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Citations for Beckman :
1501 - 1550 of 1681 citations for Urothelial Cell Culture Supplements since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Cell sorting was performed at the University of Colorado Cancer Center Flow Cytometry Shared Resource using a MoFlo XDP cell sorter equipped with a 100 micron nozzle (Beckman Coulter). The sorting strategy excluded debris and cell doublets by light scatter and dead cells by DAPI (1 μg/ml) ...
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bioRxiv - Cell Biology 2022Quote: Live Jurkat E6.1 cells were stained with Laurdan dye as detailed above and analyzed by flow cytometry using a Cytoflex S (Beckman Coulter). Cells were resuspended in HBSS without Ca2+ and Mg2+ (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... Several clonal lines were obtained by transduction of the RevVNP cell line with the mentioned particles and flow cytometry sorting (Beckman Coulter). For reproducibility reasons ...
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bioRxiv - Immunology 2019Quote: ... Naïve B cells (CD19+ CD27− IgD+) and unswitched memory B cells (CD19+ CD27+ IgD+) were obtained by FACS sorting on a MoFlo Astrios (Beckman Coulter). Purified samples were pelleted and stored at −80°C.
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The antibiotic bedaquiline activates host macrophage innate immune resistance to bacterial infectionbioRxiv - Immunology 2019Quote: ... Cells were detached and were fixed with 4% paraformaldehyde and the fluorescence was analyzed using a CytoFLEX Flow Cytometer (Beckman Coulter).
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bioRxiv - Developmental Biology 2019Quote: Detection of apoptotic TCam-2 cells was performed 48 h after transfection of TCam-2 cells with control and KIF18A siRNA constructs using an Annexin V-FITC Apoptosis Detection Kit (Beckman Coulter), according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2019Quote: Cells were stained for 30 min (minutes) with the anti-human monoclonal antibody CD11b-PE (IM2581U, Beckman Coulter, Brea, CA, USA), CD14-FITC (555397 ...
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bioRxiv - Immunology 2020Quote: ... the cells were washed twice in cold phosphate buffer (PBS) before suspended in cold PBS and sorted on Moflo Astrios (Beckman Coulter). Sorted cells with different scattering intensity were stained to identify the percentages of CD3+ CD4+ T/CD3+ CD8+ T ...
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bioRxiv - Synthetic Biology 2020Quote: ... Cells were then filtered through a 40 μm cell strainer and analyzed by flow cytometry (MACSQuant VYB, Miltenyi or CytoFLEX, Beckman Coulter). We used EasyFlow ...
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bioRxiv - Immunology 2021Quote: ... To sort specific subsets of C-LP mononuclear phagocytes cells were gated as above and sorted by MoFlo Astrios Sorter (Beckman Coulter).
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bioRxiv - Immunology 2019Quote: ... Cells were sorted for high expression of receptor ± introduced adaptor or CD8 co-receptor by fluorescence-activated cell sorting (FACS) (MoFlo Astrios, Beckman Coulter).
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bioRxiv - Immunology 2019Quote: ... CHO ligand anchor HLA-A*02 cells were sorted for high expression of ligand anchor and HLA-A*02 using FACS (MoFlo Astrios, Beckman Coulter).
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bioRxiv - Neuroscience 2019Quote: ... Catalog L3224A) and lack of EtD-1 homodimer staining (L3224B) were selected and sorted by a MoFlo Astrios cell sorter (Beckman Coulter) into 500 µL filled into dissecting medium.
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bioRxiv - Biochemistry 2019Quote: ... Haemoglobin and red cell indices (MCV, MCH and MCHC) were determined using an automated haematology analyser (Beckman Coulter, Durban, South Africa). CD4-cell counts were assessed using BD FACS Count (BD Biosciences ...
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bioRxiv - Genetics 2020Quote: ... Timepoints were measured every 2-3 days by flow cytometry analysis of >7,000 cells (either a BD Accuri C6 or Beckman Coulter CytoFLEX). Data was analyzed using Cytoflow (https://github.com/bpteague/cytoflow ...
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bioRxiv - Biochemistry 2021Quote: ... to remove intact cells and the supernatant was centrifuged again at 25,000 RCF for 1 hour (Beckman Coulter JA-20 rotor). The pellet was resuspended in lysis buffer and sodium cholate was added to a final concentration of 1% and stirred at 4°C for 1 hour ...
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bioRxiv - Immunology 2021Quote: Infectivity and neutralization titers were determined using ACE2-expressing HEK293 target cells (Integral Molecular) in a semi-automated assay format using robotic liquid handling (Biomek NXp Beckman Coulter). Test sera were diluted 1:40 in growth medium and serially diluted ...
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bioRxiv - Cell Biology 2021Quote: ... BM analyses were terminal analyses at the time of tissue harvest and BM cellularity was determined using a ViCell automated cell counter (Beckman-Coulter).
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bioRxiv - Immunology 2020Quote: ... B- and NK cells within the lymphocyte population was performed by a whole blood staining lyse-no-wash protocol (Optilyse B, Beckman-Coulter) using six colour flow cytometry with fluorochrome-conjugated antibodies as listed in Supplementary Table 7 top ...
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bioRxiv - Immunology 2021Quote: ... cells were transferred to a 96 wells V-bottom plate and stained in PBS with ViaKrome 808 viability dye (Beckman Coulter). Next ...
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bioRxiv - Immunology 2020Quote: ... during 30min and then transferred into a DuraClone IF T Helper cell tube (CD3, CD4, IL-4, IL-17A, IFNγ) (Beckman Coulter), using the IntraPrep fixation permeabilization kit (Beckman Coulter) ...
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bioRxiv - Immunology 2020Quote: Intracellular cytokine production by T cells was analyzed after stimulating PBMC or whole blood (fresh or frozen) 4h at 37°C in DurActive1 tubes (Beckman Coulter), which contain PMA ...
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bioRxiv - Immunology 2021Quote: ... Insoluble proteins and cell debris were removed by centrifugation (13,000 rpm, 30 minutes at 5°C, JA-30.50Ti rotor, Beckman, Palo Alto, CA). The soluble CuMVTT CP protein in clarified lysate was pelleted using saturated ammonium sulfate (1:1 ...
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bioRxiv - Cell Biology 2021Quote: ... the cell lysate after removal of cell debris at 850 g was first pelleted with an Optima MAX-XP Ultracentrifuge (with TLA-100 rotor, Beckman Coulter) at 35,000 rpm (47,265 g ...
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bioRxiv - Bioengineering 2021Quote: ... cells were resuspended in 100 µL PBSB and data were acquired on a CytoFLEX S flow cytometer (Beckman Coulter, Brea, CA) and analyzed by FlowJo software (FlowJo LLC ...
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bioRxiv - Immunology 2022Quote: ... and total RNA was isolated using Isospin cell and tissue RNA kit (Nippon Gene) or an RNAdvance v2 kit (Beckman Coulter) according to manufacturer instructions and quantified with an RNA HS Assay Kit (Thermo Fisher ...
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bioRxiv - Cancer Biology 2022Quote: ... Single cells were flow sorted into single wells of 96-well plates using the MoFlo XDP100 cell sorter for ATTO550 positivity (Beckman Coulter) (University of Colorado Anschutz Medical Campus Cancer Center Flow Cytometry Core) ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were subsequently incubated with 2.5×103 CFSE-RJ-EVs per cell for 4 hours and analyzed with flow cytometry (CyAn ADP, Beckman Coulter, US). Treatment groups were compared to unstained control as well as CFSE-RJ-EVs without inhibitor ...
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bioRxiv - Biochemistry 2022Quote: ... to remove intact cells and the supernatant was centrifuged again at 25,000 RCF for 1 hour (Beckman Coulter JA-20 rotor). The pellet was resuspended in lysis buffer and sodium cholate was added to a final concentration of 1% and stirred at 4°C for 1 hour ...
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bioRxiv - Immunology 2022Quote: ... cells were seeded into pre-coated wells with anti-TCR pan-γδ clone IMMU510 antibody (5ug/ml) (Beckman Coulter, Krefeld, Germany) and IL-2 (100IU/ml) ...
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bioRxiv - Immunology 2022Quote: ... Cells were pelleted at 400xg for 5 min and washed with PBS twice before flow cytometry (Beckman Coulter CytoFLEX Flow Cytometer). Analysis of phagocytic ability was undertaken using FCS Express.
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bioRxiv - Immunology 2022Quote: CD27+ IgM- B cells cross-binding to SARS-CoV-1 and SARS-CoV-2 RBD were sorted from SARS-CoV-2 vaccinated SARS convalescents with MoFlo Astrios EQ Cell Sorter (Beckman Coulter). Briefly ...
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bioRxiv - Synthetic Biology 2022Quote: ... in an optimized version of the Light Plate Apparatus (LPA).53 HEK293T cells were analyzed 24h after illumination using CytoFLEX S Flow Cytometer (Beckman Coulter) as described previously.53 For each sample ...
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bioRxiv - Molecular Biology 2023Quote: ... After incubation for 24 hours the cells were harvested and analyzed for fluorescence intensity with a Beckman Coulter Gallios (Beckman Coulter). The result was analyzed by Kaluza Analysis 2.1 (Beckman Coulter ...
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bioRxiv - Molecular Biology 2022Quote: ... Equal optical density units from cleared cell lysates were loaded onto the gradient and centrifuged in a SW40 rotor (Beckman Coulter) for 5 hr ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were harvested after 3 days and counted using a Z2 Coulter Particle Count and Size Analyzer (Beckman Coulter, Brea, CA).
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bioRxiv - Microbiology 2024Quote: ... Stained cells were then washed once with PBS and ROS levels were analyzed using a CytoFLEX S flow cytometer (Beckman Coulter) with lasers at wavelengths of 405 nm (violet) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The FITC fluorescence signals demonstrating the antibody-receptor complex on the cell surface were quantified by flow cytometry (CytoFLEX, Beckman Coulter). Relative expression levels of GPR1 mutants were represented according to the fluorescence signals.
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bioRxiv - Cancer Biology 2024Quote: Analysis of GALNT3 or fibronectin expression on human or mouse pancreatic cancer cells was performed using a flow cytometer (Gallios, Beckman Coulter) with appropriate antibodies ...
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bioRxiv - Cancer Biology 2024Quote: ... Conventional flow cytometry analysis was performed on 200 µL of whole blood cells using a lyse no wash protocol (Versalyse lysing solution, Beckman Coulter) or on 2 ×106 PBMC labeled and analyzed using a Fortessa cytometer (BD Biosciences ...
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bioRxiv - Neuroscience 2024Quote: ... Biological titers of the viral preparations were assessed by transducing HEK293T cells with serial dilutions and checking the percentage of green fluorescent protein (GFP)-positive cells by flow cytometry (Cytomics FC 500, Beckman Coulter). Hippocampal-cultured neurons were transduced at 7DIV with lentiviral vectors (1-2 transducing units/cell).
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bioRxiv - Genetics 2024Quote: ... Resulting cell nuclei were gated for G1 phase (as determined by Hoechst and propidium iodide staining) and sorted into wells of 96 wells plates containing freezing buffer on a MoFlo Astrios cell sorter (Beckman Coulter). 30 nuclei of each were deposited per well ...
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bioRxiv - Genetics 2024Quote: ... and EGFP fluorescence was measured by FACS on either a SA3800 Spectral Cell Analyzer (Sony Biotechnology) or a CytoFLEX S Flow Cytometer (Beckman Coulter). Typically ...
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bioRxiv - Microbiology 2024Quote: Intracellular HIV-1 p24 staining was performed on fixed U1 and ACH-2 cells with KC57-FITC (Beckman Coulter, Cat#6604665). After allowing at least 30 minutes for fixation ...
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bioRxiv - Immunology 2024Quote: Individual CD24hi or CD24low Vγ1.1+ γδ T cells were isolated from Id3+/+ and Id3-/- mice by flow cytometry and deposited in wells of glass slides (Beckman-Coulter). Vγ1.1 and Vδ6.3 subunits were then amplified by RT-PCR using nested primers in single cells ...
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bioRxiv - Cell Biology 2023Quote: ... 20 µL of the cell lysate in each well were transferred to a Proxi plate by a Beckman NX (Beckman Coulter). The plates were then promptly de-ionized (to prevent ESI spray instability ...
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bioRxiv - Synthetic Biology 2023Quote: EGFP transfected cells and HEK-293-loxP-GFP-RFP cells before and after PLP coincubation were measured with a CytoFLEX S cytometer (Beckman Coulter) using 488 nm laser with a 525/50 filter for measuring EGFP expression and a 561 nm laser with a 610/20 filter for measuring RFP ...
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bioRxiv - Cell Biology 2023Quote: ... Resulting single nuclei of G1 phase (as determined by Hoechst and PI staining) were sorted into single wells of 96 wellplates on a MoFlo Astrios cell sorter (Beckman Coulter) and sorted at −80 degrees until further processing ...
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bioRxiv - Molecular Biology 2023Quote: ... stained the cells by a Annexin V-FITC/PI Apoptosis Detection Kit (Vazyme, A211-01) and detected cells distribution using a Beckman Coulter Gallios (Beckman Coulter). The result was analyzed by Kaluza Analysis 2.1 (Beckman Coulter).
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bioRxiv - Microbiology 2023Quote: ... followed by flow cytometry analysis of CAp24 antigen expression on fixed and permeabilized cells labelled with KC57-fluorescein isothiocyanate (FITC) (Beckman Coulter).