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Citations for Beckman :
251 - 300 of 2113 citations for 2' Chloro 4 5 5 dimethyl 1 3 dioxan 2 yl butyrophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... and BA.5 RBD were sorted using the MoFlo Astrios EQ Cell Sorter (Beckman Coulter). The data from FACS were gathered using Summit 6.0 and analyzed with FlowJo software version 10.8.
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bioRxiv - Microbiology 2020Quote: ... Centrifugation was performed at 120,000 × g for 2 hr (Beckman TLS 55 rotor), after which the interface between 0.25 M and 1.1 M sucrose was collected and resuspended in 1 ml 19% OptiPrep for a step gradient containing 0.5 ml 22.5% ...
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bioRxiv - Cell Biology 2022Quote: ... 2× – G2) was interrogated using a using a Gallios flow cytometer (Beckman Coulter) and subsequent data analysis with FlowJo (v.10.8.0) ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were centrifuged at 1500 rpm for 2 minutes (Beckman Allegra X15R, 524xg), supernatant was removed and tissue was carefully triturated using fire-polished glass pipettes with 2 mL pre-warmed media ...
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bioRxiv - Synthetic Biology 2021Quote: PCR products were purified with 2 volumes of RNAClean XP beads (Beckman Coulter) and then quality-checked and quantified on a Bioanalyzer (Agilent) ...
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bioRxiv - Immunology 2024Quote: ... and 2 mM DTT) and separated by ultracentrifugation at 148,900xg (Ti55 rotor, Beckman) for 1.5 hours at 4°C ...
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bioRxiv - Genetics 2024Quote: ... DNA was further purified using 2 volumes of Ampure XP beads (Beckman Coulter). DNA was extracted from 10000 undifferentiated or differentiated spermatogonia cell samples by QIAamp DNA Micro Kit (Qiagen # 56304 ...
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bioRxiv - Molecular Biology 2023Quote: ... The ribodepleted RNA was cleaned using 2× RNAClean XP beads (Beckman Coulter A63987), eluting with 20 μL DNase digestion reaction mixture (1.5 μL Turbo DNase (Invitrogen AM2238) ...
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bioRxiv - Synthetic Biology 2023Quote: ... cells were diluted 2-fold with PBS and analysed with CytoFLEX S (Beckman Coulter ...
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bioRxiv - Biophysics 2022Quote: ... 2 mM DTT) with 0.7 M sucrose in Ti-45 tubes (Beckman-Coulter). The tubes were then placed in a Ti-45 rotor and spun at 27,000 rpm (57,000 xg ...
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bioRxiv - Biophysics 2022Quote: ... 2 M urea) by resuspension and centrifugation on a JS-4.2 rotor (Beckman) at 6,000 RPM for 30 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... 2 mg/ml Proteinase K) and normal phase 2x AMPureXP (Beckman Coulter, #A63881) bead clean-up ...
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bioRxiv - Neuroscience 2024Quote: ... for 2 h using 25 ξ 89 mm polycarbonate tubes (Beckman Coulter, 355618). Pellets were resuspended in 500 μL of sterile phosphate-buffered saline (PBS ...
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bioRxiv - Neuroscience 2024Quote: ... for 2 h using 16 ξ 76 mm polycarbonate tubes (Beckman Coulter, 355603). The resulting pellets were resuspended in 250 μL of sterile PBS ...
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bioRxiv - Biochemistry 2024Quote: ... Step 2 PCR was cleaned up using Agencourt AMPure XP beads (Beckman Coulter), and final DNA concentration was measured using the Invitrogen Quant-IT dsDNA High-Sensitivity HS Assay kit quantified with a BioTek Synergy H1 Multimode Microplate Reader before pooling libraries for sequencing ...
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bioRxiv - Genomics 2024Quote: ... PCR 2 products were purified by DNA AMPure XP beads (Beckman Coulter #A63882) and eluted with 25 uL of water ...
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bioRxiv - Cancer Biology 2020Quote: ... and NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1 E7335S, Index Primers Set 2, E7500S) with Agencourt AMPure XP magnetic beads (Beckman coulter A63881) exactly as per manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... The VP2 capsid protein fractions were pooled and subjected to centrifugation at 28,000 rpm (139,065 × g) for 3 h at 4°C (Sw40Ti rotor, Beckman Coulter). The pellet was resuspended in 50 mM Tris (pH 7.4) ...
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bioRxiv - Cell Biology 2021Quote: ... and centrifuged at 97,658 x g for 3 h at 4°C using a SW41Ti rotor (Beckman & Coulter, # 331362). The polysome fractions were collected using the Foxy R1 Fraction Collector and immediately snap frozen with liquid nitrogen and stored at −80 °C ...
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bioRxiv - Plant Biology 2020Quote: ... 50 μg/ml cycloheximide) and centrifuged for 3 h at 55,000 g and 4 °C in a Type 70 Ti rotor (Beckman). The pellet was dissolved in 1% SDS ...
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bioRxiv - Molecular Biology 2022Quote: ... Gradients were spun for 3 hours at 4° C and 36,000 RPM (221,632 x g) on SW41-Ti rotor (Beckman Coulter). Monosome fractions were isolated from each sucrose gradient with Brandel Density Gradient Fractionator using 1.5 mL/min flow speed and 60% CsCl ...
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bioRxiv - Microbiology 2023Quote: ... The tube was centrifuged for 3 hours at 150’000 x g (4°C) using a SW 32.1 Ti swinging bucket (369651, Beckman Coulter). Finally ...
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bioRxiv - Neuroscience 2023Quote: ... the liposomes were isolated by centrifugation for 3 h 40 mins at 55,000 rpm at 4°C in a SW 60 rotor (SW 60 Ti, Beckman). 20 µl were harvested from the top of the gradient and mixed with 8 µl of 4x Laemmli buffer (final 62.5 mM Tris-HCl ...
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bioRxiv - Biochemistry 2022Quote: ... The membrane was solubilized for 3 hours at 4°C before separation by ultracentrifugation at 100,000 g (Ti45, Beckman) for 45 min ...
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bioRxiv - Neuroscience 2022Quote: ... supernatant was centrifuged for 3 h at 115,000 g and 4°C using the XPN-80 ultracentrifuge equipped with a Ti70 rotor (Beckman). EV pellets were resolved in 1 mL ice-cold PBS (Gibco ...
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bioRxiv - Microbiology 2024Quote: ... the supernatants were loaded onto 1 mL of 30% sucrose in PBS buffer and centrifuged at 25,000 rpm at 4°C for 3 hours using the 90AT rotor (Beckman). The pelleted viral particles were resuspended in 100 μL of PBS and fixed with formaldehyde (working concentration 0.1% ...
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bioRxiv - Neuroscience 2024Quote: ... Tubes were centrifugated at 100,000 x g (32,500 RPM) for 3 hours at 4°C using a SW55Ti rotor (Beckman Coulter). Synaptosomes enriched in the interface between 1.0-1.25 M sucrose were collected and diluted with ice-cold 0.1 mM CaCl2 ...
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bioRxiv - Cell Biology 2024Quote: ... The 100,000xg SEV-containing pellets were resuspended in 3 ml sterile cold PBS and repelleted at 100,000xg for 4 hrs in a TLA110 rotor (Beckman). SEVs were analyzed for size and number by nanoparticle tracking (ZetaView ...
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bioRxiv - Molecular Biology 2020Quote: ... bound antibodies were visualized using 100 µL of a 1:100 dilution of goat F(ab’)2 anti-human IgG coupled to phycoerythrin (Beckman Coulter, Brea, CA, USA) at 4°C for 30 min ...
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bioRxiv - Biochemistry 2020Quote: Sedimentation velocity experiments were performed using a ProteomeLab XL-1 analytical ultracentrifuge with the use of an AN60-Ti rotor and Epon-2 channel centerpieces (Beckman Coulter, Brea, CA, USA). Protein samples were diluted with SANS buffer to concentrations yielding an absorbance of 0.5 at 280 nm as measured with an 8454 UV–Vis spectrophotometer (Agilent ...
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bioRxiv - Microbiology 2023Quote: The amplicons obtained with each of the strategies (using pools 1 and 2) were pooled in each group and purified with one volume of AMPure XP beads (Beckman Coulter, Brea, CA, USA). The beads were washed with 80% ethanol ...
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bioRxiv - Immunology 2021Quote: ... The tubes were centrifuged at 10,000 g for 5 min (Microfuge 16, Beckman Coulter, Indianapolis, IN) and the collected media was combined with the previously pipetted media.
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bioRxiv - Immunology 2022Quote: ... A total of 5 × 105 living or fixed cells were analysed using a Cytoflex cytometer (Beckman Coulter Life Sciences ...
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bioRxiv - Neuroscience 2023Quote: ... for 5 min on a swing bucket centrifuge (Allegra X-12R, Beckman Coulter, Brea, CA, USA). Supernatant was decanted and the pellet was resuspended in 0.25% (vol/vol ...
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bioRxiv - Biophysics 2022Quote: ... microtubules were spun down at 200,000 × g for 5 min using an Airfuge ultracentrifuge (Beckman-Coulter) to exclude free GTP ...
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bioRxiv - Cancer Biology 2021Quote: ... decrosslinked material was purified with 2 x AMPure XP beads (Beckman Coulter, Cat #A63881) and eluted in 30 μL of 10 mM Tris-Cl ...
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bioRxiv - Neuroscience 2020Quote: ... DRG were then centrifuged for 2 min at 1000 rpm (Beckman Allegra X15R, 233xg), supernatant aspirated ...
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bioRxiv - Molecular Biology 2020Quote: ... and centrifuged for 2 hours at 55,000 rpm (TLS-55 rotor, Beckman Coulter, USA). 11x 200 μl fractions were harvested and subjected to FLAG-IP ...
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bioRxiv - Cancer Biology 2021Quote: ... resuspended in 300μl PBS/2%FBS and FACS analysed on Gallios® (Beckman Coulter) or ARIAIII (BD) ...
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bioRxiv - Biophysics 2023Quote: ... 2 mM DTT) with 0.7 M sucrose in Type 45 Ti tubes (Beckman-Coulter). Ribosomes were pelleted by centrifugation at 27,000 rpm (57,000 xg ...
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bioRxiv - Cancer Biology 2023Quote: ... concentrated for 2 h by ultracentrifugation in swing-out rotor SW-32Ti (Beckman Coulter), 26,000g ...
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bioRxiv - Physiology 2023Quote: ... Samples were ultracentrifuged at 100,000 rpm at 16°C for 2 hours (rotor: Beckman TLA-100 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Illumina RNASeq sequencing (HiSeq 2000, 2 × 75 pb) and trimming were performed by Beckman Coulter Genomics ...
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bioRxiv - Molecular Biology 2023Quote: ... and ultra-centrifuged for 2 hours at 20,000 rpm in an SW28 rotor (Beckman). Viral pellets were resuspended in 250 µL PBS and flash-frozen ...
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bioRxiv - Systems Biology 2023Quote: ... Adapter ligation was followed by 2 successive Agencourt AMPure XP bead purifications (Beckman Coulter) and library amplification by a Plasmodium-optimized KAPA PCR amplication protocol ...
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bioRxiv - Microbiology 2024Quote: ... and 2 mM dithiothreitol (DTT)) and centrifuged in an SW-41 Ti rotor (Beckman) for 2 h at 40,000 rpm ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR-2 product was cleaned up with Ampure XP bead-based reagent (Beckman Coulter). Library concentration and purity was verified using the Qubit dsDNA HS kit (Invitrogen ...
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bioRxiv - Genomics 2024Quote: ... DNA was cleaned up and concentrated with 2× AMPure XP beads (Beckman Coulter; A63882). DNA was eluted in 30µL of Buffer EB (Qiagen ...
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bioRxiv - Cell Biology 2022Quote: ... 4°C for 1 hour (JA 25.5 rotor, Beckman Coulter). The supernatant was kept and clarified by a 0.45 µM syringe filter (Pall Corporation) ...
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bioRxiv - Cell Biology 2021Quote: ... The post-nuclear supernatants were ultracentrifuged using SW41Ti rotor at 100,000 g for 3 h at 4 °C on Optima L-90K (Beckman Coulter). The fraction between 0.4–0.5 M sucrose was collected ...