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Citations for Beckman :
451 - 500 of 2113 citations for 2' Chloro 4 5 5 dimethyl 1 3 dioxan 2 yl butyrophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... the reaction was added 1 µL of 0.2µM HTO primer (5’-GTGACTGGAGTTCAGACGTGTGC*T*C) prior to PCR cycling and cleaned up using SPRISelect magnetic beads (Beckman Coulter, #B23318), 80% ethanol ...
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bioRxiv - Immunology 2024Quote: ... the supernatant was discarded and the cell pellet resuspended in 5 mL complete RPMI for counting on Vi-Cell XR automatic cell counter (Beckman Coulter). After counting ...
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bioRxiv - Bioengineering 2024Quote: Frozen TGFb1/ET1-treated and untreated hvCTS (n=7 per condition) and hvCOC (n=5 per condition) were lysed using Agencourt RNAdvance Tissue kit (Beckman Coulter) lysis buffer and homogenized at 1000 rpm for 2 min with 5 mm Stainless Steel Beads (Qiagen ...
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bioRxiv - Microbiology 2024Quote: ... 4°C for 1 h in a SW 41 Ti rotor (Beckman Coulter, Inc). The pellet was resuspended in 1 ml of PBS pH 7.4 ...
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bioRxiv - Plant Biology 2023Quote: ... at 10,000 x g for 1 h at 4 ⁰C (JA-17 rotor, Beckman Coulter ...
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bioRxiv - Biophysics 2023Quote: ... and at 4 °C for 1 hour for ultracentrifugation (70,658 g, Beckman, Type 70Ti) in vacuum ...
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bioRxiv - Developmental Biology 2023Quote: ... cleared by ultracentrifugation at 100,000Lg for 1 hour at 4□°C (Beckman Coulter, 343775), then and vacuum-dried overnight.
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bioRxiv - Biochemistry 2023Quote: ... for 1 hour at 4°C in a Type 45 Ti rotor (Beckman Coulter). The clarified lysate was then incubated with 5 mL of Ni-NTA agarose beads (Qiagen) ...
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bioRxiv - Neuroscience 2021Quote: ... 2 mL of 20% (w/v) sucrose in DPBS (-Ca, -Mg) is prepared in ultracentrifuge tubes (Beckman Coulter, 344058) to which the divided EnvA- pseudotyped viral media is added before pelleting (20,000 RPM for 2 hours at 4°C) ...
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bioRxiv - Biochemistry 2020Quote: ... The functional complex was recovered as a jelly-like pellet by centrifugation at 60,000 rpm at 25°C for 2 h in an ultracentrifuge (128,000 g in a TLA 120.2 Beckman rotor) and packed into a 1.9 mm rotor ...
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bioRxiv - Microbiology 2020Quote: ... and separated in potassium tartrate-glycerol gradient 10–35% for 2 hrs at 175,000 ×g (Beckman SW41 Ti rotor). Purified viral particles were suspended in 10 µg/ml BSA solution or 10 µg/ml CbAE-1 solution and incubated for 1 hr at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was cleared again by 2 rounds of ultracentrifugation at 100,000 rpm (358,400 x g; Beckman TLA-120.2) for 10 minutes at 0°C ...
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bioRxiv - Molecular Biology 2021Quote: ... and the tube was centrifuged for 2 hours at 190,000 x g in a swing-out rotor (SW40 Ti; Beckman Coulter ...
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bioRxiv - Microbiology 2021Quote: ... and either aliquoted or concentrated by ultracentrifugation using 20% (w/v) sucrose cushions for 2 hours at 25,000 rpm in an SW41 rotor (Beckman).
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bioRxiv - Cell Biology 2022Quote: ... milk was prevented from cooling down and was processed within 20 minutes after collection by 2 rounds of centrifugation at 3,000xg for 10 minutes at 23°C (Beckman Coulter Allegra X-12R ...
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bioRxiv - Microbiology 2022Quote: ... The pellet was resuspended in 2 ml (per fraction) of the supernatant and ultracentrifuged (Optima MAX-TL, Beckman Coulter) at 12,000 rpm (TLA55 rotor ...
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bioRxiv - Neuroscience 2023Quote: ... The tubes were centrifuged at 100,000 × g for 2 hours using a SW 55Ti swinging-bucket rotor (Beckman Coulter).
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bioRxiv - Microbiology 2023Quote: ... The filtrate was subjected to ultracentrifugation at 200,000 xg for 2 h (Optima L-100 XP ultracentrifuge; Beckman Coulter). Supernatants were discarded ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA clean-up was performed by incubating RNA solution in 2 volumes of AMPure XP Beads (Beckman Coulter, A63881) for 2 minutes at room temperature ...
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bioRxiv - Microbiology 2024Quote: Total RNA was extracted from persistently BoDV-2-infected Vero cells using Agencourt® RNAdvance Tissue kit (Beckman Coulter) and the KingFisher Flex system (Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2024Quote: ... A 2 µg/µL BSA solution (in water) was transferred from a 384-well polypropylene 2.0 Plus Source microplate (Beckman) using the 384PP_Plus_BP fluid type into a 384-well destination plate (BioRad ...
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bioRxiv - Molecular Biology 2024Quote: ... were generated by transient calcium phosphate transfection of HEK-293T cells and concentrated from culture supernatant by ultracentrifugation (2 hours at 128,000xg; Ultraclear Tubes; SW28 rotor and Optima L-100 XP Ultracentrifuge; Beckman). The viruses were suspended in cold sterile PBS and titrated by transduction of Jurkat cells for 48 hours ...
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bioRxiv - Neuroscience 2021Quote: ... Before FACsorting we added the 5 ul of the cellular death dye Draq7TM (Viability dye, Far-red DNA intercalating agent, Beckman Coulter, #B25595). The suspension was sorted on an Astrios II cell sorter (Beckam Coulter) ...
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bioRxiv - Plant Biology 2021Quote: ... cesium chloride continuous density gradient in resuspension buffer and centrifuged at 34,000 m for 5 hours at 20 °C using an SW41 Ti rotor (Beckman Coulter, Brea, CA). The virus particles were observed in the lower of two pale white bands in the gradient ...
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bioRxiv - Immunology 2021Quote: ... were loaded onto 5-25% (w/v) linear sucrose gradients and centrifuged in a Beckman Optima L-90K Ultracentrifuge (Beckman SW40 rotor) at 40,000 rpm for 3 h 15°C ...
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bioRxiv - Genomics 2021Quote: ... with 5 PCR reactions per column then pooled together and cleaned up a second time with 1.6X SPRI beads (Beckman Coulter, Brea, California). The CHEQ-seq vector containing a random 17 bp BC upstream of the synthetic intron was linearised via inverse PCR with primers “CHEQseq_lin_A_For” and “CHEQseq_lin_A_Rev” or “CHEQseq_lin_B_For” and “CHEQseq_lin_B_Rev” ...
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bioRxiv - Neuroscience 2022Quote: ... was loaded onto a sucrose gradient made up of 5 ml 1.2 M sucrose on the bottom and 5 ml of 0.8 M sucrose at the top in thin wall ultracentrifugation tubes (Beckman Coulter, Cat # 344059). The loaded gradient was ultracentrifuged at 69,150 x g for 1 h at 4 °C (SW 41 Ti Swinging-Bucket Rotor ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were stained with 5 μg/ml PI (ST1569-10mg, Beyotime) and immediately analyzed using the CytoFLEX flow cytometer (Beckman Coulter, USA). Tumor cells were distinguished based on GFP positivity or CD45 negativity ...
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bioRxiv - Plant Biology 2023Quote: ... and 2 mL of 5% solution in a 14 x 89 mm (13.2 mL) thin walled ultra-clear centrifuge tubes (Beckman Coulter, Cat. # 344059). Approximately 250 µl of crude EV sample resuspended in 20 mM Tris-HCl pH 6.0 was slowly dispensed on top of the column and centrifuged at 100,000 x g for 17 h at 4 ⁰C (SW 41 Ti rotor ...
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bioRxiv - Cancer Biology 2023Quote: ... Conditioned media were centrifuged at 1000 rpm for 5 min to remove cell debris and then either added to Ultra-Clear Quick-Seal ultracentrifuge tubes (Beckman Coulter 246.344322), 100 kDa molecular weight cut-off centrifugal filters (MWCO ...
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bioRxiv - Plant Biology 2024Quote: ... The cell lysate was centrifuged at about 16000 g for 10 min at 5 °C (Avanti J-265 XP, Beckman Coulter, USA). The starch pellet was recovered ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 20 nL/well of library compounds were transferred to the columns 5-48 of each plate by using Echo 525 acoustic dispenser (Beckman Coulter, CA). The microplates were incubated at room temperature for 10 minutes and then loaded on the FLIPR Penta High-Throughput Cellular Screening System (Molecular Devices ...
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bioRxiv - Developmental Biology 2023Quote: ... and centrifuged at 40,000 rpm for 1 h at 4°C (Beckman SW-41Ti rotor). Supernatants were discarded ...
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bioRxiv - Genomics 2020Quote: ... RAD library quality was inspected on a 2% agarose gel before undergoing a final AMPure XP (Beckman Coulter, Indianapolis, IN) purification and quantification on a Qubit 2.0 Fluorometer (ThermoFisher Scientific ...
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bioRxiv - Genomics 2020Quote: ... The crude nuclei were stained with 4,6-diamidino-2-phenylindole and loaded into a flow cytometer (Beckman Coulter MoFlo XDP). Nuclei were purified by flow sorting and washed in accordance with (70) ...
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bioRxiv - Molecular Biology 2020Quote: ... 100 ug/ml cycloheximide) and macromolecular complexes were separated by velocity gradient ultracentrifugation (SW41Ti – Beckman, 39,000 rpm, 2 hr, 4oC). Fractions were collected using Biocomp Gradient Station and pooled to obtain separate monosomal and polysomal fractions ...
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bioRxiv - Cell Biology 2021Quote: ... Collected FT were then subjected to ultracentrifugation for 2 hours at 200 000g using the TLA 45 rotor (Beckman Coulter) and resuspended in 20μL of Laemmli 1X (BioRad) ...
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bioRxiv - Biochemistry 2021Quote: ... Equal parts of 30% and 10% iodixanol gradient solutions were layered on the mitochondrial fraction and then samples were centrifuged at 50,000 x g for 2 h (Beckman SW41TI). The mitochondrial layer was collected ...
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bioRxiv - Cell Biology 2021Quote: Microtubules were sedimented onto poly-L-lysine coated coverslips by centrifuging through 20% glycerol BRB80 cushion at 4700 rpm for 2 h in S6096 rotor (Beckman). Coverslips were subsequently fixed with 20°C methanol ...
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bioRxiv - Synthetic Biology 2021Quote: ... The collected supernatants were centrifuged at 300,000 x g for 2 hr with Optima MAX-XP ultracentrifuge with SW 55 Ti Swinging-Bucket Rotor (Beckman Coulter). The pellets consisting of the cell membrane fractions were resuspended into 10 mL of the same glycan containing media and incubated in the anaerobic chamber at 37°C for 16 hr ...
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bioRxiv - Cell Biology 2020Quote: ... The supernatant was then overlaid onto a 2 ml 60% iodixanol cushion and centrifuged at 100,000 × g (SW32 rotor, Beckman Coulter) for 18h ...
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bioRxiv - Molecular Biology 2022Quote: ... Single-cell clones were then assessed for binding to His-tagged SARS-CoV-2 Spike using a Cytoflex S (Beckman) cell analyser ...
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bioRxiv - Developmental Biology 2020Quote: ... Virus-containing supernatant was collected for two consecutive days and concentrated by ultracentrifugation for 2 hours at 19,400 rpm using an Optima L-80 XP Ultracentrifuge (Beckman Coulter).
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bioRxiv - Microbiology 2021Quote: Total RNA was isolated from Vero E6 and Caco-2 cells 48 hours after infection using RNAdvance Viral Kit (Beckman) using a KingFisher and RNeasy Mini Kit (Qiagen) ...
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bioRxiv - Cancer Biology 2020Quote: ... samples were suspended in FACS buffer (PBS, 20 mM EDTA pH 8.0, 2% FCS) and acquired using a CyAn ADP9 flow cytometer (Beckman Coulter), FACS LSRII Fortessa or FACSymphony (both BD Biosciences) ...
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bioRxiv - Microbiology 2021Quote: ... pspAX.2 and oligonucleotides were incubated for 48 h and GFP expression level was analyzed by CytoFLEX flow cytometer (Beckman).
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bioRxiv - Neuroscience 2022Quote: ... new medium was added for another round of production and supernatant was filtered (0.45 μm filter) and centrifuged for 2 hours at 19400 rpm (SW28 Beckman rotor). The pellets were suspended in phosphate buffer saline (PBS) ...
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bioRxiv - Microbiology 2022Quote: ... was added before ultracentrifugation at 20,000 × g for 2 hours at 10°C in an Optima L-90K Ultracentrifuge with the SW32Ti rotor (Beckman Coulter). Supernatants were carefully discarded by aspiration and the 6 phage pellets were resuspended with 0.1 ml of TM buffer (50 mM Tris-HCl ...
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bioRxiv - Synthetic Biology 2021Quote: Reactions were assembled in a final volume of 2 μL using a Labcyte Echo® 550 (Beckman Coulter, Brea, CA) via a two-step strategy ...
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bioRxiv - Neuroscience 2021Quote: ... The resulting homogenate was mixed with 6 mL sucrose (2 mol/L) and 2.5 mL CaCl2 (0.1 mmol/L) in an ultra-clear centrifuge tube (#344059, Beckman Coulter, USA). Then ...