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Citations for Beckman :
1151 - 1200 of 1869 citations for 2E 4E 2 4 Octadien 1 ol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... density gradient (35 to 0% in PBS) and ultracentrifuged at 200,000 × g for at least 12 hours at 4 °C (Beckman Optima XL-100K). The corresponding clear yellow band was collected ...
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bioRxiv - Microbiology 2023Quote: ... The lysate was clarified by centrifugation at 5500 rpm for 20 min at 4°C (AllegraTM 21R Centrifuge, Beckman Coulter, CA, USA). The sample was then loaded into a HisTrap HP 5 mL column (Cytiva ...
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bioRxiv - Microbiology 2023Quote: ... Cells were incubated further overnight at 20°C and subsequently harvested by centrifugation (Beckman rotor 8.1000, 5000 rpm, 15 min, 4°C). The pellet was resuspended in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... then spun at 100,000 x g for 30 min at 4°C in an ultracentrifuge (Beckman OPTIMA TLX with TLA-100.3 rotor). The supernatant was saved and the pellet went through a series of similar extractions using High-Salt Triton Buffer (High-Salt Buffer ...
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bioRxiv - Neuroscience 2023Quote: ... The supernatants were then combined and centrifuged at 17,000g for 10min at 4°C in a Beckman SW41-Ti rotor (Beckman Coulter, Brea, CA). The supernatant was saved as the cytosolic (CYT ...
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bioRxiv - Cell Biology 2023Quote: Myf5High and Myf5Low MuSCs were isolated from a pool of 4 male and 4 female mice and repeated in two independent experiments and total RNA was extracted using Agencourt RNAdvance tissue Kit (Beckman Coulter, #A32646) with a quality score of RQN 9.3 to 10 ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was again centrifuged at 100000 g for 45 min at 4°C (Beckman Optima™ MAX-XP Rotor MLS-50). After filtering the supernatant through a 0.22 μm syringe filter ...
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bioRxiv - Cell Biology 2022Quote: ... One milliliter of concentrated conditioned medium was pipetted on top of the gradient and samples were centrifuged for 18 h at 100,000 ×g and 4 °C using a SW 32.1 Ti rotor (Beckman Coulter, Fullerton, California, USA). Fractions of 1 mL were collected from the top and EV-rich fractions 9 and 10 (corresponding to a density of 1.10-1.12 g/mL ...
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bioRxiv - Biochemistry 2023Quote: ... Cultures were then recovered by centrifugation (4,000 x g, 4 °C, 40 min in a Beckman Avanti JXN-26 (rotor: JLA 8.1)) and pellets resuspended in approximatively 30 mL of lysis buffer (20 mM HEPES ...
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bioRxiv - Molecular Biology 2024Quote: ... ADEV isolation was performed by ultracentrifugation at 100,000 × g for 14 h at 4 °C (swing bucket rotor on minimum acceleration/break setting, SW 32Ti Beckman Coulter, Brea, CA), and ADEVs were resuspended in 200μL PBS and stored at −20°C until used16,41 ...
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bioRxiv - Cell Biology 2024Quote: ... All centrifugation steps were done at 4°C and the last two steps were performed using Optima XE-90 ultracentrifuge (Beckman Coulter, A94471) with rotor SW32Ti (Beckman Coulter ...
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bioRxiv - Cell Biology 2024Quote: ... and cells were isolated by centrifugation at 500 g for 5 min at 4°C followed by being treated with a red blood lysis buffer (Beckman Coulter, USA). After washing with PBS ...
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bioRxiv - Microbiology 2024Quote: ... Saliva was collected by expectoration into tubes on ice followed by centrifugation for 10 minutes at 4°C at 4000 x g (Beckman SX4750 rotor) to remove large debris and bacterial aggregates ...
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bioRxiv - Biochemistry 2023Quote: ... by centrifugation at 80,000 × g for 30 min at 4°C using a TLS-55 rotor (Beckman Coulter Inc., Miami, FL, USA). Crista membranes were identified and removed based on fluorescence under a UV lamp ...
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bioRxiv - Cell Biology 2023Quote: ... Following centrifugation at 131,300 × g for 24 hr at 4 °C using a MLS-50 Swinging-Bucket rotor (Beckman Coulter, Brea, CA) to sediment protein complexes ...
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bioRxiv - Cell Biology 2022Quote: ... The resulting supernatant was centrifuged at 10,000 x g for 30 minutes to remove any remaining debris and then ultra-centrifuged at 118,000 x g for 70 minutes at 4°C with a fixed-angle rotor (Ti-70, Beckman Coulter, Brea, CA). The pellet was washed with sterile 1X PBS and subjected to another cycle of ultracentrifugation at 118,000 x g for 70 minutes at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... The supernatants were further centrifuged at 10,000 × g for 30 min at 4°C to remove remaining cell debris and at 100,000 × g (Type 90 Ti Rotor, Beckman, Brea, CA, USA) overnight at 4°C to remove exosomes ...
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bioRxiv - Biochemistry 2022Quote: ... Triton-X was added to the lysate to a final concentration of 0.1% and clarified by spinning at 15,000 RCF at 4°C for 45 minutes (Beckman Coulter JA-20 rotor). The supernatant was loaded onto a 5 mL HisTrap™ FF crude column (GE Healthcare ...
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Isolation and characterization of a novel phage of Vibrio parahaemolyticus with biocontrol potentialbioRxiv - Microbiology 2022Quote: ... for purification and centrifuged at 200,000 × g at 4°C for 24 h using an Optima L-100 XP ultracentrifuge (Beckman Coulter, CA, USA). The purified phage particles were dialyzed through 30-kDa superfilters (Millipore ...
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bioRxiv - Neuroscience 2023Quote: ... re-suspend in the homogenization buffer and centrifuged at 100,000 x g for 40 min at 4°C (Beckman SWTi41 swing rotor). The pellet containing the growth cone were re-suspended in 50 μl of the homogenization buffer ...
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bioRxiv - Cell Biology 2023Quote: ... was next subjected to ultracentrifugation at 120,000 × g for 4 h in a SW 28 Ti Swinging-Bucket Rotor (Beckman Coulter, Fullerton, CA) to sediment small EVs ...
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bioRxiv - Biochemistry 2023Quote: ... Triton-X was added to the lysate to a final concentration of 0.1% and clarified by spinning at 15,000 RCF at 4°C for 45 minutes (Beckman Coulter JA-20 rotor). The supernatant was loaded onto a 5 mL HisTrap™ FF crude column (GE Healthcare ...
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bioRxiv - Biophysics 2024Quote: ... Samples were centrifuged at 24,000 rotations per minute using an S-28 rotor at 4◦C in an Optima XL-100K Ultracentrifuge (Beckman Coulter, Brea, CA). The supernatant was decanted ...
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bioRxiv - Developmental Biology 2024Quote: ... for 30 min at 4°C in the dark followed by flow cytometry analysis on a CytoFlex 26 (Beckman Coulter, Indianapolis, IN) in PE-A and PerCP-A channels ...
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bioRxiv - Molecular Biology 2024Quote: ... was loaded on top of the gradient and spun at 4°C for 18 hr at 30,000 rpm (Beckman, Rotor SW-41Ti). Samples were fractionated with absorbance optics (λ = 260 nm ...
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bioRxiv - Neuroscience 2024Quote: ... This gradient was centrifuged for 30 minutes at 72,000 x g at 4°C (33,000 rpm, TLS-55 rotor, Beckman Coulter Optima TLX Ultracentrifuge). After centrifugation ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: The number of μPL per PVA template and size distribution were derived via a Multisizer 4 COULTER particle counter (Beckman Coulter, CA). Briefly ...
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bioRxiv - Neuroscience 2024Quote: ... The suspension was then centrifuged at 2500 × g for 30 min at 4 °C and the supernatant was transferred to an ultra-centrifugation tube (Quickseal Tubes, Beckman Coulter #342414). AAV purification was performed by ultra-centrifugation over a discontinuous Iodixanol density gradient (OptiPrep Density Gradient Medium ...
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bioRxiv - Plant Biology 2024Quote: ... The fluid was centrifuged at 1,000 x g for 10 minutes at 4 C using an Allegra X-30R centrifuge (Beckman Coulter Life Sciences). The resulting supernatant was centrifuged at 200,000 x g for 30 minutes at 4 °C using a Beckman Optima Ultracentrifuge with TLA110 rotor (Beckman Coulter Life Sciences) ...
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bioRxiv - Plant Biology 2024Quote: ... The resulting supernatant was centrifuged at 200,000 x g for 30 minutes at 4 °C using a Beckman Optima Ultracentrifuge with TLA110 rotor (Beckman Coulter Life Sciences). The pellet was resuspended in 30 µL of cold ddH20 and stored in −80 °C until further use.
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bioRxiv - Biochemistry 2024Quote: ... Purified nucleosome arrays were subsequently isolated on 5-40% w/v sucrose gradients in 0.5X TE by centrifuging the gradients at 39,000 rpm and 4°C for 8 h in a SW41 Ti rotor (Beckman Coulter, Brea, CA). After centrifugation ...
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bioRxiv - Cell Biology 2024Quote: ... The crude pellets from MSC-CM and HDF-CM after the first-round 100,000 × g ultracentrifugation were washed with PBS at 100,000 × g and 4 ℃ for 45 min using Optima™ MAX-TL Ultracentrifuge (Beckman Coulter, USA) and TLA-120.2 Rotor (Beckman Coulter ...
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bioRxiv - Genetics 2021Quote: ... and samples were evaluated 2 minutes later with a Cytomics FC500 flow cytometer (Beckman Coulter, Brea, CA, USA) AO was excited with a 488 nm argon laser ...
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bioRxiv - Genomics 2020Quote: ... The PCR products were purified by using 1.3X Agencourt AMPure XP beads (2 or 3 cycles; Beckman Coulter) and evaluated by using an Agilent High Sensitivity DNA Kit on an Agilent 2100 Bioanalyzer (Agilent) ...
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bioRxiv - Cancer Biology 2020Quote: ... concentrated for 2 h by ultracentrifugation in a swing-out rotor SW-32Ti (Beckman Coulter, Brea, US-CA) at 26000 g ...
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bioRxiv - Microbiology 2023Quote: ... The filtrate was subjected to ultracentrifugation at 200,000xg for 2 h (Optima L-100 XP ultracentrifuge; Beckman Coulter). Supernatants were discarded ...
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bioRxiv - Microbiology 2024Quote: ... (The filtrate was subjected to ultracentrifugation at 100,000g for 2 h (Optima L-90 K ultracentrifuge; Beckman Coulter). The supernatant was discarded and the pellet was washed with sterile PBS and filtered through a sterile 0.2 μm-pore polyvinylidene difluoride (PVDF ...
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bioRxiv - Cell Biology 2022Quote: ... Lentiviral particles were then concentrated by centrifugation for 2 hours at 20,000 rcf (Beckman Coulter, Optima L-100K) in round bottom polypropylene tubes (Beckman Coulter ...
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bioRxiv - Cell Biology 2022Quote: Total RNA of Caco-2 cells and human ileum organoids was extracted using RNAdvance Tissue kit (Beckman Coulter) and total RNA of mouse organoids was isolated using Quick-RNA Miniprep Plus Kit (Zymo Research) ...
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bioRxiv - Physiology 2024Quote: ... was analysed via a sequential 2-step immune-enzymatic assay (inter-assay CV 5.2-7.8%) according to manufacturer’s instructions (Beckman Coulter) and follicle stimulating hormone (FSH ...
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bioRxiv - Immunology 2024Quote: ... The whole cell lysate was sonicated for 2 min and the cell lysate was centrifuged for 60 min at 20,000 rpm (JA 25.50 rotor, Beckman). The recombinant P72 protein in the supernatant were collected and applied to the anti-HA Magnetic Beads (MCE ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 μl of the sample pre- and post-bead clean-up (SPRIselect beads, Beckman Coulter, cat. no. B23317) were run in the TapeStation D1000 as a quality control to check peak size ...
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bioRxiv - Cell Biology 2024Quote: ... MKs were subsequently fixed with 2% paraformaldehyde and activation measured using the CytoFLEX flow cytometer apparatus (Beckman Coulter). Normalized mean florescence intensity (MFI ...
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bioRxiv - Genomics 2021Quote: ... then re-purified with SPRI beads with 1:1 ratio (Beckman Coulter, B23317), and eluted in 25 µl of water (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... by ultra-centrifugation in a Beckman L7-65 ultracentrifuge at 25,000 rpm for two hours at 4°C using a Beckman SW28 rotor (Beckman Coulter, Brea, CA, USA). Supernatants were aspirated off and the pellets were re-suspended in PBS (pH 7.4) ...
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bioRxiv - Microbiology 2020Quote: ... by centrifugation in a Beckman L7-65 ultracentrifuge at 25,000 rpm for 2h at 4°C using a Beckman SW28 rotor (Beckman Coulter, Brea, CA, USA). Supernatants were aspirated and the pellets were re-suspended in PBS (pH 7.4) ...
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bioRxiv - Molecular Biology 2021Quote: ... The centrifugation is performed on a SW60Ti rotor at 40 000 g for 22 minutes at 4°C (Beckman Optima L-90K Ultracentrifuge). The mitochondrial fraction (400µL ...
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bioRxiv - Molecular Biology 2020Quote: ... and plasma was separated by centrifugation (2,000 × g, 20 min, 4 °C) in a Microfuge 20R (cat. no. B30149, Beckman Coulter, Brea, CA, USA). Plasma was transferred to new 1.5 mL microcentrifuge tubes and further clarified by centrifugation (10,000 × g ...
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bioRxiv - Biophysics 2022Quote: ... shortly sonified and centrifuged for 30 min at 100 000 g at 4°C (Centrifuge Beckman Coulter Optima XPN-80, Rotor type 70.1).) ...
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bioRxiv - Biophysics 2022Quote: ... The samples were centrifuged for 30 min at 100 000 g at 4°C (Centrifuge Beckman Coulter Optima XPN-80, Rotor type 70.1). The supernatant ...