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Citations for Beckman :
451 - 500 of 1117 citations for Sulfur Free Cobalt Metallo Organic Standard Co @ 5000 µg g in Hydrocarbon Oil since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... The insolubilized materials were removed by centrifugation at 164,700 g for 30 min in Ti70 rotor (Beckman Coulter). The solubilized proteins were loaded onto a 2-ml Streptactin beads 4FF (Smart-Lifesciences ...
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bioRxiv - Cell Biology 2024Quote: ... the pellet was resuspended and re-centrifuged at 100,000 x g in low-bind 1.5 mL ultracentrifuge tubes using a TLA55 fixed angle rotor (Beckman). The pellet was resuspended in 50 μL of PBS and denatured through the addition of 50 μL 2 x LDS sample buffer containing 100 mM DTT.
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bioRxiv - Cell Biology 2022Quote: ... 10 mM HEPES pH 7.4 and EDTA free protease inhibitor were prepared in polyallomer optiSeal ultracentrifuge tubes (11.2 ml capacity; Beckman Coulter) gradients were allowed to diffuse 1h at 20 °C ...
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bioRxiv - Biophysics 2020Quote: ... GLIC proteoliposomes were collected by centrifugation of the Biobead-free sample at 100,000 rpm in TLA-120.2 rotor (Beckman Coulter) for 1 hr ...
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bioRxiv - Microbiology 2024Quote: The resulting amplicons were then cleaned of free primers and primer dimer species using AMPure XP beads (Beckman Coulter). Sequencing libraries were prepared using the Nextera XT library preparation kit (Illumina ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were then resuspended in 0.1% BSA containing 10 µg/µl Hoechst 33258 and analyzed for senescence in a Cytoflex S cytometer (Beckman Coulter). For liver tissue ...
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bioRxiv - Biophysics 2021Quote: ... the samples were spun in a bench-top Beckman ultracentrifuge at 670,000 g at 20 °C for 30 min (TLA-120.1 rotor, Beckman-Coulter) to pellet F-actin and associated Tpm ...
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bioRxiv - Biochemistry 2021Quote: ... after which the lysate was clarified by centrifugation at 30,000 x g for 60 min at 4 °C in a JA 25.50 rotor in an Avanti J-E centrifuge (Beckman). Nucleic acids in the clarified lysate were precipitated by slowly adding polyethyleneimine with constant stirring in an ice bath to a final concentration of 0.4% ...
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bioRxiv - Cell Biology 2021Quote: ... and diluted up to ∼ 5ml of PBS and followed by centrifugation at ∼150,000×g (38,500 RPM) in an SW-55 rotor (Beckman Coulter). Washed pellet material was then resuspended in 100 µl PBS as in the first centrifugation step and 900 ul of 60% sucrose (in buffer C ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were centrifuged for 20 h at 190,000 x g in a SW-40 Rotor (Beckman Optima L-70 or Beckman Optima L-80 XP ...
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bioRxiv - Cell Biology 2020Quote: The bovine EV-depleted media were obtained by overnight ultracentrifugation at 100,000 × g in a 45Ti rotor (Beckman Coulter), of DMEM supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2020Quote: ... 0.5 mM DTT, 100 μg/ml cycloheximide) and centrifuged (160,000 g, 120 min, 4°C) in an SW41Ti rotor (Beckman). After centrifugation the sediment in the tube went through a UV detector at the wavelength of 254nm and simultaneously the required fractions (>=5 ribosomes ...
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bioRxiv - Cell Biology 2020Quote: ... The sample was centrifuged at 14,000 g for 10 min at 10°C and then loaded in an Optima analytical centrifuge (Beckman) in an An-50Ti rotor pre-equilibrated at 20°C ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by ultracentrifugation at 200000 g for 120 minutes (Type 70 Ti rotor, Optima L-80 XP, Beckman Coulter). The pellet was washed in PBS and collected by ultracentrifugation at 200000 g for 120 minutes [17] ...
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bioRxiv - Cell Biology 2020Quote: ... supernatants were centrifuged at 100,000 × g for 190 min in ultracentrifugation using type 70 Ti rotor (Oprima XL-80K, Beckman) to pellet the EVs (Fig ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were loaded on the top of the gradient and centrifuged at 260,000 × g and 4 °C for 2 h using SW 41 rotor (Beckman). Collection of polysomal fractions were done using Piston Gradient Fractionator (BioComp) ...
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bioRxiv - Microbiology 2021Quote: ... 200mM NaCl was used to top up the 13mL polyethylene tube and the solutions were spun at 27,000×g for 18 hours at 4°C using SW45 Ti rotor (Beckman). 1mL of top fraction ...
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bioRxiv - Biophysics 2021Quote: ... sucrose density gradients and centrifuged at 24,000 × rpm (102,170 × g) for 18 h at 4°C (Sw40Ti rotor, Beckman Coulter). The fractions of the sucrose gradient were applied to SDS-PAGE ...
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bioRxiv - Biophysics 2021Quote: ... The VP2 capsid protein fractions were pooled and subjected to centrifugation at 28,000 rpm (139,065 × g) for 3 h at 4°C (Sw40Ti rotor, Beckman Coulter). The pellet was resuspended in 50 mM Tris (pH 7.4) ...
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bioRxiv - Biophysics 2021Quote: ... The polymerized microtubules were centrifuged for 30 min at 18000 x g in a Microfuge 18 Centrifuge (Beckman Coulter). After centrifugation the pellet was resuspended in BRB80T.
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bioRxiv - Biophysics 2021Quote: ... The polymerized microtubules were centrifuged for 30 min at 18000 x g in a Microfuge 18 Centrifuge (Beckman Coulter). After centrifugation ...
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bioRxiv - Cancer Biology 2021Quote: ... at the bottom as the cushion and centrifuged at 50,000 × g for 2h using an SW32Ti rotor (Beckman Coulter). After centrifugation ...
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bioRxiv - Cell Biology 2021Quote: ... and centrifuged at 97,658 x g for 3 h at 4°C using a SW41Ti rotor (Beckman & Coulter, # 331362). The polysome fractions were collected using the Foxy R1 Fraction Collector and immediately snap frozen with liquid nitrogen and stored at −80 °C ...
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bioRxiv - Cell Biology 2021Quote: ... The lysate was clarified by centrifuging at 183,960 x g for 88 min in Type 70 Ti rotor (Beckman). The clarified supernatant was incubated with 4 mL of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were lysed via sonication (Branson Digital Sonifier) and clarified via centrifugation at 66,000 x g for 30 min in a Type 70 Ti rotor (Beckman) at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... and centrifuged at 40,000 rpm (∼128,000 × g) for 3 h at 4°C using an MLS-5 rotor (Beckman Coulter). Aldolase (158 kDa ...
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bioRxiv - Biochemistry 2020Quote: ... The functional complex was recovered as a jelly-like pellet by centrifugation at 60,000 rpm at 25°C for 2 h in an ultracentrifuge (128,000 g in a TLA 120.2 Beckman rotor) and packed into a 1.9 mm rotor ...
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bioRxiv - Cell Biology 2021Quote: ... then finally at 100,000 g for 4 h at 4 ℃ using an SW28 rotor (Beckman Coulter, Fullerton, CA, USA). The pelleted exosomes were collected then subsequently resuspended in PBS ...
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bioRxiv - Plant Biology 2020Quote: ... 50 μg/ml cycloheximide) and centrifuged for 3 h at 55,000 g and 4 °C in a Type 70 Ti rotor (Beckman). The pellet was dissolved in 1% SDS ...
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bioRxiv - Microbiology 2020Quote: ... and separated in potassium tartrate-glycerol gradient 10–35% for 2 hrs at 175,000 ×g (Beckman SW41 Ti rotor). Purified viral particles were suspended in 10 µg/ml BSA solution or 10 µg/ml CbAE-1 solution and incubated for 1 hr at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... liquid-captured bioaerosols (5 – 10 mL) were centrifuged for 10 minutes at 3000 × g (Allegra X-15R, Beckman Coulter) and resuspended in 200 μL of Middlebrook 7H9 medium supplemented with 100 μM DMN-tre ...
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bioRxiv - Microbiology 2021Quote: ... Filtered supernatants were pelleted at 60,000 g for 16 hours at 4 °C in a SW28 Ti rotor (Beckman), and the resulting pellet was resuspended in TNE buffer (50 mM Tris-HCl [pH 7.4] ...
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bioRxiv - Microbiology 2020Quote: ... Supernatant was transferred to a fresh tube and further clarified by centrifugation at 18,000 x g for 10 min at 4°C in a microfuge (Beckman), and supernatant transferred to a fresh tube.
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bioRxiv - Microbiology 2020Quote: ... Cell lysate was then clarified 200 x g for 10 min at 4°C in an Allegra centrifuge (Beckman). Supernatant was transferred to a fresh tube and further clarified by centrifugation at 18,000 x g for 10 min at 4°C in a microfuge (Beckman) ...
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bioRxiv - Biophysics 2020Quote: ... and centrifuged at 287,582 x g (90,000 rpm) for 1 hr at 4 °C in a TLA100.2 rotor (Beckman, ref. 362046) in 11×34 mm thick-wall polycarbonate ultracentrifuge tubes (Beckman ...
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bioRxiv - Cell Biology 2020Quote: ... Fractions were diluted and washed in PBS for 1h at 100,000 x g in a TLA 110 rotor (Beckman). Fractions were resuspended in 35 ul of PBS ...
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bioRxiv - Cell Biology 2021Quote: ... The lysate was clarified by centrifuging at 66,000 x g for 30 min in Type 70 Ti rotor (Beckman). The clarified supernatant was incubated with 1.5 mL of anti-Flag M2 affinity gel (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... The lysate was clarified by centrifuging at 66,000 x g for 30 min in Type 70 Ti rotor (Beckman). The clarified supernatant was incubated with 2 mL of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences ...
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bioRxiv - Cell Biology 2022Quote: ... The lysate was clarified by centrifugation (183,960 x g, 88 min, 4°C) in a Type 70Ti rotor (Beckman). The supernatant was mixed with 2 ml of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences ...
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bioRxiv - Molecular Biology 2022Quote: ... Gradients were spun for 3 hours at 4° C and 36,000 RPM (221,632 x g) on SW41-Ti rotor (Beckman Coulter). Monosome fractions were isolated from each sucrose gradient with Brandel Density Gradient Fractionator using 1.5 mL/min flow speed and 60% CsCl ...
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bioRxiv - Cell Biology 2022Quote: ... The lysate was clarified via centrifugation (66,000 x g, 30 min, 4°C) in a Type 70 Ti rotor (Beckman). The supernatant was mixed with 1.5 ml of anti-FLAG M2 affinity gel (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... The samples were separated through ultracentrifugation for 1 hr at 200,000 g (spin 3 at 49,000 r.p.m. in Beckman TLS-55 rotor). Top layers of supernatants were removed ...
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bioRxiv - Neuroscience 2022Quote: ... The homogenates were then centrifuged for 40 min at 350,000 g in 500 μL polycarbonate centrifuge tubes (Beckman Coulter) at 4°C in an Optima MAX-XP Ultracentrifuge with a TLA 120.1 rotor (Beckman Coulter) ...
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bioRxiv - Microbiology 2022Quote: ... sonicated using a sonicator (Smurt, Microtec) and ultracentrifuged at 100,000 x g for 60 minutes at 4°C (Optima XE-90, Beckman). Protein concentration of the bacterial membrane suspension was determined with Bio-Rad protein assay (BioRad).
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bioRxiv - Biochemistry 2022Quote: ... by dissolving the yeast pellet in 100 mL wash buffer per 100 g cell pellet and centrifugation (JLA 8.1000 rotor, Beckman) at 5,000 rpm ...
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bioRxiv - Biochemistry 2022Quote: ... 5 mM MgCl 2 and 2 mM DTT and centrifuged at 70,000 x g for 20.5 h at 4 °C in an SW28 rotor (Beckman Coulter). Fractions corresponding to the 40S peak were collected ...
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bioRxiv - Biochemistry 2022Quote: ... Gradients were centrifuged at 42,800 x g for 15 h at 4 °C in an SW28 rotor (Beckman Coulter). Fractions of 1 mL were collected using an AKTA system (Cytiva) ...
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bioRxiv - Microbiology 2022Quote: ... at 4°C for 1.5-2h followed by 180,000 x g (Ti 70i rotor; Beckman Instruments, Inc., Fullerton, CA) at 4°C for 1.5-2h and resuspended in 1mL of phosphate buffered saline (PBS ...
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bioRxiv - Biochemistry 2022Quote: ... The crude extract was centrifuged at 200,000 g for 1 h at 4 °C (Beckman Coulter Optima LE-80K). The membrane pellet was homogenized and solubilized in 20 mM HEPES (pH 7.8 ...
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bioRxiv - Neuroscience 2020Quote: ... The gradient was centrifuged at 200,000 × g for 20 h at 4°C (35,000 rpm with # Optima-XE SW41 Beckman Coulter). The gradient was collected in 2-mL fractions ...