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Citations for Beckman :
1451 - 1500 of 1681 citations for Urothelial Cell Culture Supplements since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... live cells that were either GFP+ or RFP+ using a CytoFLEX S flow cytometer with CytExpert v2.4 software (Beckman Coulter). Analysis was performed using FlowJo v10.7 software (BD Life Sciences) ...
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bioRxiv - Cancer Biology 2023Quote: ... 7,500 GFP+ and 7,500 RFP+ single live cells were sorted into each well of a 24 well culture plate containing low pH DMEM with 10% FBS using a MoFlo Astrios cell sorter with Summit v6.3 software (Beckman Coulter). Sorting was performed at 25psi using a 100um nozzle ...
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bioRxiv - Cell Biology 2023Quote: ... whole cells (per sample) were pelleted via centrifugation at 1,700 xg (JA 20 rotor, Beckman Coulter Inc, Brea, CA, USA) for 10 min at room temperature ...
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Mechanism of RanGTP priming H2A-H2B release from Kap114 in an atypical RanGTP•Kap114•H2A-H2B complexbioRxiv - Biochemistry 2023Quote: ... Cells were lysed with an Emulsiflex-C5 cell homogenizer (Avestin) and centrifuged in an Avanti J-26 XPI with a JA-25.50 rotor (Beckman Coulter) at 20,000 RCF for 1 h at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Ramos B cells were double-stained with Alexa 488-cojugated Nef and anti-CD19-PC-5.5 (J3-119, Beckman Coulter), and interacting infected cell-invaded B cell doublet images acquired with ImageStream X MKII (Amnis/Merck Millipore ...
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bioRxiv - Cancer Biology 2023Quote: Cell death was evaluated 48 hrs after ionizing radiation (10 Gy) by using a CytoFLEX S (Beckman Coulter, Milan, Italy) instrument ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were seeded into 6-well plates at a concentration of 4×105 cells per well and counted cumulatively with a Coulter counter (Beckman) every three days for two weeks ...
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bioRxiv - Immunology 2023Quote: Flow cytometry analysis was performed at the end of the 7 day T cell progenitor differentiation stage using a CytoFLEX LX cytometer (Beckman), and data were compensated using single-stained controls in CytExpert v2.3 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Washed cells were then resuspended in PBS/BSA for analysis by flow cytometry on a CytoFlex flow cytometer (Beckman Coulter). Intact cells were identified based on their forward scatter/side scatter profile and staining intensity was monitored in the APC channel (FL4) ...
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bioRxiv - Immunology 2023Quote: ... Then this clear cell lysate was loaded on top the gradient and ultracentrifugation was done for 5hrs at 133,000g on SW60Ti (Beckman Coulter) rotor to separate each gradient ...
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bioRxiv - Cancer Biology 2023Quote: ... migrated cells to the lower chambers were counted as reported elsewhere [58] with fluorescent microbeads (Flow-Count Fluorospheres, Beckman Coulter) by flow cytometry.
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bioRxiv - Bioengineering 2023Quote: ... The cell uptake of nanoparticles were analyzed by flow cytometry using a CytoFLEX™ flow cytometer (Beckman Coulter, California, USA). For confocal imaging ...
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bioRxiv - Cancer Biology 2023Quote: ... the numbers of GFP+ and DsRed+ cells were determined by flow cytometry on a CytoFlex S Flow Cytometer (Beckman Coulter). For gating strategy see Supplementary Figure 4 in Supplementary Information ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were harvested at 6000 rpm for 30 min in a JLA 9.1000 rotor (Beckman Coulter, Brea, California, USA), washed with 150 mM NaCl ...
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bioRxiv - Cell Biology 2024Quote: Cell populations were analyzed for fluorescent protein expression using a CytoFLEX S flow cytometer (Beckman Coulter, San Jose, CA, USA). Single cell suspensions were isolated from each experimental condition 3-5 days after transfection ...
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bioRxiv - Cell Biology 2024Quote: ... The cell lysate was clarified via centrifugation at 30,000 rpm for 30 min in a Type 70 Ti rotor (Beckman Coulter) at 4°C ...
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bioRxiv - Microbiology 2024Quote: Counts of bacterial cells in the fixed gastric cavity fluid were conducted using flow cytometry (CytoFLEX LX, Beckman Coulter, USA), with filtered MilliQ water as the sheath fluid and a flow rate of 25 μL min-1 ...
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bioRxiv - Systems Biology 2024Quote: ... The supernatant was split to multiple ultracentrifuge tubes in order to remove insoluble cell debris by ultracentrifugation in a TLA 100.1 rotor (Beckman-Coulter) at 35,000 g at 4°C for 15 minutes ...
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bioRxiv - Genetics 2024Quote: ... Nuclei were then sorted to remove debris and nuclear aggregates on a MoFlow Astrios cell sorter (Beckman-Coulter, Brea, CA). Approximately,150,000 nuclei per sample were sorted and stored on ice prior to sn-RNA seq 5’ library generation.
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bioRxiv - Developmental Biology 2020Quote: ... incubated with fluorophore-conjugated antibodies for 30min (see Supplementary Table 1) and FACS-sorted using MoFlo Legacy or Astrios cell sorters (Beckman Coulter). Cells were sequentially gated based on size and granularity (forward scatter ...
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bioRxiv - Cell Biology 2022Quote: ... Yeast cells were stained with 5ug\ml propidium iodide for 10 minutes and samples were examined in CytoFlex flow cytometer (Beckman Coulter) using PE channel ...
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bioRxiv - Neuroscience 2021Quote: The cell suspension was passed through a 50 μm celltrix filter (Sysmex-Partec # 04-0042-2317) immediately before FACsorting the GFP+ NSCs using a MoFlo XDP cell sorter (Beckman Coulter) in the NYU Cytometry core facility ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell suspension was supplemented with 500 μl of 0.1% w/v BSA/PBS for adjustment of the volume prior to the analysis and subjected to flowcytometric analysis using Cell Lab Quanta SC (Beckman Coulter).
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bioRxiv - Molecular Biology 2021Quote: ... pombe cells were pelleted down for 10 min at 5000 rpm at 4°C (Rotor: F10-6X500, FiberLite Beckman J2-MC). The pellet was washed with buffer 1 (HEPES- 20 mM ...
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bioRxiv - Cell Biology 2022Quote: ... The cDNA amplification from single cells was performed by PCR for 21 cycles and the amplified products were purified using AMPure XP paramagnetic beads (Beckman Coulter).
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Targeting MOG to skin macrophages prevents EAE in macaques through TGFβ-induced peripheral tolerancebioRxiv - Immunology 2019Quote: ... Full hematology with blood cell count (CBC) was performed at each time of bleeding using an HMX A/L (Beckman Coulter). Immunological investigations were performed with fresh whole blood or isolated peripheral blood mononuclear cells (PBMCs).
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bioRxiv - Genetics 2019Quote: The PCR plates containing 25μl of cells per well were thawed and a modified protocol of the Agencourt GenFind V2 (Beckman Coulter, A41499) was utilized to isolate genomic DNA ...
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bioRxiv - Genomics 2020Quote: ... Stained cells were then washed with MACS buffer and the CD45+ Lin-CD38-CD34+ CD45RA-CD90+ fraction (HSCs) was FACS sorted using a MoFlo Astrios Cell Sorter (Beckman Coulter). Single-cell ATAC-seq data was then generated for the sorted HSCs using the dscATAC-seq Whole Cell protocol as previously described14.
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bioRxiv - Evolutionary Biology 2021Quote: ... The equation was established by combining OD measurements (λ = 600 nm) and cell counting in a flow cytometer (Beckman Coulter CytoFLEX) after staining with propidium iodide (PI).
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bioRxiv - Genomics 2021Quote: ... Cells were washed with 25 mm supplemented HBSS before depleting the sample of unnucleated cells using IOTest 3 Lysing solution (Beckman Coulter) following provider instructions ...
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bioRxiv - Neuroscience 2020Quote: ... This incubation step was followed by harvesting cells by centrifugation at 4000 rpm using JLA 8.1000 rotor (Beckman Coulter, Bear, CA) for 30 minutes at 5 °C ...
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bioRxiv - Cell Biology 2019Quote: The concentration of prey and THP-1 cells were measured prior to phagocytosis by flow cytometry (Accuri C6, BD or CytoFLEX, Beckman-Coulter), if needed ...
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bioRxiv - Biochemistry 2020Quote: ... to remove intact cells and the supernatant was centrifuged again at 25,000 g for 1 hour (Beckman Coulter JA- 20 rotor). The pellet was resuspended in lysis buffer and sodium cholate was added to a final concentration of 1% and stirred at 4°C for 1 hour ...
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bioRxiv - Microbiology 2020Quote: ... by using the single cell sorting option at a pressure of 60 psi on a MoFlo Astrios Flow Cytometer (Beckman Coulter) equipped with a 70 µm jet in air nozzle ...
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bioRxiv - Synthetic Biology 2021Quote: ... cells were diluted 100-fold in PBS to reach the final cell concentration of approximately 106 cfu/mL and measured on a CytoFLEX S (Beckman Coulter) using a FITC fluorescence channel with a 488 nm excitation laser and 525/40 nm emission band-pass filter with side scatter triggered events threshold set to 10,000 events ...
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bioRxiv - Systems Biology 2020Quote: ... Single clones of HEK293 cells expressing EPO or GFP were generated from the polyclonal batches by seeding single cells per well of 384-well plates by either limited dilution or FACS (Astrios, Beckman Coulter). In case of GFP-expressing cells ...
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bioRxiv - Microbiology 2020Quote: ... Whole blood staining was done with Alexa Fluor®647 labelled-VLP (2.5µg/ml) and cell subsets were discriminated using the following antibody panel: CD45 (Clone J33; Beckman Coulter, Brea, CA), CD11c (Clone BU15 ...
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bioRxiv - Microbiology 2021Quote: ... the cell suspensions were diluted in 1 mL and loaded onto a cytoflex-LX flow cytometer (Beckman Coulter, Brea, CA, USA) with a 375 nm laser ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell sorting was performed at the University of Colorado Cancer Center Flow Cytometry Shared Resource using an XDP-100 cell sorter (Beckman Coulter).
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bioRxiv - Cancer Biology 2020Quote: ... the cells in the T25 flask were harvested and counted by the Multisizer 4e coulter counter (Beckman Coulter, Brea, CA, USA).
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bioRxiv - Cell Biology 2021Quote: ... or Lin−Sca-1+c-Kit+CD150+CD48−CD34− (CD34−HSC) and Lin−Sca-1+c-Kit+ (LSK) cells were sorted on MoFlo Astrios EQ (Beckman Coulter). Quantification of CD71 ...
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bioRxiv - Molecular Biology 2022Quote: ... 2D10 cells were washed twice with 1 x phosphate buffered saline (PBS) and analyzed by flow cytometer (Epics Altra, BECKMAN COULTER) for the percentages of cells expressing GFP.
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bioRxiv - Microbiology 2022Quote: ... Washed pellets were resuspended in 2 mL 1x PBS and fluorescence was calculated using a LSR II cell analyzer (Beckman Coulter). Two biological replicates of 50,000 cell counts were analyzed with FlowJo ...
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bioRxiv - Microbiology 2022Quote: ... through a syringe needle (0.4-by 20 mm needles) and the cell wall was collected by ultracentrifugation at 90,000 × g for 45 min at 4°C (Beckman Coulter, Inc.). To the pellet ...
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bioRxiv - Biochemistry 2022Quote: ... NanoGlo luciferase assay substrate was prepared at a 1:100 dilution according to manufacturer’s protocol and 3 μl NanoGlo substrate was added to each well of media transfer plate and cell source plate with a BioRAPTR FRD (Beckman Coulter). Nanoluciferase luminescence for each plate was read on a ViewLux plate reader (PerkinElmer ...
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bioRxiv - Molecular Biology 2022Quote: Procyclic form cell lines for depletion of either TbPH1 or TbKifX2A were induced at a density of 2×106 cells/ml with 1 µg/ml doxycycline and growth monitored daily with a Z2 Coulter Counter Analyzer (Beckman Coulter). Cells were diluted back to the initial cell density every day ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were incubated for 2h at 20°C in an AN60-Ti rotor in the Optima-AUC analytical ultracentrifuge (Beckman Coulter) before data acquisition for 15h at 42,000 rpm ...
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Single cell analysis of the cellular heterogeneity and interactions in the injured mouse spinal cordbioRxiv - Neuroscience 2020Quote: ... were added to each sample well and cells were kept at 4°C until analysis on a Cytoflex Flow Cytometer (Beckman Coulter).
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bioRxiv - Microbiology 2021Quote: ... Growth rates were monitored for 96 h and cell densities were determined every 24 h using a Coulter Counter Z2 particle counter (Beckman Coulter). Procyclic forms (PCF ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were grown for 4-6 days and counted using an Incucyte (Essen Biosciences) or a Z2 Coulter Counter (Beckman Coulter). Small molecule dose-response curves were printed using an D300e Digital Dispenser (HP Specialty Printing Systems ...