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Citations for Beckman :
251 - 300 of 2114 citations for 3 Hydrazino 5 methyl 4H 1 2 4 triazol 4 ylamine hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... it was centrifuged at 110 000×g for 1 hour at 4°C (Optima TLX Ultracentrifuge, Beckman) and the fat-free infranatant was used for HSL activity measurements ...
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bioRxiv - Physiology 2023Quote: ... and centrifuged at 12,000g for 1 hour at 4°C (SW-41Ti rotor, Beckman Coulter, Pasadena, California). Six or seven fractions were collected from the top ...
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bioRxiv - Cell Biology 2023Quote: ... were spun at 100,000 x g for 1 hr at 4 oC a TLA100 rotor (Beckman-Coulter). After conversion of Ca2+-ATP-actin to Mg2+-ATP-actin ...
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bioRxiv - Biochemistry 2023Quote: ... the solution was ultracentrifuged at 91,000 g at 4 °C for 1 hour (Optima TLX Ultracentrifuge, Beckman). The concentration of the supernatant was then determined by A280 (ε280 = 5,960 M−1 cm−1) ...
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bioRxiv - Cell Biology 2024Quote: ... After ultracentrifugation at 45,000 rpm for 1 hour at 4°C in a TLS-55 rotor (Beckman), the membranes were separated into supernatant (SN ...
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bioRxiv - Biophysics 2022Quote: ... Fractions of EV-specific density were then pooled together and subsequently concentrated via ultracentrifugation (100,000 × g, 4 h, 4 °C) through a 20% w/v sucrose cushion in a SW41 rotor (Beckman Coulter, Fullerton, CA, USA). The resulting supernatant was discarded ...
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bioRxiv - Immunology 2021Quote: ... The pelleted exosomes from above supernatants were further centrifuged at 100,000g (26,450rpm) for 2 h at 4 °C (Beckman Coulter, Optima XE-90) and resuspended in PBS ...
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bioRxiv - Microbiology 2021Quote: ... Samples were centrifuged at 130,000 × g for 2 h at 4°C in a TLA100.3 rotor (Beckman Optima TLX table top centrifuge). Following centrifugation ...
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bioRxiv - Neuroscience 2021Quote: ... The post nuclear supernatant was transferred to an ultracentrifuge tube and centrifuged for 2 h at 245,000 g at 4 °C in a Beckman L90 Ultracentrifuge (Beckman Coulter, Mississauga, Canada) to pellet remaining membrane.
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bioRxiv - Immunology 2022Quote: ... Ultracentrifugation in a Beckman L7-65 ultracentrifuge at 25,000 rpm for 2 hours at 4°C using a Beckman SW28 rotor (Beckman Coulter, CA, USA) was performed to pellet the viruses through the sucrose cushion while soluble egg proteins were removed ...
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bioRxiv - Pathology 2020Quote: ... Hemocytes stained with 4’-6-diamidino-2-phenylindole (DAPI) were analyzed on a CytoFLEX flow cytometer with CytExpert software (Beckman-Coulter, USA). Plots of side scatter SSC-A versus forward scatter FSC-A were used for visualization of cell groups ...
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bioRxiv - Cell Biology 2023Quote: ... cells were blocked in 2% human serum and PBS for 30 min at 4°C and stained with DC-SIGN (Beckman Coulter #A07407), CD80 (BioLegend #305214) ...
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bioRxiv - Plant Biology 2023Quote: ... 700 μl of obtained extract was applied to a chilled sucrose density gradient and centrifuged at 4 °C for 2 hours 45 minutes at 151,000 ×g using the SW 41 Ti rotor (Beckman Culture Life Sciences). After centrifugation ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysates were loaded onto the sucrose gradients and centrifuged at 40’000 rpm for 2 h at 4°C using the SW 41 Ti rotor (Beckman Coulter, Cat# 331362). Subsequently ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were centrifuged at 100,000 × g for 2 hr at 4 °C (Type 50.2 Ti Fixed angle ultracentrifuge rotor, Beckman Coulter, Brea, CA, USA). The supernatants were aspirated ...
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bioRxiv - Genomics 2021Quote: ... The spores were washed twice with sterile ddH2O and pelleted at 3700g for 5 min at 4 °C in an Allegra X-12R centrifuge (Beckman, Brea, CA, USA). Spores were resuspended in sterile ddH2O and kept at 4°C until experiments.
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bioRxiv - Microbiology 2022Quote: ... then clarified by centrifugation at 2000 rpm at 4°C for 5 min in a refrigerated tabletop centrifuge (Beckman Coulter, Indianapolis IN, USA) to remove large debris ...
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bioRxiv - Microbiology 2024Quote: ... The supernatant was collected and treated with polyethyleneimine to a final concentration of 0.03% incubated on ice for 5 minutes and subsequently centrifuged at 10,000 rpm at 4°C for 10 minutes (Beckman coulter, JA-25.50 rotor). The supernatant was collected and powdered ammonium sulfate (approx 15 g ...
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bioRxiv - Biophysics 2021Quote: ... centrifuged (Beckman Avanti, 1500 x g, 10 min, 4 °C) and pellets were flash-frozen and stored at −80 °C.
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bioRxiv - Immunology 2022Quote: ... for 90 minutes at 4°C (Optima XE-90, Beckman-Coulter with SW 32 Ti swinging bucket rotor ...
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bioRxiv - Molecular Biology 2022Quote: ... and ultracentrifuged (60,000 rpm, 4 °C, Beckman, 70 Ti rotor) for 20 hours ...
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bioRxiv - Synthetic Biology 2020Quote: ... After clarification (20,000 rpm, Beckman JA 25.5, 30min 4°C), lysate was incubated with Glutathione S-sepharose 4B resin (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... Following centrifugation (Beckman SW40 rotor, 105,400xg, 18 h, 4°C), the heavier green band was collected and precipitated using 8% PEG6000 (Hampton Research ...
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bioRxiv - Immunology 2020Quote: ... for 90 minutes at 4°C (Optima XE-90, Beckman-Coulter with SW 32 Ti swinging bucket rotor ...
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bioRxiv - Cell Biology 2022Quote: ... at 4°C in a TLA-55 rotor (Beckman Coulter) at maximum speed (186000 g ...
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bioRxiv - Bioengineering 2023Quote: ... the centrifugation at 12,000 rpm 4 ℃ (Beckman Avanti J-26) for 30 mins was performed to remove cell debris ...
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bioRxiv - Biochemistry 2023Quote: ... 4 °C (Fixed angle Ti90 Rotor, Optima XE90, Beckman Coulter). The supernatant was loaded onto a 70 ml Q-sepharose column (XK 26/20 ...
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bioRxiv - Biophysics 2024Quote: ... 4 °C for 45 min in an ultracentrifuge (Beckman Coulter). The top fraction containing liposomes and the attached motors was harvested after the flotation assay and motor concentration was measured by GFP fluorescence.
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bioRxiv - Cell Biology 2020Quote: ... Samples were ultracentrifuged at 95,000 rpm at 4°C for 1 h and 40 min using a TLA100.2 rotor (Beckman). Supernatants were saved if needed for further analysis ...
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bioRxiv - Molecular Biology 2022Quote: ... The solution was centrifuged for 1 h at 4°C and 45,000 rpm (Type 45 Ti rotor, Beckman) and the supernatant was bound to 2 mL (CV ...
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bioRxiv - Cell Biology 2023Quote: ... 10% and 6% and ultracentrifuged at 187,000xg for 1:30h at 4°C in Sw32.1Ti rotor (Beckman Coulter). Afterwards ...
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bioRxiv - Biophysics 2023Quote: ... 1 mM DTT) and spun at 32,600 × g for 15 h at 4° in SW32 rotor (Beckman Coulter). Ribosome fractions were pooled as shown in Figure 2D ...
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bioRxiv - Developmental Biology 2023Quote: ... Peptide supernatant was cleared by ultracentrifugation at 100,000□g for 1 hour at 4□°C (Beckman Coulter, 343775), then vacuum-dried overnight.
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bioRxiv - Biophysics 2023Quote: ... the supernatant was layered onto PBS/1.5 M sucrose solution in sample/sucrose 4:1 ratio and ultracentrifuged at 264,000 × g on Optima TLA 100.3 ultracentrifuge (Beckman for 2 h at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: ... Insoluble material was removed by high speed centrifugation (30,000g, 1 hour, at 4°C, in rotor JA12, Beckman) and by 0.45 µm filtration ...
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bioRxiv - Microbiology 2021Quote: ... and extracellular vesicles including exosomes and virions were pelleted by ultracentrifugation at 141,000 g at 4 °C for 2 h in a swinging-bucket rotor (SW28; Beckman Coulter, Brea, CA, USA). The pellet was resuspended in filtered PBS and centrifuged at 18,300 g and 4°C for 10 min to remove insoluble aggregates ...
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bioRxiv - Genomics 2020Quote: ... were layered onto a 1.8M sucrose cushion and ultra-centrifuged at 24,400 rpm at 4°C for 2 hours using a SW28 swinging bucket rotor (Beckman Coulter Optimal centrifuge #L90K). The nuclear pellet was re-suspended in 2% BSA in 1X PBS ...
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bioRxiv - Microbiology 2020Quote: ... EMVs were obtained by ultracentrifugation from 4 mL of filtrate at 100,000 × g (average centrifugal force) and 4°C for 2 h with an OptimaX centrifuge (Beckman Coulter, Brea, CA, USA). The pellets were resuspended in 400 μL of Dulbecco’s phosphate-buffered saline (DPBS ...
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bioRxiv - Neuroscience 2022Quote: ... The tubes were sealed and centrifuged at 50,000 rpm at 4°C for 2 h in an OptimaTM L-90K ultracentrifuge using the 70.1Ti rotor (Beckman Coulter, Brea, CA, USA). After the centrifugation ...
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bioRxiv - Neuroscience 2022Quote: ... The samples were then spun at 31000 x g (18468 rpm) for 5 min at 4°C in a fixed-angle rotor (50.2 Ti rotor in Beckman Coulter Optima L-100 XP) with slow brake ...
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bioRxiv - Biophysics 2020Quote: ... and centrifuged at 287,582 x g (90,000 rpm) for 1 hr at 4 °C in a TLA100.2 rotor (Beckman, ref. 362046) in 11×34 mm thick-wall polycarbonate ultracentrifuge tubes (Beckman ...
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bioRxiv - Biochemistry 2022Quote: ... The crude extract was centrifuged at 200,000 g for 1 h at 4 °C (Beckman Coulter Optima LE-80K). The membrane pellet was homogenized and solubilized in 20 mM HEPES (pH 7.8 ...
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bioRxiv - Biochemistry 2021Quote: ... Reaction mixtures were incubated for 30 minutes and centrifuged at 100,000g at 4°C for 1 hr using a TLA100 fixed angle rotor (Beckman). The supernatant fraction was carefully removed ...
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bioRxiv - Microbiology 2022Quote: ... The post-nuclear supernatant was adjusted to 0.8 M sucrose and loaded in the middle of a step gradient ranging from 0.1 to 1.8 M sucrose in STKM buffer and centrifuged at 50’000 g for 1 h at 4°C in a TLA-120.1 fixed-angle rotor (Beckman Coulter). LDs formed a white cushion on top of the tube and were collected by aspiration of the upper fraction of the gradient (125 µl).
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bioRxiv - Molecular Biology 2024Quote: ... for ultracentrifugation at 100,000 × g for 1 hour at 4°C (JXN-30 centrifuge, JA-30.50 Ti Rotor, Beckman Coulter). The pellet was washed with PBS and centrifuged again at 100,000 × g for 1 hour at 4°C to remove soluble contaminant ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1 M sucrose) at 100,000 rpm (543,000 × g) and 4°C for 1 h with a TLA110 rotor (Beckman Coulter) and an Optima MAX-TL Ultracentrifuge (Beckman Coulter) ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was subjected to a second round of ultracentrifugation at 100,000 x g for 1 hour at 4°C in a Type 45Ti rotor (Beckman) to pellet cell membranes ...
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bioRxiv - Plant Biology 2023Quote: ... It was then centrifuged again at 100,000 x g for 1 h at 4 ⁰C (TLA 55 rotor, Beckman Coulter ...
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bioRxiv - Cell Biology 2023Quote: ... and 1 mM PMSF) and centrifuged for 15 hours at 4°C with 74,400 xg (SW 32.1 Ti; Beckman Coulter). 24 equal volume fractions were collected from the top and subjected to TCA precipitation ...
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bioRxiv - Plant Biology 2023Quote: ... The tubes were then centrifuged at 100,000 x g for 1 h at 4 ⁰C (TLA 55 rotor, Beckman Coulter ...