Labshake search
Citations for Beckman :
901 - 950 of 1869 citations for 2E 4E 2 4 Octadien 1 ol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Pseudotypes and VLPs were then concentrated by ultracentrifugation (20,000 RPM, 4 ºC, 1.5h, Beckman Coulter Optima XPN-100, SW32Ti rotor) through a 20% (w/v ...
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bioRxiv - Microbiology 2022Quote: ... 20 mL of the filtrate was then ultracentrifuged at 150,000 × g at 4°C for 3 h (Beckman Coulter, USA) through a 20% (w/v ...
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bioRxiv - Microbiology 2022Quote: ... at 20 KPSI at 4°C and then centrifuged at 19 000 RPM in a JA-25.50 rotor (Beckman Coulter) for 30 minutes at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... using 10-40 % sucrose gradients in buffer A after 3 hours of centrifugation at 35,000 rpm and 4 °C in SW41 rotor (Beckman Coulter). To isolate ribosomes for structural analysis ...
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bioRxiv - Neuroscience 2023Quote: ... followed by ultracentrifugation at 100,000 x g for 60 minutes at 4°C (SW 41 Ti Rotor, Beckman Coulter Inc). For size-exclusion chromatography (SEC ...
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bioRxiv - Neuroscience 2022Quote: ... trypsin-dissociated cells on 96-well plates were centrifuged at 1,000g for 5 min at 4°C using a swinging-bucket rotor for microplates (Beckman S6096) in an Allegra X-30R centrifuge (Beckman) ...
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SARS-CoV2 associated secretion of nanoLuciferase reports on virus and Virus-Like Particle productionbioRxiv - Molecular Biology 2022Quote: ... the samples were pelleted through a 20% sucrose in 120 mM HEPES cushion at 100,000xg for 2hrs at 4 °C with 45 Ti or 70 Ti fixed-angle rotor (Beckman-Coulter) depending on the volume of the sample ...
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bioRxiv - Molecular Biology 2022Quote: ... The gradients were ultra-centrifuged for 3 h at 175,000g at 4°C in a SW41Ti rotor (Beckman-Coulter, USA) and analyzed by measuring the absorbance continuously at 260 nm using a piston gradient fractionator (Biocomp Instruments) ...
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bioRxiv - Molecular Biology 2022Quote: ... and spun at 40,000 rpm for 3 hours at 4°C using a SW 41 Ti Swinging-Bucket Rotor (Beckman Coulter). Sucrose gradient fractionation was performed by using a Brandel Density Gradient Fractionation System ...
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bioRxiv - Molecular Biology 2022Quote: ... and the sucrose cushion was ultra-centrifuged for 1.5 hours at 80000 rpm and 4°C in TLA-100 rotor (Beckman Coulter). The pellet was resuspended in 100 µL of buffer F (50 mM HEPES-KOH pH 7.4 ...
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bioRxiv - Cell Biology 2024Quote: ... The step gradients were centrifuged for 5 h at 132.000 x g at 4 °C (SW60 Beckman and Coulter, # 335649). Subsequently ...
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bioRxiv - Molecular Biology 2024Quote: ... the rest was loaded on top of sucrose gradient and ultracentrifuged (30000 rpm, 4°C, 18h, stop DECEL, slow, SW40/1Ti swinging bucket rotor, Optime L-90K, Beckman). 24 fractions were taken from top to bottom of the tube as 500 µL aliquots and precipitated by incubation with 50 µL of 0.15% sodium deoxycholate for 10 min at RT ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were centrifuged at 30,000 xg for 45 min at 4 °C using a SW41 Ti swinging bucket rotor (Beckman Coulter) before the supernatant was decanted ...
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bioRxiv - Neuroscience 2024Quote: ... The homogenate was centrifuged (45 min, 16 000 rpm, 4°C, JLA 16.250 rotor, Beckman-Coulter Centrifuge Avanti J25-01) and the supernatant subjected to Ni-NTA affinity purification (HIS-Select Nickel Affinity Gel (Sigma Aldrich)) ...
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bioRxiv - Biochemistry 2024Quote: ... The homogenate was clarified by centrifugation for 30 min at 70,560 × g at 4°C in an Optima XPN Ultracentrifuge (Beckman Coulter) using a Ti-45 rotor ...
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bioRxiv - Bioengineering 2023Quote: ... The final supernatant was centrifuged at 100,000g at 4°C for 70 min using an ultracentrifuge (Optima MAX-XP Tabletop Ultracentrifuge, Beckman Coulter). The pellet was either used immediately or stored dry at −80°C ...
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bioRxiv - Biochemistry 2023Quote: ... and the mixtures were then layered under a 20-40% iodixanol discontinuous gradient before to be centrifuged at 260,000 x g for 16h at 4°C using an SW41 Ti rotor (Beckman Coulter). After spinning ...
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bioRxiv - Genomics 2023Quote: ... 1x protease inhibitor cocktail EDTA-free) by spinning at 38,000 rpm at 4 °C in a SW-41 Ti rotor (Beckman Coulter). Monosome pellets were resuspended in 400 µL lysis buffer and rocked overnight ...
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bioRxiv - Biochemistry 2024Quote: ... The protein samples were carefully applied to the gradient and centrifuged for 16 h at 130,000 g at 4 °C (Optima XPN-80 Ultracentrifugation from Beckman Coulter). 200 µl fractions were taken from the top of the gradient and the glutaraldehyde quenched with 20 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... The homogenate was clarified by centrifugation for 30 min at 70,560 × g at 4°C in an Optima XPN Ultracentrifuge (Beckman Coulter) using a Ti-45 rotor ...
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bioRxiv - Biophysics 2024Quote: ... and subjected to ultracentrifugation at 384,492□g for 45□min at 4□°C (Beckman Coulter Optima MAX, TLA-100 rotor), stored at −80 °C after flash-frozen with liquid nitrogen for crystallization trials ...
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bioRxiv - Biophysics 2023Quote: ... Centrifugation was performed at 154,300 × g for 24 h at 4◦C (SW 41 Ti swinging-bucket rotor, Beckman Coulter). The separated bands were collected dropwise from the bottom of the tube ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were cleared by centrifugation at 25 000 rpm for 45 min at 4°C in a Ti45 rotor (Beckman). Supernatant was incubated with 5 ml of Glutathione Sepharose 4B beads slurry (Cytiva ...
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bioRxiv - Neuroscience 2022Quote: ... The homogenised mixture then underwent ultracentrifugation at 200,000 g for 110 min at 4°C with a SW-41 Ti rotor (Beckman Coulter) and transferred the supernatant into a Slide-A-Lyzer™ G2 Dialysis Cassettes 2K MWCO (Thermo ...
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bioRxiv - Molecular Biology 2023Quote: 8 mL of viral supernatant on a 3 mL of 20% sucrose cushion were ultracentrifugated for 3 h at 100,000 x g at 4°C using an Optima L80-XP instrument (Beckman Coulter). After ultracentrifugation ...
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bioRxiv - Systems Biology 2023Quote: ... Gradients were centrifuged at 4°C for 17 h at 100,000 g using an L7 ultracentrifuge with an SW28 swing out rotor (Beckman Coulter). Fractions (F ...
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bioRxiv - Synthetic Biology 2023Quote: ... The gradients were ultra-centrifuged at 41,000 rpm for 3 hours at 4°C (Optima L-80 XP ultracentrifuge (Beckman-Coulter)) ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was centrifuged at 300,000×g at 4°C for 3 h in a TLA-110 fixed angle rotor (k-factor 13) (Beckman Coulter). The pellet was resuspended in 10 µL PBS of which 3 µL was adsorbed onto glow-discharged 2 nm carbon layer coated Quantifoil R1.2/1.3 400 mesh gold grids ...
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bioRxiv - Cell Biology 2023Quote: ... The iodixanol density gradients were centrifuged at 100,000 x g at 4◦C for 18 hours in SW41Ti rotor (Beckman Coulter). Ten fractions (F1 ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were lysed by sonication and the lysate was clarified at 150,000 rcf for 30 min at 4°C using a Type 45 Ti Rotor (Beckman Coulter). The lysate was passed three times though 2mL of pre-equilibrated Ni-NTA Agarose beads (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were lysed by sonication and the lysate was clarified at 150,000 rcf for 30 min at 4°C using a Type 45 Ti Rotor (Beckman Coulter). The lysate was passed three times through 2 mL of pre-equilibrated Ni-NTA Agarose beads (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was clarified at 150,000 rcf for 45 min at 4°C using a Type 45 Ti Rotor (Beckman Coulter). The supernatant was incubated with 3 mL IgG Sepharose 6 Fast Flow beads (Cytiva ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was clarified at 150,000 rcf for 30 min at 4°C using a Type 45 Ti Rotor (Beckman Coulter). The supernatant was incubated with 3 mL IgG Sepharose 6 Fast Flow beads (Cytiva ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was clarified at 503,000 rcf for 45 min at 4°C using a Type 70 Ti Rotor (Beckman Coulter). The supernatant was incubated with 3 mL IgG Sepharose 6 Fast Flow beads (Cytiva ...
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bioRxiv - Molecular Biology 2023Quote: ... then pelleted at 4°C and 4,300 x g for 30 min in an Allegra X-14R centrifuge (Beckman Coulter) equipped with a swinging bucket SX4750 rotor ...
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bioRxiv - Microbiology 2023Quote: ... Each tube was centrifuged for 3 hours at 150’000 x g (4°C) using an Optima XPN-80 ultracentrifuge (Beckman Coulter) equipped with a SW 32 Ti swinging bucket (369650 ...
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bioRxiv - Cell Biology 2023Quote: ... The obtained supernatant was layered onto 30 w/v% sucrose solution in ultracentrifugation tubes and subjected to ultracentrifugation at 100,000 × g for 3 h at 4°C using SW32Ti rotor (Beckman courter). The collected sucrose solutions were mixed with PBS and washed by ultracentrifugation at 100,000 × g for 1 h ...
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bioRxiv - Systems Biology 2023Quote: ... The urine was centrifuged at 20,000 x g for 30 minutes at 4°C (k-factor 1,790) in an Optima XPN-80 ultracentrifuge (Beckman Coulter) equipped with a SW32 Ti swinging bucket rotor (Rmin 67 ...
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bioRxiv - Plant Biology 2023Quote: ... Approximately 250 µl of crude EV sample resuspended in 20 mM Tris-HCl pH 6.0 was slowly dispensed on top of the column and centrifuged at 100,000 x g for 17 h at 4 ⁰C (SW 41 Ti rotor, Beckman Coulter; OptimaTM L-90K, Beckman Coulter). After the ultracentrifugation ...
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bioRxiv - Cell Biology 2023Quote: ... 355647) and centrifuged at 35,000 rpm (100,000xg) for 40min at 4°C (Fixed-angle rotor, Beckman Coulter Optima Max Unltracentrifuge). 100K pellet (sEVs ...
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bioRxiv - Cell Biology 2023Quote: ... the post-nuclear supernatant was centrifuged for 30 min at 100,000 x g at 4°C using a S120AT2 rotor (Beckman Coulter). The resulting pellet containing the membranes was resuspended in SDS sample buffer (see below).
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bioRxiv - Neuroscience 2023Quote: ... The gradients were centrifuged for 18 h at 100,000 xg at 4 °C in a SW 40 rotor (Beckman Coulter). 500 µl fractions were collected from the top of the ultracentrifuge tubes and analyzed by WB analysis.
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bioRxiv - Cell Biology 2022Quote: ... Lysates were cleared by centrifugation for 30 min at 4°C at 18,000 rpm (SS-34 rotor; Beckman-Coulter, USA).
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bioRxiv - Cell Biology 2023Quote: ... down to 6 ml and ultracentrifuged at 10,000xg for 16 min at 4°C in MLA-80 rotor (Beckman Coulter). The pellet was resuspended and washed with PBS at the same conditions (volume ...
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Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelopebioRxiv - Cell Biology 2023Quote: ... The whole cell homogenate was layered on top of 2 ml shelf of 0.8 M sucrose in HB and centrifuged at 2000 rpm for 10 min at 4°C in a JS5.2 rotor (Beckman Coulter) to yield a low speed nuclear pellet and a postnuclear supernatant ...
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bioRxiv - Bioengineering 2023Quote: ... The final supernatant was centrifuged at 100,000g at 4°C for 70 min using an ultracentrifuge (Optima MAX-XP Tabletop Ultracentrifuge, Beckman Coulter). The pellet was either used immediately or stored dry at −80°C.
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bioRxiv - Biochemistry 2023Quote: ... The reaction mixture was applied onto the top of the gradient solution and was centrifuged at 41,000 rpm at 4 °C for 17 hrs using an SW41 Ti rotor (Beckman Coulter). The gradient was fractionated using a Piston Gradient Fractionator (BioComp) ...
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bioRxiv - Cell Biology 2023Quote: ... and then centrifuged for 150 min at 39,000 rpm and 4°C in an Optima L-100XP Ultracentrifuge (Beckman-Coulter) using a SW41Ti rotor ...
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bioRxiv - Biophysics 2023Quote: ... at 4°C until equilibrium was reached using an An Ti 60 rotor in an XL-I analytical ultracentrifuge (Beckman), run using ProteomeLab XL-A/XL-I (version 6.2) ...
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bioRxiv - Genomics 2023Quote: ... the supernatant was cleared by two rounds of centrifugation at 20,000 ×g (10 min, 4 °C, JA-20 rotor, Avanti J-26 XP, Beckman Coulter) followed by centrifugation at 31,900 ×g (10 min ...