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Citations for Beckman :
1051 - 1100 of 1547 citations for Mouse Anti Hepatitis C Virus NS3 Antibody 1828 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2024Quote: ... and pelleted each time by ultracentrifugation at 100,000 x g for 70 mins at 4 °C in polyallomer tubes (Beckman Coulter) in a SW40 rotor (Beckman Coulter) ...
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bioRxiv - Immunology 2022Quote: ... anti-human TCR Vβ1 (Beckman Coulter), anti-human CD3 and CD8 antibodies (BD Bioscience).
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Targeting MOG to skin macrophages prevents EAE in macaques through TGFβ-induced peripheral tolerancebioRxiv - Immunology 2019Quote: ... anti-CD28 (clone 28.2, Beckman Coulter); anti-CD69 (FN50 ...
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bioRxiv - Immunology 2019Quote: ... anti-h2B4 (C1.7, Beckman Coulter, IM1607), anti-hCD16 (3G8 ...
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bioRxiv - Immunology 2021Quote: ... APC-AF750 anti-CD38 (Beckman Coulter).Intracellular staining for transcription factors was carried out after cell fixation and permeabilization using the Transcription Factor Buffer Set (BD Biosciences ...
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bioRxiv - Cell Biology 2021Quote: ... anti-PAR1 WEDE (Beckman Coulter, #IM2584), and anti-Cav-1 (CST ...
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bioRxiv - Neuroscience 2022Quote: ... anti-CD45 (A07785 / Beckman Coulter / J33), anti-CD90 (IM1839U / Beckman Coulter / F15-42-1-5) ...
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bioRxiv - Neuroscience 2022Quote: ... APC-tagged anti-CD41 (Beckman Coulter).
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bioRxiv - Neuroscience 2022Quote: ... anti-CD34 (IM2709U / Beckman Coulter / 581), anti-CD45 (A07785 / Beckman Coulter / J33) ...
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bioRxiv - Immunology 2024Quote: ... anti-CD69-PE (#IM1943U, Beckman Coulter) and CD56- PC7 (#A21692 ...
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bioRxiv - Immunology 2024Quote: ... Anti-CD107a-PB (#B13978, Beckman Coulter) antibody and monensin (Golgi StopTM ...
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bioRxiv - Immunology 2024Quote: ... anti-CD45-KrO (#B36294, Beckman Coulter), anti-CD3-APC (#IM2467 ...
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bioRxiv - Immunology 2024Quote: ... anti-CD69-PE (#IM1943U, Beckman Coulter), CD56-PC7 (#A21692 ...
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bioRxiv - Immunology 2024Quote: ... anti-CD3-APC (#IM2467, Beckman Coulter), anti-CD8-APC-AF700 (#B49181 ...
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bioRxiv - Immunology 2024Quote: ... anti-CD107a-PB (#B13978, Beckman Coulter), anti-CD45-KrO (#B36294 ...
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bioRxiv - Immunology 2024Quote: ... anti-CD3-APC (#IM2467, Beckman Coulter), anti-CD8-APC- AF700 (#B49181 ...
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bioRxiv - Biophysics 2023Quote: ... anti-CD3-PE/Cy7 (Beckman Coulter) and anti-CD8-PerCPCy5.5 (BD ...
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bioRxiv - Microbiology 2020Quote: ... were added into cells (1×106 cells/sample in 100 μL) and incubated for 60 min at 2-6°C and analyzed using flow cytometry (CytoFLEX, Beckman Coulter).
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bioRxiv - Microbiology 2020Quote: ... Cultures of 1 ml in PYNal were incubated at 30°C (250 rpm) to an optical density of 0.2 at 620 nm (OD620; Beckman DU650 spectrophotometer) in 96-deep-well plates ...
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bioRxiv - Plant Biology 2021Quote: ... the samples were centrifuged at 35 000 RPM for 18 hours at 4°C in a 70Ti rotor and L8-M ultracentrifuge (Beckman Coulter). The ribosome pellets were then gently washed with RNase-free water and resuspended in 300 μl of resuspension buffer (100 mM Tris·HCl (pH 8.0) ...
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bioRxiv - Cell Biology 2019Quote: ... by ultracentrifugation at 150,000 × g for 12 h at 4°C using an Optima L-100XP centrifuge equipped with a SW55Ti rotor (Beckman Coulter). Eleven fractions were collected from the top of the sucrose gradient.
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bioRxiv - Cell Biology 2020Quote: ... Samples were pelleted at 280’000 x g for 10 min at 25 °C in an Optima XP centrifuge with a TLA-100 rotor (Beckman-Coulter). Supernatant and pellet fractions were separated on a 10 % SDS-PAGE gel and proteins were visualized by Coomassie Blue staining ...
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bioRxiv - Evolutionary Biology 2021Quote: ... using a Beckman Coulter TLA100 rotor at 70,000 rpm at 4 C for 2 hours in a 13 mm x 51 mm polycarbonate ultracentrifuge tube (Beckman Coulter 349622). The sucrose solution was removed from the tube ...
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bioRxiv - Neuroscience 2021Quote: ... 4 × 5-s bursts) and subsequently centrifuged at 48,000g at 4°C (Beckman Avanti J-251 Ultracentrifuge; Beckman Coulter, Fullerton, CA) for 30 min ...
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bioRxiv - Molecular Biology 2021Quote: ... The gradient was ultracentrifuged at 55,000 rpm for 55 min at 4 °C with max acceleration and slow deceleration using an Optima LE-80K Ultracentrifuge and SW55Ti rotor (Beckman Coulter). The sucrose gradient was fractionated according to the number of associated ribosomes as determined by the profile of the absorbance at 254 nm using a Density Gradient Fractionation System (Brandel ...
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bioRxiv - Neuroscience 2021Quote: ... This was incubated for 1 hour at room temperature while rotating before centrifugation at 100,000 x g for 90 minutes at 4°C using the Optima™ MAX-XP Ultracentrifuge (Beckman Coulter). The supernatant was then collected (sarkosyl-soluble fraction) ...
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bioRxiv - Molecular Biology 2021Quote: ... pombe cells were pelleted down for 10 min at 5000 rpm at 4°C (Rotor: F10-6X500, FiberLite Beckman J2-MC). The pellet was washed with buffer 1 (HEPES- 20 mM ...
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bioRxiv - Bioengineering 2022Quote: ... The 60-70 mL were then divided equally into two 50-mL centrifuge tubes and spun down outside the anaerobic chamber at 4 °C and 3500 rpm for 10 min using an Allegra 64R ultracentrifuge (Beckman Coulter). Next ...
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bioRxiv - Bioengineering 2022Quote: ... Each 250 mL culture was divided equally into six 50-mL centrifuge tubes and spun down at 4 °C and 3500 rpm for 10 min using an Allegra 64R centrifuge (Beckman Coulter). The supernatant was discarded and 6 mL of ice-cold 10% glycerol was used to wash and combine the six cell pellets into one suspension ...
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bioRxiv - Genomics 2020Quote: ... Lentiviral supernatant was collected at 48 and 72hrs post-transfection and subsequently concentrated by ultracentrifugation (24,000 rpm, 4°C, 2hrs) (Beckman Coulter SW32).
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bioRxiv - Cancer Biology 2019Quote: ... betulina (LB) after oven dried at 50°C temperature for 48 h were ground by mixture grinder (Beckman food mixer, India). This smashed biomass (100 g ...
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bioRxiv - Molecular Biology 2019Quote: ... The cleared supernatant was layered over 15-40% sucrose gradients in disassociation buffer and spun 28000 rpm for 16 hours at 4°C in a SW-32 rotor (Beckman-Coulter). Gradients were fractionated with an ISCO fractionation system and 30S + 50S peaks were combined ...
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bioRxiv - Genetics 2020Quote: ... The homogenates were incubated on ice for 15–20 min and then centrifuged at 3000xg for 10 min at 4°C in a GPR Data Settop centrifuge (Beckman, Inc.). The supernatant solutions were centrifuged at 15,800xg for 15 min at 4°C in a microcentrifuge (Model 5042 ...
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bioRxiv - Plant Biology 2020Quote: ... The loaded density gradients were centrifuged at 33,000 g for 14.5 h at 4°C using a six-position SW41 Ti rotor (Beckman Coulter, Kerfeld, Germany). The positions of ribosome complexes varied slightly with each centrifugation run that comprised a set of six samples ...
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bioRxiv - Bioengineering 2020Quote: ... Ultracentrifugation was performed at 100,000 x g at 4 C for 45 min in an Optima MAX-XP ultracentrifuge (Beckman Coulter, Inc.). Supernatant was discarded ...
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bioRxiv - Biochemistry 2020Quote: ... in BC300N buffer and centrifuged for 4hr at 55,000rpm at 4°C in a TLS-55 rotor (with Beckman 356860 adaptors). 40µl fractions were collected by pipetting from the top ...
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bioRxiv - Biophysics 2021Quote: Purified 3C DNA from each sample was sonicated to 200-400 bp with a Soniprep 150 probe sonicator at 4°C and purified with a standard Ampure XP Bead protocol (Beckman Coulter) using a 1/1.5 DNA to bead ratio ...
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bioRxiv - Biophysics 2021Quote: ... transferred to 15 mL conical tube and centrifuged at 233 x g for 5 min at 22 °C using a swinging bucket rotor (Beckman Coulter). Pelleted cells were resuspended and washed once with 1x PBS (-CaCl2 ...
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bioRxiv - Neuroscience 2020Quote: ... The supernatant was then collected and subjected to ultracentrifugation at 100,000 x g for 70 min at 4°C using an Optima XPN-80 ultracentrifuge with SW 32 Ti swinging rotor (Beckman Coulter). Pellets were washed in PBS 1X and EVs were subjected to an additional centrifugation at 100,000 x g for 30 min at 4°C using an Optima MAX ultracentrifuge ...
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bioRxiv - Neuroscience 2020Quote: ... centrifuged at 100,000 g for 70 min at 4°C using an Optima XPN-80 ultracentrifuge with SW 32 Ti swinging rotor (Beckman Coulter) and then processed for further analyses.
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bioRxiv - Plant Biology 2021Quote: ... Syringes were placed inside 50-mL tubes and centrifuged for 20 min at 700g with slow acceleration (4°C, JA-14 rotor, Avanti J-20 XP Centrifuge; Beckman Coulter). The apoplastic wash fluid was then filtered through a 0.22 μm membrane and centrifuged at 10,000g for 30 minutes to remove any remaining large particles ...
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bioRxiv - Biochemistry 2021Quote: ... 2 mM DTT) and centrifuged for 2 hours and 15 minutes at 39000 rpm at 4°C (Beckman Coulter TLS55 rotor). Fractions corresponding to the large mitochondrial subunit were collected and subjected to buffer exchange (25 mM HEPES-KOH pH = 7.5 ...
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bioRxiv - Cell Biology 2020Quote: ... The first PCR reaction was performed for 15 cycles with 60 °C annealing and 30 s elongation and then purified with AMPure XP PCR beads (Beckman Coulter). The second PCR was performed for 15 cycles for wild type-specific and for 21 cycles for tag-specific amplicons respectively using 60°C annealing and 30 s elongation ...
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bioRxiv - Molecular Biology 2021Quote: ... and spung for 20hrs at 151,000 x g (rav) at 4°C in a swinging bucket rotor (Beckman SW-41 Ti). 12 1ml fractions were taken off the gradient and incubated for 10min at RT with 100μl 0.15% deoxycholine ...
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bioRxiv - Cell Biology 2021Quote: ... or Lin−Sca-1+c-Kit+CD150+CD48−CD34− (CD34−HSC) and Lin−Sca-1+c-Kit+ (LSK) cells were sorted on MoFlo Astrios EQ (Beckman Coulter). Quantification of CD71 ...
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bioRxiv - Microbiology 2021Quote: ... and cytoplasmic membranes were precipitated by ultracentrifugation for 45 min at 55,000 rpm and 4°C (Beckman Coulter rotor TLA 55). Membrane fractions resuspended in resuspension buffer (750 mM aminocaproic acid ...
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bioRxiv - Biophysics 2021Quote: ... The crude lysate was sonicated on ice and clarified by centrifugation for 30 min at 4°C and 35,000 rpm in a Beckman L90K Optima and 50.2 Ti rotor (Beckman, Brea, CA, USA). The clarified lysate was applied to a HisTrapHP column (Cytiva ...
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bioRxiv - Biophysics 2021Quote: ... and the fractions containing the protein of interest were combined and concentrated to 2mL at a final concentration of 3.85 mg/mL by repeated centrifugation in an Amicon Ultra Centrifugal Filter at 4k rpm for 5 min at 4 °C (Beckman SX4250). The concentrated protein was incubated overnight with TEV (1:50 mass ratio ...
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bioRxiv - Cell Biology 2021Quote: ... and loaded onto of a step-wise sucrose gradient as described by the manufacturer and subjected to ultracentrifugation at 150,000 xg for 4 h at 4 °C using SW50.1 rotor (Beckman Coulter, Mississauga, ON). Fractions were then collected and subject to denaturation with Laemmli buffer until further use.
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bioRxiv - Biophysics 2020Quote: ... and clearance was determined by monitoring Optical Density (DO) at 325 nm and 24°C in a Beckman Coulter DTX 880 Microplate Reader (Beckman, CA). Curves were adjusted by fitting to a single exponential decay y = y0 + ae−bx.