Labshake search
Citations for Beckman :
51 - 100 of 2113 citations for 2' Chloro 4 5 5 dimethyl 1 3 dioxan 2 yl butyrophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... Purification was done by ultracentrifugation for 2 h at 154,000xg at 4°C in a SW41 rotor (Beckman) using a two-step sucrose gradient (20%/50% w/v in HNE) ...
-
bioRxiv - Cell Biology 2020Quote: ... the cleared supernatant was ultracentrifuged at 47,000 g for 2 hours at 4 °C using the JA25.50 rotor (Beckman). The virus was resuspended overnight in 180 μL D10 medium at 4 °C ...
-
bioRxiv - Cell Biology 2021Quote: ... 4 °C for 2 h in an Optima L-100XP ultracentrifuge (with SW 55 Ti rotor, Beckman Coulter). Fractionation was done manually by collecting every 200 μl volume from the top till the bottom of each centrifuge tube ...
-
bioRxiv - Molecular Biology 2023Quote: ... Digested extracts were then loaded on 1 M sucrose cushions 116 and centrifuged at 70,000 rpm for 2 hours at 4°C in a TLA100.4 rotor (Beckman). Ribosome pellets were resuspended in 300 µl of TRIzol reagent (Invitrogen) ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... Digested-ligated products were purified using 1:5:1 ratio of AMPure XP beads (Beckman Coulter, USA). Size selection was performed using a BluePippin to retain total fragment sizes of 250-500bp and were purified using the QIAquick Gel Extraction Kit (Qiagen ...
-
bioRxiv - Microbiology 2020Quote: MT-2 cells were collected and washed three times by pelleting at 200 x g for 5 min at 4°C in an Allegra centrifuge (Beckman) and resuspending cells in ice-cold PBS ...
-
bioRxiv - Cell Biology 2022Quote: ... brought up to 3 ml using sterile 20 mM Tris-HCl pH 7.5 and pelleted at 40,000g and 4°C for 60 minutes (TLA100.3 rotor, Optima Max-XP ultracentrifuge, Beckman Coulter, Inc.). Finally ...
-
bioRxiv - Molecular Biology 2020Quote: ... The following day the viral pellet was resuspended in 5 ml PBS and centrifuged at 20,000 rpm for 90 mins at 4°C (Optima XL-100K, Beckman Coulter). The pellet was then resuspended in the required volume of PBS before being aliquoted and stored at −70°C.
-
bioRxiv - Genomics 2023Quote: ... The suspension was incubated on ice for 5 min and centrifuged at 20,000 ×g (10 min, 4 °C, JA-20 rotor, Avanti J-26 XP, Beckman Coulter). The pellet was re-centrifuged for 5 min and both supernatants were combined into a new tube ...
-
bioRxiv - Neuroscience 2022Quote: ... trypsin-dissociated cells on 96-well plates were centrifuged at 1,000g for 5 min at 4°C using a swinging-bucket rotor for microplates (Beckman S6096) in an Allegra X-30R centrifuge (Beckman) ...
-
bioRxiv - Cell Biology 2024Quote: ... The step gradients were centrifuged for 5 h at 132.000 x g at 4 °C (SW60 Beckman and Coulter, # 335649). Subsequently ...
-
bioRxiv - Immunology 2021Quote: ... CD38 APC-AF700 (clone LS198-4-3; Beckman Coulter), CD19 APC-Cy7 (clone SJ25C1 ...
-
bioRxiv - Molecular Biology 2024Quote: ... bottom layer was made by diluting OptiPrep™ solution with clarified HM or digesta sample (2.25 mL OptiPrep™ + 0.75 mL sample = 3 mL) into a 5 mL open-top thin wall ultra-clear tube (Beckman Coulter). A discontinuous gradient was made with 40% (w/v) ...
-
bioRxiv - Pathology 2024Quote: ... with primers located in the U3 region of the 5’ and 3’ LTRs (Figure S1) and purified using “1X AmpureXP beads” (Beckman Coulter). ONT (Oxford nanopore technologies ...
-
bioRxiv - Microbiology 2024Quote: ... 21000 rpm for 5 h (Beckman SW41Ti). The band of the expected density was pooled using a syringe and the buffer was exchanged to the E-buffer using 100kDa VivaspinTM columns (Cytiva) ...
-
bioRxiv - Cell Biology 2020Quote: ... Lysates were loaded on the top of the gradient and centrifuged at 260,000 × g and 4 °C for 2 h using SW 41 rotor (Beckman). Collection of polysomal fractions were done using Piston Gradient Fractionator (BioComp) ...
-
Human Sex Matters: Y-linked lysine demethylase 5D drives accelerated male osteogenic differentiationbioRxiv - Cell Biology 2021Quote: ... Virus was transferred into conical tubes and spin at 20000rpm for 2 hours at 4°C in (Beckman TST2838) swinging bucket rotor ...
-
bioRxiv - Biochemistry 2021Quote: ... 2 mM DTT) by being centrifuged at 73000 rpm for 45 minutes at 4°C (Beckman Coulter TL120.2 rotor). The mitoribosomal pellet was subsequently resuspended in ribosome resuspension buffer (25 mM HEPES-KOH pH = 7.5 ...
-
bioRxiv - Neuroscience 2022Quote: ... and centrifuged at 24,000 rpm for 2 h at 4°C using a SW-Ti32 swinging bucket (Beckman Coulter). The upper layer was discarded ...
-
bioRxiv - Cell Biology 2021Quote: ... Pellets were resuspended in 0.9% NaCl and spun at 110,000 × g for 2 hours at 4°C in an XPN-80 ultracentrifuge equipped with a Type 50.4 Ti rotor (Beckman Coulter ...
-
bioRxiv - Microbiology 2021Quote: ... The VLPs were concentrated by ultracentrifugation for 2 h at 31,000 rpm at 4 °C (Beckman SW-31Ti rotor), and then resuspended in 500 μL of Ex-Cell 405 medium ...
-
bioRxiv - Neuroscience 2023Quote: ... Samples were then centrifuged at 82,600×g at 4°C for 2 h (Beckman ultracentrifuge optima L-100 XP). After the first ultracentrifugation ...
-
bioRxiv - Plant Biology 2023Quote: ... The supernatant from this step was decanted into the new tubes and centrifuged at 39,800 x g for 2 h at 4 ⁰C (JA-17 rotor, Beckman Coulter ...
-
bioRxiv - Molecular Biology 2023Quote: ... 1-1.5 μg of total protein was loaded onto a 10% to 50% sucrose gradient and centrifuged at 36,000 rpm for 2 hours at 4°C using an SW41 rotor (Beckman). Polysome profiles were visualized ...
-
bioRxiv - Cancer Biology 2024Quote: ... 100 μg/mL cycloheximide) for 2 h at 40,000 g at 4 °C using an SW41 rotor (Beckman Coulter). Gradients were fractionated using Teledyne Isco Tris Peristaltic Pump and fractions were collected and pooled according to the UV trace ...
-
bioRxiv - Cell Biology 2024Quote: ... samples were ultracentrifuged at 160,000 xg for 2 h at 4 °C in ultracentrifuge tubes (Beckman Coulter Cat #328874). The resulting pellet contained the insoluble AMB-1 cell fraction and the supernatant contained the soluble fraction ...
-
bioRxiv - Cell Biology 2024Quote: ... The supernatant was then subjected to ultracentrifugation at 100,000 x g for 2 h at 4 °C to sediment small EVs (Optima L-80K ultracentrifuge, Beckman Coulter ...
-
bioRxiv - Genetics 2023Quote: ... Fifteen A260 units of WCEs were layered on a pre-chilled 10-50% sucrose gradient and centrifuged at 40,000 rpm for 2 h at 4°C in a SW41Ti rotor (Beckman). Gradient fractions were continuously scanned at A260 using the BioComp Gradient Station and polysome to monosome (P/M ...
-
bioRxiv - Cancer Biology 2023Quote: ... the obtained supernatant was filtered through a 0.22 μm filter before ultracentrifugation at 150,000 g for 2 h at 4 °C (Beckman Coulter). The nsEV-containing pellet was resuspended in phosphate buffer saline (PBS).
-
bioRxiv - Molecular Biology 2023Quote: ... The gradients were centrifuged at 38,300 rpm for 2 hours and 45 min at 4 °C in a SW-40Ti (Beckman) rotor using an Optima XE-100 Ultracentrifuge (Beckman) ...
-
bioRxiv - Molecular Biology 2023Quote: ... The gradients were centrifuged at 38,300 rpm for 2 hours and 45 min at 4 °C in a SW-40Ti (Beckman) rotor using an Optima XE-100 Ultracentrifuge (Beckman) ...
-
bioRxiv - Plant Biology 2023Quote: ... Extract was then centrifuged at 40 000 rpm for 2 hours at 4°C using the 50Ti rotor (Beckman). A protocole previsouly used in the laboratory and described in Maclot et al ...
-
bioRxiv - Molecular Biology 2024Quote: ... were layered on top of each sucrose gradient and centrifuged (41,000 rpm for 2 hours at 4°C) in an SW41 Ti rotor (Beckman). UV profiling (254 nm ...
-
bioRxiv - Biophysics 2021Quote: ... and the fractions containing the protein of interest were combined and concentrated to 2mL at a final concentration of 3.85 mg/mL by repeated centrifugation in an Amicon Ultra Centrifugal Filter at 4k rpm for 5 min at 4 °C (Beckman SX4250). The concentrated protein was incubated overnight with TEV (1:50 mass ratio ...
-
bioRxiv - Biochemistry 2020Quote: ... cells were centrifuged at 4 ℃ for 5 minutes at 300g and suspended in HBSS and analyzed with a flow cytometer (CytoFLEX, Beckman Coulter). Mean fluorescence intensity was calculated for each group.
-
bioRxiv - Cell Biology 2022Quote: ... After ultracentrifugation at 200,000 x g for 5 h at 4°C (SW55Ti rotor in a XL-90 Beckman Coulter centrifuge), fractions containing purified EVs or purified phages were extracted from the top following the scheme in Supp ...
-
bioRxiv - Cell Biology 2024Quote: ... We loaded 2 mL of the homogenate mixture on top of a 3.92 ml 62% sucrose in MAB cushion in an Ultra-Clear 5/8” X 4” Beckman Coulter tube (344061, Beckman Coulter). We added the remaining sucrose in MAB solutions ...
-
bioRxiv - Neuroscience 2024Quote: ... 20.000 rpm for 2 hours (Beckman). Collected viral particles were then suspended in DMEM and stored to –80°C ...
-
bioRxiv - Microbiology 2020Quote: ... Excess dye was removed by ultracentrifugation of the virus through a 10% sucrose cushion for 2 h at 107,000 × g and 4°C using an SW41 rotor (Beckman Coulter). Labeled virus pellets were resuspended at a viral protein concentration of 1 mg/ml ...
-
bioRxiv - Biophysics 2021Quote: ... The unbound cyt c was separated from liposome-bound cyt c by ultracentrifugation at 435,000 g for 2 hours at 4 °C in an Optima TLX ultracentrifuge with a TLA-100 rotor (Beckman Coulter). Immediately after the ultracentrifugation ...
-
bioRxiv - Genomics 2021Quote: ... lentiviral supernatant was ultracentrifuged at 23,000 RPM at 4 °C for 2 hours in an SW 32 Ti swinging bucket rotor (Beckman Coulter). After centrifugation ...
-
bioRxiv - Microbiology 2022Quote: Extracellular HSV-1 or HSV-2 particles were harvested by sedimentation at 12,000 rpm for 90 minutes at 4°C (Type 19 rotor, Beckman-Coulter) from the medium of BHK-21 cells (40 x 175 cm² flasks ...
-
bioRxiv - Cell Biology 2022Quote: ... and concentrated by 20% sucrose gradient ultracentrifugation in an Optima XPN-100 ultracentrifuge at 26,000 rpm at 4°C for 2 hours in a SW32 Ti swinging bucket rotor (Beckman-Coulter). Viral particles were resuspended in PBS and stored at −80°C.
-
bioRxiv - Microbiology 2021Quote: ... Concentrated supernatants were then subjected to ultracentrifugation at 160,000 x g for 2 hours at 4°C using a Beckman Ti 45 rotor (Beckman, Germany). Supernatants were completely removed and the pellet containing the crude MV fraction was resuspended in 400 μL of chilled PBS ...
-
bioRxiv - Neuroscience 2022Quote: ... The gradient was subjected to high-speed centrifugation at 100,000 x g for 2 h at 4° C using a SW41Ti rotor (Beckman Coulter). 12 fractions of 1ml each were collected from the top of the gradient ...
-
bioRxiv - Neuroscience 2022Quote: ... Lentiviral supernatant was collected 48 hours after transfection and centrifuged for 2 hours by ultracentrifugation at 25,000 RPM at 4°C (Beckman Coulter). Pellets were resuspended in DPBS and frozen in -80°C until used ...
-
bioRxiv - Molecular Biology 2021Quote: ... cell lysates were cleared by centrifugation at 4 °C for 5 minutes at 16000 x g and loaded onto ice-cold 15-50 % sucrose-CHX gradients and ultracentrifuged at 4 °C for 2 hours at 274 000 x g in a SW-41Ti rotor (Beckman). Gradients were fractionated and monitored at an absorbance of 254 nm with a fraction collector (BioComp Instruments ...
-
bioRxiv - Molecular Biology 2020Quote: ... Centrifugation was conducted at 40,000 rpm for 2 hours at 4°C in a SW-41 Ti rotor (Beckman Coulter), and fractions were collected from the top of the gradient (#1 to #20) ...
-
bioRxiv - Biochemistry 2020Quote: ... Protein-liposome complexes were separated from unbound protein by centrifugation (2 h at 45.000 rpm, 4 °C, MLS50 rotor -Beckman Coulter) in the density gradient ...
-
bioRxiv - Cell Biology 2023Quote: ... Each fraction was subjected to a washing step in ice-cold PBS at 100,000 × g at 4°C for 2 h using a SW28Ti rotor (Beckman Coulter). The pelleted EVs were resuspended in ice-cold PBS ...