Labshake search
Citations for Beckman :
1351 - 1400 of 1672 citations for 7 Chloro 5 methyl 1H pyrrolo 2 3 c pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Evolutionary Biology 2020Quote: ... Mitochondria were purified using a series of differential centrifugations: 20 min at 1500 g to pellet residual chloroplasts (8 °C, 3500 RPM, Beckman JA-12), 20 min at 20 000 g to pellet mitochondria (8 °C ...
-
bioRxiv - Microbiology 2020Quote: ... All bacteria were harvested by centrifugation at 4000 rpm and 4°C in a JS 4.0 swinging bucket rotor (Beckman Coulter, Brea California) and immediately processed as described below.
-
bioRxiv - Immunology 2020Quote: ... Plate was then incubated 1 h à 37 °C with 1 μg per well of alkaline phosphatase-coupled anti-Igκ or anti-IgM (Beckman Coulter) in PBS ...
-
bioRxiv - Microbiology 2021Quote: ... density gradient (35 to 0% in PBS) and ultracentrifuged at 200,000 × g for at least 12 hours at 4 °C (Beckman Optima XL-100K). The corresponding clear yellow band was collected ...
-
bioRxiv - Cell Biology 2022Quote: ... The resulting supernatant was centrifuged at 10,000 x g for 30 minutes to remove any remaining debris and then ultra-centrifuged at 118,000 x g for 70 minutes at 4°C with a fixed-angle rotor (Ti-70, Beckman Coulter, Brea, CA). The pellet was washed with sterile 1X PBS and subjected to another cycle of ultracentrifugation at 118,000 x g for 70 minutes at 4°C ...
-
bioRxiv - Bioengineering 2022Quote: ... The supernatant was transferred into polycarbonate tubes and centrifuged at 20,000 ×g for 45 min at 4°C to pellet down m/lEVs using an Optima XE-90 ultracentrifuge equipped with a 50.2 Ti rotor (Beckman Coulter, Indianapolis, IN). Next ...
-
bioRxiv - Cancer Biology 2022Quote: ... The supernatants were further centrifuged at 10,000 × g for 30 min at 4°C to remove remaining cell debris and at 100,000 × g (Type 90 Ti Rotor, Beckman, Brea, CA, USA) overnight at 4°C to remove exosomes ...
-
bioRxiv - Biochemistry 2022Quote: ... Triton-X was added to the lysate to a final concentration of 0.1% and clarified by spinning at 15,000 RCF at 4°C for 45 minutes (Beckman Coulter JA-20 rotor). The supernatant was loaded onto a 5 mL HisTrap™ FF crude column (GE Healthcare ...
-
bioRxiv - Molecular Biology 2023Quote: ... The cell lysate was centrifuged at 4 °C at 35000 rpm for 1 hour with Beckman Optima™ L-80XP Ultracentrifuge equipped with Ti45 rotor (Beckman, USA). The pellet containing membranes and cell debris was discarded ...
-
Isolation and characterization of a novel phage of Vibrio parahaemolyticus with biocontrol potentialbioRxiv - Microbiology 2022Quote: ... for purification and centrifuged at 200,000 × g at 4°C for 24 h using an Optima L-100 XP ultracentrifuge (Beckman Coulter, CA, USA). The purified phage particles were dialyzed through 30-kDa superfilters (Millipore ...
-
bioRxiv - Neuroscience 2023Quote: ... re-suspend in the homogenization buffer and centrifuged at 100,000 x g for 40 min at 4°C (Beckman SWTi41 swing rotor). The pellet containing the growth cone were re-suspended in 50 μl of the homogenization buffer ...
-
bioRxiv - Neuroscience 2022Quote: ... The loaded gradient was ultracentrifuged at 69,150 x g for 1 h at 4 °C (SW 41 Ti Swinging-Bucket Rotor, Beckman Coulter, Cat. #331362) and the synaptosome fraction was harvested from the interface between the two sucrose layers ...
-
bioRxiv - Biochemistry 2022Quote: ... The supernatant was again centrifuged at 100000 g for 45 min at 4°C (Beckman Optima™ MAX-XP Rotor MLS-50). After filtering the supernatant through a 0.22 μm syringe filter ...
-
bioRxiv - Cell Biology 2022Quote: ... One milliliter of concentrated conditioned medium was pipetted on top of the gradient and samples were centrifuged for 18 h at 100,000 ×g and 4 °C using a SW 32.1 Ti rotor (Beckman Coulter, Fullerton, California, USA). Fractions of 1 mL were collected from the top and EV-rich fractions 9 and 10 (corresponding to a density of 1.10-1.12 g/mL ...
-
bioRxiv - Microbiology 2023Quote: ... The lysate was clarified by centrifugation at 5500 rpm for 20 min at 4°C (AllegraTM 21R Centrifuge, Beckman Coulter, CA, USA). The sample was then loaded into a HisTrap HP 5 mL column (Cytiva ...
-
bioRxiv - Neuroscience 2023Quote: ... then spun at 100,000 x g for 30 min at 4°C in an ultracentrifuge (Beckman OPTIMA TLX with TLA-100.3 rotor). The supernatant was saved and the pellet went through a series of similar extractions using High-Salt Triton Buffer (High-Salt Buffer ...
-
bioRxiv - Neuroscience 2023Quote: ... The supernatants were then combined and centrifuged at 17,000g for 10min at 4°C in a Beckman SW41-Ti rotor (Beckman Coulter, Brea, CA). The supernatant was saved as the cytosolic (CYT ...
-
bioRxiv - Biochemistry 2023Quote: ... Triton-X was added to the lysate to a final concentration of 0.1% and clarified by spinning at 15,000 RCF at 4°C for 45 minutes (Beckman Coulter JA-20 rotor). The supernatant was loaded onto a 5 mL HisTrap™ FF crude column (GE Healthcare ...
-
bioRxiv - Bioengineering 2023Quote: ... The supernatant was then transferred into polycarbonate tubes and centrifuged at 20,000 ×g for 45 min at 4°C to pellet down m/lEVs using an Optima XE-90 ultracentrifuge fitted with a 50.2 Ti rotor (Beckman Coulter, Indianapolis, IN). Following that ...
-
bioRxiv - Developmental Biology 2023Quote: ... for 30 min at 4°C in the dark followed by flow cytometry analysis on a CytoFlex 26 (Beckman Coulter, Indianapolis, IN) in PE-A and PerCP-A channels ...
-
bioRxiv - Biochemistry 2023Quote: ... by centrifugation at 80,000 × g for 30 min at 4°C using a TLS-55 rotor (Beckman Coulter Inc., Miami, FL, USA). Crista membranes were identified and removed based on fluorescence under a UV lamp ...
-
bioRxiv - Cell Biology 2023Quote: ... Following centrifugation at 131,300 × g for 24 hr at 4 °C using a MLS-50 Swinging-Bucket rotor (Beckman Coulter, Brea, CA) to sediment protein complexes ...
-
bioRxiv - Cancer Biology 2024Quote: ... The plates were then centrifuged at 2000 rpm (931 x g) for 2h at 30°C (Allegra® X-15R Centrifuge (Beckman Coulter), rotor SX4750A) ...
-
bioRxiv - Microbiology 2024Quote: ... Saliva was collected by expectoration into tubes on ice followed by centrifugation for 10 minutes at 4°C at 4000 x g (Beckman SX4750 rotor) to remove large debris and bacterial aggregates ...
-
bioRxiv - Biophysics 2024Quote: ... The gradients were then centrifuged at 187,000 × g for 4 h at 4°C in a SW 41 Ti rotor (Beckman Coulter, Brea, CA). Bands corresponding to purified virus particles (∼1.36 g/mL ...
-
bioRxiv - Cell Biology 2024Quote: ... The collected monosomes were then 1:1 diluted with PEB and centrifuged at 60,000 rpm at 4°C overnight on an SW65 Ti ultracentrifuge rotor (Beckman Coulter, California, USA). The pellets were resuspended in 250µl PEB with 50 mM NH4Cl ...
-
bioRxiv - Cell Biology 2024Quote: ... All centrifugation steps were done at 4°C and the last two steps were performed using Optima XE-90 ultracentrifuge (Beckman Coulter, A94471) with rotor SW32Ti (Beckman Coulter ...
-
bioRxiv - Biochemistry 2023Quote: ... Cultures were then recovered by centrifugation (4,000 x g, 4 °C, 40 min in a Beckman Avanti JXN-26 (rotor: JLA 8.1)) and pellets resuspended in approximatively 30 mL of lysis buffer (20 mM HEPES ...
-
bioRxiv - Molecular Biology 2024Quote: ... ADEV isolation was performed by ultracentrifugation at 100,000 × g for 14 h at 4 °C (swing bucket rotor on minimum acceleration/break setting, SW 32Ti Beckman Coulter, Brea, CA), and ADEVs were resuspended in 200μL PBS and stored at −20°C until used16,41 ...
-
An anti-amyloidogenic treatment to specifically block the consolidation of traumatic events in mousebioRxiv - Animal Behavior and Cognition 2020Quote: ... the rest of the volume was centrifuged for 5 minutes at 4,000 rpm (Microfugue 18 centrifuge, Beckman Coulter) to obtain the cell debris fraction (P1 ...
-
bioRxiv - Biochemistry 2020Quote: ... After 20 minutes the cells were collected by centrifugation (8,000 rpm, 5 minutes, JLA-16.25 Beckman Coulter rotor), dissolved in 0.5 mL of HEPES:Polymix buffer (5 mM Mg(OAc)2 ...
-
bioRxiv - Genetics 2021Quote: ... Reactions were then pooled in a 5 ml tube and purified using a 0.7x SPRIselect (Beckman Coulter B23318) cleanup and eluted in 450μL of TE ...
-
bioRxiv - Molecular Biology 2023Quote: ... Dialyzed nucleosomes were purified in a 5-30% w/v sucrose gradient with a SW41 Ti (Beckman Coulter) rotor in an Optima L-90K ultracentrifuge (Beckman Coulter ...
-
bioRxiv - Cell Biology 2022Quote: ... The product from PCR-2 was purified using 1x volume of AMPure XP beads (Beckman Coulter) and eluted in 15 μL of IDTE buffer (IDT DNA) ...
-
bioRxiv - Bioengineering 2021Quote: ... The supernatant was then ultracentrifuged for 2 hours using Optima™ L-80XP Ultracentrifuge (Beckman Coulter) at 100,000 x g at 4°C ...
-
bioRxiv - Molecular Biology 2020Quote: ... The solution was centrifuged at 36,000 rpm for 2 h in a SW41Ti rotor (Beckman Coulter) at 4°C ...
-
bioRxiv - Genetics 2021Quote: ... We purified the final libraries with 2 rounds of 0.8X volume of SPRISelect (Beckman Coulter, B23318).
-
bioRxiv - Immunology 2020Quote: ... were incubated with 1-2 million 1D8 cells following standard FACS protocol and analyzed by Beckman.
-
bioRxiv - Microbiology 2021Quote: ... and centrifuged for 2 h at 64,000 g in A27-8×50 mL rotors (Beckman Coulter) to sediment virus particles ...
-
Dynamic states of eIF6 and SDS variants modulate interactions with uL14 of the 60S ribosomal subunitbioRxiv - Biochemistry 2022Quote: ... Gradients were centrifuged at 35,000 rpm for 2 hrs 40 min using a SW41Ti rotor (Beckman). Gradients were fractionated using a Brandel (UV ...
-
bioRxiv - Microbiology 2023Quote: ... Three 1:2 serial dilutions were done suing a Biomek i7 liquid handler (Beckman Coulter, USA), in order to obtain the four working concentrations ...
-
bioRxiv - Cell Biology 2022Quote: ... Equal volume (2 ml i.e 1600 µg) of cell extracts were loaded in ultracentrifuge tubes (Beckman). Samples were ultracentrifuged at 100,000 g for 30 minutes at 4°C in a Optima XPN-100 ultracentrifuge (Beckman) ...
-
bioRxiv - Bioengineering 2023Quote: ... in 2%BSA and 2mM EDTA and sorted using FACS sorter (Beckman Coulter, MoFlo Astrios Zellsortierer). For gating ...
-
bioRxiv - Immunology 2023Quote: Cells were washed with 2% FACs and data was acquired using CytoFLEX S (Beckman Coulter, USA) and analyzed via FlowJo v10 software (Oregon ...
-
bioRxiv - Microbiology 2023Quote: ... resuspended in 2% BSB-PFA and analysed by Flow Cytometry on BD Celesta instrument (Beckman Coulter) using FlowJoTM software ...
-
bioRxiv - Biophysics 2022Quote: ... The fractions containing monomeric peptide were pooled and centrifuged at 386000 g for 3 h in an Optima TLX ultracentrifuge equipped with a TLA 120.1 rotor (Beckman Coulter, Atlanta, GA, USA). Orthophosphoric acid or sodium phosphate were added to a final concentration of 20 mM to adjust the pH to 2.8 or 7.4 ...
-
bioRxiv - Biochemistry 2021Quote: The absorbance spectra of recombinant DosS and DosT (3 µM) were determined at room temperature using quartz cuvettes with rubber stoppers in a DU800 spectrophotometer (Beckman Coulter, Fullerton, CA). The reduction of recombinant DosS was achieved via addition of sodium dithionite (DTH ...
-
bioRxiv - Microbiology 2022Quote: ... induced by doxycycline (DOX) at 3 μg/ml and sorted for high GFP-expressing cells by MoFLO Astrios EQs (Beckman Coulter Life Sciences) using 488 nm lasers ...
-
bioRxiv - Microbiology 2024Quote: ... reinhardtii cell density and size were determined using a hemocytometer and ImageJ or a Beckman Coulter Multisizer 3 with a 50 μm orifice (Beckman Coulter, CA, USA). Bacterial cell density was determined by counting colony forming units (CFU ...
-
bioRxiv - Bioengineering 2023Quote: ... the supernatant was then taken and loaded into a 10% to 45% sucrose gradient solution and centrifuged at 360000 rpm for 3 h (Beckman Optima XE-100). 18 fractions from each sample were collected using a piston gradient fractionator (Biocomp ...