Labshake search
Citations for Beckman :
1651 - 1681 of 1681 citations for Urothelial Cell Culture Supplements since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... The cells were then incubated for 30 minutes with the following antibodies: anti-HIV-1 core antigen (p24)-RD1 (Beckman Coulter catalog # 6604667), anti-TNFα PE/Dazzle 594 (BioLegend catalog # 502946) ...
-
bioRxiv - Biochemistry 2023Quote: ... Expression was continued for 7h and the cells harvested by centrifugation at ∼6250g for 15 min at 4°C (Beckman Coulter centrifuge, JLA8.1000).
-
bioRxiv - Microbiology 2023Quote: ... cells were pelleted by centrifugation at 6,000 ×g at 4 °C for 15 min with ALLEGRA X-30R (Beckman Coulter, Brea, CA, USA). The supernatant was centrifuged at 15,000 ×g at 4 °C for 15 min with Sorvall LYNX4000 (Thermo Scientific ...
-
bioRxiv - Plant Biology 2023Quote: ... The disrupted cells were subjected to ultracentrifugation at 4°C and 235000 g for 1h (Beckman Optima™ L-80 XP, rotor Ti45). The resulting supernatant was loaded onto an Äkta purifier system (GE HealthCare ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... Five thousand cells from each of the 300 samples were analyzed in 96-well plates in a CytoFLEX Platform flow cytometer (Beckman Coulter, California, USA) at the Feldan Therapeutics facility (Quebec ...
-
bioRxiv - Cancer Biology 2023Quote: ... Gating strategies were applied accordingly to remove any cell doublets and fluorescent signal was analysed using Kaluza software (Beckman Coulter Life Sciences, U.S.) (Supplementary Figure 1).
-
bioRxiv - Cell Biology 2023Quote: ... The stained cells were resuspended in PBS and analyzed using CytoFLEX S or Galios® flow cytometers (Beckman-Coulter Corp., Hialeah, FL, USA). Data analyses were performed using FlowJo version 10.
-
bioRxiv - Biochemistry 2023Quote: ... Expression was continued for 16h and the cells harvested by centrifugation at ∼6250g for 15 min at 4°C (Beckman Coulter centrifuge, JLA8.1000). The RING Zn-finger domains from both RING1B and PCGF4 subunits were cloned into a modified polycistronic expression vector (pET29a ...
-
bioRxiv - Developmental Biology 2024Quote: ... under a fluorescent dissecting scope and the remaining roots were subjected to 1.5 hours of protoplasting55. Each of the cell types with positive GFP/mCherry expression (Fig. S2) were collected by FACS (Beckman Coulter MoFlo XDP) in 15 mL tubes (Falcon ...
-
bioRxiv - Cancer Biology 2024Quote: ... ATP production was detected with a CellCounting-Lite® 2.0 Luminescent Cell Viability Assay kit (Vazyme Biotech, Nanjing, China) by a plate reader (Beckman Coulter, Krefeld, Germany) according to the manufacturer’s instructions.
-
bioRxiv - Immunology 2019Quote: ... For patients enrolled in the study, the haemoglobin and red cell indices (MCV, MCH were determined and MCHC) using an automated analyser (Beckman Coulter, Durban, South Africa). CD4-cell counts were assessed using BD FACS Count (BD Biosciences ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... We performed a two-step nuclear DNA Content Analysis using Partec Buffer (de Laat et al., 1987) and DAPI fluorochrome through a Cell Lab Quanta SC flow cytometer (Beckman Coulter, Fullerton, CA, USA) equipped with a mercury lamp following the protocol of Doležel et al ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... The red blood cell count of birds (number of red blood cells per ml of blood) was quantified immediately before the blood meal using flow cytometry (Beckman Coulter Counter, Series Z1). One hour after the last injection ...
-
bioRxiv - Cancer Biology 2022Quote: Genomic DNA was isolated from all TP53-KO minor cells treated in F10 matrigel assays using the Agencourt DNAdvance Genomic DNA Isolation Kit (Beckman Coulter, Cat. No. A48705), and the TP53 locus was amplified using the following primers ...
-
bioRxiv - Biochemistry 2020Quote: ... Cell lysates were collected and RNA was isolated using the Biomek FxP and the RNAdvance Tissue Isolation kit (Beckman Coulter, USA, p/n A32646). The resulting RNA was quantified using the Nandrop 8000 (Nanodrop ...
-
bioRxiv - Microbiology 2021Quote: ... 50 μL of each cell sample was mixed with 1 μL of KC57-FITC antibody that identifies the HIV-1 Gag and p24 (Beckman Coulter Life Sciences, USA) and incubated at 4°C for 20 min ...
-
bioRxiv - Systems Biology 2022Quote: ... supernatant containing metabolites extracted from 1.1×109 cells and 11 spike-ins were aliquoted into 1,000 vials using an automated liquid handler (Biomek 4000, Beckman Coulter, Inc., Brea, California, USA), with 5 μL solution per tube ...
-
bioRxiv - Biophysics 2023Quote: ... of 0.6 at 260 nm were loaded into cells fitted with sapphire windows and 12 mm double channel epon charcoal centerpieces (Beckman Coulter, Indianapolis, IN, USA). Data were collected in intensity mode at 260 nm ...
-
bioRxiv - Biochemistry 2023Quote: ... cells were treated with the drugs (20 µM to a suspension at 500,000 cells/mL in PBS containing 2 % FBS at 37°C) and directly analyzed by flow cytometry (Gallios, Beckman-Coulter, 405/488 nm laser) over 2 h ...
-
bioRxiv - Cell Biology 2023Quote: ... where OD550 = 1 corresponds to ∼ 1 × 106 cells ml -1 (David L. Spector, 1998) or a Z1 Coulter Counter (Beckman Coulter Inc., Indianapolis, Indiana). Cultures were generally used during the log phase of cell growth (cell density 2-4 × 105 cells ml-1) ...
-
bioRxiv - Genomics 2023Quote: ... Cell pellets were processed for genomic DNA extraction following the magnetic bead-based Genfind V3 DNA isolation protocol (Beckman Coulter Life Sciences Cat# C34881).Next-generation sequencing was performed on the Illumina MiSeq platform ...
-
bioRxiv - Immunology 2023Quote: ... Suspended cells were incubated in the dark for 30 min at room temperature with CD66b-APC-Alexa Fluor 750 (Beckman Coulter Life Sciences, B08756) for the detection of neutrophils ...
-
bioRxiv - Cell Biology 2023Quote: ... where OD550 = 1 corresponds to ∼ 1 × 106 cells ml -1 (David L. Spector, 1998) or a Z1 Coulter Counter (Beckman Coulter Inc., Indianapolis, Indiana). Cultures were generally used during the log phase of cell growth (cell density 2-4 × 105 cells ml-1).
-
bioRxiv - Bioengineering 2024Quote: Cell culture medium was centrifuged at 200 x g for 10 min and recombinant mAb in cell-free supernatant was measured by fluorescence polarisation using ValitaTM TITER rapid high-throughput IgG quantification assay microplates (Beckman Coulter,High Wycombe, U.K.), according to manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2024Quote: ... Cell extracts were clarified by centrifugation and were sedimented by ultracentrifugation for 120 min at 70,000 rpm (Beckman Optima Max-TL, TLA-110 rotor) at 4°C in a 50% sucrose cushion buffered in 50 mM Tris-acetate ...
-
bioRxiv - Biophysics 2023Quote: ... Cells were harvested by centrifugation at 8000 g at 4°C for 20 min (Avanti® J-E centrifuge from Beckman Coulter, rotor: JLA 16.250).
-
bioRxiv - Neuroscience 2020Quote: ... for further depletion of cell debris and spun at 10,000 × g for 30 min at 4°C (AH-650 rotor, Beckman Ultra-Clear Tube with 5 ml capacity). The pellet was resuspended in 100 μl PBS and considered to be the “10K pellet” (10K or 10K fraction) ...
-
bioRxiv - Biochemistry 2019Quote: ... Resuspended cells were lysed by French Press (8,000 psi, 4°C, 2 passages) and lysates were centrifuged (Beckman 60Ti rotor, 30 min, 50,000 rpm, 4°C). Pellets were resuspended in 100 ml of buffer B (PBS [140 mM NaCl ...
-
bioRxiv - Microbiology 2023Quote: ... another 2 mL of staining bacterial samples was analyzed with a flow cytometry to check cell viability (Beckman CytoFLEX FCM, USA, Channel FL2-A: PE-A).
-
bioRxiv - Biochemistry 2020Quote: ... Protein expression was induced with 0.4 mM Isopropyl β-D-1-thiogalactopyranoside and cells were grown at 37 °C overnight before harvesting by centrifugation at 4000 rpm (Beckman Coulter Avanti JXN-26; JLA8.1 rotor) at 10 °C for 10 min.
-
bioRxiv - Biochemistry 2019Quote: ... Protein expression was induced with 0.4 mM Isopropyl β-D-1-thiogalactopyranoside and cells were grown at 25 °C overnight before harvesting by centrifugation at 4000 rpm (Beckman Coulter Avanti JXN-26; JLA8.1 rotor) at 4 °C for 15 min.