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Citations for Diagenode :
401 - 450 of 582 citations for Copper;5 10 15 20 tetrakis 4 methylphenyl porphyrin 22 24 diide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... heated at 95°C for 10 min and sonicated with the Bioruptor sonicator (Diagenode) for 15 cycles (30 s ON ...
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bioRxiv - Plant Biology 2023Quote: ... on ice for 10 minutes and the lysate was sheared by Bioruptor Plus (Diagenode) for 22 cycles (30 s on/30 s off per cycle) ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by sonication for 10 min (30s on/30s off) using a Bioruptor (Diagenode). For DNA hydrolysis ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were then sonicated (Bioruptor plus, Diagenode, 10 x 30s/30s ON/OFF cycles) and heated up to 37°C for 2 h while shaking (800 rpm ...
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bioRxiv - Genetics 2022Quote: ... per 10 ml of solution and lysed by sonication in Bioruptor (UCD-200, Diagenode) with the following settings ...
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bioRxiv - Neuroscience 2022Quote: ... Sonication (30 s on, 30 s off, 10 min) was performed using Bioruptor (Diagenode). Pre-cleaning of lysate was carried out using blocked Protein G sepharose (ab193259 ...
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bioRxiv - Developmental Biology 2023Quote: ... 0.01% SDS) and sonicated for 10 cycles in a Bioruptor Pico™ sonicator (Diagenode). Cleared supernatants were further diluted with 2.2mL IP dilution buffer ...
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bioRxiv - Genomics 2024Quote: ... Approximately 10 kb sheared DNA by the Megaruptor 3 system (Diagenode, Belgium; Cat. #B06010003) was used for removal of single-strand overhangs at 37°C for 15 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... and 10 mM Tris pH 8.0) and sonicated in a Bioruptor Pico sonicator (Diagenode) for 4 x 30s cycles ...
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bioRxiv - Cell Biology 2024Quote: ... and sonicated for 10 min (30’’ON/30’’OFF, max) using Bioruptor Plus (Diagenode). Lysates were cleared by centrifugation at 20,000 g at 4°C for 30 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... resuspended nuclei were sonicated 5 times 30 sec on followed by 30 sec off using the Bioruptor (Diagenode). Insoluble proteins were removed by centrifugation for 10 min at 14,000 rpm at 4 °C.
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bioRxiv - Cancer Biology 2021Quote: ... Adapter-ligated DNA was subjected to immunoprecipitation with a primary monoclonal antibody against 5-methyl cytosine (Diagenode C15200081) using a previously published protocol [71] ...
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bioRxiv - Genomics 2023Quote: ... 5 × 105 fixed nuclei were sonicated to a 200–500 bp length with the Bioruptor Pico sonicator (Diagenode). H3K27Ac ChIP (Diagenode ...
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bioRxiv - Plant Biology 2024Quote: ... Chromatin sonicated 3 times for 5 min (30 sec on/off intervals) in a Bioruptor® Plus (Diagenode) at the low setting to generate DNA fragments in the range of 200-800 bp ...
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bioRxiv - Cell Biology 2024Quote: ... Chromatin corresponding to 5 μg of DNA was incubated with 2 μg of anti-H3K4me3 (Diagenode; Cat#: C15410003) or anti-H3K9me3 (Diagenode ...
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bioRxiv - Cancer Biology 2020Quote: ... Immunoprecipitation was performed using IP star robot at 4°C (indirect 200µl method, SX-8G IP-Star® Compact Automated System, Diagenode) with an anti-BrdU antibody (10μg ...
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bioRxiv - Molecular Biology 2020Quote: ... with minor modifications: Cells were resuspended in PBS at 4°C after flow cytometry sorting and incubated with ChIP Crosslink Gold (Diagenode #C01019027 ...
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bioRxiv - Cell Biology 2023Quote: ... which were incubated overnight on a rotatory wheel at 4°C with the primary antibodies H4K20me1 (Diagenode; #C15410034; 2 μg), H3K9me3 (Diagenode ...
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bioRxiv - Cancer Biology 2023Quote: ... For protein extractions, the chromatin fraction was sonicated for 4 min (30s-on/30s-off, “high power”) on the Bioruptor Plus (Diagenode). Proteins’ content was quantified with the Thermo Fisher Scientific− BCA Protein Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Genomics 2023Quote: Two micrograms of genomic DNA were sonicated for 6 cycles (6 s on, 90 s off) at 4 °C with a Bioruptor NGS (Diagenode), generating average fragments of 1 kb ...
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bioRxiv - Neuroscience 2023Quote: ... Overnight incubation with primary antibodies at 4°C was performed using the following antibodies: Cas9 (Diagenode, C15200230-100, 1:500), alpha synuclein (Abcam ...
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bioRxiv - Biochemistry 2023Quote: ... sonicated for 10 cycles with 30 ‘’ on and 30 ‘’ off at maximum power at 4 °C using a Bioruptor Pico sonication device (Diagenode). Afterwards ...
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bioRxiv - Molecular Biology 2024Quote: ... and sonicated (6 cycles, high power for 30 s on and 30 s off at 4 °C, Bioruptor NextGen (Diagenode)) ...
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bioRxiv - Genomics 2023Quote: ... Preclearing 30μl of Dynabeads protein A/G for 1 h at 4 °C was followed by incubation with antibodies (H3K27ac, Diagenode, 3C15410196 ...
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bioRxiv - Molecular Biology 2023Quote: ... chromatin was sheared by sonication into 500 – 1000pb fragment (30sec ON/30sec OFF, 6 cycles, 4°C, Diagenode Bioruptor Pico). 10µl was kept aside as the input ...
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bioRxiv - Developmental Biology 2022Quote: ... Samples were centrifuged at 14000rpm for 15mins at 4 oC and precleared using with 4ml of Magnetic A Beads (Diagenode) at 4 oC for 1 hour ...
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bioRxiv - Plant Biology 2022Quote: ... Samples were sonicated for 4 min on a 30 s on/30 s off cycle using a Bioruptor Pico (Diagenode). Subsequent steps were performed as in the Universal Plant ChIP-seq kit protocol ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were then subjected to five cycles (30 seconds on/30 seconds off) of sonication at 4°C on high intensity in a Bioruptor Sonication System (Diagenode) and subsequently cleared by centrifugation at 14,000g for 15 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... Immunostaining was performed overnight at +4°C with primary antibodies (H3K4me2 1:1000; Abcam ab32356) (H3K4me3 1:200; Diagenode C15410003) (H3K4me2 1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... DNA was sonicated at 4°C (program: 6 cycles of 30 s on, 60 s off) on a Bioruptor Plus (Diagenode), achieving a size distribution between 200-1000 nucleotides (nt) ...
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bioRxiv - Neuroscience 2024Quote: ... on ice for 15 minutes and subjected to sonication for 4 separate cycles of 30s on and 30s off cycles at high setting (Bioruptor Plus, Diagenode). The resultant chromatin was centrifuged at 12000 rpm for 30 minutes to remove any debris ...
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bioRxiv - Molecular Biology 2024Quote: ... Nuclei were incubated overnight at 4°C with rotation in wash buffer with rabbit anti-Chd1 (Diagenode 15410334, 1:50). Nuclei were incubated for 1 hour at room temperature with rotation with rabbit anti-H3K4me3 (Sigma-Aldrich 05-745R ...
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bioRxiv - Molecular Biology 2021Quote: ... and 1 mM DTT) and sonicated for 3x 10 seconds using a Bioruptor Pico (Diagenode). Cells were then rotated at 4°C for 20 mins before the lysates were diluted with 500 µl of no salt buffer (50 mM Tris-HCl ...
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bioRxiv - Genomics 2020Quote: ... Chromatin was sheared 10 × 30 sec on/30 sec off with a Bioruptor Pico (Diagenode). Supernatant with chromatin extract was isolated after a 10 minutes centrifugation at 14.000g at 4°C ...
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bioRxiv - Physiology 2021Quote: ... 10% glycerol) supplemented with protease and phosphatase inhibitor cocktails and sonicated in a Bioruptor (Diagenode). Protein lysates were quantified using the Pierce BCA Protein Assay Kit (Thermo Scientific ...
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bioRxiv - Genomics 2022Quote: ... and fragmented by a Bioruptor Pico (10 cycles, 30 sec on/30 sec off; Diagenode). The sonicated solution was diluted (1:5 ratio ...
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bioRxiv - Plant Biology 2024Quote: ... Sheared chromatin (10 cycles, 30 s ON/ 30 s OFF using a Bioruptor® (Diagenode)) extracted from 100 mg material was equally divided between input and samples immunoprecipitated with anti-histone H3 (Merc ...
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bioRxiv - Immunology 2024Quote: ... Samples were boiled for 10 min at 95 °C and sonicated in a Bioruptor (Diagenode) for 10 cycles of 30 sec on/30 sec off ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell lysis and nuclei isolation were carried out in 10 mL of LB1 buffer (Diagenode) by rotating 20 min at 4 C ...
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bioRxiv - Neuroscience 2021Quote: ... DNA was then de-crosslinked and eluted for 4 hours at 65C before being purified using IPure beads (Diagenode Cat# C03010014). The purified DNA was then subjected to sequencing library preparation or qPCR analysis ...
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bioRxiv - Plant Biology 2021Quote: ... The IP of the methylated DNA was performed overnight at 4°C using Protein A Dynabeads coated with anti-5mC (Diagenode, C15200081) or anti-IgG (Diagenode ...
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bioRxiv - Cancer Biology 2020Quote: ... samples were incubated by rotating overnight at 4°C with primary antibody or irrelevant IgGs and then incubated with protein-A agarose beads (Diagenode; C03020002) previously blocked with 0.5% BSA for 3 hr at 4 °C with rotation ...
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bioRxiv - Genomics 2021Quote: ... pH 7.4) aided by homogenization by passing the cells through various gouge needles and sonication using the Bioruptor® sonication system (Diagenode) for 15 cycles of 30 s ON / 30 s OFF at high setting ...
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bioRxiv - Molecular Biology 2024Quote: ... for a total volume of 100 μL and sonicated (4 cycles of 20sec/30sec (on/off time)) using a Bioruptor (Pico II, Diagenode, BE). The successful generation of fragments with a mean fragment size of 400 nucleotides was confirmed using a Bioanalyzer as per manufacturer’s instructions (Agilent ...
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bioRxiv - Genomics 2023Quote: ... Preclearing 30μl of Dynabeads protein A/G for 1 h at 4 °C was followed by incubation with antibodies (H3K27ac, Diagenode, 3C15410196; H3K4me3, Diagenode 3C15410003). Reverse-crossed IP sample were pulled down with streptavidin C1 beads (Life Technologies) ...
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bioRxiv - Biochemistry 2023Quote: Immunoprecipitation was performed using the IP star robot at 4°C (indirect 200 µl method, SX-8G IP-Star® Compact Automated System, Diagenode) with an anti-BrdU antibody (10 μg ...
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Flaviviruses alter endoplasmic reticulum-mitochondria contacts to regulate respiration and apoptosisbioRxiv - Microbiology 2023Quote: Membranes disruption was carried by sonication at 4 °C (2x10 min, 30 sec on, 30 sec off, high setting, Diagenode Bioruptor). Extracts were cleared by centrifugation (15,000 rpm ...
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bioRxiv - Neuroscience 2021Quote: ... the adaptor-ligated DNA was used for immunoprecipitation (IP) with a mouse monoclonal anti-5-hydroxymethylcytosine antibody (Diagenode, C15200200). For this ...
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bioRxiv - Microbiology 2021Quote: ... Cells were lysed by sonication (5 cycles, each 30 s on and 30 s off) using a Biorupter (Diagenode). A centrifugation step (at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... High Molecular Weight Genomic DNA (5 µg) was sheared with the 40 kb program using a Diagenode Megaruptor (Diagenode) to generate DNA fragments of approximately 30 kb ...