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Citations for Beckman :
1551 - 1600 of 1672 citations for 7 Chloro 5 methyl 1H pyrrolo 2 3 c pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... and the tube was centrifuged for 2 hours at 190,000 x g in a swing-out rotor (SW40 Ti; Beckman Coulter ...
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bioRxiv - Microbiology 2021Quote: ... and either aliquoted or concentrated by ultracentrifugation using 20% (w/v) sucrose cushions for 2 hours at 25,000 rpm in an SW41 rotor (Beckman).
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bioRxiv - Microbiology 2022Quote: ... The pellet was resuspended in 2 ml (per fraction) of the supernatant and ultracentrifuged (Optima MAX-TL, Beckman Coulter) at 12,000 rpm (TLA55 rotor ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA clean-up was performed by incubating RNA solution in 2 volumes of AMPure XP Beads (Beckman Coulter, A63881) for 2 minutes at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... The filtrate was subjected to ultracentrifugation at 200,000 xg for 2 h (Optima L-100 XP ultracentrifuge; Beckman Coulter). Supernatants were discarded ...
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bioRxiv - Neuroscience 2023Quote: ... The tubes were centrifuged at 100,000 × g for 2 hours using a SW 55Ti swinging-bucket rotor (Beckman Coulter).
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bioRxiv - Biochemistry 2019Quote: ... centrifuged at 2000 × g for 10 min at room temperature and the plasma was separated and stored at −20°C until analysis on clinical chemistry analyzer (Beckman Coulter AU5800, Beckman Coulter Inc. Brea, CA; HUCFF-UFRJ), using kinetic colorimetric assays for α-amylase (Beckman OSR6006 ...
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bioRxiv - Immunology 2023Quote: ... UC-EVs were obtained by centrifuging 50 mL of conditioned media at 100,000 × g for 70 min at 4 °C (AH-629/36 rotor, Beckman Ultra-Clear Tubes with 36 ml capacity). Pellet was re-suspended in 250 µL of DPBS ...
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bioRxiv - Microbiology 2023Quote: ... briefly mixed and equilibrated for ∼30 min (Albumax equilibration was conducted in a 5% CO2 incubator to maintain pH 7.4) after which they were subjected to ultracentrifugation at 37°C using a sealed rotor (Beckman Rotor type 42.2 Ti; 223,000 x g) for 4.2 h to separate proteins ...
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bioRxiv - Neuroscience 2021Quote: ... Before FACsorting we added the 5 ul of the cellular death dye Draq7TM (Viability dye, Far-red DNA intercalating agent, Beckman Coulter, #B25595). The suspension was sorted on an Astrios II cell sorter (Beckam Coulter) ...
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bioRxiv - Molecular Biology 2019Quote: ... 4 ml of 10 % and 3.5 ml of 5 % iodixanol in a 17 ml Thinwall Polypropylene Tube (Beckman Coulter, Fullerton, California, USA). The DG was centrifuged 18 h at 100,000 g and 4 °C using SW 32.1 Ti rotor (Beckman Coulter ...
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bioRxiv - Microbiology 2019Quote: The 5-10 mL bioaerosol samples were concentrated by spinning at 3000 × g for 10 min (Allegra® X-15R, Beckman Coulter). The pellet was resuspended in 200 µl fresh 7H9 and stained overnight (12-16 h) ...
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bioRxiv - Neuroscience 2019Quote: ... iodixanol solution and overlaid with 7.0 mL of 25% (v/v) iodixanol and 350 µL of 5% (v/v) iodixanol in a polyalomar tube (Beckman, Miami, FL). All of the above iodixanol solutions were prepared in lipid flotation buffer (10 mM HEPES ...
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bioRxiv - Immunology 2021Quote: ... were loaded onto 5-25% (w/v) linear sucrose gradients and centrifuged in a Beckman Optima L-90K Ultracentrifuge (Beckman SW40 rotor) at 40,000 rpm for 3 h 15°C ...
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bioRxiv - Genomics 2021Quote: ... with 5 PCR reactions per column then pooled together and cleaned up a second time with 1.6X SPRI beads (Beckman Coulter, Brea, California). The CHEQ-seq vector containing a random 17 bp BC upstream of the synthetic intron was linearised via inverse PCR with primers “CHEQseq_lin_A_For” and “CHEQseq_lin_A_Rev” or “CHEQseq_lin_B_For” and “CHEQseq_lin_B_Rev” ...
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bioRxiv - Neuroscience 2022Quote: ... was loaded onto a sucrose gradient made up of 5 ml 1.2 M sucrose on the bottom and 5 ml of 0.8 M sucrose at the top in thin wall ultracentrifugation tubes (Beckman Coulter, Cat # 344059). The loaded gradient was ultracentrifuged at 69,150 x g for 1 h at 4 °C (SW 41 Ti Swinging-Bucket Rotor ...
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bioRxiv - Cancer Biology 2023Quote: ... Conditioned media were centrifuged at 1000 rpm for 5 min to remove cell debris and then either added to Ultra-Clear Quick-Seal ultracentrifuge tubes (Beckman Coulter 246.344322), 100 kDa molecular weight cut-off centrifugal filters (MWCO ...
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bioRxiv - Plant Biology 2023Quote: ... and 2 mL of 5% solution in a 14 x 89 mm (13.2 mL) thin walled ultra-clear centrifuge tubes (Beckman Coulter, Cat. # 344059). Approximately 250 µl of crude EV sample resuspended in 20 mM Tris-HCl pH 6.0 was slowly dispensed on top of the column and centrifuged at 100,000 x g for 17 h at 4 ⁰C (SW 41 Ti rotor ...
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bioRxiv - Microbiology 2024Quote: ... Liposomes and liposome-protein complexes were separated from unbound protein by ultracentrifugation of the reaction mixture in 5 mL Optiprep™ (Axis-Shield PoC AS) density gradients for 1 hour at 40,000 ×g (SW55Ti Beckman Coulter rotor). The liposome-protein mixture was adjusted to 34% Optiprep concentration in a volume of 300 μl ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were stained with 5 μg/ml PI (ST1569-10mg, Beyotime) and immediately analyzed using the CytoFLEX flow cytometer (Beckman Coulter, USA). Tumor cells were distinguished based on GFP positivity or CD45 negativity ...
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bioRxiv - Biochemistry 2020Quote: ... Protein expression was induced with 0.4 mM Isopropyl β-D-1-thiogalactopyranoside and cells were grown at 37 °C overnight before harvesting by centrifugation at 4000 rpm (Beckman Coulter Avanti JXN-26; JLA8.1 rotor) at 10 °C for 10 min.
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bioRxiv - Biochemistry 2019Quote: ... Protein expression was induced with 0.4 mM Isopropyl β-D-1-thiogalactopyranoside and cells were grown at 25 °C overnight before harvesting by centrifugation at 4000 rpm (Beckman Coulter Avanti JXN-26; JLA8.1 rotor) at 4 °C for 15 min.
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bioRxiv - Genomics 2020Quote: ... RAD library quality was inspected on a 2% agarose gel before undergoing a final AMPure XP (Beckman Coulter, Indianapolis, IN) purification and quantification on a Qubit 2.0 Fluorometer (ThermoFisher Scientific ...
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bioRxiv - Genomics 2020Quote: ... The crude nuclei were stained with 4,6-diamidino-2-phenylindole and loaded into a flow cytometer (Beckman Coulter MoFlo XDP). Nuclei were purified by flow sorting and washed in accordance with (70) ...
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bioRxiv - Microbiology 2019Quote: ... Sucrose gradient ultracentrifugation was performed for 2 hours and 45 minutes at 35,000 rpm in a SW 41 Ti rotor (Beckman-Coulter) at 4°C ...
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bioRxiv - Neuroscience 2019Quote: ... with 2 ml of 20% sucrose in dPBS cushion and ultracentrifugated at 20,000 RPM (Beckman Coulter SW 32 Ti rotor) at 4°C for 2 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... 100 ug/ml cycloheximide) and macromolecular complexes were separated by velocity gradient ultracentrifugation (SW41Ti – Beckman, 39,000 rpm, 2 hr, 4oC). Fractions were collected using Biocomp Gradient Station and pooled to obtain separate monosomal and polysomal fractions ...
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bioRxiv - Cell Biology 2021Quote: ... Collected FT were then subjected to ultracentrifugation for 2 hours at 200 000g using the TLA 45 rotor (Beckman Coulter) and resuspended in 20μL of Laemmli 1X (BioRad) ...
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bioRxiv - Biochemistry 2021Quote: ... Equal parts of 30% and 10% iodixanol gradient solutions were layered on the mitochondrial fraction and then samples were centrifuged at 50,000 x g for 2 h (Beckman SW41TI). The mitochondrial layer was collected ...
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bioRxiv - Cell Biology 2021Quote: Microtubules were sedimented onto poly-L-lysine coated coverslips by centrifuging through 20% glycerol BRB80 cushion at 4700 rpm for 2 h in S6096 rotor (Beckman). Coverslips were subsequently fixed with 20°C methanol ...
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bioRxiv - Synthetic Biology 2021Quote: ... The collected supernatants were centrifuged at 300,000 x g for 2 hr with Optima MAX-XP ultracentrifuge with SW 55 Ti Swinging-Bucket Rotor (Beckman Coulter). The pellets consisting of the cell membrane fractions were resuspended into 10 mL of the same glycan containing media and incubated in the anaerobic chamber at 37°C for 16 hr ...
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bioRxiv - Microbiology 2021Quote: ... Pool 1 and pool 2 amplicons were combined and purified with a 1:1 ratio of AMPureXP beads (Beckman Coulter) and eluted in 30 μL of sterile water ...
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bioRxiv - Cell Biology 2020Quote: ... The supernatant was then overlaid onto a 2 ml 60% iodixanol cushion and centrifuged at 100,000 × g (SW32 rotor, Beckman Coulter) for 18h ...
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bioRxiv - Molecular Biology 2022Quote: ... Single-cell clones were then assessed for binding to His-tagged SARS-CoV-2 Spike using a Cytoflex S (Beckman) cell analyser ...
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bioRxiv - Developmental Biology 2020Quote: ... Virus-containing supernatant was collected for two consecutive days and concentrated by ultracentrifugation for 2 hours at 19,400 rpm using an Optima L-80 XP Ultracentrifuge (Beckman Coulter).
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bioRxiv - Microbiology 2021Quote: Total RNA was isolated from Vero E6 and Caco-2 cells 48 hours after infection using RNAdvance Viral Kit (Beckman) using a KingFisher and RNeasy Mini Kit (Qiagen) ...
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bioRxiv - Cancer Biology 2020Quote: ... samples were suspended in FACS buffer (PBS, 20 mM EDTA pH 8.0, 2% FCS) and acquired using a CyAn ADP9 flow cytometer (Beckman Coulter), FACS LSRII Fortessa or FACSymphony (both BD Biosciences) ...
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bioRxiv - Microbiology 2021Quote: ... pspAX.2 and oligonucleotides were incubated for 48 h and GFP expression level was analyzed by CytoFLEX flow cytometer (Beckman).
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bioRxiv - Neuroscience 2022Quote: ... new medium was added for another round of production and supernatant was filtered (0.45 μm filter) and centrifuged for 2 hours at 19400 rpm (SW28 Beckman rotor). The pellets were suspended in phosphate buffer saline (PBS) ...
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bioRxiv - Synthetic Biology 2021Quote: Reactions were assembled in a final volume of 2 μL using a Labcyte Echo® 550 (Beckman Coulter, Brea, CA) via a two-step strategy ...
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bioRxiv - Neuroscience 2021Quote: ... The resulting homogenate was mixed with 6 mL sucrose (2 mol/L) and 2.5 mL CaCl2 (0.1 mmol/L) in an ultra-clear centrifuge tube (#344059, Beckman Coulter, USA). Then ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were ultracentrifugated at 100,000g for 2 hours using OPTIMA MAX XP ULTRACENTRIFUGE with MLA-130 fixed-angle rotor (Beckman-Coulter) and pellets were resuspended in 0.22 μm-filtered PBS ...
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bioRxiv - Microbiology 2021Quote: ... 2 ml of each sucrose concentration was layered from 70% to 25% in open-top ultracentrifuge tubes (Beckman Coulter, 344059) immediately before use ...
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bioRxiv - Biochemistry 2021Quote: ... The depleted fraction was collected by gentle centrifugation (100 rcf for 2 min) into a clean 96 well plate (Beckman Coulter ...
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bioRxiv - Bioengineering 2022Quote: ... the conditioned media was subjected to an additional centrifugation step of 118,000 × g for 2 h in a Type 70 Ti ultracentrifuge rotor (Beckman Coulter). The resulting EV pellet was resuspended in 1X PBS and placed into a Nanosep 300 kDa MWCO spin column (Pall ...
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bioRxiv - Microbiology 2022Quote: ... Pool 1 and pool 2 amplicons were combined and purified with a 1:1 ratio of AMPureXP beads (Beckman Coulter) and eluted in 30 µL of RNAase free water ...
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bioRxiv - Neuroscience 2023Quote: ... The top 11 mL and the bottom 2 mL of the gradient were transferred to separate 60 mL 45Ti ultracentrifuge tubes (Beckman), topped up with PBS and centrifuged at 100,000 x g for 70 min at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... DNase-treated RNA was subsequently purified using RNase-free Agencourt AMPure XP beads (Beckman Coulter; 2:1 volume of beads:sample).
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bioRxiv - Plant Biology 2023Quote: ... and water to a final volume of either 2 μL for the acoustic liquid handling robot (Labcyte Echo 550, Beckman) or 5 μL for manual handling ...
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bioRxiv - Biophysics 2023Quote: ... the supernatant of cell lysates was centrifuged to isolate the total membrane fraction at 24,000 rpm for 2 h in the 45Ti rotor using ultracentrifuge (Beckman-Coulter). Membrane resuspension in 50 mM Tris-HCl buffer (pH 8.0 ...