 2022Quote: ... Thylakoid membranes were extracted via ultracentrifugation at 24,000 rpm for 1 h and 20 min (Beckman Coulter SW 32 Ti rotor). Thylakoids were collected from the step gradient interphases with a Pasteur pipet ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were incubated for 2h at 20°C in an AN60-Ti rotor in the Optima-AUC analytical ultracentrifuge (Beckman Coulter) before data acquisition for 15h at 42,000 rpm ...
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bioRxiv - Microbiology 2020Quote: ... the virus was purified and concentrated on a 20% sucrose cushion in phosphate buffered saline (PBS) in Ultra Clear Centrifuge tubes (Beckman Coulter), via ultracentrifugation at 4°C at 31,000 x g in a SW32Ti swinging bucket rotor (Beckman Coulter) ...
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bioRxiv - Microbiology 2020Quote: Larger amounts of MV-ZIKV and control MV supernatants were spun through a 20% sucrose cushion in an SW32 Ti rotor (Beckman, Inc.) at 25,000 rpm for 2 hours ...
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bioRxiv - Neuroscience 2022Quote: ... dispersed and collected in a single tube and further centrifuged for 4 hours at 21000 rpm in a 20% sucrose gradient (SW55 Beckman rotor). The resulting pellet was suspended in 100 μl PBS and the virus was aliquoted (5-10 μl ...
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bioRxiv - Cell Biology 2020Quote: ... Reconstituted nucleosome samples were overlaid onto the sucrose gradient and spun at 32000 rpm for 20 h at 4 ° using SW55Ti rotor (Beckman Coulter) in Beckman Optima L80 ...
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bioRxiv - Cell Biology 2020Quote: FCS-EV depleted medium was first prepared by centrifuging DMEM with 20% FCS at 200Kg overnight with a 45Ti rotor (Beckman Coulter). Supernatant was then recovered by pipetting without taking the last 5mL to avoid disturbing the pellet ...
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bioRxiv - Biochemistry 2021Quote: ... to remove intact cells and the supernatant was centrifuged again at 25,000 RCF for 1 hour (Beckman Coulter JA-20 rotor). The pellet was resuspended in lysis buffer and sodium cholate was added to a final concentration of 1% and stirred at 4°C for 1 hour ...
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bioRxiv - Developmental Biology 2021Quote: ... The samples were then loaded onto 20%-50% sucrose gradients and ultracentrifuged in a SW41 Ti swinging-bucket rotor (Beckman 331362) at 38,000 rpm for 2.5 h at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... Pellets were solubilised in 150 μL of cold TNE buffer and a volume of 1.5 ml was laid on a five step 20 - 60% (w/v) sucrose gradient prepared in Ultra-Clear 13 ml tubes (Beckman Coulter). The tubes were centrifuged at 35,000 rpm for 2h at 4°C in a Beckman SW41 rotor ...
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bioRxiv - Microbiology 2020Quote: ... The filtrate was subsequently centrifuged at 4,000 rpm for 20 minutes using an Avanti J-26 XPI centrifuge (Beckman Coulter, Inc.) to concentrate the bacterial consortium and remove residual organic carbon from the media ...
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bioRxiv - Microbiology 2020Quote: ... the 4 tubes were centrifuged using JA-20 rotor at 43,000 × g for 1 hour and 30 minutes in Beckman coulter® Avanti j-26 xp (Beckman, France). The pellets of the 4 tubes were preserved at −80°C before transport and metagenome sequencing and analysis.
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bioRxiv - Microbiology 2021Quote: ... and either aliquoted or concentrated by ultracentrifugation using 20% (w/v) sucrose cushions for 2 hours at 25,000 rpm in an SW41 or SW28 rotor (Beckman, Indianapolis, IN). Alternatively ...
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bioRxiv - Genomics 2021Quote: ... A second set of PacBio libraries were constructed using the manufacturer’s protocol and were size selected for 20 kb fragments on the BluePippen system (Sage Science) followed by subsequent purification using AMPure XP beads (Beckman Coulter). Sequencing was performed by the University of Delaware DNA Sequencing & Genotyping Center.
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bioRxiv - Synthetic Biology 2023Quote: ... Droplets were made by either manually pipetting 0.05-0.1 μL droplets or dispensing individual 2.5 nL - 20 nL droplets using an Echo 550 acoustic liquid handler (Beckman Coulter, USA) into 2 μL of the lipid-oil phase in 384-well microplate ...
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bioRxiv - Microbiology 2023Quote: ... The isopycnic centrifugation of DNA was performed with an initial CsCl buoyant density of 1.725 g mL-1 subjected to centrifugation at 177,000 × g for 36-40 h at 20 °C in an Optima XPN-80 ultracentrifuge (Beckman Coulter, USA). At the end of the centrifugation ...
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bioRxiv - Biochemistry 2023Quote: ... and sonicated for 10 seconds with a QSONICA Q500 sonicator equipped with a micro tip and with the amplitude set to 20% prior to analysis in a Cytomics FC500 flow cytometer (Beckman Coulter).
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bioRxiv - Plant Biology 2023Quote: ... The cAW was collected by spinning at 700 x g for 20 min at 4 ⁰C (JA-14 rotor, Beckman Coulter; Avanti J-20TM centrifuge, Beckman Coulter). vAW was filtered through a single layer of Miracloth and was combined with the cAW ...
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bioRxiv - Cell Biology 2023Quote: ... The cilia in the upper phase were sedimented by centrifugation (27,000 g, 4°C, 20 min; Beckman Coulter, Avanti JXN-26) and resuspended in Hepes-Magnesium-EGTA-Potassium (HMEK ...
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bioRxiv - Microbiology 2023Quote: ... The clarified supernatant was loaded over a 20% sucrose cushion in PBS using Ultra Clear tubes (13 x51 mm, cat n° 344057, Beckman Coulter) in a ratio of 3 to 4 of cushion and sample ...
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Expanding the toolbox for phycobiliprotein assembly: phycoerythrobilin biosynthesis in SynechocystisbioRxiv - Synthetic Biology 2023Quote: ... Ultracentrifugation was performed in a SW40 Ti swinging-bucket rotor at 130000 × g for 16 h at 20 °C (Optima L-80 XP, Beckman Coulter).
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bioRxiv - Cell Biology 2023Quote: ... 20 µL of the cell lysate in each well were transferred to a Proxi plate by a Beckman NX (Beckman Coulter). The plates were then promptly de-ionized (to prevent ESI spray instability ...
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bioRxiv - Microbiology 2023Quote: ... The normalized cultures were then centrifuged at 5030 x g and 4°C for 20 minutes on an Avanti J-26 XPI (Beckman-Coulter) centrifuge (JS 5.3 rotor) ...
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bioRxiv - Neuroscience 2024Quote: ... The same mass of total extracts was taken for a high spin at 154,000 g for 20 minutes in 4 °C using an ultracentrifuge (Beckman, TLX-120K) to obtain the supernatant soluble fraction (S1 ...
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bioRxiv - Immunology 2023Quote: ... supernatants were concentrated by ultracentrifugation through a 20% sucrose cushion (44000 rpm, 45 min) using an Optima LE-80k ultracentrifuge (Beckman Coulter), and lysed in 2x SDS sample buffer (10% glycerol ...
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bioRxiv - Genomics 2022Quote: ... samples were then transferred to a new 96-well plate at a standard volume (typically 20-30 μL) using a BioMek FXp liquid handler (Beckman Coulter). This plate was diluted to a final TIME-Seq starting concentration of 10 ng/μL using the Mantis liquid handler.
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bioRxiv - Biochemistry 2022Quote: ... to remove intact cells and the supernatant was centrifuged again at 25,000 RCF for 1 hour (Beckman Coulter JA-20 rotor). The pellet was resuspended in lysis buffer and sodium cholate was added to a final concentration of 1% and stirred at 4°C for 1 hour ...
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bioRxiv - Cell Biology 2023Quote: ... This step was followed by centrifugation at 10,000 × g for 20 min to remove cell debris using the Allegra X-30R centrifuge (Beckman Coulter, USA). The supernatant was transferred to ultra-clear thin wall tubes (Beckman Coulter ...
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bioRxiv - Plant Biology 2023Quote: ... Uptake was terminated by rapid filtering of the cells into the kill solution during 20 s centrifugation at set maximum speed (microfuge B, Beckman, USA). For dark-inactivation experiments ...
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bioRxiv - Biochemistry 2023Quote: ... to remove intact cells and the supernatant was centrifuged again at 25,000 RCF for 1 hour (Beckman Coulter JA-20 rotor). The pellet was resuspended in lysis buffer and sodium cholate was added to a final concentration of 1% and stirred at 4°C for 1 hour ...
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bioRxiv - Plant Biology 2024Quote: ... The tubes were then centrifuged for 20 min at 700 x g with slow acceleration at 4°C using a JA-14 rotor (Avanti J-20 XP centrifuge; Beckman Coulter). The resulting apoplastic wash collected was passed through a 0.22 µm membrane filter to remove bacteria and larger debris ...
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bioRxiv - Cell Biology 2024Quote: ... cells were washed with PBS at the indicated time and then stained for GPA/Band3/CD49d expression with 1:20 human BV421-anti-235a (Beckman Coulter), 1:20 human FITC-BRIC6 (IBGRL Research ...
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bioRxiv - Immunology 2024Quote: ... and adapter ligation (20°C, 60min) were followed by un-ligated adapters removal with 1.1x volume of AMPure XP beads (Beckman Coulter, USA). Libraries were amplified over 12 cycles in thermocycler using KAPA HiFi HotStart ReadyMix and 5µM Pre-LM-PCR Oligo 1&2 (Roche ...
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bioRxiv - Biophysics 2024Quote: ... cycled at 2 secs on / 2 secs off) and clarified by centrifugation at 8500 RPM for 1 hour using a fixed angle JA-20 rotor (Beckman Coulter) at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... Peptidoglycan was obtained by centrifuging for 13 min at 60,000 rpm at 20 °C (TLA100.3 Beckman rotor; OptimaTM Max ultracentrifuge Beckman, Beckman Coulter, California, USA). Pellets were washed 3-4 times by repeated cycles of centrifugation and resuspension in water ...
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bioRxiv - Biochemistry 2024Quote: ... A volume of 20 nL of the MGD dilution series was dispensed into assays wells using an Echo 650 (Beckman, USA). A volume of 20 nL of dimethyl sulfoxide was transferred into the neutral-control wells ...
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bioRxiv - Biophysics 2024Quote: ... samples were boiled for 20 minutes and centrifuged at 20,000 rpm for 45 minutes (Avanti J-26S XPI Centrifuge, Beckman Coulter, CA) to remove cellular debris ...
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bioRxiv - Biochemistry 2024Quote: ... After centrifugation at 60,000 x g for 20 min at 4°C in a swing-out rotor (MLS-50, Optima Max-XP, Beckman Coulter), the mitochondrial fraction localized in the interphase between the two sucrose layers was carefully removed with a syringe ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were fixed in 70% ethanol and incubated at −20°C for 20 minutes before being treated with RNAse A and propidium iodide (PI) for 20 minutes and run on the CytoFlex flow cytometer (Beckman Coulter), and at least 10,000 events were recorded ...
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bioRxiv - Genetics 2020Quote: ... PCR products were pooled by region of interest using 2-3 μL of each reaction and the resulting pools of products were purified using a 1X Ampure XP cleanup (Beckman Coulter). Purified libraries were pooled and submitted for paired-end 150 cycle sequencing on an Illumina MiSeq ...
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bioRxiv - Genomics 2021Quote: ... Cells were washed with 25 mm supplemented HBSS before depleting the sample of unnucleated cells using IOTest 3 Lysing solution (Beckman Coulter) following provider instructions ...