Labshake search
Citations for Beckman :
351 - 400 of 2114 citations for 3 Hydrazino 5 methyl 4H 1 2 4 triazol 4 ylamine hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2022Quote: ... The supernatant was then transferred to polycarbonate tubes and centrifuged at 100,000 × g and 4°C for 1 h in a XL-90 ultracentrifuge (Beckman Coulter) ...
-
bioRxiv - Cell Biology 2022Quote: ... This spin was repeated and the resulting supernatant was then centrifuged for 1 h at 75,000 g at 4°C (TLA-100.3 rotor or Type60 Ti rotor; Beckman Coulter, USA). The supernatant was aspirated and the membrane pellet gently resuspended in microsome buffer (10 mM HEPES/KOH pH 7.5. ...
-
bioRxiv - Molecular Biology 2023Quote: ... for 45[min at 25°C and then subjected to a sucrose cushion ultracentrifugation at 100,000[rpm for 1[h at 4°C with Optima MAX-TL ultracentrifuge and TLA-110 rotor (Beckman Coulter). The RNA fragments in the resulting pellet were recovered using TRIzol reagent (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2023Quote: ... and centrifuged at 100,000 × g for 1 h at 4 °C using a TLA-55 rotor in an Optima TLX Ultracentrifuge (Beckman Coulter). The first supernatant ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were washed twice with 200µl BD 1X Perm/Wash solution and stained 1h at 4°C with anti-HIV-1 p24 (RD1/PE, Beckman Coulter #6604667 ...
-
bioRxiv - Neuroscience 2023Quote: Monomeric wild-type α-syn was ultracentrifuged at 90,000 RCF for 1 hour at 4 °C to remove any amorphous aggregates (Beckman Coulter Optima Max-XP with MLA-130 rotor) ...
-
bioRxiv - Microbiology 2024Quote: ... 20 mL of supernatant was gently layered on top of 10mL of 20% sucrose dissolved in PBS (4°C, sterile filtered) in an ultra-clear 1×3.5′ centrifuge tube (Beckman 344058). The tubes were spun down in a SW28 rotor (Beckman ...
-
bioRxiv - Cell Biology 2024Quote: ... The samples were then centrifuged for 1 h at 100,000 g and 4°C in a TLS 55 rotor (Beckman-Coulter) with the appropriate adapters (#358614) ...
-
bioRxiv - Microbiology 2024Quote: ... Ribosomes were isolated by sucrose cushion ultracentrifuged at 100,000 rpm (543,000 × g) for 1 h at 4°C with a TLA110 rotor and an Optima MAX-TL ultracentrifuge (Beckman Coulter). Subsequently ...
-
bioRxiv - Biophysics 2024Quote: ... the clarified lysate underwent a further centrifugation step at 205,000xg for 1 hour at 4 °C (60Ti rotor, Beckman Coulter). The membrane pellet was then resuspended in the resuspension buffer ...
-
bioRxiv - Neuroscience 2020Quote: ... for further depletion of cell debris and spun at 10,000 × g for 30 min at 4°C (AH-650 rotor, Beckman Ultra-Clear Tube with 5 ml capacity). The pellet was resuspended in 100 μl PBS and considered to be the “10K pellet” (10K or 10K fraction) ...
-
bioRxiv - Neuroscience 2021Quote: ... The gradient was centrifuged at 50,000xg for 21min at 4°C (Beckman Coulter Optima MAX XP ultracentrifuge with a TL-55 rotor) ...
-
bioRxiv - Biochemistry 2021Quote: ... 4°C for 30 min using a JLA 9.1000 centrifuge rotor (Beckman). E ...
-
bioRxiv - Biochemistry 2020Quote: ... At OD600 1.2 the cells were pelleted at 4 °C (TLA10.500 (Beckman), 15 min at 5,000-8,000 rcf) ...
-
bioRxiv - Immunology 2021Quote: ... Samples in Figure 4 were collected with a CytoFlex cytometer (Beckman Coulter). Collected cells were rinsed twice with PBS and stained with Zombie-UV viability dye (BioLegend) ...
-
bioRxiv - Biochemistry 2022Quote: ... 4°C for 30 min using a JLA-9.1000 centrifuge rotor (Beckman). The pellet was resuspended in 500 mL hypotonic lysis buffer containing 10 mM Tris-HCl pH 7.8 ...
-
bioRxiv - Biochemistry 2022Quote: ... 4°C for 15 min using a JLA-9.1000 centrifuge rotor (Beckman). The pellet was resuspended in 70 mL buffer containing 50 mM Tris-HCl ...
-
bioRxiv - Cell Biology 2024Quote: Cell volumes were measured using a Coulter counter (Multisizer 4, Beckman Coulter), which is based on the principle that a cell traversing an orifice filled with electrolyte will produce an impedance change proportional to the volume of the cell due to displacement of electrolyte ...
-
bioRxiv - Cell Biology 2023Quote: ... for 20 hours at 4 °C in an ultracentrifuge (LE-80K, Beckman). The centrifuged medium was diluted with serum-free Opti-MEM supplemented with penicillin/streptomycin to a final concentration of 10% BGS and filtered using the Stericup-VP Sterile Vacuum Filtration System with a 0.1 µM PES filter (Merck) ...
-
bioRxiv - Neuroscience 2023Quote: ... 4°C for 30 minutes in an OptimaTM MAX Ultracentrifuge (Beckman, Germany). Supernatant was collected and stored at -80°C ...
-
bioRxiv - Immunology 2023Quote: ... After centrifugation at 100,000xg for 16 hat 4°C (Beckman Ti50.2 rotor), the density of each fraction was measured ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... Final ultracentrifugation step at 100,000g/1hour/4°C (Beckman AirFuge CLS Ultracentrifuge) resulted in pellets which were resuspended in the same buffer (66) ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cell count was acquired using a Multisizer 4 Coulter Counter (Beckman-Coulter) using standard protocols and a threshold of 12 to 30 μm.
-
bioRxiv - Cell Biology 2023Quote: ... Gradients were centrifuged at 200,000xg for 16 h at 4°C (Beckman–Coulter Optima™ MAX–E centrifuge ...
-
bioRxiv - Cell Biology 2023Quote: ... EVs were pelleted at 200,000xg for 60 min at 4°C (Beckman–Coulter Optima™ L–90K or Optima™ XPN–80 centrifuge ...
-
bioRxiv - Neuroscience 2024Quote: ... and spun at 4°C in a fixed vertical rotor (VTi50, Beckman) at 242,000 g for 47 min to achieve subcellular fractionation.
-
bioRxiv - Immunology 2020Quote: Intracellular cytokine production by T cells was analyzed after stimulating PBMC or whole blood (fresh or frozen) 4h at 37°C in DurActive1 tubes (Beckman Coulter), which contain PMA ...
-
bioRxiv - Biochemistry 2024Quote: ... Pellets were washed and gently resuspended in 1 mL of PBS and centrifuged at 50,000 x g for 1 h at 4°C (Beckman Optima MAX-E, TLA-55 rotor). Pellets were resuspended in 500 μl of PBS to obtain concentrated lentiviral stocks that were stored at −80°C in aliquots ...
-
bioRxiv - Biochemistry 2023Quote: ... Pellets were washed and gently resuspended in 1 ml of PBS and centrifuged at 50,000 × g for 1 h at 4°C (Beckman Optima MAX-E, TLA-55 rotor). The resulting pellets were resuspended in 500 μl of PBS to obtain concentrated lentiviral stocks that were stored at −80°C in aliquots ...
-
bioRxiv - Plant Biology 2020Quote: MP of pollen protoplasts was assessed by staining with 1 µM DiBAC4(3) for 5 min (Breygina et al. 2010) on Gallios flow cytometer (Beckman Coulter), analysis was performed with FlowJo software (TreeStar) ...
-
bioRxiv - Biophysics 2022Quote: ... and the supernatant was subjected to ultracentrifugation to separate the membrane fraction (100,000g, 1 hour, 4°C using an Optima XE-90, Beckman Coulter centrifuge). Membranes were resuspended in lysis buffer supplemented with cOmplete EDTA-free protease inhibitors (Roche) ...
-
bioRxiv - Cell Biology 2022Quote: ... A new supernatant was collected in a prechilled 1.5 ml ultracentrifuge tube and centrifuged at 100000 g for 1 h at 4°C in a TLA-55 rotor (Beckman Coulter). The resulting pellet was resuspended in the same isotonic buffer containing antiproteases and was frozen at -80°C together with the 100000 g supernatant ...
-
bioRxiv - Systems Biology 2020Quote: ... each resultant fraction underwent separate ultracentrifugation at 100,000xg for 40 minutes at 4°C (rotor TLA 120.2) in 1 ml 11×34 mm polycarbonate uncapped centrifuge tubes (Beckman Coulter 343778). In EV-enriched pooled fraction experiments ...
-
bioRxiv - Microbiology 2020Quote: ... Vesicles were obtained by centrifugation at 38,400 x g for 1 h at 4°C in an Avanti J-20XP centrifuge (Beckman Coulter, Inc). Pelleted vesicles were resuspended in 20 ml of 50 mM HEPES (pH 6.8 ...
-
bioRxiv - Microbiology 2020Quote: ... cells were fixed with 4% FA in PBS for 90 min at room temperature and immunostained for intracellular HIV-1 CA for 30 min at 4°C using KC57-FITC antibody (Beckman Coulter) diluted in 0.1% Triton X-100 ...
-
bioRxiv - Microbiology 2024Quote: ... The final Hcp1-VLPs particles were pelletized by ultracentrifugation at 274,000 ×g at 4°C for 1 h in a SW41 Ti rotor (Beckman Coulter, Inc). The final pellet was resuspended in PBS pH 7.4.
-
bioRxiv - Cell Biology 2024Quote: ... plasma samples were thawed on ice and subjected to ultracentrifugation at 100,000 g for 1 hr or 18 hr at 4 °C (TLA-55 rotor; Optima MAX-MP Tabletop Ultracentrifuge, Beckman Coulter). Pellets were washed once in 1.0 ml PBS ...
-
bioRxiv - Cell Biology 2023Quote: ... Homogenates were then transferred to a 50 ml tube and centrifuged for 10 mins at 1 000 × g at 4°C (Beckman Coulter). The resulting supernatant was then transferred to microfuge tubes for storage at -80°C as whole liver lysates or to fresh ultracentrifuge tubes for further processing.
-
bioRxiv - Bioengineering 2023Quote: ... The suspension was then ultracentrifuged (58,000 × g, 1 hr., 4 °C) in an Optima MAX-XP (Beckman Coulter, Indianapolis, IN, USA) to separate residual catalyst ...
-
bioRxiv - Biochemistry 2024Quote: ... Non-solubilized materials were removed by centrifuging the sample at 41,000 r.p.m for 1 h at 4 °C using rotor type 45 Ti in the OptimaTM L-100 XP Ultracentrifuge (BECKMAN COULTER). The resulting supernatant containing the solubilized LysP-GFP was incubated with Ni2+-NTA resin (Qiagen ...
-
bioRxiv - Microbiology 2024Quote: ... and labeled intracellularly for the structural HIV-1 p24 protein with the anti-p24 clone KC57 antibody (30 min RT followed by 30 min 4°C, Beckman Coulter). HIV-1 RNA probing was performed using the PrimeFlow RNA Assay (ThermoFisher) ...
-
Sense codon-misassociated eRF1 elicits widespread ribosome stalling and induction of quality controlbioRxiv - Molecular Biology 2024Quote: ... The mixture was layered over 900 μl of 1 M sucrose cushion 46 and ultracentrifuged at 100,000 rpm ( 543,000 × g) at 4°C for 1 h using a TLA-110 rotor and an Optima MAX-TL Ultracentrifuge (both Beckman Coulter).
-
bioRxiv - Microbiology 2024Quote: ... the supernatant was filtered through 0.45-μm syringe filters and ultracentrifuged at 100,000 ×g for 1 h at 4 °C (MLA-50 fixed angle rotor, Beckman Coulter, Germany). The supernatant was discarded ...
-
bioRxiv - Cancer Biology 2020Quote: ... 3 and 5 and counted using a particle counter (Z1; Beckman Coulter, Inc.). Experiments were carried out in triplicate ...
-
bioRxiv - Biochemistry 2020Quote: ... cells were harvested by centrifugation (6000 rpm, JA10, 20 min, 4 °C, Beckman). The pellet was re-suspended in 15-20 ml of 50 mM NaH2PO4 ...
-
bioRxiv - Cell Biology 2020Quote: ... 4 °C for 38 min with MLA-50 rotor (Beckman Coulter, California, USA). The top layer and the subsequent 10 fractions (fraction 1-10 ...
-
bioRxiv - Biochemistry 2020Quote: ... centrifuged at 90,000 rpm for 10 min at 4°C (TLA120.1 Beckman Coulter), and then polymerized by incubation at 37°C for 30 mins ...
-
bioRxiv - Biochemistry 2021Quote: ... Cells were collected after 4 days and counted by Z2 Coulter Counter (Beckman). For cell proliferation assays with fold change in cell number ...
-
bioRxiv - Microbiology 2021Quote: ... centrifuged at 42,000 rpm for 45 minutes at 4 °C (45Ti rotor; Beckman), and the resulting total membrane fraction was homogenized in TBS buffer containing 1.5% Lauryl-dimethylamine oxide (LDAO ...
-
bioRxiv - Microbiology 2021Quote: ... and were analysed with a Multisizer 4 Coulter Counter (Beckman Coulter, Brea, USA) equipped with a 30 nm aperture tube ...